FlyBase Gene Report: Dmel\Debcl

General Information

Symbol

Dmel\Debcl

Species

D. melanogaster

Name

Death executioner Bcl-2

Annotation Symbol

CG33134

Feature Type

protein_coding_gene

FlyBase ID

FBgn0029131

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Snapshot

Death executioner Bcl-2 (Debcl) encodes a pro-apoptotic member of the Bcl-2 family involved in programmed cell death. [Date last reviewed: 2019-07-11]

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Drob-1, dBorg-1, Bcl-2, dBok, dBorg1

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:6,648,654..6,652,842 [+]

Recombination map

2-55

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

BCL-2 FAMILY

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

protein heterodimerization activity; protein homodimerization activity

Summaries

Gene Group (FlyBase)

BCL-2 FAMILY -: The Bcl-2 family are a group of evolutionary-related genes sharing Bcl-2 homology (BH) domains. Bcl-2 proteins can have pro- or anti-apoptotic activities. (Adapted from FBrf0187831).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Debcl or the JBrowse view of Dmel\Debcl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Debcl-RA

FBtr0086108

NM_176098

1724

300

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Colussi et al., 2000)

1.3 (northern blot)

(Igaki et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Debcl-PA

FBpp0085443

32.9

300

9.53

NP_788278

AAF57365

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

300 (aa); 33 (kD predicted)

(Colussi et al., 2000)

325 (aa); 35 (kD predicted)

(Igaki et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Debcl using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Debcl_1

Linkouts

Gene Ontology (21 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

protein heterodimerization activity

inferred from biological aspect of ancestor with PANTHER:PTN000135648

(assigned by GO_Central )

(Gaudet et al., 2011)

protein homodimerization activity

inferred from biological aspect of ancestor with PANTHER:PTN000135648

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (16 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

apoptotic process

inferred from mutant phenotype

(Brachmann et al., 2000)

inferred from genetic interaction with FLYBASE:Diap1; FB:FBgn0260635

(Colussi et al., 2000)

inferred from genetic interaction with FLYBASE:Dark; FB:FBgn0263864

(Colussi et al., 2000)

apoptotic process involved in morphogenesis

inferred from mutant phenotype

(Colussi et al., 2000)

NOT autophagy

inferred from mutant phenotype

(Galindo et al., 2009)

cellular response to starvation

inferred from mutant phenotype

(Hou et al., 2008)

ectopic germ cell programmed cell death

inferred from mutant phenotype

(Yacobi-Sharon et al., 2013)

intrinsic apoptotic signaling pathway in response to DNA damage

inferred from mutant phenotype

(Brachmann et al., 2000)

negative regulation of neuron apoptotic process

inferred from mutant phenotype

(Senoo-Matsuda et al., 2005)

neuron cellular homeostasis

inferred from genetic interaction with FLYBASE:Ank2; FB:FBgn0261788

(Keller et al., 2011)

positive regulation of macroautophagy

inferred from mutant phenotype

(Hou et al., 2008)

positive regulation of necrotic cell death

inferred from mutant phenotype

(Napoletano et al., 2017)

programmed cell death involved in cell development

inferred from mutant phenotype

(Galindo et al., 2009)

regulation of programmed cell death

inferred from genetic interaction with FLYBASE:Dark; FB:FBgn0263864

(Galindo et al., 2009)

response to ionizing radiation

inferred from mutant phenotype

(Galindo et al., 2009)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

apoptotic process

traceable author statement

(Gorski and Marra, 2002, Richardson and Kumar, 2002, Tittel and Steller, 2000)

non-traceable author statement

(Lawen, 2003)

inferred from sequence or structural similarity with HGNC:990

(Igaki et al., 2000)

extrinsic apoptotic signaling pathway in absence of ligand

inferred from biological aspect of ancestor with PANTHER:PTN000135648

(assigned by GO_Central )

(Gaudet et al., 2011)

intrinsic apoptotic signaling pathway in response to DNA damage

inferred from biological aspect of ancestor with PANTHER:PTN000135648

(assigned by GO_Central )

(Gaudet et al., 2011)

negative regulation of apoptotic process

inferred from sequence or structural similarity with UniProtKB:P10415

(Sardiello et al., 2003.6.11)

regulation of mitochondrial membrane permeability

inferred from sequence or structural similarity with UniProtKB:P10415

(Sardiello et al., 2003.6.11)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

mitochondrial envelope

inferred from direct assay

(Igaki et al., 2000)

mitochondrion

inferred from direct assay

(Doumanis et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

mitochondrial outer membrane

inferred from biological aspect of ancestor with PANTHER:PTN000135648

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:P10415

(Sardiello et al., 2003.6.11)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism | ubiquitous

(Igaki et al., 2000)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11

posterior midgut primordium

(Colussi et al., 2000)

mesoderm

(Brachmann et al., 2000)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

visceral muscle primordium

(Fisher et al., 2012)

embryonic stage 13

embryonic/larval gut

(Brachmann et al., 2000)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval visceral muscle

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

third instar larval stage

brain | restricted

(Colussi et al., 2000)

eye-antennal disc | restricted

(Colussi et al., 2000)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Colussi et al., 2000)

embryonic stage 5

organism | ubiquitous

(Colussi et al., 2000)

embryonic stage 11

proctodeum

(Colussi et al., 2000)

embryonic stage 14 -- 16

embryonic/larval midgut

(Colussi et al., 2000)

embryonic head segment | restricted

(Colussi et al., 2000)

third instar larval stage

embryonic/larval salivary gland duct

(Colussi et al., 2000)

adult stage & oogenesis

nurse cell

(Colussi et al., 2000)

oocyte

(Colussi et al., 2000)

pcr

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Igaki et al., 2000)

third instar larval stage

wing disc

(Igaki et al., 2000)

eye disc

(Igaki et al., 2000)

embryonic/larval midgut

(Igaki et al., 2000)

ventral thoracic disc

(Igaki et al., 2000)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Brachmann et al., 2000)

adult stage | male

head

(Brachmann et al., 2000)

abdomen & thorax

(Brachmann et al., 2000)

adult stage | female

head

(Brachmann et al., 2000)

abdomen & thorax

(Brachmann et al., 2000)

Additional Descriptive Data

RT-PCR analysis indicates that Debcl transcripts are expressed at all stages of development. In situ hybridization reveals that Debcl transcripts are expressed at high levels in early embryos. Expression is also observed in third instar larvae in leg, wing, and eye disc and in the midgut.

(Igaki et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

mitochondrial envelope

inferred from direct assay

(Igaki et al., 2000)

mitochondrion

inferred from direct assay

(Doumanis et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Debcl in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Debcl

View exonic expression by tissue for Dmel\Debcl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029131

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029131

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33134-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33134

Flygut - An atlas of the Drosophila adult midgut

FBgn0029131

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Debcl

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Debcl

Transgenic constructs containing regulatory region of Debcl

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)hfl-37

(Rendina et al., 2010)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

Debcl^dsRNA.UAS

partially lethal - majority die | larval stage, with Scer\GAL4^elav-C155

Debcl^dsRNA.UAS

partially lethal - majority die | pupal stage, with Scer\GAL4^elav-C155

Debcl^dsRNA.UAS

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Debcl^GD1637

viable, with Scer\GAL4^Mef2.PR

Debcl^GD1637

viable, with Scer\GAL4^pnr-MD237

Debcl^GD1637

viable, with Scer\GAL4^tin.CΔ4

Debcl^GD1637

viable (with Debcl⁶)

Debcl^{KO-unspecified}

viable (with Debcl²²)

Debcl^{KO-unspecified}

viable (with Debcl²³)

Debcl^{KO-unspecified}

viable (with Debcl²⁷)

Debcl^{KO-unspecified}

viable (with Debcl⁵⁹)

Debcl^{KO-unspecified}

viable (with Debcl⁶¹)

Debcl^{KO-unspecified}

viable (with Debcl^{KO-unspecified})

Sterility

Allele

fertile

fertile (with Debcl⁶)

Debcl^{KO-unspecified}

fertile (with Debcl²²)

Debcl^{KO-unspecified}

fertile (with Debcl²³)

Debcl^{KO-unspecified}

fertile (with Debcl²⁷)

Debcl^{KO-unspecified}

fertile (with Debcl⁵⁹)

Debcl^{KO-unspecified}

fertile (with Debcl⁶¹)

Debcl^{KO-unspecified}

fertile (with Debcl^{KO-unspecified})

Other Phenotypes

Allele

decreased cell death | embryonic stage

decreased cell death | embryonic stage, with Scer\GAL4^da.G32

Debcl^dsRNA.UAS

decreased cell death | embryonic stage (with Debcl²²)

Debcl²⁷

decreased cell death | embryonic stage (with Debcl²⁷)

Debcl²²

decreased cell death | embryonic stage (with Debcl⁵⁹)

Debcl⁶¹

decreased cell death | embryonic stage (with Debcl⁶¹)

Debcl⁵⁹

decreased cell death | spermatogenesis

Debcl²⁷

hyperplasia | adult stage

Debcl²⁷

increased cell death, with Scer\GAL4^109-88

Debcl^UAS.Tag:HA

increased cell death, with Scer\GAL4^ey.PH

Debcl^UAS.Tag:HA

increased cell death, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

increased cell death, with Scer\GAL4^hs.PB

Debcl^UAS.Tag:HA

increased cell death | third instar larval stage, with Scer\GAL4^ptc.PU

Debcl^UAS.Tag:HA

locomotor behavior defective, with Scer\GAL4^elav-C155

Debcl^dsRNA.UAS

locomotor behavior defective | progressive, with Scer\GAL4^Ddc.PL

Debcl^UAS.Tag:HA

long lived, with Scer\GAL4^elav-C155

Debcl^GS2263

neuroanatomy defective | somatic clone, with Scer\GAL4^ppk.1.9

Debcl^UAS.Tag:HA

radiation sensitive

radiation sensitive (with Debcl²²)

radiation sensitive (with Debcl²⁷)

radiation sensitive (with Debcl⁴⁷)

Debcl⁴⁸

radiation sensitive (with Debcl⁴⁸)

Debcl⁴⁷

radiation sensitive (with Debcl⁵⁹)

radiation sensitive (with Debcl⁶¹)

short lived, with Scer\GAL4^Ddc.PL

Debcl^UAS.Tag:HA

short lived, with Scer\GAL4^elav-C155

Debcl^dsRNA.UAS

size defective | adult stage, with Scer\GAL4^GMR.PU

Debcl^UAS.cUa

some die during larval stage, with Scer\GAL4^da.G32

Debcl^dsRNA.UAS

visible

Debcl^GMR.PI

visible, with Scer\GAL4^GMR.PF

visible | adult stage, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

visible | adult stage, with Scer\GAL4^GMR.PU

Debcl^UAS.cUa

visible | adult stage, with Scer\GAL4^ptc.PU

Debcl^UAS.Tag:HA

Phenotype manifest in

Allele

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

Debcl^UAS.Tag:HA

Bolwig organ | embryonic stage

dendrite & abdominal anterior ventral multidendritic neuron vdaa, with Scer\GAL4^ppk.1.9

Debcl^UAS.Tag:HA

dendrite & class IV dendritic arborising neuron, with Scer\GAL4^ppk.1.9

Debcl^UAS.Tag:HA

dendrite & dorsal multidendritic neuron ddaC | somatic clone, with Scer\GAL4^ppk.1.9

Debcl^UAS.Tag:HA

eye

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

Debcl^UAS.cUa

eye | adult stage, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

eye disc, with Scer\GAL4^ey.PH

Debcl^UAS.Tag:HA

eye disc, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

eye disc | posterior, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

eye photoreceptor cell

Debcl^GMR.PI

interommatidial bristle

Debcl^GMR.PB

male genitalia

Debcl^{KO-unspecified}

maxillary palpus

Debcl^{KO-unspecified}

melanotic mass

Debcl^{KO-unspecified}

midline glial cell | embryonic stage | supernumerary

Debcl⁵⁹

mitochondrion & dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

Debcl^UAS.Tag:HA

ommatidium

Debcl^GMR.PI

ommatidium | adult stage, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

pigment cell

Debcl^GMR.PB

pigment cell, with Scer\GAL4^GMR.PF

Debcl^UAS.Tag:HA

salivary gland, with Scer\GAL4^109-88

Debcl^UAS.Tag:HA

scutellar bristle | supernumerary

spermatogonium

Debcl²⁷

sternite

Debcl^{KO-unspecified}

tergite

Debcl^{KO-unspecified}

testis | adult stage

Debcl²⁷

vagina

Debcl^{KO-unspecified}

ventral nerve cord | embryonic stage (with Debcl²²)

Debcl²⁷

ventral nerve cord | embryonic stage (with Debcl²⁷)

Debcl²²

ventral nerve cord | embryonic stage (with Debcl⁵⁹)

Debcl⁶¹

ventral nerve cord | embryonic stage (with Debcl⁶¹)

Debcl⁵⁹

wing, with Scer\GAL4^en-e16E

Debcl^UAS.Tag:HA

wing | adult stage, with Scer\GAL4^ptc.PU

Debcl^UAS.Tag:HA

wing margin | conditional (with Debcl²²)

wing margin | conditional (with Debcl²⁷)

wing margin | conditional (with Debcl⁴⁷)

Debcl⁴⁸

wing margin | conditional (with Debcl⁴⁸)

Debcl⁴⁷

wing margin | conditional (with Debcl⁵⁹)

wing margin | conditional (with Debcl⁶¹)

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\BOK

8 of 15

Yes

Hsap\BAK1

1 of 15

Yes

Hsap\BCL2A1

1 of 15

Yes

Hsap\BCL2

1 of 15

Yes

Hsap\BCL2L1

1 of 15

Yes

Hsap\BCL2L2-PABPN1

1 of 15

Hsap\BCL2L14

1 of 15

Yes

Hsap\MCL1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Bok

8 of 15

Yes

Mmus\Bak1

1 of 15

Yes

Mmus\Bcl2a1a

1 of 15

Yes

Mmus\Bcl2a1b

1 of 15

Yes

Mmus\Bcl2a1c

1 of 15

Yes

Mmus\Bcl2a1d

1 of 15

Yes

Mmus\Bcl2l1

1 of 15

Yes

Mmus\Bcl2l14

1 of 15

Yes

Mmus\Bcl2

1 of 15

Yes

Mmus\Mcl1

1 of 15

Yes

Mmus\Pabpn1

1 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Bok

8 of 13

Yes

Rnor\Bak1

1 of 13

Yes

Rnor\Bcl2a1

1 of 13

Yes

Rnor\Bcl2l1

1 of 13

Yes

Rnor\Bcl2l14

1 of 13

Yes

Rnor\Bcl2

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (5)

Xtro\bok

3 of 12

Yes

Xtro\bcl2l1

1 of 12

Yes

Xtro\bcl2l2

1 of 12

Yes

Xtro\bcl2

1 of 12

Yes

Xtro\mcl1

1 of 12

Yes

Danio rerio (Zebrafish) (7)

Drer\boka

8 of 15

Yes

Drer\bokb

7 of 15

Yes

Drer\bcl2a

1 of 15

Yes

Drer\bcl2b

1 of 15

Drer\bcl2l1

1 of 15

Yes

Drer\mcl1a

1 of 15

Yes

Drer\mcl1b

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\ced-9

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190DGO )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Debcl

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17309

Drosophila persimilis

Dper\GL20359

Drosophila willistoni

Dwil\GK19537

Drosophila virilis

Dvir\GJ21634

Drosophila mojavensis

Dmoj\GI18625

Drosophila grimshawi

Dgri\GH21025

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A04 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17013686

Musca domestica

House fly

Mdoa\17013684

Musca domestica

House fly

Mdoa\17006851

Glossina morsitans

Tsetse fly

Gmor\GMOY000585

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_06601

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04459

Mayetiola destructor

Hessian fly

Mdes\Mdes006991

Mayetiola destructor

Hessian fly

Mdes\Mdes006989

Aedes aegypti

Yellow fever mosquito

Aaeg\BUFFY

Aedes aegypti

Yellow fever mosquito

Aaeg\DEBCL

Anopheles darlingi

American malaria mosquito

Adar\ADAC001493

Anopheles darlingi

American malaria mosquito

Adar\ADAC001492

Anopheles darlingi

American malaria mosquito

Adar\ADAC002207

Anopheles gambiae

Malaria mosquito

Agam\anob-1

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002201

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0D75 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Danaus plexippus

Monarch butterfly

Dple\DPOGS215828-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011638

Apis florea

Little honeybee

Aflo\2332180756

Apis mellifera

Western honey bee

Amel\LOC411613

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11724

Bombus terrestris

Buff-tailed bumblebee

Bter\15204022

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00286a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006942

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06106

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06107

Tribolium castaneum

Red flour beetle

Tcas\TC013830

Pediculus humanus

Human body louse

Phum\PHUM582920

Rhodnius prolixus

Kissing bug

Rpro\RPRC008771

Cimex lectularius

Bed bug

Clec\CLEC005509

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005643

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_16931

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0D4Z )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005159

Ixodes scapularis

Black-legged tick

Isca\ISCW019088

Ixodes scapularis

Black-legged tick

Isca\ISCW008937

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81480.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74886.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur19g02820

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0JPB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Boc

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Bokl

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007880

Ciona intestinalis

Vase tunicate

Cint\LOC100185339

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000005772

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

6 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Debcl²⁷

model of testicular cancer

CEA with Dronc^L32

(Napoletano et al., 2017)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Debcl^UAS.Tag:HA

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(M'Angale and Staveley, 2016)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Senoo-Matsuda et al., 2005)

Debcl^dsRNA.UAS

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Senoo-Matsuda et al., 2005)

Debcl^JF02429

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(M'Angale and Staveley, 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BOK; BCL2 family apoptosis regulator BOK

8 of 15

605404

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Debcl - Buffy

anti tag coimmunoprecipitation, anti tag western blot

(Nakazawa et al., 2016, Quinn et al., 2003)

Debcl - Drp1

anti tag coimmunoprecipitation, anti tag western blot

(Clavier et al., 2015)

Debcl - grim

anti tag coimmunoprecipitation, anti tag western blot

(Wu et al., 2010)

Debcl - slmb

anti tag coimmunoprecipitation, anti tag western blot, proximity ligation assay, fluorescence microscopy

(Colin et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Debcl

suppressible

Buffy

(M'Angale and Staveley, 2016, Tsubouchi et al., 2009, Quinn et al., 2003)

Debcl

suppressible

Dark

(Colussi et al., 2000)

Debcl

enhanceable

Prosα2

(Colin et al., 2014)

Debcl

suppressible

Rpn10

(Colin et al., 2014)

Debcl

enhanceable

slmb

(Colin et al., 2014)

Debcl

suppressible

Uba1

(Colin et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ank2

suppressible

Debcl

(Keller et al., 2011)

bsk|mad2

suppressible

Debcl

(Wong et al., 2014)

Dark

enhanceable

Debcl

(Galindo et al., 2009)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72808

DroID - A comprehensive database of gene and protein interactions.

FBgn0029131

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53585

MIST (genetic) - An integrated Molecular Interaction Database

Debcl

MIST (protein-protein) - An integrated Molecular Interaction Database

Debcl

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42B2-42C1

Sequence location

2R:6,648,654..6,652,842 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42B2-42C1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42B-42B

(Zhou, 2000.1.6)

42E-43A

(Brachmann, 2000.3.10, Colussi, 1999.8.17, Kurada, 1999.12.14)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Bloomington

58357

w^*; P{UAS-Debcl.HA}26

27342

w¹¹¹⁸; Debcl^E26

27341

w¹¹¹⁸; Debcl^W105

27083

y¹ v¹; P{TRiP.JF02429}attP2

81812

y¹ v¹; P{TKO.GS04860}attP40/CyO

e00816

f04864

v106669

v47517

w¹¹¹⁸ P{GD1637}v47517

v47515

w¹¹¹⁸; P{GD1637}v47515

v47518

w¹¹¹⁸; P{GD1637}v47518

v47516

w¹¹¹⁸; P{GD1637}v47516/CyO

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (33)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

DPiM_IP01389

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029131

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029131

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53585

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Debcl debcl

(Steller et al., 2014.7.9)

Source for database merge of

Source for merge of: debcl BOK

(Bayraktaroglu, 2000.5.24)

Source for merge of: debcl CG12397

(FlyBase, 1996-)

Additional comments

Capitalized initial letter of gene symbol and name to be consistent with similarly named 'death genes' and to follow FlyBase nomenclature rules.

(Steller et al., 2014.7.9)

Source for merge of debcl CG12397 was sequence comparison ( date:000815 ).

(FlyBase Genome Annotators, 2002-2003, FlyBase, 1996-)

Other Comments

Gene expression is increased in response to the presence of two copies of Scer\GAL4^hs.PB.

(Liu and Lehmann, 2008)

debcl is not essential for normal development.

(Sevrioukov et al., 2007)

debcl protects neurons from neurodegeneration through the regulation of mitochondrial homeostasis.

(Senoo-Matsuda et al., 2005)

dsRNA made from templates generated with primers directed against this gene causes a phenotype in S2 cells. The reduced mitochondrial membrane potential and increase in cell death caused by treatment of cells with rotenone or 3-NP is enhanced. Cell death induction by treatment with proteasome inhibitors is likewise enhanced, while cell death induction by tunicamycin is not affected. Additionally, debcl knockdown significantly increases the percentage of mitochondria showing abnormal morphologies in S2 cells with intact nuclei and enhances the reduction of activities of mitochondrial respiratory chain complex I +III (NADH-cytochrome c oxidoreductase) and complex II +III (succinate-cytochrome c oxidoreductase) in S2 cells treated with rotenone or 3-NP.

(Senoo-Matsuda et al., 2005)

Area matching Drosophila ESTs AI062455 and AA440915.

(Davies, 2001.3.15)

debcl both induces apoptosis and demonstrates protective activity when death stimuli are introduced.

(Brachmann et al., 2000)

debcl encodes a pro-apoptotic protein that functions in a caspase-dependent manner. debcl is required for developmental apoptosis in the embryo.

(Colussi et al., 2000)

debcl appears to promote cell death by inducing both caspase-dependent and independent pathways at the mitochondria.

(Igaki et al., 2000)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53585

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029131

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029131

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Debcl_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33134

Flygut - An atlas of the Drosophila adult midgut

FBgn0029131

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53585

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG33134

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Apoptosis

modMine - A data warehouse for the modENCODE project

FBgn0029131

Linkouts

BioGRID - A database of protein and genetic interactions.

72808

DPiM - Drosophila Protein interaction map

FBgn0029131

DroID - A comprehensive database of gene and protein interactions.

FBgn0029131

DRSC - Results frm RNAi screens

FBgn0029131

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029131

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG33134-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0029131

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53585

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Debcl

MIST (protein-protein) - An integrated Molecular Interaction Database

Debcl

Synonyms and Secondary IDs (42)

Reported As

Symbol Synonym

BG1

(Zhou, 2000.1.6)

BOK

(Bayraktaroglu, 2000.5.24)

Bcl-2

(Zhang et al., 2015, Keller et al., 2011)

Bcl2

(Xu et al., 2018)

Bok

(Tittel and Steller, 2000)

Bok(Debcl)

(Lanave et al., 2004)

CG12397

(Brody et al., 2002, Richardson, 2001.4.10, Brody, 2000.9.6)

CG33134

(Keleman et al., 2009.8.5, Perkins et al., 2009.3.31, Wagner et al., 2009, Carrera et al., 2008)

DBok

(Sevrioukov et al., 2007, Zhang et al., 2000)

DEBCL

(Doumanis et al., 2007, Buszczak and Cooley, 2000)

Dbok

(Gorski and Marra, 2002, Zhang et al., 2000)

Debcl/Drob-1/dBorg-1/dBok

(Danial and Korsmeyer, 2004)

Debcl/dBorg-1/dBok/Drob-1

(Galindo and Abrams, 2007)

Debcl/dBorg-1/dRob-1

(Richardson and Kumar, 2002)

Drob-1

(Nakazawa et al., 2016, Kang and Ryoo, 2009, Sevrioukov et al., 2007, Kornbluth and White, 2005, Senoo-Matsuda et al., 2005, Senoo-Matsuda et al., 2004, Gorski and Marra, 2002, Igaki et al., 2002, Chen and Abrams, 2000, Igaki et al., 2000, Igaki, 1999.9.14)

Drob-1/Debcl/dBORG-1

(Baehrecke, 2000)

Drob-1/Debcl/dBorg-1/DBok

(Vernooy et al., 2000)

Drob-1/Debcl/dBorg-1/dBok

(Igaki and Miura, 2004)

bcl-2

(Wong et al., 2014, Brun et al., 2002)

borg1/debcl

(Ortega et al., 2005)

buffy

(Kuranaga, 2011)

dBOK

(Van Hoof and Goris, 2003)

dBORG-1/DROB-1/DEBCL/dBOK

(Meier et al., 2000)

dBok

(Tsubouchi et al., 2009, Kornbluth and White, 2005)

dBorg-1

(Kornbluth and White, 2005, Brachmann and Cagan, 2001, Brachmann et al., 2000, Vernooy et al., 2000)

dBorg01

(Gorski and Marra, 2002)

dBorg1

(Sevrioukov et al., 2007, Jassim et al., 2001)

dBrog-1

(Tsubouchi et al., 2009)

dbok

(Vernooy et al., 2000)

dbok/debcl

(Brun et al., 2002)

dborg1

(Brachmann, 2000.3.10)

debc1

(Blanco et al., 2010)

debcl/Borg1

(Burr et al., 2005, Purves et al., 2005)

debok

(Brun et al., 2001)

drob-1

(Tsubouchi et al., 2009, Brun et al., 2001, Vernooy et al., 2000)

Name Synonyms

Bcl-2

(Keller et al., 2011)

Death executioner Bcl-2

Death executioner Bcl-2 homologue

(Steller et al., 2014.7.9)

death executioner Bcl-2 homologue

(Colussi et al., 2000)

death inducing Bcl-2-like protein

(Colussi et al., 2000)

deborg

(Brun et al., 2001)

Secondary FlyBase IDs

FBgn0029509
FBgn0033080
FBgn0053134

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (145)

Report Sections

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