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Gene Dmel\trbl

General Information
Symbol	Dmel\trbl	Species	D. melanogaster
Name	tribbles	Annotation symbol	CG5408
Feature type	protein_coding_gene	FlyBase ID	FBgn0028978
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	trb
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	77C1-77C1	Sequence location	3L:20,389,388..20,394,709 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene tribbles is referred to in FlyBase by the symbol Dmel\trbl (CG5408, FBgn0028978). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: ventral furrow formation; negative regulation of mitosis; regulation of cell cycle. 35 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; organ system; portion of tissue; sense organ; anatomical structure; eo support cell; external compound sense organ; organ system subdivision; adult segment; cell cycle; epithelial furrow. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein kinase, catalytic domain; Protein kinase-like domain; Pseudokinase tribbles family/serine-threonine-protein kinase 40. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, larval/adult hindgut, adult Malpighian tubules, adult fat body, adult female reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1629044_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of trbl. Gene sequence location is 3L:20389388..20394709.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 77C1-77C1   Limits computationally determined from genome sequence between P{PZ}eRF100103 and P{EP}trblEP3519
Experimentally Determined Cytological Location
Cytogenetic map Notes References 77C1-77C2   (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000) 77C-77C     (Seher, 1999.11.15)
Experimentally Determined Recombination Data
Location	3- (Grosshans and Wieschaus, 2000)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\trbl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) trbl-RA FBtr0078235  NM_079933   2372   484
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein trbl-PA   FBpp0077893   54.1   484   5.13     NP_524672   AAF51590
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Pseudokinase tribbles family/serine-threonine-protein kinase 40 (IPR024104)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698927   AE014296 AAF51590   AF204688 AAF26374   AI258668   AI387902   AI387940   AI404427   AI531024   AI532690   AQ025182   AQ025311   BF488529   BF500967   BF501057   BH614863   BI141944   BI163166   BI163177   BI173849   BI362262   BI365680   BI482525   BI565528   BI570773   BI571700   BI581880   BI581890   BI588598   BI591733   BI592873   BI627215   BI627479   BI627841   BT004834 AAO45190   CK134886   CK657043   CO177284   CO303728   CO318438   CO320099   CO320794   CO332625   CO333607   CZ473233   CZ473259   CZ475911   CZ476668   EC051018   EC052003   EC053046   EC055444   EC055447   EC067951   EC081040   EC087704   EC090130   EC204332   EC206114   EC227541   EC227548   EC246853   ES691089   GH749787   GH751582   GH753099   GH754592   GH759315   GH759754   GH761024   GH770919   GH771146   GH772003   GH772450   GH772829   GH772943   GH773164   GH774235   GH775781   GH775834   GH777509   GH783302   GH785005   GH785475   GH786979   GH789023   GH790264   GH791962   GH794230   GH794231   GH795124   GH799479   GH802706   GH802807   GH803056   GH806080   GH806260   GH810513   GH810572   GH815068   GH815492   GH816059   GH816231   GH817015   GH817111   GH818496   GH820283   GH820426   GH820881   GH822067   GH823447   GH824549   GH825068   GH825303   GH826926   GH830333   GH835856   GH836593   GH840086   GH840348   GH840698   GH841214   GH842219   GH844432   GH845615   GH845743   GH848193   GH848206   GH851096   GH853476   GH854761   GH854765   GH857581   GH857934   GH858606   GH858642   GH859704   GH860900   GH861891   GH864782   GH866089   GH868061   GH869546   GH870585   GH871147   GH872728   GH873477   GH873677   GH875047   GH875300   GH875669   GH877917   GH881823   GH883349   GH886532   GH886672   GH886838   GH886958   GH887159   GH887181   GH887485   GH890209   GH890469   GH892205   GH896687   GH896989   GH897552   GH897574   GH898201   GH900055   GH900546   GH904816   GH905115   GH909856   GH912930   GH914234   GH916126   GH916417   GH917546   GH921422   GH921989   GH922409   GH922625   GH922744   GH925158   GH928268   GH929059   GH933790   GH933833   GH934020   GH934414   GH938834   GH939323   GH940730   GH942395
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V3Z1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000436119 3L:20,394,271..20,396,658 evidence=Reporter construct (in vivo) (Sandmann et al., 2007, Halfon, 2012.6.19) regulatory region FBsf0000436385 3L:20,395,366..20,396,365 evidence=Reporter construct (in vivo) (Rouault et al., 2010, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   anlage in statu nascendi   (Fisher et al., 2012) organism | segmentally repeated   (Fisher et al., 2012) organism | intense & ubiquitous   (Fisher et al., 2012) embryonic stage 5   organism | ventral   (Mata et al., 2000) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal ectoderm   (Fisher et al., 2012) organism | intense & ubiquitous   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal ectoderm   (Fisher et al., 2012) organism | intense & ubiquitous   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic optic lobe primordium   (Fisher et al., 2012) midline primordium   (Fisher et al., 2012) pars intercerebralis primordium   (Fisher et al., 2012) stomatogastric nervous system primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic central brain pars intercerebralis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic optic lobe   (Fisher et al., 2012) embryonic stomatogastric nervous system   (Fisher et al., 2012) embryonic/larval dorsal vessel   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral midline   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Mata et al., 2000)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, larval/adult hindgut, adult Malpighian tubules, adult fat body, adult female reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    64.325 Larval Midgut    88.4 Larval Hindgut    130.5 Larval Malpighian Tubules    58.5 Larval Fat Body    41.1 Larval Salivary Gland    92.3 Larval Trachea    59.625 Larval Carcass    73.05 Adult Head    40 Adult Eye    34.475 Adult Brain    15.9 Adult Thoracic-Abdominal Ganglion    17.7 Adult Crop    88.2 Adult Midgut    119.3 Adult Hindgut    151.8 Adult Malpighian Tubules    174.2 Adult Fat Body    119.5 Adult Salivary Gland    62.1 Adult Heart    53.1 Adult VirginFemale Spermatheca    352.8 Adult InseminatedFemale Spermatheca    118.7 Adult Ovary    101.7 Adult Testis    37.2 Adult Male Accessory Gland    37.6 Adult Carcass    98.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    40   NA  Eye    34.475   NA  Brain    no informative data   64.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    88.2   no informative data  Midgut    119.3   130.5  Hindgut    151.8   58.5  Malpighian Tubules    174.2   no informative data  Fat Body    119.5   92.3  Salivary Gland    no informative data   NA  Heart    53.1   59.625  Trachea    NA   NA  VirginFemale Spermatheca    352.8   NA  InseminatedFemale Spermatheca    118.7   NA  Ovary    101.7   NA  Testis    37.2   NA  Male Accessory Gland    no informative data   73.05  Carcass    98.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (31) central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering  (29) digestive system, 1-day adult  (28) digestive system, 4-day adult    24 digestive system, 20-day adult  (38) fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering  26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult  (28) ovary, virgin 4-day female  (43) ovary, mated 4-day female  (51) testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering  29 digestive system, 1-day adult  28 digestive system, 4-day adult    24 digestive system, 20-day adult  (38) fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering  26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult  28 ovary, virgin 4-day female  (43) ovary, mated 4-day female  (51) testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    15 central nervous system, pupae P8    5 head, virgin 1-day female    9 head, virgin 4-day female    18 head, virgin 20-day female    19 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    17 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    28 digestive system, 4-day adult    24 digestive system, 20-day adult    38 fat body, larvae L3 wandering    9 fat body, white prepupae    7 fat body, pupae P8    17 carcass, larvae L3 wandering    26 carcass, 1-day adult    17 carcass, 4-day adult    17 carcass, 20-day adult    28 ovary, virgin 4-day female    43 ovary, mated 4-day female    51 testis, mated 4-day male    15 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    161 embryo 04-06hr    129 embryo 06-08hr    122 embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (31) embryo 02-04hr  (161) embryo 04-06hr  (129) embryo 06-08hr  (122) embryo 08-10hr  (46) embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr  (29) embryo 16-18hr  (75) embryo 18-20hr  (92) embryo 20-22hr  (31) embryo 22-24hr  (34) larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr  (34) white prepupae 24hr  26 pupae 2d postWPP  (38) pupae 3d postWPP    18 pupae 4d postWPP  (45) adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day  (30) adult female 05day    25 adult female 30day  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr  (161) embryo 04-06hr  (129) embryo 06-08hr  (122) embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    161 embryo 04-06hr    129 embryo 06-08hr    122 embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    161 embryo 04-06hr    129 embryo 06-08hr    122 embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  31 embryo 02-04hr  (161) embryo 04-06hr  (129) embryo 06-08hr  (122) embryo 08-10hr  (46) embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr  29 embryo 16-18hr  (75) embryo 18-20hr  (92) embryo 20-22hr  31 embryo 22-24hr  (34) larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr  (34) white prepupae 24hr  26 pupae 2d postWPP  (38) pupae 3d postWPP    18 pupae 4d postWPP  (45) adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day  30 adult female 05day    25 adult female 30day  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr  (161) embryo 04-06hr  129 embryo 06-08hr  122 embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    161 embryo 04-06hr    129 embryo 06-08hr    122 embryo 08-10hr    46 embryo 10-12hr    21 embryo 12-14hr    16 embryo 14-16hr    29 embryo 16-18hr    75 embryo 18-20hr    92 embryo 20-22hr    31 embryo 22-24hr    34 larva L1    15 larva L2    14 larva L3 12hr old    19 larva L3 puffstage 1-2    15 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    16 white prepupae new    18 white prepupae 12hr    34 white prepupae 24hr    26 pupae 2d postWPP    38 pupae 3d postWPP    18 pupae 4d postWPP    45 adult male 01day    17 adult male 05day    17 adult male 30day    21 adult female 01day    30 adult female 05day    25 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1    126 wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9    121 wing disc ML-DmD16-c3    76 wing disc ML-DmD21    162 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    121 CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2    109 tumorous blood cells mbn2    28 ovary fGS/OSS    155 ovary OSC    53 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  (73) embryonic 1182-4H  (67) embryonic GM2  (93) embryonic Kc167  (89) embryonic S1  (36) embryonic S3  (74) leg disc CME L1  (126) wing disc CME-W2  (68) wing disc ML-DmD8  (95) wing disc ML-DmD9  (121) wing disc ML-DmD16-c3  (76) wing disc ML-DmD21  (162) wing disc ML-DmD32  (47) haltere disc ML-DmD17-c3  (35) eye-antennal disc ML-DmD11  (60) antennal disc ML-DmD20-c5  (79) mixed discs ML-DmD4-c1  (121) CNS ML-DmBG1-c1  (77) CNS ML-DmBG2-c2  (109) tumorous blood cells mbn2  (28) ovary fGS/OSS  (155) ovary OSC  (53) ovary OSS  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1  (126) wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9  (121) wing disc ML-DmD16-c3    76 wing disc ML-DmD21  (162) wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1  (121) CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2  (109) tumorous blood cells mbn2    28 ovary fGS/OSS  (155) ovary OSC    53 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1    126 wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9    121 wing disc ML-DmD16-c3    76 wing disc ML-DmD21    162 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    121 CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2    109 tumorous blood cells mbn2    28 ovary fGS/OSS    155 ovary OSC    53 ovary OSS    40 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1    126 wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9    121 wing disc ML-DmD16-c3    76 wing disc ML-DmD21    162 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    121 CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2    109 tumorous blood cells mbn2    28 ovary fGS/OSS    155 ovary OSC    53 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  (73) embryonic 1182-4H  (67) embryonic GM2  (93) embryonic Kc167  (89) embryonic S1  (36) embryonic S3  (74) leg disc CME L1  (126) wing disc CME-W2  (68) wing disc ML-DmD8  (95) wing disc ML-DmD9  (121) wing disc ML-DmD16-c3  (76) wing disc ML-DmD21  (162) wing disc ML-DmD32  (47) haltere disc ML-DmD17-c3  (35) eye-antennal disc ML-DmD11  (60) antennal disc ML-DmD20-c5  (79) mixed discs ML-DmD4-c1  (121) CNS ML-DmBG1-c1  (77) CNS ML-DmBG2-c2  (109) tumorous blood cells mbn2  28 ovary fGS/OSS  (155) ovary OSC  (53) ovary OSS  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1  126 wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9  121 wing disc ML-DmD16-c3    76 wing disc ML-DmD21  (162) wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1  121 CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2  109 tumorous blood cells mbn2    28 ovary fGS/OSS  (155) ovary OSC    53 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    73 embryonic 1182-4H    67 embryonic GM2    93 embryonic Kc167    89 embryonic S1    36 embryonic S3    74 leg disc CME L1    126 wing disc CME-W2    68 wing disc ML-DmD8    95 wing disc ML-DmD9    121 wing disc ML-DmD16-c3    76 wing disc ML-DmD21    162 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    35 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    121 CNS ML-DmBG1-c1    77 CNS ML-DmBG2-c2    109 tumorous blood cells mbn2    28 ovary fGS/OSS    155 ovary OSC    53 ovary OSS    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult    141 Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    112 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (38) cold shock, 4-day adult  26 heat shock, 4-day adult  (49) Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult  (45) Cadmium 100 mM 48 hrs, 4-day adult  (141) Copper 0.5 mM 12 hrs, larvae L3  (31) Copper 15 mM 48 hrs, 4-day adult  (38) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  (27) Ethanol 2.5% 3 hrs, larvae L3  (112) Ethanol 5% 3 hrs, larvae L3  (34) Ethanol 10% 3 hrs, larvae L3  (27) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (28) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (69) Caffeine 25 mg/ml 48 hrs, 4-day adult  (32) Paraquat 5 mM 48 hrs, 4-day adult  (41) Paraquat 10 mM 48 hrs, 4-day adult  (105) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult  (141) Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3  (112) Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult  105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult    141 Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    112 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult    141 Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    112 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (38) cold shock, 4-day adult  26 heat shock, 4-day adult  (49) Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult  (45) Cadmium 100 mM 48 hrs, 4-day adult  (141) Copper 0.5 mM 12 hrs, larvae L3  31 Copper 15 mM 48 hrs, 4-day adult  (38) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  27 Ethanol 2.5% 3 hrs, larvae L3  (112) Ethanol 5% 3 hrs, larvae L3  (34) Ethanol 10% 3 hrs, larvae L3  27 Caffeine 1.5 mg/ml 4 hrs, larvae L3  28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (69) Caffeine 25 mg/ml 48 hrs, 4-day adult  32 Paraquat 5 mM 48 hrs, 4-day adult  (41) Paraquat 10 mM 48 hrs, 4-day adult  (105) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult  141 Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3  112 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult  105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    38 cold shock, 4-day adult    26 heat shock, 4-day adult    49 Cadmium 50 mM 6 hrs, larvae L3    22 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    45 Cadmium 100 mM 48 hrs, 4-day adult    141 Copper 0.5 mM 12 hrs, larvae L3    31 Copper 15 mM 48 hrs, 4-day adult    38 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    27 Ethanol 2.5% 3 hrs, larvae L3    112 Ethanol 5% 3 hrs, larvae L3    34 Ethanol 10% 3 hrs, larvae L3    27 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    105 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_31 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • RH69304 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028978 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5408-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for trbl Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | partially | recessive, with Scer\GAL4[-]: trblEP1119 partially lethal - majority die trblCB02744 partially lethal - majority die | polyphasic trblunspecified partially lethal - majority live, with Scer\GAL4[-]: trblEP1119 trblEP3519 viable, with Scer\GAL4pnr-MD237 trblGD11640 Sterility Allele female sterile trblunspecified Other Phenotypes Allele decreased cell number, with Scer\GAL4bam.T:Hsim\VP16 trblScer\UAS.P\T.cMa decreased cell number, with Scer\GAL4en-e16E trblScer\UAS.cMa increased cell size, with Scer\GAL4en-e16E trblScer\UAS.cMa memory defective trbl3-54 mitotic cell cycle defective trbldsRNA.cSa mitotic cell cycle defective, with Scer\GAL4[-]: trblEP3519 mitotic cell cycle defective | recessive, with Scer\GAL4[-]: trblEP1119 visible, with Scer\GAL4Bx-MS1096 trblScer\UAS.cGa visible, with Scer\GAL4GMR.PF trblScer\UAS.cGa visible, with Scer\GAL4sca-537.4 trblEP3519 visible, with Scer\GAL4sd-SG29.1 trblScer\UAS.cGa wild-type trbl+tMa wild-type, with Scer\GAL4ap-md544 trblEP3519 wild-type, with Scer\GAL4GMR.12 trblEP3519 wild-type, with Scer\GAL4GMR.15 trblEP3519 wild-type, with Scer\GAL4hs.2sev trblEP3519 wild-type, with Scer\GAL4Kr.PM trblEP3519 wild-type, with Scer\GAL4prd.RG1 trblEP3519 wild-type, with Scer\GAL4sd-SG29.1 trblEP3519 wild-type, with Scer\GAL4twi.PG trblEP3519 Phenotype manifest in Allele border follicle cell, with Scer\GAL4slbo.2.6 trblEP3519 trblScer\UAS.cMa cystocyte, with Scer\GAL4[-]: trblEP1119 cystocyte (with Df(3L)rdgC-co2), with Scer\GAL4[-]: trblEP1119 cystocyte (with Df(3L)ri-79c), with Scer\GAL4[-]: trblEP1119 cystocyte | ectopic, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblEPg35131 trblScer\UAS.P\T.cMa egg chamber, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblEPg35131 trblScer\UAS.P\T.cMa external sensory organ precursor cell I, with Scer\GAL4neur-P72 trblScer\UAS.cMb eye, with Scer\GAL4GMR.PF trblScer\UAS.cGa gastrula embryo, with Scer\GAL4[-]: trblEP1119 germline cyst, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblEPg35131 interommatidial bristle, with Scer\GAL4GMR.PF trblScer\UAS.cGa macrochaeta trblBG00964 macrochaeta, with Scer\GAL4neur-P72 trblScer\UAS.cMb macrochaeta, with Scer\GAL4sca-537.4 trblEP3519 mesoderm anlage, with Scer\GAL4[-]: trblEP1119 mitotic cell cycle trbldsRNA.cSa mitotic cell cycle, with Scer\GAL4[-]: trblEP3519 mitotic domain 1, with Scer\GAL4[-]: trblEP1119 mitotic domain 2, with Scer\GAL4[-]: trblEP1119 mitotic domain 3, with Scer\GAL4[-]: trblEP1119 mitotic domain 10, with Scer\GAL4[-]: trblEP3519 nurse cell | ectopic, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblScer\UAS.P\T.cMa oocyte, with Scer\GAL4[-]: trblEP1119 oocyte (with Df(3L)rdgC-co2), with Scer\GAL4[-]: trblEP1119 oocyte (with Df(3L)ri-79c), with Scer\GAL4[-]: trblEP1119 oocyte | ectopic, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblScer\UAS.P\T.cMa ovary, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblScer\UAS.P\T.cMa spermatogonial cyst, with Scer\GAL4bam.T:Hsim\VP16 trblScer\UAS.P\T.cMa spermatogonium, with Scer\GAL4nos.UTR.T:Hsim\VP16 trblEP3519 trblEPg35131 tormogen cell, with Scer\GAL4neur-P72 trblScer\UAS.cMb tormogen cell, with Scer\GAL4sca-537.4 trblEP3519 trichogen cell, with Scer\GAL4neur-P72 trblScer\UAS.cMb trichogen cell, with Scer\GAL4sca-537.4 trblEP3519 ventral furrow trbldsRNA.cSa ventral furrow, with Scer\GAL4[-]: trblEP3519 wing, with Scer\GAL4Bx-MS1096 trblScer\UAS.cGa wing | posterior compartment, with Scer\GAL4en-e16E trblScer\UAS.cMa wing disc | posterior compartment, with Scer\GAL4en-e16E trblScer\UAS.cMa wing margin, with Scer\GAL4sd-SG29.1 trblScer\UAS.cGa
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of trbl Class Mutagen Stocks Known lesion trblKG02308 P-element activity 2 -- trblKG07411 2 -- trbl5-HA-1074 1 -- trbld03175 1 -- trbld03251 P-element activity 1 -- trbld07751 P-element activity 1 -- trblMI01025 1 -- trblNP3530 1 -- trbl3-54 0 Yes trblBG00964 P-element activity 0 Yes trblCB02744 P-element activity 0 Yes trbld03196 P-element activity 0 -- trbld09302 P-element activity 0 -- trblDG29201 0 -- trblEP1119 loss of function allele P-element activity 0 Yes trblEP3519 P-element activity 0 Yes trblEPg35131 P-element activity 0 Yes trblGS9628 0 Yes trblP7.9.02 Delta2-3 0 Yes trblunspecified 0 --
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of trbl Class Mutagen Stocks Known lesion trblGD11640 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes trblGL01337 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes trblHMJ02089 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes trblHMS02198 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes trblKK108667 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes trbl+tMa in vitro construct - genomic fragment 0 Yes trblcGa in vitro construct 0 Yes trbldsRNA.cSa in vitro construct - RNAi 0 -- trblK266R in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes trblMtnA.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes trblMtnA.T:Uuuu\T7 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes trblScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes trblScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes trblScer\UAS.cMb in vitro construct - regulatory fusion 0 Yes trblScer\UAS.P\T.cMa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6136 (Ryder, 2004.4.26) Df(3L)rdgC-co2 (Seher, 2000.3.3, Grosshans and Wieschaus, 2000, Mata et al., 2000) Df(3L)ri-79c (Seher, 2000.3.3, Grosshans and Wieschaus, 2000, Mata et al., 2000)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11640} NA P{KK108667} NA P{TRiP.GL01337} NA P{TRiP.HMJ02089} NA P{TRiP.HMS02198} NA P{UASp-trbl.M} NA P{UAS-trbl.G} NA P{UAS-trbl.M} NA P{UAS-trbl.Mb} NA characterization construct P{trbl+tMa} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}trblEP1119 NA P{EP}trblEP3519 NA P{EPg}trblEPg35131 NA P{GSV6}trblGS9628 NA P{Mae-UAS.6.11}trblP7.9.02 NA P{XP}d03175 NA P{XP}trbld03196 NA P{XP}trbld03251 NA P{XP}trbld07751 NA P{XP}trbld09302 NA miscellaneous insertions Mi{MIC}trblMI01025 NA P{Ecol\lacO.4x.w}6.4P5 NA P{GT1}trblBG00964 NA P{PTT-GB}trblCB02744 NA P{RS5}trbl5-HA-1074 NA P{SUPor-P}trblKG02308 NA P{SUPor-P}trblKG07411 NA P{wHy}trblDG29201 NA insertion of enhancer trap binary system P{GawB}trbl3-54 No P{GawB}trblNP3530 No insertion of enhancer trap P{lacW}W12W12 No
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
negative regulation of mitosis inferred from genetic interaction with stg AND inferred from mutant phenotype (Grosshans and Wieschaus, 2000) regulation of cell cycle inferred from mutant phenotype (Mata et al., 2000) ventral furrow formation inferred from genetic interaction with stg (Grosshans and Wieschaus, 2000)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-) protein kinase activity inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-) protein serine/threonine kinase activity inferred from sequence or structural similarity (Seher and Leptin, 2000) NOT protein serine/threonine kinase activity inferred from sequence or structural similarity (Grosshans and Wieschaus, 2001) traceable author statement (Lee and Orr-Weaver, 2003)
Biological Process
CV term Evidence References
gastrulation non-traceable author statement (Lee and Orr-Weaver, 2003) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
trbl - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Arpc3A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Arpc4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG13321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG13472 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG16789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG9231 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CG9911 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Cbp80 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - CtBP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Pdi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - Wbp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - mRpL52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - mRpS7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) trbl - pic anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions trbl allele Gene References trblEP1119 slbo (Rorth et al., 2000) stg (Mata et al., 2000) trblEP3519 H (Abdelilah-Seyfried et al., 2000) N (Abdelilah-Seyfried et al., 2000) slbo (Rorth et al., 2000) trblEPg35131 stg (Mata et al., 2000) trblScer\UAS.cGa wee (Price et al., 2002) trblScer\UAS.cMa stg (Mata et al., 2000) unspecified H (Mueller et al., 2005) os (Mukherjee et al., 2006) pbl (Gregory et al., 2007) W (Mueller et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68764 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028978 DroID - A comprehensive database of gene and protein interactions. • FBgn0028978 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43999 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9V3Z1
Orthologs
OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6G4H98 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6V6XDD Arthropod inclusive ortholog search EOG6XKSQR Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6G4H98)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\trbl     Drosophila simulans   Dsim\GD12194 Y   Drosophila sechellia   Dsec\GM22222 Y   Drosophila erecta   Dere\GG13319 Y   Drosophila yakuba   Dyak\GE22407 Y   Drosophila yakuba   Dyak\GE22748     Drosophila ananassae   Dana\GF24140 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18860 Y   Drosophila persimilis   Dper\GL11911 Y   Drosophila willistoni   Dwil\GK17227 Y   Drosophila virilis   Dvir\GJ11840 Y   Drosophila mojavensis   Dmoj\GI13476 Y   Drosophila grimshawi   Dgri\GH16753 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6V6XDD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54949   Apis mellifera Western honey bee  Amel\GB54947   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001208   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11507   Atta cephalotes Leafcutter ant  Acep\ACEP23858   Atta cephalotes Leafcutter ant  Acep\ACEP23860   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21167   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13858   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13859   Linepithema humile Argentine ant  Lhum\LH25161   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23940   Solenopsis invicta Red fire ant  Sinv\SINV16239   Solenopsis invicta Red fire ant  Sinv\SINV16240   Solenopsis invicta Red fire ant  Sinv\SINV16246   Bombyx mori Silkmoth  Bmor\BGIBMGA006138   Pediculus humanus Human body louse  Phum\PHUM463670   Pediculus humanus Human body louse  Phum\PHUM463550   Tribolium castaneum Red flour beetle  Tcas\TC013694
Orthologs in non-Insect Arthropods (EOG6XKSQR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Snap25   Ixodes scapularis Deer tick  Isca\ISCW016855
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12194 Drosophila sechellia Dsec\GM22222 Drosophila erecta Dere\GG13319 Drosophila yakuba Dyak\GE22407 Drosophila ananassae Dana\GF24140 Drosophila pseudoobscura pseudoobscura Dpse\GA18860 Drosophila persimilis Dper\GL11911 Drosophila willistoni Dwil\GK17227 Drosophila virilis Dvir\GJ11840 Drosophila mojavensis Dmoj\GI13476 Drosophila grimshawi Dgri\GH16753
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	42523 y1 v1; P{TRiP.HMJ02089}attP40 41903 y1 sc* v1; P{TRiP.GL01337}attP2 41665 y1 sc* v1; P{TRiP.HMS02198}attP2 35056 y1 w*; Mi{MIC}trblMI01025/TM3, Sb1 Ser1
Harvard	- P{XP}d03175 - P{XP}trbld03251 - P{XP}trbld07751
Kyoto	113328 w*; P{GawB}trblNP3530 125051 w1118; P{RS5}trbl5-HA-1074
VDRC	v106774 P{KK108667}VIE-260B v22113 w1118; P{GD11640}v22113 v22114 w1118; P{GD11640}v22114
	More stocks available...
Genomic Clones ( 1 )
	BACR02B14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 183 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH69304
Other clones	DPiM_RH69304
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT16138 AT16267 AT23979 GH18593 GH18646 GH24136 HL05961 LP01915 LP22996 RE02504 RE02516 RE16969 RE46779 RE51057 RE65225 RH05553 RH06973 RH10675 RH19696 RH19714 RH29976 RH63314 RH68463 RH68814 SD01879 SD04278 SD15922
Other clones	69345 101997 167093 177243 177401 183392 281835 CK01.11A.F11 CK01.65A.G4 EC051018 EC052003 EC053046 EC055444 EC055447 EK053058 EK242632 EK272137 EK282226 EK288221 EK304107 EK307433 RH06707 RP003018066 RP003025515 RP003026152 RP003030841 RP003032659 RP003035189 RP003035284 RP003041929 RP003046895 RP003049039 RP003056323 RP003059343 RP003075736 RP003080349 RP003085612 RP003085726 RP003091816 RP003093417 RP003094247 RP003096145 RP003100287 RP003105999 RP003111197 RP003112555 RP003114813 RP003124948 RP003141305 RP003141670 RP003155215 RP003158810 RP003160420 RP003160808 RP003163986 RP003165054 RP003169194 RP003172603 RP003172845 RP003180610 RP003186005 RP003188112 RP003203063 RP003204912 RP003210640 RP003213010 RP003218715 RP003220624 RP003228137 RP003233851 RP003236102 RP003239272 RP003242080 RP003250443 RP003256103 RP003256742 RP003266082 RP003266515 RP003276971 RP003277785 RP003282856 RP003284218 RP003293078 RP003294016 RP003301291 RP003305345 RP003308532 RP003311891 RP003315648 RP003320438 RP003323425 RP003325642 RP003327472 RP003329998 RP003331123 RP003334605 RP003335839 RP003336070 RP003337567 RP003343295 RP003357928 RP003369421 RP003374637 RP003381207 RP003401085 RP003422363 RP003422565 RP003436908 RP003440513 RP003440733 RP003454723 RP003462014 RP003464360 RP003470299 RP003476778 RP003502006 RP003508172 RP003510691 RP003511128 RP003512466 RP003513731 RP003513856 RP003515066 RP003516225 RP003517959 RP003518134 RP003523741 RP003524942 RP003527677 RP003529095 RP003531697 RP003545192 RP003545198 RP003553970 RP003563262 RP003566227 RP003577618 RP003581705 RP003592777 RP003592955 RP003611339 RP003615864 RP003623583 RP003627471 RP003648092 RP003648225 RP003653107 RP003661358 RP003664226 RP003665201 RP003665895 RP003674133 RP003677058 RP003682090
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0028978 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43999
Antibody Information
	polyclonal (Masoner et al., 2013)
Other Information
Discoverer
Etymology
	The gene is named "tribbles" after the multiplying "tribbles" in "Star Trek". (Greaves, 2000) The gene tribbles is named after the fictional small round organisms that proliferate uncontrollably when they contact water. (Seher and Leptin, 2000)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: trbl CG5408 (Leptin, 2000.5.4, FlyBase, 1996-)
Additional comments	Source for merge of trbl CG5408 was sequence comparison (date:000516). (FlyBase, 1996-)
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells, a whole range of mitotic abnormalities and spindle abnormalities are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) This locus may be part of a mechanism that inhibits mitosis in the ventral furrow region as long as the morphogenetic movement takes place. (Grosshans and Wieschaus, 2000) In eggs that are deficient for this locus, the ventral cells divide even before the cells of the mitotic domain one, which are the first cells to go into the (first) zygotically controlled mitosis in wild-type eggs. (Grosshans and Wieschaus, 2000) trbl is required for the ventral inhibition of mitosis that acts during gastrulation to counteract stg specifically in the cells that invaginate to form the mesoderm. (Grosshans and Wieschaus, 2000) In embryos lacking trbl function, cells in the ventral domain are the first to enter mitosis, such that their pattern of stg RNA expression and the mitotic pattern match each other. (Grosshans and Wieschaus, 2000) trbl specifically regulates stg protein turnover. (Mata et al., 2000) Mutations in trbl cause premature division in the embryonic mesoderm anlage and gastrulation defects. (Mata et al., 2000) trbl is involved in directing the timing of mitosis in the mesoderm. (Seher and Leptin, 2000) In trbl mutant embryos the mesoderm becomes the first domain to divide, now following stg instructions just like every other domain does. The consequence of this premature division is a strong gastrulation phenotype. The trbl phenotype is caused solely by untimely mitosis. (Seher and Leptin, 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079933 RefSeq (Proteins) • NP_524672 DDBJ / EMBL / GenBank • AAF26374 AAF51590 AAO45190 AF204688 AI387940 AQ025182 AQ025311 BH614863 BI627841 BT004834 CZ473233 CZ473259 CZ475911 CZ476668 Entrez Gene - A searchable database of RefSeq genes. • 43999 UniProtKB/TrEMBL • Q9V3Z1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Pseudokinase tribbles family/serine-threonine-protein kinase 40 (IPR024104) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH69304
Linkouts
	BioGRID - A database of protein and genetic interactions • 68764 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028978 DroID - A comprehensive database of gene and protein interactions. • FBgn0028978 DRSC - Results from RNAi screens. • FBgn0028978 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028978 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5408-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0028978 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43999 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/tribbls1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43999 modMine - Data generated by the modENCODE project. • FBgn0028978 REDfly - A database of transcriptional regulatory elements. • FBgn0028978 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9V3Z1
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG5408 (Norga et al., 2003, Brody et al., 2002, Morrison et al., 2000, Leptin, 2000.5.4, Quinones-Coello, 2007, Keleman et al., 2009.8.5, Lowery et al., 2010, Ni et al., 2010.12.1) trb (Insco et al., 2009, Insco et al., 2009) trbl (LaFerriere et al., 2008, Mukherjee et al., 2006, Sandmann et al., 2007, Buszczak et al., 2007, Quinones-Coello, 2007, Seher et al., 2007, Axelsson et al., 2011, Choksi et al., 2006, Gawlinski et al., 2007, Rollmann et al., 2006, Liebl and Featherstone, 2005, Burgio et al., 2008, Lowery et al., 2010) unnamed (Grosshans and Wieschaus, 2000)
Name Synonym	tribbles (LaFerriere et al., 2008, Seher and Leptin, 2000, Seher, 1999.11.15, Leptin, 2005, Gregory et al., 2007, Seher et al., 2007, Becam et al., 2011, Morse et al., 2009, Gawlinski et al., 2007, Rollmann et al., 2006, Insco et al., 2009)
Secondary FlyBase IDs
	FBgn0036983
References ( 88 )
Generate a list of	All references relating to this gene ( 88 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 9 ) Review  ( 13 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 7 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Masoner et al., 2013, Dev. Biol. 375(1): 33--44 The kinase domain of Drosophila Tribbles is required for turnover of fly C/EBP during cellmigration. [FBrf0220729] Fisher et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(52): 21330--21335 DNA regions bound at low occupancy by transcription factors do not drive patterned reporter gene expression in Drosophila. [FBrf0220344] Axelsson et al., 2011, BMC Bioinformatics 12: 342 Extracting quantitative genetic interaction phenotypes from matrix combinatorial RNAi. [FBrf0215222] Becam et al., 2011, Development 138(17): 3781--3789 Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. [FBrf0214603] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...