Gene Dmel\Uba2

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General Information

Symbol

Dmel\Uba2

Species

D. melanogaster

Name

Smt3 activating enzyme 2

Annotation symbol

CG7528

Feature type

protein_coding_gene

FlyBase ID

FBgn0029113

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

SAE2, sip2, DmUba2, DmSae2

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

66B7-66B7

Sequence location

3L:8,118,427..8,120,918 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Smt3 activating enzyme 2 is referred to in FlyBase by the symbol Dmel\Uba2 (CG7528, FBgn0029113). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: humoral immune response; protein sumoylation; positive regulation of NF-kappaB transcription factor activity. 15 alleles are reported. The phenotypes of these alleles are annotated with: eye disc; eye; optic lobe; adult head; embryonic/larval fat body; imaginal disc; ommatidium; embryonic/larval hemocoel; pigment cell. It has one annotated transcript and one annotated polypeptide. Protein features are: Molybdenum cofactor biosynthesis, MoeB; NAD(P)-binding domain; UBA/THIF-type NAD/FAD binding fold; Ubiquitin-activating enzyme; Ubiquitin-activating enzyme repeat; Ubiquitin-activating enzyme, E1, active site; Ubiquitin-like 1 activating enzyme, catalytic cysteine domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631381_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Uba2. Gene sequence location is 3L:8118427..8120918.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66B7-66B7

Limits computationally determined from genome sequence between P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118 and P{lacW}Nmt^j1C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65B5-65C3

(determined by in situ hybridisation)

(Long and Griffith, 2000)

66B6-66B11

(Shih, 1999.10.8)

66B5-66B11

Cytological location based on P1 blot screening and PCR checking.

(Shih, 1998.3.17)

66B6-66B10

Mapped using a high-density filter of P1 clones.

(Shih et al., 2002)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Uba2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Uba2-RA

FBtr0076734

NM_080017

2329

700

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Uba2-PA

FBpp0076457

77.6

700

4.63

NP_524756

AAF50484

Additional Polypeptide Data & Comments

Reported size (kDa)

700 (aa)

(Bhaskar et al., 2000)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-activating enzyme, E1, active site (IPR018074)

Ubiquitin-activating enzyme repeat (IPR000127)

UBA/THIF-type NAD/FAD binding fold (IPR000594)

Molybdenum cofactor biosynthesis, MoeB (IPR009036)

Ubiquitin-activating enzyme (IPR019572)

NAD(P)-binding domain (IPR016040)

Ubiquitin-like 1 activating enzyme, catalytic cysteine domain (IPR023280)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic cycle 10 -- embryonic cycle 14

organism

• nucleus

(Shih et al., 2002)

Comment:interphase

• cell cortex

(Shih et al., 2002)

Comment:mitosis

embryonic stage 6 -- 15

organism

• cytoplasm

(Shih et al., 2002)

Comment:mitosis

organism

• nucleus

(Shih et al., 2002)

Comment:interphase

adult stage

germarium

• nucleus

(Shih et al., 2002)

oocyte

• nucleus

(Shih et al., 2002)

Additional Descriptive Data

During embryonic cycles 10-14 protein was detected distributes around the cortex during mitotic divisions but concentrated in the nuclei during interphase. In later embryos postcellularization protein was detected in the cytoplasm of mitotic cells and primarily in the nuclei of interphase cells. No substantial colocalization with Septins was observed. During oogenesis Uba2 protein was detected in the nuclei of oocytes and of the follicle cells in the germarium.

(Shih et al., 2002)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Shih et al., 2002)

nucleus

inferred from direct assay

(Shih et al., 2002, Smith et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1308.55
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1308.55)
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		(572.2)
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		(1000.7)
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1308.55)
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1308.55
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1308.55
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1308.55)
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		(1000.7)
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1308.55
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1308.55
Larval Midgut		101.4
Larval Hindgut		388.1
Larval Malpighian Tubules		279.5
Larval Fat Body		257.3
Larval Salivary Gland		572.2
Larval Trachea		535.175
Larval Carcass		365.375
Adult Head		195.4
Adult Eye		257.25
Adult Brain		243.5
Adult Thoracic-Abdominal Ganglion		269.8
Adult Crop		307
Adult Midgut		136.7
Adult Hindgut		252.1
Adult Malpighian Tubules		208.7
Adult Fat Body		140.6
Adult Salivary Gland		133.4
Adult Heart		194.2
Adult VirginFemale Spermatheca		135.6
Adult InseminatedFemale Spermatheca		133
Adult Ovary		1000.7
Adult Testis		202.2
Adult Male Accessory Gland		216.4
Adult Carcass		154.4
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	195.4
NA	Eye	257.25
NA	Brain	243.5
1308.55	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	269.8
NA	Crop	307
101.4	Midgut	136.7
388.1	Hindgut	252.1
279.5	Malpighian Tubules	208.7
257.3	Fat Body	140.6
572.2	Salivary Gland	133.4
NA	Heart	194.2
535.175	Trachea	NA
NA	VirginFemale Spermatheca	135.6
NA	InseminatedFemale Spermatheca	133
NA	Ovary	1000.7
NA	Testis	202.2
NA	Male Accessory Gland	216.4
365.375	Carcass	154.4

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(65)
central nervous system, larvae L3		(80)
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		(63)
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		(91)
ovary, mated 4-day female		(90)
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(65)
central nervous system, larvae L3		(80)
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		(63)
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		(91)
ovary, mated 4-day female		(90)
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		65
central nervous system, larvae L3		80
central nervous system, pupae P8		19
head, virgin 1-day female		7
head, virgin 4-day female		8
head, virgin 20-day female		7
head, mated 1-day female		8
head, mated 4-day female		8
head, mated 20-day female		8
head, mated 1-day male		8
head, mated 4-day male		13
head, mated 20-day male		16
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		13
digestive system, 1-day adult		11
digestive system, 4-day adult		13
digestive system, 20-day adult		12
fat body, larvae L3 wandering		6
fat body, white prepupae		15
fat body, pupae P8		19
carcass, larvae L3 wandering		63
carcass, 1-day adult		7
carcass, 4-day adult		12
carcass, 20-day adult		10
ovary, virgin 4-day female		91
ovary, mated 4-day female		90
testis, mated 4-day male		22
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		135
embryo 02-04hr		166
embryo 04-06hr		120
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(135)
embryo 02-04hr		(166)
embryo 04-06hr		(120)
embryo 06-08hr		(104)
embryo 08-10hr		(102)
embryo 10-12hr		(71)
embryo 12-14hr		(54)
embryo 14-16hr		(47)
embryo 16-18hr		(37)
embryo 18-20hr		17
embryo 20-22hr		(47)
embryo 22-24hr		(43)
larva L1		24
larva L2		(31)
larva L3 12hr old		26
larva L3 puffstage 1-2		(34)
larva L3 puffstage 3-6		(47)
larva L3 puffstage 7-9		(60)
white prepupae new		(55)
white prepupae 12hr		(68)
white prepupae 24hr		(80)
pupae 2d postWPP		(32)
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		(47)
adult female 05day		(87)
adult female 30day		(86)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(135)
embryo 02-04hr		(166)
embryo 04-06hr		(120)
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		135
embryo 02-04hr		166
embryo 04-06hr		120
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		135
embryo 02-04hr		166
embryo 04-06hr		120
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(135)
embryo 02-04hr		(166)
embryo 04-06hr		(120)
embryo 06-08hr		(104)
embryo 08-10hr		(102)
embryo 10-12hr		(71)
embryo 12-14hr		(54)
embryo 14-16hr		(47)
embryo 16-18hr		(37)
embryo 18-20hr		17
embryo 20-22hr		(47)
embryo 22-24hr		(43)
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		(34)
larva L3 puffstage 3-6		(47)
larva L3 puffstage 7-9		(60)
white prepupae new		(55)
white prepupae 12hr		(68)
white prepupae 24hr		(80)
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		(47)
adult female 05day		(87)
adult female 30day		(86)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		135
embryo 02-04hr		(166)
embryo 04-06hr		120
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		135
embryo 02-04hr		166
embryo 04-06hr		120
embryo 06-08hr		104
embryo 08-10hr		102
embryo 10-12hr		71
embryo 12-14hr		54
embryo 14-16hr		47
embryo 16-18hr		37
embryo 18-20hr		17
embryo 20-22hr		47
embryo 22-24hr		43
larva L1		24
larva L2		31
larva L3 12hr old		26
larva L3 puffstage 1-2		34
larva L3 puffstage 3-6		47
larva L3 puffstage 7-9		60
white prepupae new		55
white prepupae 12hr		68
white prepupae 24hr		80
pupae 2d postWPP		32
pupae 3d postWPP		14
pupae 4d postWPP		13
adult male 01day		15
adult male 05day		18
adult male 30day		17
adult female 01day		47
adult female 05day		87
adult female 30day		86
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		142
Schneider line 2 Sg4		121
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		134
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		110
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		126
ovary OSC		94
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(142)
Schneider line 2 Sg4		(121)
embryonic 1182-4H		(89)
embryonic GM2		(50)
embryonic Kc167		(43)
embryonic S1		(83)
embryonic S3		(134)
leg disc CME L1		(105)
wing disc CME-W2		(75)
wing disc ML-DmD8		(84)
wing disc ML-DmD9		(101)
wing disc ML-DmD16-c3		(102)
wing disc ML-DmD21		(110)
wing disc ML-DmD32		(50)
haltere disc ML-DmD17-c3		(105)
eye-antennal disc ML-DmD11		(62)
antennal disc ML-DmD20-c5		(89)
mixed discs ML-DmD4-c1		(92)
CNS ML-DmBG1-c1		(85)
CNS ML-DmBG2-c2		(85)
tumorous blood cells mbn2		(39)
ovary fGS/OSS		(126)
ovary OSC		(94)
ovary OSS		(75)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(142)
Schneider line 2 Sg4		(121)
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		(134)
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		(110)
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		(126)
ovary OSC		94
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		142
Schneider line 2 Sg4		121
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		134
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		110
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		126
ovary OSC		94
ovary OSS		75
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		142
Schneider line 2 Sg4		121
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		134
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		110
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		126
ovary OSC		94
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(142)
Schneider line 2 Sg4		(121)
embryonic 1182-4H		(89)
embryonic GM2		(50)
embryonic Kc167		(43)
embryonic S1		(83)
embryonic S3		(134)
leg disc CME L1		(105)
wing disc CME-W2		(75)
wing disc ML-DmD8		(84)
wing disc ML-DmD9		(101)
wing disc ML-DmD16-c3		(102)
wing disc ML-DmD21		(110)
wing disc ML-DmD32		(50)
haltere disc ML-DmD17-c3		(105)
eye-antennal disc ML-DmD11		(62)
antennal disc ML-DmD20-c5		(89)
mixed discs ML-DmD4-c1		(92)
CNS ML-DmBG1-c1		(85)
CNS ML-DmBG2-c2		(85)
tumorous blood cells mbn2		(39)
ovary fGS/OSS		(126)
ovary OSC		(94)
ovary OSS		(75)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		142
Schneider line 2 Sg4		121
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		134
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		110
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		126
ovary OSC		94
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		142
Schneider line 2 Sg4		121
embryonic 1182-4H		89
embryonic GM2		50
embryonic Kc167		43
embryonic S1		83
embryonic S3		134
leg disc CME L1		105
wing disc CME-W2		75
wing disc ML-DmD8		84
wing disc ML-DmD9		101
wing disc ML-DmD16-c3		102
wing disc ML-DmD21		110
wing disc ML-DmD32		50
haltere disc ML-DmD17-c3		105
eye-antennal disc ML-DmD11		62
antennal disc ML-DmD20-c5		89
mixed discs ML-DmD4-c1		92
CNS ML-DmBG1-c1		85
CNS ML-DmBG2-c2		85
tumorous blood cells mbn2		39
ovary fGS/OSS		126
ovary OSC		94
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		(44)
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		(39)
Cadmium 100 mM 48 hrs, 4-day adult		(60)
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		(38)
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		(66)
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(53)
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		(48)
Paraquat 10 mM 48 hrs, 4-day adult		(44)
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		(44)
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		(39)
Cadmium 100 mM 48 hrs, 4-day adult		(60)
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		(38)
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		(66)
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(53)
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		(48)
Paraquat 10 mM 48 hrs, 4-day adult		(44)
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		44
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		26
Cadmium 50 mM 48 hrs, 4-day adult		39
Cadmium 100 mM 48 hrs, 4-day adult		60
Copper 0.5 mM 12 hrs, larvae L3		20
Copper 15 mM 48 hrs, 4-day adult		38
Zinc 5 mM 12 hrs, larvae L3		15
Zinc 4.5 mM 48 hrs, 4-day adult		16
Ethanol 2.5% 3 hrs, larvae L3		66
Ethanol 5% 3 hrs, larvae L3		24
Ethanol 10% 3 hrs, larvae L3		22
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		53
Caffeine 25 mg/ml 48 hrs, 4-day adult		25
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		44
Rotenone 2 μg 12 hrs, larvae L3		24
Rotenone 8 μg 12 hrs, larvae L3		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD22577

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0029113

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG7528-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Uba2

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

cell lethal | somatic clone | third instar larval stage

Uba2^N2

lethal (with Df(3L)Exel6112)

lethal | P-stage | recessive

Other Phenotypes

Allele

decreased cell number | somatic clone | third instar larval stage

Uba2^N2

immune response defective, with Scer\GAL4^Cg.PA

Uba2^HM05055

immune response defective, with Scer\GAL4^Hml.PG

Uba2^HM05055

immune response defective, with Scer\GAL4^Lsp2.PH

Uba2^HM05055

increased cell death | somatic clone | third instar larval stage

Uba2^N2

increased cell death | temperature conditional, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

tumorigenic, with Scer\GAL4^Cg.PA

Uba2^HM05055

tumorigenic, with Scer\GAL4^Hml.PG

Uba2^HM05055

tumorigenic, with Scer\GAL4^Lsp2.PH

Uba2^HM05055

visible, with Scer\GAL4^ey.PU

Uba2^NIG.7528R

visible, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

Phenotype manifest in

Allele

adult head, with Scer\GAL4^ey.PU

Uba2^NIG.7528R

embryonic/larval fat body, with Scer\GAL4^Cg.PA

Uba2^HM05055

embryonic/larval fat body, with Scer\GAL4^Hml.PG

Uba2^HM05055

embryonic/larval hemocoel, with Scer\GAL4^Cg.PA

Uba2^HM05055

embryonic/larval hemocoel, with Scer\GAL4^Hml.PG

Uba2^HM05055

embryonic/larval hemocoel, with Scer\GAL4^Lsp2.PH

Uba2^HM05055

eye, with Scer\GAL4^ey.PU

Uba2^NIG.7528R

eye, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

eye | temperature conditional, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

eye disc, with Scer\GAL4^ey.PU

Uba2^NIG.7528R

imaginal disc

ommatidium, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

ommatidium | temperature conditional, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

optic lobe

pigment cell, with Scer\GAL4^GMR.PF

Uba2^{C175S.Scer\UAS}

wing disc | somatic clone

Uba2^N2

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Uba2	Class	Mutagen	Known lesion
Uba2^C3	amorphic allele - molecular evidence	P-element activity	Yes
Uba2^N2	amorphic allele - molecular evidence	P-element activity	Yes
Uba2^G4384		P-element activity	--
Uba2^G6571		P-element activity	--
Uba2^G8583		P-element activity	--

Alleles Carried on Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Uba2	Mutagen	Stocks	Known lesion
Uba2^GL00452	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Uba2^HM05055	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Uba2^+t4.4	in vitro construct - genomic fragment	0	Yes
Uba2^{Act5C.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Uba2^{C175S.Scer\UAS}	in vitro construct - regulatory fusion	0	Yes
Uba2^GD12265	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Uba2^KK102220	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Uba2^NIG.7528R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Uba2^Scer\UAS.cKa	in vitro construct - regulatory fusion	0	Yes
Uba2^Scer\UAS.cLa	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)Exel6112

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{Uba2.+4.4}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{EP-ΔC3}Uba2^C3

P{EP-ΔN2}Uba2^N2

insertion of mobile activating element

P{EP}Uba2^G4384

P{EP}Uba2^G6571

P{EP}Uba2^G8583

Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )

Terms Based on Experimental Evidence ( 6 terms )

Molecular Function

CV term

Evidence

References

protein binding

inferred from physical interaction with Aos1 AND inferred from physical interaction with lwr

(Shih et al., 2002)

Biological Process

CV term

Evidence

References

humoral immune response

inferred from mutant phenotype

(Paddibhatla et al., 2010)

positive regulation of NF-kappaB transcription factor activity

inferred from direct assay

(Bhaskar et al., 2000)

protein sumoylation

inferred from mutant phenotype

(Kanakousaki and Gibson, 2012)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Shih et al., 2002)

nucleus

inferred from direct assay

(Shih et al., 2002, Smith et al., 2004)

Terms Based on Predictions or Assertions ( 7 terms )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000127

(FlyBase Curators et al., 2004-)

small protein activating enzyme activity

inferred from sequence or structural similarity

(FlyBase, 1992-)

non-traceable author statement

(Shih, 1999.10.8)

SUMO activating enzyme activity

inferred from sequence or structural similarity

(Bhaskar et al., 2000)

inferred from sequence or structural similarity with EMBL:AF090384

(FlyBase, 1992-)

non-traceable author statement

(Bhaskar, 1999.12.27)

ubiquitin activating enzyme activity

non-traceable author statement

(Shih et al., 1998)

Biological Process

CV term

Evidence

References

protein sumoylation

inferred from sequence or structural similarity

(Bhaskar et al., 2000)

SMT3-dependent protein catabolic process

non-traceable author statement

(Bhaskar, 1999.12.27)

Cellular Component

CV term

Evidence

References

SUMO activating enzyme complex

inferred from sequence or structural similarity

(Bhaskar et al., 2000)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Uba2 - Aos1

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Uba2 - CG7675

experimental knowledge based

(Guruharsha et al., 2011)

Uba2 - Ubc12

experimental knowledge based

(Guruharsha et al., 2011)

Uba2 - lwr

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Uba2 - p53

pull down, autoradiography, two hybrid

(Pardi et al., 2011)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Uba2 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

69084

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0029113

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

44496

Orthologs

OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6V43T8

Dipteran inclusive ortholog search

EOG6N30X5

Insect inclusive ortholog search

EOG67M0T1

Arthropod inclusive ortholog search

EOG6N5TC0

Metazoa inclusive ortholog search

EOG6KDKD4

Orthologs in Drosophila Species (EOG6V43T8)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20416

Drosophila persimilis

Dper\GL15364

Drosophila persimilis

Dper\GL22481

Drosophila willistoni

Dwil\GK19989

Drosophila virilis

Dvir\GJ12222

Drosophila mojavensis

Dmoj\GI11997

Drosophila grimshawi

Dgri\GH15287

Orthologs in non-Drosophila Dipterans (EOG6N30X5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010641

Anopheles gambiae

Malaria mosquito

Agam\AGAP008637

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007700

Orthologs in non-Dipteran Insects (EOG67M0T1)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\Uba2

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000213

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH22816

Atta cephalotes

Leafcutter ant

Acep\ACEP14202

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO16498

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL23560

Linepithema humile

Argentine ant

Lhum\LH19493

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB11236

Solenopsis invicta

Red fire ant

Sinv\SINV19650

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006138

Bombyx mori

Silkmoth

Bmor\BGIBMGA012116

Pediculus humanus

Human body louse

Phum\PHUM280660

Tribolium castaneum

Red flour beetle

Tcas\TC002294

Orthologs in non-Insect Arthropods (EOG6N5TC0)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\GDI1

Ixodes scapularis

Deer tick

Isca\ISCW007746

Orthologs in non-Arthropod Metazoa (EOG6KDKD4)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\uba-2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_021280gn

Danio rerio

Zebrafish

Drer\uba2

Xenopus tropicalis

Western clawed frog

Xtro\uba2

Gallus gallus

Domestic chicken

Ggal\SAE2

Mus musculus

House mouse

Mmus\Uba2

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000021113

Rattus norvegicus

Norway rat

Rnor\Uba2

Homo sapiens

Human

Hsap\UBA2

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\UBA2

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20416

Drosophila persimilis

Dper\GL22481

Drosophila willistoni

Dwil\GK19989

Drosophila virilis

Dvir\GJ12222

Drosophila mojavensis

Dmoj\GI11997

Drosophila grimshawi

Dgri\GH15287

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

Bloomington

35806

y¹ sc^* v¹; P{TRiP.GL00452}attP2

28569

y¹ v¹; P{TRiP.HM05055}attP2/TM3, Sb¹

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 158 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD22577

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0029113

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

44496

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Uba2 SAE2 CG7528

(Bayraktaroglu, 2000.5.25)

Additional comments

FlyBase curator comment: the symbol "sip2" has been used to refer to two genes; both the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) - in FBrf0108803, FBrf0102083 and FBrf0101408 and also the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878) - in FBrf0144953.

(Shih, 2003.6.25)

FlyBase curator comment: the gene referred to as "sip2" in FBrf0108803 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

(Hsin-Pei et al., 1998)

FlyBase curator comment: the gene referred to as "sip2" in FBrf0101408 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

(Shih et al., 1998)

FlyBase curator comment: the gene referred to as "sip2" in FBrf0102083 corresponds to the gene referred to in FlyBase as Uba2 (CG7528, FBgn0029113) and does NOT correspond to the gene referred to in FlyBase as sip2 (CG9188, FBgn0031878).

(Shih, 1998.3.17)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

Identification: Yeast two-hybrid screen for proteins that interact with the pnut, Sep1 or Sep2 proteins. Uba2 interacts with Sep1 and Sep2 in the two-hybrid system and does not interact with pnut.

(Shih et al., 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_080017

RefSeq (Proteins)

•

NP_524756

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

44496

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-activating enzyme, E1, active site (IPR018074)

Ubiquitin-activating enzyme repeat (IPR000127)

UBA/THIF-type NAD/FAD binding fold (IPR000594)

Molybdenum cofactor biosynthesis, MoeB (IPR009036)

Ubiquitin-activating enzyme (IPR019572)

NAD(P)-binding domain (IPR016040)

Ubiquitin-like 1 activating enzyme, catalytic cysteine domain (IPR023280)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD22577

Linkouts

BioGRID - A database of protein and genetic interactions

•

69084

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0029113

DRSC - Results from RNAi screens.

•

FBgn0029113

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0029113

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG7528-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0029113

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

44496

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

44496

modMine - Data generated by the modENCODE project.

•

FBgn0029113

Synonyms & Secondary IDs ( 17 )

Reported As

Symbol Synonym

CG7528

(Suzuki et al., 2001, Bayraktaroglu, 2000.5.25, Dorer et al., 2006, Perkins et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Vidal et al., 2010)

DmSae2

(Smith et al., 2001)

DmSAE2

(Bhaskar et al., 2000)

DmUba2

(Shih, 2003.6.25, Shih et al., 2002, Long and Griffith, 2000)

Dmuba2

(Shih et al., 2000)

dUba2

(Donaghue et al., 2001)

L36

(Suzuki et al., 2001)

SAE2

(Bhaskar et al., 2002, Smith et al., 2004, Bayraktaroglu, 2000.5.25, Bhaskar, 1999.12.27, Nie et al., 2009)

Sae2

(Smith et al., 2002)

sip2

(Shih, 2003.6.25, Hsin-Pei et al., 1998, Shih, 1998.3.17, Shih et al., 1998, )

Uba2

(Altenhein et al., 2006, Shigenobu et al., 2006, Chan et al., 2002, Hashiyama et al., 2009, Jacques et al., 2009, Zhang et al., 2010, Arya et al., 2010, Paddibhatla et al., 2010, Vidal et al., 2010, Takács et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kim et al., 2010)

uba2

(Pardi et al., 2011, Kim et al., 2010, Zhang et al., 2010, Kanakousaki and Gibson, 2012)

UBA2

(Dorer et al., 2006, Pardi et al., 2011)

Ubc2

(Toba and White, 2003)

Name Synonym

Smt3 activating enzyme 2

(Bhaskar, 1999.12.27)

Smt3-activating enzyme 2

(Kiss et al., 2005)

ubiquitin-activating enzyme (E1)

(Speese et al., 2003)

Secondary FlyBase IDs

FBgn0024190
FBgn0035840
FBgn0040287

References ( 55 )

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All references relating to this gene ( 55 )

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Research paper ( 27 )
Supplementary material ( 5 )
Personal communication to FlyBase ( 10 )
Abstract ( 6 )
FlyBase analysis ( 1 )
DNA/RNA sequence record ( 2 )
Stock list ( 1 )
Website ( 1 )
Curated genome annotation ( 1 )

Recent research papers ( 5 )

Kalamarz et al., 2012, Biol. Open 1(3): 161--172: Sumoylation is tumor-suppressive and confers proliferative quiescence to hematopoietic progenitors in Drosophila melanogaster larvae. [FBrf0220167]
Kanakousaki and Gibson, 2012, Development 139(15): 2751--2762: A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development. [FBrf0218825]
Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134: Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342]
Takács et al., 2012, Int. J. Biol. Sci. 8(7): 1055--1061: Protein interactions on telomeric retrotransposons in Drosophila. [FBrf0219367]
Pardi et al., 2011, Acta biol. hung. 62(4): 397--412: In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. [FBrf0216737]

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