protein is distributed granularly in the cytoplasm of embryonic muscles, and is accumulated in the muscle tip, where the muscle attaches to the tendon cell.
protein expression is ubiquitous within neurons and relatively uniform between neurons throughout adult brain
protein is cytoplasmic and present in discrete granules. Upon heat shock, Fmr1
protein concentrates in large cytoplasmic granules.
protein is expressed in the larval and adult testis. Expression is modest in spermatogonia, strong in spermatocytes, and undetectable in terminal epithelial cells of testis. Fmr1
protein is present at a low level in elongated spermatids, but absent in late-stage elongated spermatids.
protein expression is enriched in the brain. It is expressed specifically in neurons and is excluded from glia. In pupal and adult brains, it is expressed in most, if not all neurons.
The 85 kD band is detectable via Western blot at all embryonic stages and in S2 cells. THe 82 kD band is observed in S2 cells and in embryos colleced 9-12 hours AEL. Embryonic expression of Fmr1
protein is first detectable in the somatic musculature at embryonic stage 9. By stage 12, Fmr1
protein accumulates in the brain and ventral nerve cord. In late embryos high levels of expression are observed in the pharyngeal muscle, gonads, visceral muscle and hindgut. Expression is also observed in the tips of scolopidial dendrites in thoracic and abdominal segments, and at muscle attachment sites. In first instar larvae, the expression in scolopidial neurons extend to the entire scolopidial dendrite. In third instar larvae, expression persists in muscles, but is primarily localized to the central nervous system. Fmr1
protein is heavily expressed in the larval mushroom body, and accumulates in the cell bodies, dendrites, and axons of Kenyon cells. Expression is widespread in the ventral nerve cord, where it is cytoplasmically localized. In the larval testis, expression is localized cytoplasmically in spermatocytes, but is excluded from spermatogonia. In the eye disc, Fmr1
protein is observed at the morphogenetic furrow, in the axon of the Bolwig nerve, and in maturing ommatidia. Ommatidial expression appears to be restricted to photoreceptor cell R8.