• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\hbs

General Information
Symbol	Dmel\hbs	Species	D. melanogaster
Name	hibris	Annotation symbol	CG7449
Feature type	protein_coding_gene	FlyBase ID	FBgn0029082
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	icon
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	51D8-51D8	Sequence location	2R:10,898,901..10,928,883 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene hibris is referred to in FlyBase by the symbol Dmel\hbs (CG7449, FBgn0029082). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: Notch receptor processing; positive regulation of endopeptidase activity; homophilic cell adhesion; compound eye photoreceptor cell differentiation; regulation of striated muscle tissue development; positive regulation of Notch signaling pathway; Malpighian tubule stellate cell differentiation; establishment of ommatidial planar polarity; compound eye morphogenesis. 25 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; external compound sense organ; organ system; peripheral nervous system; adult segment; non-connected developing system; adult; extended germ band embryo; thoracic segment; histaminergic neuron. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: CD80-like, immunoglobulin C2-set; Fibronectin, type III; Immunoglobulin I-set; Immunoglobulin subtype; Immunoglobulin subtype 2; Immunoglobulin-like domain; Immunoglobulin-like fold; Immunoglobulin/major histocompatibility complex, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1637539_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of hbs. Gene sequence location is 2R:10898901..10928883.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Alleles hbs[DeltaICD.Scer\UAS.T:Ivir\HA1] hbs[Scer\UAS.cSa.T:Ivir\HA1] Transgenic Constructs P{UAS-hbs::sns.HESTC.HA} P{UAS-hbs::sns.SETHC.HA} P{UAS-hbs.DeltaICD.HA} P{UAS-hbs::sns.SEHTC.HA} P{UAS-hbs.HA.S} Controlled Vocabulary Terms protein binding heterophilic cell-cell adhesion
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 51D8-51D8   Limits computationally determined from genome sequence between P{lacW}Rpn6k00103 and P{PZ}chn02064
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51E1-51E2     (Artero, 1999.12.1)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\hbs for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) hbs-RA FBtr0087413  NM_079963   4701   1228 hbs-RB FBtr0087414  NM_176175   4722   1235
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein hbs-PA   FBpp0086544   133.6   1228   7.00     NP_524702   AAF58172 hbs-PB   FBpp0086545   134.4   1235   7.00     NP_788355   AAO41390
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin/major histocompatibility complex, conserved site (IPR003006) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Fibronectin, type III (IPR003961) Immunoglobulin-like domain (IPR007110) CD80-like, immunoglobulin C2-set (IPR013162) Immunoglobulin-like fold (IPR013783) Immunoglobulin I-set (IPR013098)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698209   AE013599 AAF58172   AE013599 AAO41390   AF210316 AAF19446   AI516976   AI533599   AI533610   AI542377   AI542378   AY121698 AAM52025   BI371939   BI577159   BI638603   BT083423   BT083424   CO153583   CO153587   CO154719   CO180997   CO185385   CO263568   CO271100   CO276122   CO276763   CO278242   CO278634   CO281433   CO287862   CO291292   CO295533   CO299475   CO306158   CO311708   CO315467   CO316697   CO328651   CO333095   CO338252   CO338595   CO341146   CO342686   CO342933   CO345052   EC066549   EC067908   EC071657   EC196596   EC197456   EC199717   EC209563   EC213650   EC221488   EC231911   EC242431   EC259481   EC260543   EV585895   GH944940   GH946970   GH947817   GH948833   GH948853   GH951504   GH951899   GH953762   GH954750   GH955123   GH958698   GH961604   GH962704   GH962901   GH962958   GH964123   GH964548   GH966155   GH966839   GH968267   GH969147   GH969238   GH970093   GH973969   GH977809   GH980699   GH982240
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MRA3 A1Z9X4 Q9V787
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15   embryonic Malpighian tubule | restricted   (Denholm et al., 2003)
Additional Descriptive Data
	Expression of the hbs protein was detected in stage 15 embryonic Malpighian tubule stellate cells. (Denholm et al., 2003)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
apical plasma membrane inferred from direct assay (Singh and Mlodzik, 2012) colocalizes_with endosome inferred from direct assay (Singh and Mlodzik, 2012) colocalizes_with gamma-secretase complex inferred from physical interaction with Psn (Singh and Mlodzik, 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    144.8 Larval Midgut    1.6 Larval Hindgut    7.3 Larval Malpighian Tubules    3.1 Larval Fat Body    3.6 Larval Salivary Gland    5.5 Larval Trachea    49.675 Larval Carcass    20.925 Adult Head    28.4 Adult Eye    28.275 Adult Brain    124.8 Adult Thoracic-Abdominal Ganglion    98.2 Adult Crop    2.9 Adult Midgut    1.1 Adult Hindgut    4.3 Adult Malpighian Tubules    4 Adult Fat Body    10.3 Adult Salivary Gland    13.2 Adult Heart    1.025 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    13.5 Adult Ovary    0.5 Adult Testis    1.6 Adult Male Accessory Gland    5.9 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    28.4   NA  Eye    28.275   NA  Brain    124.8   144.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    98.2   NA  Crop    2.9   1.6  Midgut    1.1   7.3  Hindgut    4.3   3.1  Malpighian Tubules    4   3.6  Fat Body    no informative data   5.5  Salivary Gland    no informative data   NA  Heart    1.025   49.675  Trachea    NA   NA  VirginFemale Spermatheca    5.7   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    0.5   NA  Testis    1.6   NA  Male Accessory Gland    5.9   20.925  Carcass    3.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    17 central nervous system, pupae P8    25 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    4 head, mated 4-day male    3 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    11 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr  (32) embryo 08-10hr  (35) embryo 10-12hr  (30) embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr  (29) pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr  32 embryo 08-10hr  (35) embryo 10-12hr  30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr  29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    4 embryo 04-06hr    15 embryo 06-08hr    32 embryo 08-10hr    35 embryo 10-12hr    30 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    22 embryo 18-20hr    18 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    9 white prepupae 12hr    16 white prepupae 24hr    29 pupae 2d postWPP    24 pupae 3d postWPP    11 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029082 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7449-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 hbsGD2566 viable hbsc06523 viable, with Scer\GAL4pnr-MD237 hbsGD70 hbsGD14406 Sterility Allele fertile hbsc06523 Other Phenotypes Allele cell adhesion defective | somatic clone, with Scer\GAL4GMR.PF hbsScer\UAS.cDa planar polarity defective, with Scer\GAL4hs.2sev hbsScer\UAS.cDa hbsVDRC.cUa planar polarity defective (with Df(2R)δ12) hbs1 hbs66 hbsEP planar polarity defective (with hbs459), with Scer\GAL4hs.2sev hbsVDRC.cUa planar polarity defective (with hbsVDRC.cUa), with Scer\GAL4hs.2sev hbs459 planar polarity defective | recessive hbs1 hbs66 hbsEP visible, with Scer\GAL4bi-md653 hbsScer\UAS.cDa visible, with Scer\GAL4da.G32 hbsScer\UAS.cDa visible, with Scer\GAL4en-e16E hbsScer\UAS.cDa hbsVDRC.cUa visible, with Scer\GAL4GMR.PF hbsScer\UAS.cDa visible, with Scer\GAL4pnr-MD237 hbsScer\UAS.cDa hbsVDRC.cUa visible, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa visible (with Df(2R)ED2423) hbs459 visible (with hbs1) hbs459 hbs2593 visible (with hbs66) hbs459 hbs2593 visible (with hbs459) hbs1 hbs66 visible (with hbs2593) hbs1 hbs66 visible | recessive hbs1 hbs66 Phenotype manifest in Allele adult Malpighian tubule Type II cell hbs361 cell-cell junction | somatic clone, with Scer\GAL4Act5C.PI hbsScer\UAS.cDa cone cell, with Scer\GAL4GMR.PF hbsdsRNA.Scer\UAS.cBa cone cell | adult stage hbs66 hbs1 hbsEP cone cell | adult stage (with Df(2R)δ12) hbs66 hbs1 hbsEP cone cell | adult stage | somatic clone hbsEP cone cell | pharate adult stage hbs66 hbs1 hbsEP cone cell | pharate adult stage (with Df(2R)δ12) hbs66 hbs1 hbsEP cone cell | pharate adult stage | somatic clone hbsEP embryonic myoblast (with Df(2R)X28) hbs2593 hbs459 embryonic myoblast (with hbs459) hbs2593 embryonic myoblast (with hbs2593) hbs459 eye hbs66 hbs1 eye, with Scer\GAL4da.G32 hbsScer\UAS.cDa eye, with Scer\GAL4GMR.PF hbsScer\UAS.cDa hbsdsRNA.Scer\UAS.cBa eye, with Scer\GAL4hs.2sev hbsScer\UAS.cDa hbsVDRC.cUa eye, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa eye (with hbs1) hbs2593 hbs459 eye (with hbs66) hbs2593 hbs459 eye (with hbs459) hbs66 hbs1 eye (with hbs459), with Scer\GAL4hs.2sev hbsVDRC.cUa eye (with hbs2593) hbs66 hbs1 eye (with hbsVDRC.cUa), with Scer\GAL4hs.2sev hbs459 eye | somatic clone, with Scer\GAL4GMR.PF hbsScer\UAS.cDa eye photoreceptor cell, with Scer\GAL4hs.2sev hbsScer\UAS.cDa interommatidial bristle, with Scer\GAL4da.G32 hbsScer\UAS.cDa interommatidial bristle, with Scer\GAL4GMR.PF hbsScer\UAS.cDa hbsdsRNA.Scer\UAS.cBa interommatidial bristle, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa interommatidial bristle | pupal stage P6, with Scer\GAL4GMR.PF hbsdsRNA.Scer\UAS.cBa interommatidial bristle | supernumerary hbsEP inter-ommatidial cell, with Scer\GAL4GMR.PF hbsdsRNA.Scer\UAS.cBa inter-ommatidial cell | somatic clone, with Scer\GAL4GMR.PF hbsScer\UAS.cDa inter-ommatidial precursor cell, with Scer\GAL4GMR.PF hbsdsRNA.Scer\UAS.cBa inter-ommatidial precursor cell | P-stage | somatic clone, with Scer\GAL4Act5C.PI hbsScer\UAS.cDa inter-ommatidial precursor cell | pupal stage P6, with Scer\GAL4GMR.PF hbsdsRNA.Scer\UAS.cBa inter-ommatidial precursor cell | somatic clone, with Scer\GAL4GMR.PF hbsScer\UAS.cDa microchaeta & adult thorax hbsEP microchaeta & scutum, with Scer\GAL4da.G32 hbsScer\UAS.cDa microchaeta & scutum, with Scer\GAL4pnr-MD237 hbsScer\UAS.cDa microchaeta & scutum, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa midgut chamber 1 (with Df(2R)X28) hbs2593 hbs459 midgut chamber 1 (with hbs459) hbs2593 midgut chamber 1 (with hbs2593) hbs459 myoblast, with Scer\GAL4da.G32 hbsScer\UAS.cDa myoblast, with Scer\GAL4en-e16E hbsScer\UAS.cDa myoblast, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB hbsΔECD.Scer\UAS hbsScer\UAS.cAa myoblast, with Scer\GAL4pnr-MD237 hbsScer\UAS.cDa myoblast, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa myoblast, with Scer\GAL4twi.PG hbsScer\UAS.cDa notal microchaeta, with Scer\GAL4pnr-MD237 hbsVDRC.cUa notal microchaeta (with Df(2R)ED2423) hbs459 ommatidium, with Scer\GAL4da.G32 hbsScer\UAS.cDa ommatidium, with Scer\GAL4GMR.PF hbsScer\UAS.cDa hbsdsRNA.Scer\UAS.cBa ommatidium, with Scer\GAL4hs.2sev hbsScer\UAS.cDa hbsVDRC.cUa ommatidium, with Scer\GAL4hs.2sev, hbs459 hbsVDRC.cUa ommatidium, with Scer\GAL4hs.2sev, hbsVDRC.cUa hbs459 ommatidium, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa ommatidium | somatic clone, with Scer\GAL4GMR.PF hbsScer\UAS.cDa photoreceptor cell R3, with Scer\GAL4hs.2sev hbsVDRC.cUa photoreceptor cell R3, with Scer\GAL4hs.2sev, hbs459 hbsVDRC.cUa photoreceptor cell R3, with Scer\GAL4hs.2sev, hbsVDRC.cUa hbs459 photoreceptor cell R7, with Scer\GAL4hs.2sev hbsVDRC.cUa photoreceptor cell R7, with Scer\GAL4hs.2sev, hbs459 hbsVDRC.cUa photoreceptor cell R7, with Scer\GAL4hs.2sev, hbsVDRC.cUa hbs459 presumptive embryonic/larval muscle system, with Scer\GAL4da.G32 hbsScer\UAS.cDa presumptive embryonic/larval muscle system, with Scer\GAL4en-e16E hbsScer\UAS.cDa presumptive embryonic/larval muscle system, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB hbsΔECD.Scer\UAS hbsScer\UAS.cAa presumptive embryonic/larval muscle system, with Scer\GAL4sca-537.4 hbsScer\UAS.cDa presumptive embryonic/larval muscle system (with Df(2R)X28) hbs2593 hbs459 presumptive embryonic/larval muscle system (with hbs459) hbs2593 presumptive embryonic/larval muscle system (with hbs2593) hbs459 presumptive embryonic/larval muscle system | dorsal, with Scer\GAL4pnr-MD237 hbsScer\UAS.cDa primary pigment cell | P-stage | somatic clone, with Scer\GAL4Act5C.PI hbsScer\UAS.cDa septate junction | somatic clone, with Scer\GAL4Act5C.PI hbsScer\UAS.cDa somatic mesoderm, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB hbsΔECD.Scer\UAS hbsScer\UAS.cAa visceral mesoderm, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB hbsΔECD.Scer\UAS hbsΔICD.Scer\UAS hbsScer\UAS.cAa visceral muscle primordium (with Df(2R)X28) hbs2593 hbs459 visceral muscle primordium (with hbs459) hbs2593 visceral muscle primordium (with hbs2593) hbs459 wing hair, with Scer\GAL4neur-GAL4-A101 hbsKK106248 wing margin, with Scer\GAL4en-e16E hbsScer\UAS.cDa hbsVDRC.cUa wing margin, with Scer\GAL4neur-GAL4-A101 hbsKK106248 wing margin | distal, with Scer\GAL4bi-md653 hbsScer\UAS.cDa wing margin | distal, with Scer\GAL4da.G32 hbsScer\UAS.cDa
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of hbs Class Mutagen Stocks Known lesion hbs1 X ray 1 Yes hbs66 hypomorphic allele - genetic evidence X ray 1 Yes hbsc01977 1 -- hbsc06523 piggyBac transposase 1 -- hbsEP 1 Yes hbsMB10413 1 -- hbsMI02141 1 -- hbsMI02315 1 -- hbsMI04194 1 -- hbs2593 ethyl methanesulfonate 0 Yes hbs361 loss of function allele ethyl methanesulfonate 0 -- hbs459 loss of function allele ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of hbs Class Mutagen Stocks Known lesion hbsGD2566 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes hbsGD14406 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hbsGD70 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hbsKK106248 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hbsScer\UAS.cDa in vitro construct - regulatory fusion 1 Yes hbsScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes hbsdsRNA.Scer\UAS.cBa in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes hbsdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes hbsMtnA.PD in vitro construct - regulatory fusion 0 Yes hbsScer\UAS.cAa in vitro construct - regulatory fusion 0 Yes hbsScer\UAS.cSa.T:Ivir\HA1 0 Yes hbsVDRC.cUa in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes hbsΔECD.Scer\UAS in vitro construct - deletion 0 Yes hbsΔICD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - coding region fusion 0 Yes hbsΔICD.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)EP2590-Δ1 (Dworak et al., 2001) Df(2R)EP2590-Δ14 (Dworak et al., 2001, Denholm et al., 2003) Df(2R)δ12 (Grillo-Hill and Wolff, 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD70} NA P{GD2566} NA P{GD14406} NA P{KK106248} NA P{UAS-hbs.A} NA P{UAS-hbs.D} NA P{UAS-hbs.HA.S} NA P{UAS-hbs.HA} NA P{UAS-hbs.IR} NA P{UAS-hbs.ΔECD} NA P{UAS-hbs.ΔICD.HA} NA P{UAS-hbs.ΔICD} NA reporter construct P{hbs-lacZ.4} No characterization construct P{UAS-hbs::sns.HESTC.HA} NA P{UAS-hbs::sns.SEHTC.HA} NA P{UAS-hbs::sns.SETHC.HA} NA P{VDRC.hbs.U} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}hbsMI02141 NA Mi{MIC}hbsMI02315 NA Mi{MIC}hbsMI04194 NA P{hbs-lacZ.4}36.1 NA PBac{PB}hbsc01977 NA PBac{PB}hbsc06523 NA insertion of mobile activating element P{Mae-GMR.EP}hbsEP NA insertion of enhancer trap binary system Mi{ET1}hbsMB10413 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with sns (Shelton et al., 2009)
Biological Process
CV term Evidence References
compound eye morphogenesis inferred from mutant phenotype (Bao et al., 2010) compound eye photoreceptor cell differentiation inferred from mutant phenotype (Singh and Mlodzik, 2012) establishment of ommatidial planar polarity inferred from mutant phenotype (Singh and Mlodzik, 2012) heterophilic cell-cell adhesion inferred from direct assay (Shelton et al., 2009) homophilic cell adhesion inferred from genetic interaction with rst (Zhuang et al., 2009) Malpighian tubule stellate cell differentiation inferred from expression pattern (Liu et al., 1999) inferred from mutant phenotype (Liu et al., 1999) myoblast fusion inferred from genetic interaction with sns (Shelton et al., 2009) Notch receptor processing inferred from mutant phenotype (Singh and Mlodzik, 2012) positive regulation of endopeptidase activity inferred from mutant phenotype (Singh and Mlodzik, 2012) positive regulation of Notch signaling pathway inferred from mutant phenotype (Singh and Mlodzik, 2012) regulation of striated muscle tissue development inferred from genetic interaction with sns AND inferred from mutant phenotype (Artero et al., 2001) inferred from mutant phenotype (Dworak et al., 2001)
Cellular Component
CV term Evidence References
apical plasma membrane inferred from direct assay (Singh and Mlodzik, 2012) colocalizes_with endosome inferred from direct assay (Singh and Mlodzik, 2012) colocalizes_with gamma-secretase complex inferred from physical interaction with Psn (Singh and Mlodzik, 2012)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
myoblast fusion traceable author statement (Dworak and Sink, 2002)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity with NCBI_gi:4758822 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
hbs - CG15098 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) hbs - Hsc70-3 experimental knowledge based (Guruharsha et al., 2011) hbs - RpS24 experimental knowledge based (Guruharsha et al., 2011) hbs - Syb anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions hbs allele Gene References hbs459 sns (Artero et al., 2001) hbs2593 sns (Artero et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68850 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029082 DroID - A comprehensive database of gene and protein interactions. • FBgn0029082 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44129
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6X0NBN Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG63TXQB Arthropod inclusive ortholog search EOG6TB2S4 Metazoa inclusive ortholog search EOG69PPRN
Orthologs in Drosophila Species (EOG6X0NBN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\hbs     Drosophila simulans   Dsim\GD11096 Y   Drosophila sechellia   Dsec\GM21588 Y   Drosophila erecta   Dere\GG20496 Y   Drosophila yakuba   Dyak\GE13629 Y   Drosophila ananassae   Dana\GF12310 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20361 Y   Drosophila persimilis   Dper\GL17448 Y   Drosophila willistoni   Dwil\GK19700 Y   Drosophila virilis   Dvir\GJ21730 Y   Drosophila mojavensis   Dmoj\GI18711 Y   Drosophila grimshawi   Dgri\GH21109 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG63TXQB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB47244 Y Apis mellifera Western honey bee  Amel\GB47243 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014561 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12451 Y Atta cephalotes Leafcutter ant  Acep\ACEP14427 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO13639 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18337 Y Linepithema humile Argentine ant  Lhum\LH20072 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB12441 Y Solenopsis invicta Red fire ant  Sinv\SINV21545 Y Solenopsis invicta Red fire ant  Sinv\SINV21514 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI007875 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000162 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000164 Y Pediculus humanus Human body louse  Phum\PHUM068980 Y Pediculus humanus Human body louse  Phum\PHUM623080 Y Tribolium castaneum Red flour beetle  Tcas\TC003361 Y
Orthologs in non-Insect Arthropods (EOG6TB2S4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_305049 Y Ixodes scapularis Deer tick  Isca\ISCW022951 Y Ixodes scapularis Deer tick  Isca\ISCW014027 Y
Orthologs in non-Arthropod Metazoa (EOG69PPRN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\syg-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009318gn Y Danio rerio Zebrafish  Drer\nphs1l Y Xenopus tropicalis Western clawed frog  Xtro\nphs1 Y Mus musculus House mouse  Mmus\Nphs1 Y Rattus norvegicus Norway rat  Rnor\Nphs1 Y Homo sapiens Human  Hsap\NPHS1 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\NPHS1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11096 Drosophila sechellia Dsec\GM21588 Drosophila erecta Dere\GG20496 Drosophila yakuba Dyak\GE13629 Drosophila ananassae Dana\GF12310 Drosophila pseudoobscura pseudoobscura Dpse\GA20361 Drosophila persimilis Dper\GL17448 Drosophila willistoni Dwil\GK19700 Drosophila virilis Dvir\GJ21730 Drosophila mojavensis Dmoj\GI18711 Drosophila grimshawi Dgri\GH21109
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	41797 w*; P{UAS-hbs.D}7.1 17767 w1118; PBac{PB}hbsc06523 29893 w1118; Mi{ET1}hbsMB10413 27618 w1118; P{neoFRT}42D hbs66/CyO 41796 w*; P{UAS-hbs.HA}14.1/TM6B, Tb1 35969 y1 w*; Mi{MIC}hbsMI02141
Harvard	- PBac{PB}hbsc01977
VDRC	v105913 P{KK106248}VIE-260B v40896 w1118 P{GD2566}v40896 v40898 w1118; P{GD2566}v40898 v9471 w1118; P{GD70}v9471/CyO v27065 w1118; P{GD14406}v27065/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR01L16 BACR30C07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 80 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE70806
Other clones	DPiM_RE70806 RT02618 RT02621
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH27590 HL03950 RE58973 SD05488 SD05505 SD08671 SD08672 SD20937
Other clones	517 15256 17298 47451 102563 162215 214439.86 214473.77 281014 CK01.100B.D12 CK01.133B.E8 CK01.141D.G1 Dmel_dig_012975_0584_2648 EC13319 EK008922 EK067002 EK077346 EK088936 EK101952 EK119732 EK122014 EK129422 EK130640 EK157147 EK175525 EK210625 EK225949 EK250406 EK261553 EK265654 EK291057 EK305655 EN02401 EN02405 EN04650 EN16322 EN17143 EP11939 EP15334 EP15755 EP21212 RP004026260 RP004103374 RP004108423 RP004125239 RP004151988 RP004157288 RP004193367 RP004193687 RP004204717 RP004213894 RP004223280 RP004234281 RP004238769 RP004278636 RP004372824 RP004432370 RP004444115 RP004445640 RP004457389 RP004474914 RP004482712 RP004502566 RP004507996 RP004519411 RP004519898 RP004522423 RP004534066
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0029082 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44129
Antibody Information
	polyclonal (Bao et al., 2010)
Other Information
Discoverer
Etymology
	The free myoblasts of the hbs mutant phenotype represent rebelliousness, insolence, and transgression of rules (in this case in the mesodermal "society"), the three qualities that the minor Greek Goddess "hibris" represents. (Artero and Baylies, 2001.5.25)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: hbs CG7449 (Artero, 2000.3.29) Source for merge of: hbs icon (Grillo-Hill, 2005.3.28)
Additional comments
Other Comments
	ChEST reveals this is a target of Mef2. (Junion et al., 2005) Mutants exhibit a phenotype in which the A/P cone cell contact is improperly maintained through the end of pattern formation. (Grillo-Hill and Wolff, 2003) Embryos mutant for hbs or overexpressing hbs show a partial myoblast fusion block. (Artero and Baylies, 2001.5.25) hbs appears to function as a dose dependant regulator of myoblast fusion. (Artero et al., 2001) hbs appears to be a component of the molecular machinery that facilitates both myoblast and myotube guidance. hbs may also be involved in cell-cell contact events in the eye, bristle sensory oprgan and wing development. (Dworak et al., 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079963 NM_176175 RefSeq (Proteins) • NP_524702 NP_788355 DDBJ / EMBL / GenBank • AAF19446 AAF58172 AAF58172.3 AAM52025 AAO41390 AAO41390.1 AF210316 AY121698 BI577159 Entrez Gene - A searchable database of RefSeq genes. • 44129 UniProtKB/TrEMBL • A1Z9X4 Q8MRA3 Q9V787
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin/major histocompatibility complex, conserved site (IPR003006) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Fibronectin, type III (IPR003961) Immunoglobulin-like domain (IPR007110) CD80-like, immunoglobulin C2-set (IPR013162) Immunoglobulin-like fold (IPR013783) Immunoglobulin I-set (IPR013098)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68850 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029082 DroID - A comprehensive database of gene and protein interactions. • FBgn0029082 DRSC - Results from RNAi screens. • FBgn0029082 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029082 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7449-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0029082 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44129 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/hibris1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44129 modMine - Data generated by the modENCODE project. • FBgn0029082
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG7449 (Deloger et al., 2009, Krejcí et al., 2009, Keleman et al., 2009.8.5) CT22841 (Hynes and Zhao, 2000) Hbs (Artero et al., 2001, Linneweber and Fischbach, 2007, Chen et al., 2007, Deloger et al., 2009, Höhne et al., 2011, Helmstadter et al., 2012, Johnson et al., 2008, Bao et al., 2010) hbs (Menon et al., 2005, Durupt et al., 2007, Takemura and Adachi-Yamada, 2011, Southall and Brand, 2009, Sugie et al., 2010, Susic-Jung et al., 2012, Johnson et al., 2011, Johnson et al., 2008, Weavers et al., 2009, Krejcí et al., 2009, Shelton et al., 2009, Zhuang et al., 2009, Grillo-Hill and Wolff, 2009, Bao and Cagan, 2005, Wheeler et al., 2009, Bao et al., 2010, Boy et al., 2010, Artero et al., 2006, Weber, 2012.9.7, Singh and Mlodzik, 2012) icon (Grillo-Hill and Wolff, 2003, Grillo-Hill and Wolff, 2009)
Name Synonym	hibris (Grillo-Hill, 2005.3.28, Artero, 2000.3.29, Artero, 1999.12.1, Durupt et al., 2007, Weavers et al., 2009, Krejcí et al., 2009, Grillo-Hill and Wolff, 2009, Bao and Cagan, 2006, Bao and Cagan, 2005, Boy et al., 2010, Singh and Mlodzik, 2012, Manning et al., 2012) Hibris (Tepass and Harris, 2007, Rochlin et al., 2007, LeBrasseur, 2005, Chen et al., 2007, Johnson et al., 2011, Johnson et al., 2008, Bao et al., 2010) inappropriate contact (Grillo-Hill and Wolff, 2003) inappropriate contacts (Grillo-Hill and Wolff, 2005, Grillo-Hill and Wolff, 2009)
Secondary FlyBase IDs
	FBgn0033991 FBgn0064179
References ( 88 )
Generate a list of	All references relating to this gene ( 88 )
List References by type	Research paper  ( 37 ) Supplementary material  ( 6 ) Review  ( 8 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 16 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Note  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 11 )
	Housden et al., 2013, PLoS Genet. 9(1): e1003162 Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. [FBrf0220474] Kaipa et al., 2013, J. Cell Sci. 126(1): 360--372 Dock mediates Scar- and WASp-dependent actin polymerization through interaction with cell adhesion molecules in founder cells and fusion-competent myoblasts. [FBrf0221091] Gildor et al., 2012, Development 139(21): 4040--4050 Bidirectional Notch activation represses fusion competence in swarming adult Drosophila myoblasts. [FBrf0219658] Helmstadter et al., 2012, PLoS ONE 7(7): e40300 Functional Study of Mammalian Neph Proteins in Drosophila melanogaster. [FBrf0218912] Manning et al., 2012, Cell Rep. 2(4): 1002--1013 A Resource for Manipulating Gene Expression and Analyzing cis-Regulatory Modules in the Drosophila CNS. [FBrf0219785] Singh and Mlodzik, 2012, Dev. Cell 23(1): 82--96 Hibris, a Drosophila Nephrin Homolog, Is Required for Presenilin-Mediated Notch and APP-like Cleavages. [FBrf0218978] Susic-Jung et al., 2012, Dev. Biol. 370(1): 86--97 Multinucleated smooth muscles and mononucleated as well as multinucleated striated muscles develop during establishment of the male reproductive organs of Drosophila melanogaster. [FBrf0219326] Höhne et al., 2011, Mol. Cell. Biol. 31(16): 3241--3251 The BAR Domain Protein PICK1 Regulates Cell Recognition and Morphogenesis by Interacting with Neph Proteins. [FBrf0214537] Johnson et al., 2011, Mol. Biol. Cell 22(23): 4513--4526 Role for a Cindr-Arf6 axis in patterning emerging epithelia. [FBrf0216708] Muñoz-Soriano et al., 2011, Fly 5(2): 88--101 Mtl interacts with members of Egfr signaling and cell adhesion genes in the Drosophila eye. [FBrf0213803] Takemura and Adachi-Yamada, 2011, Dev. Biol. 357(2): 336--346 Cell death and selective adhesion reorganize the dorsoventral boundary for zigzag patterning of Drosophila wing margin hairs. [FBrf0214791] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...