FB2020_06, released Dec 22, 2020

Gene: Dmel\GMF

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General Information
Symbol
Dmel\GMF
Species
D. melanogaster
Name
Glia maturation factor
Annotation Symbol
CG5869
Feature Type
protein_coding_gene
FlyBase ID
FBgn0028894
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Snapshot
Glia maturation factor (GMF) encodes a protein belonging to the ADF-H domain family of proteins, which promote dissambly of actin networks. It specifically binds to and promotes disassembly of Arp2/3-nucleated lamellipodial actin networks. It plays a role in promoting protrusion dynamics during cell migration. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

BG:DS02740.9 , DS02740.9

Key Links
Genomic Location
Cytogenetic map
35F1-35F1
Sequence location
2L:16,301,656..16,302,954 [-]
Recombination map

2-52

RefSeq locus
NT_033779 REGION:16301656..16302954
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables actin binding
inferred from electronic annotation with InterPro:IPR002108
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables Arp2/3 complex binding
inferred from biological aspect of ancestor with PANTHER:PTN000882413
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in actin filament debranching
inferred from direct assay
(Poukkula et al., 2014)
involved_in border follicle cell migration
inferred from mutant phenotype
(Poukkula et al., 2014)
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation
inferred from direct assay
(Poukkula et al., 2014)
inferred from genetic interaction with FLYBASE:Arpc5; FB:FBgn0031437, FLYBASE:flr; FB:FBgn0260049
(Poukkula et al., 2014)
involved_in regulation of actin filament polymerization
inferred from mutant phenotype
(Poukkula et al., 2014)
involved_in regulation of cell projection organization
inferred from mutant phenotype
(Poukkula et al., 2014)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in actin filament debranching
inferred from biological aspect of ancestor with PANTHER:PTN000882413
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation
inferred from biological aspect of ancestor with PANTHER:PTN000882413
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cell periphery
inferred from direct assay
(Poukkula et al., 2014)
located_in lamellipodium
inferred from direct assay
(Poukkula et al., 2014)
located_in nucleus
inferred from direct assay
(Poukkula et al., 2014)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Poukkula et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\GMF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
GMF-RA
FBtr0080851
NM_135943
1019
138
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
GMF-PA
FBpp0080408
16.5
138
4.64
NP_609787
AAF53517
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GMF using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis
border follicle cell
(Poukkula et al., 2014)
polar follicle cell
(Poukkula et al., 2014)
centripetal follicle cell
(Poukkula et al., 2014)
Additional Descriptive Data

GMF protein dGMF is expressed in the follicular epithelium

(Poukkula et al., 2014)

and is more prominent in polar cells, migrating border cells, and centripedal cells.

(Poukkula et al., 2014)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cell periphery
inferred from direct assay
(Poukkula et al., 2014)
located_in lamellipodium
inferred from direct assay
(Poukkula et al., 2014)
located_in nucleus
inferred from direct assay
(Poukkula et al., 2014)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Poukkula et al., 2014)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\GMF in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of GMF
Transgenic constructs containing regulatory region of GMF
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Phenotype manifest in
Allele
border follicle cell
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\GMFB
13 of 15
Yes
Yes
Hsap\GMFG
13 of 15
Yes
Yes
Hsap\CD19
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Gmfb
14 of 15
Yes
Yes
Mmus\Gmfg
12 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Gmfg
12 of 13
Yes
Yes
Rnor\Gmfb
10 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\gmfb
9 of 12
Yes
Yes
Xtro\gmfg
4 of 12
No
Yes
Danio rerio (Zebrafish) (2)
Drer\gmfb
13 of 15
Yes
Yes
Drer\gmfg
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\Y50D7A.10
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\ADF1
1 of 9
Yes
No
Atha\ADF4
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\AIM7
9 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\gmf1
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190I56 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001753
Drosophila simulans
Dsim\GD21923
Drosophila sechellia
Dsec\GM17913
Drosophila erecta
Dere\GG24175
Drosophila yakuba
Dyak\GE19369
Drosophila ananassae
Dana\GF15327
Drosophila pseudoobscura pseudoobscura
Dpse\GA19191
Drosophila persimilis
Dper\GL14094
Drosophila willistoni
Dwil\GK24743
Drosophila virilis
Dvir\GJ17165
Drosophila mojavensis
Dmoj\GI14715
Drosophila grimshawi
Dgri\GH11432
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150FGK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015154
Glossina morsitans
Tsetse fly
Gmor\GMOY003078
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10170
Mayetiola destructor
Hessian fly
Mdes\Mdes015823
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007334
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013768
Anopheles gambiae
Malaria mosquito
Agam\AGAP009059
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ007823
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0JAX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA011372
Danaus plexippus
Monarch butterfly
Dple\DPOGS214393-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL008226
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010634
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08996
Tribolium castaneum
Red flour beetle
Tcas\TC014337
Pediculus humanus
Human body louse
Phum\PHUM348160
Rhodnius prolixus
Kissing bug
Rpro\RPRC014580
Cimex lectularius
Bed bug
Clec\CLEC011449
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005516
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0JFB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR005689
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_324231
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0YYO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Gmfb
Gallus gallus
Domestic chicken
Ggal\GMFB
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    GMFB; glia maturation factor beta
    13 of 15
    601713
        GMFG; glia maturation factor gamma
        13 of 15
        604104
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Interactions Browser
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            flr
            enhanceable
            GMF
            (Poukkula et al., 2014)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2L
            Recombination map

            2-52

            Cytogenetic map
            35F1-35F1
            Sequence location
            2L:16,301,656..16,302,954 [-]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            35F1-35F1
            Limits computationally determined from genome sequence between P{lacW}heixk11403&P{lacW}heixk12401 and P{EP}fzyEP1028&P{lacW}mRpL4k14608
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            2-52

            (FlyBase, 2016)

            2-49.7

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (3)
            Genomic Clones (12)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (40)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Poukkula et al., 2014)
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of

              Source for identity of: GMF CG5869

              (Poukkula et al., 2014)
              Source for database merge of

              Source for merge of: BG:DS02740.9 BcDNA:SD03793

              (FlyBase, 1996-)
              Additional comments

              Source for merge of BG:DS02740.9 BcDNA:SD03793 was a shared cDNA ( date:020730 ).

              (FlyBase, 1996-)
              Other Comments
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 32 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              FlyMine - An integrated database for Drosophila genomics
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (9)
              Reported As
              Symbol Synonym
              BG:DS02740.9
              (Giot et al., 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)
              BcDNA:SD03793
              CG5869
              (Poukkula et al., 2014, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)
              DS02740.9
              (Reese et al., 2000, Reese et al., 2000)
              GMF
              (Hall et al., 2019, Poukkula et al., 2014)
              dGMF
              (Poukkula et al., 2014)
              gmf
              (Poukkula et al., 2014)
              Name Synonyms
              Glia maturation factor
              glia-maturation factor
              (Poukkula et al., 2014)
              Secondary FlyBase IDs
              • FBgn0062894
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (34)