FlyBase Gene Report: Dmel\Mt2

General Information

Symbol

Dmel\Mt2

Species

D. melanogaster

Name

Methyltransferase 2

Annotation Symbol

CG10692

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028707

Gene Model Status

Current

Stock Availability

7 publicly available

Enzyme Name (EC)

DNA (cytosine-5-)-methyltransferase (2.1.1.37)

Gene Summary

Methyltransferase 2 (Mt2) encodes a (cytosine-5) tRNA methyltransferase. The modification protects tRNAs against endonucleolytic cleavage and contributes to stress resistance, protein translation and small RNA-mediated gene regulation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Dnmt2, dDnmt2, DNMT, DNA methyltransferase 2

Key Links

Genomic Location

Cytogenetic map

33C4-33C4

Sequence location

2L:12,096,525..12,097,687 [+]

Recombination map

2-46

RefSeq locus

NT_033779 REGION:12096525..12097687

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables DNA (cytosine-5-)-methyltransferase activity

inferred from direct assay

(Tang et al., 2003)

inferred from mutant phenotype

(Phalke et al., 2009)

enables RNA binding

inferred from mutant phenotype

(Genenncher et al., 2018)

enables tRNA binding

inferred from mutant phenotype

(Genenncher et al., 2018)

enables tRNA methyltransferase activity

inferred from mutant phenotype

(Schaefer et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables methyltransferase activity

inferred from sequence or structural similarity with MGI:MGI:1274787

(Hung et al., 1999)

Biological Process (12 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from mutant phenotype

(Lin et al., 2005)

involved_in DNA methylation involved in embryo development

inferred from mutant phenotype

(Kunert et al., 2003)

involved_in DNA methylation

inferred from mutant phenotype

(Phalke et al., 2009)

inferred from direct assay

(Tang et al., 2003)

involved_in heterochromatin assembly

inferred from mutant phenotype

(Phalke et al., 2009)

involved_in negative regulation of transposition

inferred from mutant phenotype

(Phalke et al., 2009)

involved_in positive regulation of defense response to virus by host

inferred from mutant phenotype

(Durdevic et al., 2013)

involved_in positive regulation of innate immune response

inferred from mutant phenotype

(Durdevic et al., 2013)

involved_in regulation of tRNA stability

inferred from mutant phenotype

(Genenncher et al., 2018)

involved_in response to heat

inferred from mutant phenotype

(Genenncher et al., 2018, Schaefer et al., 2010)

involved_in response to oxidative stress

inferred from mutant phenotype

(Schaefer et al., 2010)

involved_in subtelomeric heterochromatin assembly

inferred from mutant phenotype

(Phalke et al., 2009)

involved_in tRNA methylation

inferred from mutant phenotype

(Genenncher et al., 2018, Schaefer et al., 2010, Goll et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasmic stress granule

inferred from direct assay

(Schaefer et al., 2010)

colocalizes_with nuclear matrix

inferred from direct assay

(Schaefer et al., 2008)

located_in nucleus

inferred from direct assay

(Kunert et al., 2003)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

DNA METHYLTRANSFERASES

TRNA METHYLTRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine (2.1.1.37)

Predictions / Assertions

Summaries

Gene Snapshot

Gene Group (FlyBase)

DNA METHYLTRANSFERASES -: DNA methyltransferases catalyze the transfer of methyl group to a DNA molecule.
TRNA METHYLTRANSFERASES -: tRNA methyltransferases catalyze the transfer of methyl group from methyl donor to a nucleoside residue in an tRNA molecule.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mt2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VKB3)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mt2-RC

FBtr0110911

NM_001042890

1114

345

Mt2-RD

FBtr0342603

NM_058127

1089

345

Additional Transcript Data and Comments

Reported size (kB)

1.2 (northern blot)

(Lyko et al., 2000)

1.65 (northern blot)

(Hung et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Mt2-PC

FBpp0110211

39.4

345

6.60

NP_001036355

ABI31309

Mt2-PD

FBpp0309545

39.4

345

6.60

NP_477475

AAF53163

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

345 aa isoforms: Mt2-PC, Mt2-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

345 (aa)

(Hung et al., 1999)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mt2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mt2_1
Mt2_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Lyko et al., 2000)

embryonic stage & larval stage

organism

(Hung et al., 1999)

adult stage | female

(Lyko et al., 2000)

ovary

(Lyko et al., 2000)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Schaefer et al., 2010)

Additional Descriptive Data

The spliced transcript isoform of Mt2 represents the major species. A minor unspliced transcript isoform is also detected by RT-PCR, particularly in testes.

(Schaefer et al., 2010)

Mt2 transcripts are observed in embryos and adult females. The female transcripts are retricted to the ovaries.

(Lyko et al., 2000)

Mt2 transcripts are most abundant in larvae, are expressed weakly in embryos, and are not detected in adults.

(Hung et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

co-fractionation

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

• cytoplasm | intense

(Schaefer et al., 2008)

organism

• nucleus

(Schaefer et al., 2008)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Schaefer et al., 2010)

embryonic stage 1 -- third instar larval stage

organism

(Schaefer et al., 2008)

adult stage | female

ovary

(Schaefer et al., 2008)

adult stage | male

testis

(Schaefer et al., 2008)

Additional Descriptive Data

Mt2 protein is enriched in cytoplasmic fractions, but purified nuclei contain significant amounts as well. Nuclear Mt2 protein is present in the insoluble nuclear matrix fraction.

(Schaefer et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasmic stress granule

inferred from direct assay

(Schaefer et al., 2010)

colocalizes_with nuclear matrix

inferred from direct assay

(Schaefer et al., 2008)

located_in nucleus

inferred from direct assay

(Kunert et al., 2003)

Expression Deduced from Reporters

Reporter: P{Mt2.genTG.EGFP}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 17

organism | ubiquitous

(Schaefer et al., 2008)

embryonic stage 2 -- 6

cell

• cytoplasm | intense

Comment: interphase

(Schaefer et al., 2008)

cell

• chromatin

Comment: mitosis

(Schaefer et al., 2008)

third instar larval stage

embryonic/larval salivary gland

(Schaefer et al., 2008)

adult stage | female

ovary

• cytoplasm | intense

(Schaefer et al., 2008)

ovary

• nucleus

(Schaefer et al., 2008)

adult stage | male

testis

• cytoplasm | intense

Comment: NOT hub cell of testis, NOT spermatozoon

(Schaefer et al., 2008)

testis

• nucleus

Comment: NOT hub cell of testis, NOT spermatozoon

(Schaefer et al., 2008)

adult stage | female & oogenesis

ovary

• cytoplasm

(Schaefer et al., 2010)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mt2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mt2

View exonic expression by tissue for Dmel\Mt2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34632/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028707

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028707

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028707

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10692

Flygut - An atlas of the Drosophila adult midgut

FBgn0028707

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mt2

Transgenic constructs containing regulatory region of Mt2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel6032

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mt2^GD5033

viable, with Scer\GAL4^Mef2.PR

Mt2^GD5033

viable, with Scer\GAL4^pnr-MD237

Mt2^GD5033

viable, with Scer\GAL4^tin.CΔ4

Mt2^GD5033

viable | recessive

Mt2^r2

Sterility

Allele

fertile

fertile | recessive

Mt2^r2

Other Phenotypes

Allele

abnormal heat stress response

Mt2⁹⁹

abnormal immune response

abnormal immune response | adult stage, with Scer\GAL4^c564

Mt2^GD5033

abnormal stress response

Mt2^GS12412

chemical resistant, with Scer\GAL4^da.G32

Mt2^UAS.cTa

chemical sensitive

Mt2⁹⁹

long lived | temperature conditional

Mt2^UAS.cTa

melanotic mass phenotype | conditional

Mt2¹⁴⁹

non-suppressor of variegation, with In(1)w^m4

Mt2¹⁴⁹

non-suppressor of variegation, with P{RS3}pan^CB-5204-3

Mt2¹⁴⁹

short lived | temperature conditional

Mt2^GS12412

suppressor of variegation | dominant, with P{RS5}5-HA-1902

Mt2¹⁴⁹

suppressor of variegation | dominant, with P{RS5}5-HA-1925

Mt2¹⁴⁹

suppressor of variegation | dominant, with P{RS5}5-HA-1992

Mt2¹⁴⁹

suppressor of variegation | dominant, with T(2;3)V21-P{lacW}92E.x3

Mt2¹⁴⁹

visible | adult stage, with Scer\GAL4^en.PU

Mt2^GD5033

Phenotype manifest in

Allele

eye disc posterior to the morphogenetic furrow, with Scer\GAL4^lz-gal4

Mt2^dsRNA.UAS

wing, with Scer\GAL4^en.PU

Mt2^GD5033

wing posterior compartment, with Scer\GAL4^en.PU

Mt2^GD5033

wing vein, with Scer\GAL4^en.PU

Mt2^GD5033

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TRDMT1

13 of 15

Yes

Hsap\DNMT1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Trdmt1

11 of 15

Yes

Mmus\Dnmt1

1 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Trdmt1

12 of 13

Yes

Rnor\Dnmt1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\trdmt1

12 of 12

Yes

Xtro\dnmt1

1 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\trdmt1

13 of 15

Yes

Drer\dnmt1

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (1)

Atha\DNMT2

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pmt1

10 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TRDMT1; tRNA aspartic acid methyltransferase 1

13 of 15

602478

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mt2

anti bait coimmunoprecipitation, western blot

(Gou et al., 2010)

Mt2 - Su(var)205

anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Gou et al., 2010)

RNA-protein

Physical Interaction

Assay

References

Mt2 - tRNA:Gly-GCC-1-1

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-10

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-11

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-12

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-13

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-2

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-3

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-4

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-5

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-6

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-7

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-8

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-1-9

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Mt2 - tRNA:Gly-GCC-2-1

anti tag coimmunoprecipitation, cross-linking study, quantitative reverse transcription pcr

(Genenncher et al., 2018)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Mt2

enhanceable

htt

(Dietz et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0028707

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-46

Cytogenetic map

33C4-33C4

Sequence location

2L:12,096,525..12,097,687 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33C4-33C4

Limits computationally determined from genome sequence between P{EP}JhI-21^EP1187 and P{lacW}Aats-thr^k04203

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

33C-33C

(Hung, 1999.9.7)

Experimentally Determined Recombination Data

Location

2-46

(FlyBase, 2016)

2-39.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27941

y¹ w^*; P{EP}Mt2^G3429

91907

y¹ sc^* v¹ sev²¹; P{TKO.GS01059}attP40

38224

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01667}attP40

42906

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02599}attP40

Kyoto

204069

y¹ w^67c23; P{GSV6}Mt2^GS12412/SM1

VDRC

v37816

w¹¹¹⁸; P{GD5033}v37816

v37815

w¹¹¹⁸; P{GD5033}v37815/TM3

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GM14972

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028707

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028707

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34632

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gou et al., 2010, Schaefer et al., 2008)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Dnmt Mt2

(FlyBase, 1996-)

Additional comments

Source for merge of Dnmt Mt2 was sequence comparison ( date:010313 ).

(FlyBase, 1996-)

Other Comments

Overexpression of Dnmt2 results in an increase in genomic DNA methylation.

(Kunert et al., 2003)

Several aspects of the organisation of the divergently transcribed, coordinately expressed Dnmt2 and CG6712 open reading frames suggest that they are components of a transposon-like element (although each of the two open reading frames appears to be encoded by a single copy locus in the genome at 33C--33D). Dnmt2 and CG6712 are tightly linked and neither open reading frame is interrupted by introns. There is a 12bp inverted repeat close to the 3' ends of both the open reading frames. Sequences homologous to Dnmt2 are present in a limited distribution of Drosophila species. Overall orientation not stated: Dnmt2- CG6712+

(Lyko et al., 2000)

Identification: on basis of sequence similarity to mammalian dnmt2 and yeast pmt1.

(Hung et al., 1999)

Potentially encodes a cytosine DNA methyltransferase although bacterially expressed Dnmt2 is catalytically inactive in vitro.

(Tweedie et al., 1999)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34632

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VKB3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VKB3

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028707

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34632/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028707

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mt2_1
Mt2_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028707

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028707

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10692

Flygut - An atlas of the Drosophila adult midgut

FBgn0028707

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34632

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005511

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10692

MARRVEL_MODEL - MARRVEL (model organism gene)

34632

modMine - A data warehouse for the modENCODE project

FBgn0028707

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0028707

DRSC - Results frm RNAi screens

FBgn0028707

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028707

FlyMine - An integrated database for Drosophila genomics

FBgn0028707

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG10692

(Kumar et al., 2016, Parkhitko et al., 2016, Ni et al., 2010.12.1)

DNMT

(Ismail et al., 2020, Panikar et al., 2015, Hendrich and Tweedie, 2003)

DNMT2

(Bhattacharya et al., 2021, Deobagkar, 2018, Deshmukh et al., 2018, Heil, 2014, van Steensel et al., 2010, Taylor-Harding et al., 2004)

DmMT2

(Hung et al., 1999)

Dnmt

Mt2

(Yamaguchi et al., 2021, Abhyankar et al., 2018, Bhattacharya et al., 2017, Transgenic RNAi Project members, 2017-, Dietz et al., 2015, Takayama et al., 2014, Dunwell et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Pezzulo et al., 2012, Doheny et al., 2008, Alfonso and Maggert, 2007, Brown and Feder, 2005, Gorr et al., 2004, Kunert et al., 2003, FlyBase, 1996-)

dDNMT

(Tweedie et al., 1999)

dDNMT2

(Salzberg et al., 2004)

dDnmt2

(Yang et al., 2020, Dietz et al., 2015, Morrow and Tanguay, 2015, Garcia et al., 2007, Goll et al., 2006, Lin et al., 2005, Kunert et al., 2003, Reddy et al., 2003, Tang et al., 2003, Lyko, 2002, Lyko, 2001, Lyko et al., 2000)

dnmt2

(Onorati et al., 2015, Yadlapalli and Yamashita, 2013, Yamashita, 2013, Hung, 1999.9.7)

nmt2

Name Synonyms

DNA methyltransferase 2

(Gou et al., 2010)

DNA(5-cytosine) methyltransferase

Methyltransferase 2

cytosine-5 methyltransferase

(Schaefer et al., 2009)

Secondary FlyBase IDs

FBgn0029313

Datasets (10)

Study focus (2)

Experimental Role

Project

Project Type

Title

allele_used

modENCODE_chromatin_proteins_flies

genome binding

Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.

allele_used

modENCODE_chromatin_histones_flies

genome binding

Genome-wide localization of histones and their modifications in fly tissues by ChIP-chip and ChIP-Seq.

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (95)

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