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Gene Dmel\Prosβ5

General Information
Symbol	Dmel\Prosβ5	Species	D. melanogaster
Name	Proteasome β5 subunit	Annotation symbol	CG12323
Feature type	protein_coding_gene	FlyBase ID	FBgn0029134
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Prosbeta5, dβ5, beta-5
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	47C1-47C1	Sequence location	2R:6,707,483..6,708,683 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Proteasome β5 subunit is referred to in FlyBase by the symbol Dmel\Prosβ5 (CG12323, FBgn0029134). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: endopeptidase activity. There is experimental evidence that it is involved in the biological process: centrosome organization; mitotic spindle elongation; cell proliferation; mitotic spindle organization; neurogenesis. 9 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Peptidase T1A, proteasome beta-subunit; Proteasome B-type subunit; Proteasome, beta-type subunit, conserved site; Proteasome, subunit alpha/beta. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1636559_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Prosbeta5. Gene sequence location is 2R:6707483..6708683.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47C1-47C1   Limits computationally determined from genome sequence between P{lacW}l(2)k00909k00909&P{EP}EP2619EP2619 and P{lacW}l(2)k15826k15826&P{EP}EP471
Experimentally Determined Cytological Location
Cytogenetic map Notes References 47B-47C     (Renault, 1999.12.20)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Prosβ5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Prosβ5-RA FBtr0088225  NM_143757   1139   282 Prosβ5-RB FBtr0088224  NM_165801   1076   282
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Prosβ5-PA   FBpp0087320   31.2   282   6.94     NP_652014   AAF58748 Prosβ5-PB   FBpp0087319   31.2   282   6.94     NP_724974   AAM68751
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase T1A, proteasome beta-subunit (IPR000243) Proteasome, subunit alpha/beta (IPR001353) Proteasome, beta-type subunit, conserved site (IPR016050) Proteasome B-type subunit (IPR023333)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263288   AA263715   AA390473   AA696596   AA950314   AE013599 AAF58748   AE013599 AAM68751   AF217726 AAF26416   AI063481   AI064230   AI107632   AI109084   AI259454   AI295050   AW941690   AX093992   AY069381 AAL39526   AY231648   AY232113   BG637055   BI142030   BI169377   BI169712   BI172187   BI242457   BI243880   BI355302   BI357336   BI366802   BI372505   BI372724   BI372803   BI372804   BI373294   BI374456   BI481985   BI483374   BI565704   BI569781   BI569997   BI575689   BI576615   BI576670   BI577480   BI588396   BI590095   BI591655   BI591714   BI609632   BI609825   BI611250   BI615177   BI615816   BI628278   BI631950   BP540970   BP545178   BP549392   BP558471   BX036626   BX044957   BX047802   CO181199   CO267024   CO280448   CO293840   CZ486175   CZ486176   EC210315   EC247238   EV577863   GH739637   GH740422   GH741384   GH742335   GH742758   GH744649   GH745325   GH745596   GH745854   GH747267   GH761534   GH762497   GH762569   GH763069   GH763375   GH763489   GH763533   GH764663   GH765578   GH765590   GH766510   GH766923   GH767003   GH767035   GH767622   GH768216   GH768747   GH769325   GH779185   GH779202   GH779225   GH779479   GH779607   GH779626   GH779656   GH779685   GH779719   GH779826   GH779861
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K148 Q9NHX7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
proteasome core complex inferred from direct assay (Sumegi et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1030.775 Larval Midgut    1111 Larval Hindgut    1063.8 Larval Malpighian Tubules    1798.8 Larval Fat Body    988.8 Larval Salivary Gland    1418.7 Larval Trachea    1384.675 Larval Carcass    1742.5 Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body    1634.2 Adult Salivary Gland    669.6 Adult Heart    2388.15 Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary    1220.3 Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass    1650.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1030.775) Larval Midgut  (1111) Larval Hindgut  (1063.8) Larval Malpighian Tubules  (1798.8) Larval Fat Body  (988.8) Larval Salivary Gland  (1418.7) Larval Trachea  (1384.675) Larval Carcass  (1742.5) Adult Head  (825.8) Adult Eye  (769.625) Adult Brain  (555.1) Adult Thoracic-Abdominal Ganglion  535.3 Adult Crop  (899) Adult Midgut  (853.8) Adult Hindgut  (761.7) Adult Malpighian Tubules  (947.7) Adult Fat Body  (1634.2) Adult Salivary Gland  (669.6) Adult Heart  (2388.15) Adult VirginFemale Spermatheca  (584.8) Adult InseminatedFemale Spermatheca  (607.6) Adult Ovary  (1220.3) Adult Testis    275.9 Adult Male Accessory Gland  (919.8) Adult Carcass  (1650.4) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1030.775 Larval Midgut  (1111) Larval Hindgut  1063.8 Larval Malpighian Tubules  (1798.8) Larval Fat Body    988.8 Larval Salivary Gland  (1418.7) Larval Trachea  (1384.675) Larval Carcass  (1742.5) Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body  (1634.2) Adult Salivary Gland    669.6 Adult Heart  (2388.15) Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary  (1220.3) Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass  (1650.4) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1030.775 Larval Midgut    1111 Larval Hindgut    1063.8 Larval Malpighian Tubules    1798.8 Larval Fat Body    988.8 Larval Salivary Gland    1418.7 Larval Trachea    1384.675 Larval Carcass    1742.5 Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body    1634.2 Adult Salivary Gland    669.6 Adult Heart    2388.15 Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary    1220.3 Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass    1650.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1030.775 Larval Midgut    1111 Larval Hindgut    1063.8 Larval Malpighian Tubules    1798.8 Larval Fat Body    988.8 Larval Salivary Gland    1418.7 Larval Trachea    1384.675 Larval Carcass    1742.5 Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body    1634.2 Adult Salivary Gland    669.6 Adult Heart    2388.15 Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary    1220.3 Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass    1650.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1030.775) Larval Midgut  (1111) Larval Hindgut  (1063.8) Larval Malpighian Tubules  (1798.8) Larval Fat Body  (988.8) Larval Salivary Gland  (1418.7) Larval Trachea  (1384.675) Larval Carcass  (1742.5) Adult Head  (825.8) Adult Eye  769.625 Adult Brain  555.1 Adult Thoracic-Abdominal Ganglion  535.3 Adult Crop  (899) Adult Midgut  (853.8) Adult Hindgut  761.7 Adult Malpighian Tubules  (947.7) Adult Fat Body  (1634.2) Adult Salivary Gland  669.6 Adult Heart  (2388.15) Adult VirginFemale Spermatheca  584.8 Adult InseminatedFemale Spermatheca  607.6 Adult Ovary  (1220.3) Adult Testis    275.9 Adult Male Accessory Gland  (919.8) Adult Carcass  (1650.4) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1030.775 Larval Midgut  1111 Larval Hindgut  1063.8 Larval Malpighian Tubules  (1798.8) Larval Fat Body    988.8 Larval Salivary Gland  1418.7 Larval Trachea  1384.675 Larval Carcass  (1742.5) Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body  1634.2 Adult Salivary Gland    669.6 Adult Heart  (2388.15) Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary  1220.3 Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass  1650.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1030.775 Larval Midgut    1111 Larval Hindgut    1063.8 Larval Malpighian Tubules    1798.8 Larval Fat Body    988.8 Larval Salivary Gland    1418.7 Larval Trachea    1384.675 Larval Carcass    1742.5 Adult Head    825.8 Adult Eye    769.625 Adult Brain    555.1 Adult Thoracic-Abdominal Ganglion    535.3 Adult Crop    899 Adult Midgut    853.8 Adult Hindgut    761.7 Adult Malpighian Tubules    947.7 Adult Fat Body    1634.2 Adult Salivary Gland    669.6 Adult Heart    2388.15 Adult VirginFemale Spermatheca    584.8 Adult InseminatedFemale Spermatheca    607.6 Adult Ovary    1220.3 Adult Testis    275.9 Adult Male Accessory Gland    919.8 Adult Carcass    1650.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    825.8   NA  Eye    769.625   NA  Brain    555.1   1030.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    535.3   NA  Crop    899   1111  Midgut    853.8   1063.8  Hindgut    761.7   1798.8  Malpighian Tubules    947.7   988.8  Fat Body    1634.2   1418.7  Salivary Gland    669.6   NA  Heart    2388.15   1384.675  Trachea    NA   NA  VirginFemale Spermatheca    584.8   NA  InseminatedFemale Spermatheca    607.6   NA  Ovary    1220.3   NA  Testis    275.9   NA  Male Accessory Gland    919.8   1742.5  Carcass    1650.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering    138 digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering    157 carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female    112 ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (73) central nervous system, larvae L3  (73) central nervous system, pupae P8  (32) head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female  (32) head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male  (31) head, mated 4-day male  (39) head, mated 20-day male  (62) salivary gland, larvae L3 wandering  (32) salivary gland, white prepupae  (36) digestive system, larvae L3 wandering  (138) digestive system, 1-day adult  (60) digestive system, 4-day adult  (46) digestive system, 20-day adult  (50) fat body, larvae L3 wandering    21 fat body, white prepupae  (56) fat body, pupae P8    25 carcass, larvae L3 wandering  (157) carcass, 1-day adult  (41) carcass, 4-day adult  (50) carcass, 20-day adult  (53) ovary, virgin 4-day female  (112) ovary, mated 4-day female  (93) testis, mated 4-day male  (69) accessory gland, mated 4-day male  (50) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering  (138) digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering  (157) carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female  (112) ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering    138 digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering    157 carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female    112 ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering    138 digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering    157 carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female    112 ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (73) central nervous system, larvae L3  (73) central nervous system, pupae P8  32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female  32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male  31 head, mated 4-day male  (39) head, mated 20-day male  (62) salivary gland, larvae L3 wandering  32 salivary gland, white prepupae  (36) digestive system, larvae L3 wandering  (138) digestive system, 1-day adult  (60) digestive system, 4-day adult  (46) digestive system, 20-day adult  (50) fat body, larvae L3 wandering    21 fat body, white prepupae  (56) fat body, pupae P8    25 carcass, larvae L3 wandering  (157) carcass, 1-day adult  (41) carcass, 4-day adult  (50) carcass, 20-day adult  (53) ovary, virgin 4-day female  (112) ovary, mated 4-day female  (93) testis, mated 4-day male  (69) accessory gland, mated 4-day male  (50) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering  138 digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering  (157) carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female  112 ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    73 central nervous system, larvae L3    73 central nervous system, pupae P8    32 head, virgin 1-day female    25 head, virgin 4-day female    19 head, virgin 20-day female    32 head, mated 1-day female    25 head, mated 4-day female    24 head, mated 20-day female    25 head, mated 1-day male    31 head, mated 4-day male    39 head, mated 20-day male    62 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    36 digestive system, larvae L3 wandering    138 digestive system, 1-day adult    60 digestive system, 4-day adult    46 digestive system, 20-day adult    50 fat body, larvae L3 wandering    21 fat body, white prepupae    56 fat body, pupae P8    25 carcass, larvae L3 wandering    157 carcass, 1-day adult    41 carcass, 4-day adult    50 carcass, 20-day adult    53 ovary, virgin 4-day female    112 ovary, mated 4-day female    93 testis, mated 4-day male    69 accessory gland, mated 4-day male    50 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    403 embryo 02-04hr    473 embryo 04-06hr    193 embryo 06-08hr    120 embryo 08-10hr    133 embryo 10-12hr    147 embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2    154 larva L3 12hr old    126 larva L3 puffstage 1-2    174 larva L3 puffstage 3-6    271 larva L3 puffstage 7-9    335 white prepupae new    364 white prepupae 12hr    144 white prepupae 24hr    147 pupae 2d postWPP    101 pupae 3d postWPP    92 pupae 4d postWPP    158 adult male 01day    126 adult male 05day    118 adult male 30day    119 adult female 01day    199 adult female 05day    236 adult female 30day    243 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (403) embryo 02-04hr  (473) embryo 04-06hr  (193) embryo 06-08hr  (120) embryo 08-10hr  (133) embryo 10-12hr  (147) embryo 12-14hr  (70) embryo 14-16hr  (66) embryo 16-18hr  (47) embryo 18-20hr  (43) embryo 20-22hr  (48) embryo 22-24hr  (44) larva L1  (70) larva L2  (154) larva L3 12hr old  (126) larva L3 puffstage 1-2  (174) larva L3 puffstage 3-6  (271) larva L3 puffstage 7-9  (335) white prepupae new  (364) white prepupae 12hr  (144) white prepupae 24hr  (147) pupae 2d postWPP  (101) pupae 3d postWPP  (92) pupae 4d postWPP  (158) adult male 01day  (126) adult male 05day  (118) adult male 30day  (119) adult female 01day  (199) adult female 05day  (236) adult female 30day  (243) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (403) embryo 02-04hr  (473) embryo 04-06hr  (193) embryo 06-08hr  (120) embryo 08-10hr  (133) embryo 10-12hr  (147) embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2  (154) larva L3 12hr old  (126) larva L3 puffstage 1-2  (174) larva L3 puffstage 3-6  (271) larva L3 puffstage 7-9  (335) white prepupae new  (364) white prepupae 12hr  (144) white prepupae 24hr  (147) pupae 2d postWPP  101 pupae 3d postWPP    92 pupae 4d postWPP  (158) adult male 01day  (126) adult male 05day  (118) adult male 30day  (119) adult female 01day  (199) adult female 05day  (236) adult female 30day  (243) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    403 embryo 02-04hr    473 embryo 04-06hr    193 embryo 06-08hr    120 embryo 08-10hr    133 embryo 10-12hr    147 embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2    154 larva L3 12hr old    126 larva L3 puffstage 1-2    174 larva L3 puffstage 3-6    271 larva L3 puffstage 7-9    335 white prepupae new    364 white prepupae 12hr    144 white prepupae 24hr    147 pupae 2d postWPP    101 pupae 3d postWPP    92 pupae 4d postWPP    158 adult male 01day    126 adult male 05day    118 adult male 30day    119 adult female 01day    199 adult female 05day    236 adult female 30day    243 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    403 embryo 02-04hr    473 embryo 04-06hr    193 embryo 06-08hr    120 embryo 08-10hr    133 embryo 10-12hr    147 embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2    154 larva L3 12hr old    126 larva L3 puffstage 1-2    174 larva L3 puffstage 3-6    271 larva L3 puffstage 7-9    335 white prepupae new    364 white prepupae 12hr    144 white prepupae 24hr    147 pupae 2d postWPP    101 pupae 3d postWPP    92 pupae 4d postWPP    158 adult male 01day    126 adult male 05day    118 adult male 30day    119 adult female 01day    199 adult female 05day    236 adult female 30day    243 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (403) embryo 02-04hr  (473) embryo 04-06hr  (193) embryo 06-08hr  (120) embryo 08-10hr  (133) embryo 10-12hr  (147) embryo 12-14hr  (70) embryo 14-16hr  (66) embryo 16-18hr  (47) embryo 18-20hr  (43) embryo 20-22hr  (48) embryo 22-24hr  (44) larva L1  (70) larva L2  (154) larva L3 12hr old  (126) larva L3 puffstage 1-2  (174) larva L3 puffstage 3-6  (271) larva L3 puffstage 7-9  (335) white prepupae new  (364) white prepupae 12hr  (144) white prepupae 24hr  (147) pupae 2d postWPP  (101) pupae 3d postWPP  (92) pupae 4d postWPP  (158) adult male 01day  (126) adult male 05day  (118) adult male 30day  (119) adult female 01day  (199) adult female 05day  (236) adult female 30day  (243) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (403) embryo 02-04hr  (473) embryo 04-06hr  (193) embryo 06-08hr  120 embryo 08-10hr  133 embryo 10-12hr  (147) embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2  (154) larva L3 12hr old  126 larva L3 puffstage 1-2  (174) larva L3 puffstage 3-6  (271) larva L3 puffstage 7-9  (335) white prepupae new  (364) white prepupae 12hr  (144) white prepupae 24hr  (147) pupae 2d postWPP  101 pupae 3d postWPP    92 pupae 4d postWPP  (158) adult male 01day  126 adult male 05day  118 adult male 30day  119 adult female 01day  (199) adult female 05day  (236) adult female 30day  (243) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    403 embryo 02-04hr    473 embryo 04-06hr    193 embryo 06-08hr    120 embryo 08-10hr    133 embryo 10-12hr    147 embryo 12-14hr    70 embryo 14-16hr    66 embryo 16-18hr    47 embryo 18-20hr    43 embryo 20-22hr    48 embryo 22-24hr    44 larva L1    70 larva L2    154 larva L3 12hr old    126 larva L3 puffstage 1-2    174 larva L3 puffstage 3-6    271 larva L3 puffstage 7-9    335 white prepupae new    364 white prepupae 12hr    144 white prepupae 24hr    147 pupae 2d postWPP    101 pupae 3d postWPP    92 pupae 4d postWPP    158 adult male 01day    126 adult male 05day    118 adult male 30day    119 adult female 01day    199 adult female 05day    236 adult female 30day    243 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    390 Schneider line 2 Sg4    288 embryonic 1182-4H    105 embryonic GM2    157 embryonic Kc167    262 embryonic S1    173 embryonic S3    277 leg disc CME L1    127 wing disc CME-W2    241 wing disc ML-DmD8    199 wing disc ML-DmD9    131 wing disc ML-DmD16-c3    118 wing disc ML-DmD21    228 wing disc ML-DmD32    115 haltere disc ML-DmD17-c3    168 eye-antennal disc ML-DmD11    119 antennal disc ML-DmD20-c5    219 mixed discs ML-DmD4-c1    211 CNS ML-DmBG1-c1    137 CNS ML-DmBG2-c2    381 tumorous blood cells mbn2    158 ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (390) Schneider line 2 Sg4  (288) embryonic 1182-4H  (105) embryonic GM2  (157) embryonic Kc167  (262) embryonic S1  (173) embryonic S3  (277) leg disc CME L1  (127) wing disc CME-W2  (241) wing disc ML-DmD8  (199) wing disc ML-DmD9  (131) wing disc ML-DmD16-c3  (118) wing disc ML-DmD21  (228) wing disc ML-DmD32  (115) haltere disc ML-DmD17-c3  (168) eye-antennal disc ML-DmD11  (119) antennal disc ML-DmD20-c5  (219) mixed discs ML-DmD4-c1  (211) CNS ML-DmBG1-c1  (137) CNS ML-DmBG2-c2  (381) tumorous blood cells mbn2  (158) ovary fGS/OSS  (47) ovary OSC  (48) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (390) Schneider line 2 Sg4  (288) embryonic 1182-4H  105 embryonic GM2  (157) embryonic Kc167  (262) embryonic S1  (173) embryonic S3  (277) leg disc CME L1  (127) wing disc CME-W2  (241) wing disc ML-DmD8  (199) wing disc ML-DmD9  (131) wing disc ML-DmD16-c3  (118) wing disc ML-DmD21  (228) wing disc ML-DmD32  (115) haltere disc ML-DmD17-c3  (168) eye-antennal disc ML-DmD11  (119) antennal disc ML-DmD20-c5  (219) mixed discs ML-DmD4-c1  (211) CNS ML-DmBG1-c1  (137) CNS ML-DmBG2-c2  (381) tumorous blood cells mbn2  (158) ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    390 Schneider line 2 Sg4    288 embryonic 1182-4H    105 embryonic GM2    157 embryonic Kc167    262 embryonic S1    173 embryonic S3    277 leg disc CME L1    127 wing disc CME-W2    241 wing disc ML-DmD8    199 wing disc ML-DmD9    131 wing disc ML-DmD16-c3    118 wing disc ML-DmD21    228 wing disc ML-DmD32    115 haltere disc ML-DmD17-c3    168 eye-antennal disc ML-DmD11    119 antennal disc ML-DmD20-c5    219 mixed discs ML-DmD4-c1    211 CNS ML-DmBG1-c1    137 CNS ML-DmBG2-c2    381 tumorous blood cells mbn2    158 ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    390 Schneider line 2 Sg4    288 embryonic 1182-4H    105 embryonic GM2    157 embryonic Kc167    262 embryonic S1    173 embryonic S3    277 leg disc CME L1    127 wing disc CME-W2    241 wing disc ML-DmD8    199 wing disc ML-DmD9    131 wing disc ML-DmD16-c3    118 wing disc ML-DmD21    228 wing disc ML-DmD32    115 haltere disc ML-DmD17-c3    168 eye-antennal disc ML-DmD11    119 antennal disc ML-DmD20-c5    219 mixed discs ML-DmD4-c1    211 CNS ML-DmBG1-c1    137 CNS ML-DmBG2-c2    381 tumorous blood cells mbn2    158 ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (390) Schneider line 2 Sg4  (288) embryonic 1182-4H  (105) embryonic GM2  (157) embryonic Kc167  (262) embryonic S1  (173) embryonic S3  (277) leg disc CME L1  (127) wing disc CME-W2  (241) wing disc ML-DmD8  (199) wing disc ML-DmD9  (131) wing disc ML-DmD16-c3  (118) wing disc ML-DmD21  (228) wing disc ML-DmD32  (115) haltere disc ML-DmD17-c3  (168) eye-antennal disc ML-DmD11  (119) antennal disc ML-DmD20-c5  (219) mixed discs ML-DmD4-c1  (211) CNS ML-DmBG1-c1  (137) CNS ML-DmBG2-c2  (381) tumorous blood cells mbn2  (158) ovary fGS/OSS  (47) ovary OSC  (48) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (390) Schneider line 2 Sg4  (288) embryonic 1182-4H  105 embryonic GM2  (157) embryonic Kc167  (262) embryonic S1  (173) embryonic S3  (277) leg disc CME L1  127 wing disc CME-W2  (241) wing disc ML-DmD8  (199) wing disc ML-DmD9  131 wing disc ML-DmD16-c3  118 wing disc ML-DmD21  (228) wing disc ML-DmD32  115 haltere disc ML-DmD17-c3  (168) eye-antennal disc ML-DmD11  119 antennal disc ML-DmD20-c5  (219) mixed discs ML-DmD4-c1  (211) CNS ML-DmBG1-c1  137 CNS ML-DmBG2-c2  (381) tumorous blood cells mbn2  (158) ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    390 Schneider line 2 Sg4    288 embryonic 1182-4H    105 embryonic GM2    157 embryonic Kc167    262 embryonic S1    173 embryonic S3    277 leg disc CME L1    127 wing disc CME-W2    241 wing disc ML-DmD8    199 wing disc ML-DmD9    131 wing disc ML-DmD16-c3    118 wing disc ML-DmD21    228 wing disc ML-DmD32    115 haltere disc ML-DmD17-c3    168 eye-antennal disc ML-DmD11    119 antennal disc ML-DmD20-c5    219 mixed discs ML-DmD4-c1    211 CNS ML-DmBG1-c1    137 CNS ML-DmBG2-c2    381 tumorous blood cells mbn2    158 ovary fGS/OSS    47 ovary OSC    48 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3    148 Cadmium 50 mM 12 hrs, larvae L3    153 Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    127 Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    149 Rotenone 2 μg 12 hrs, larvae L3    104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (50) cold shock, 4-day adult  (50) heat shock, 4-day adult  (89) Cadmium 50 mM 6 hrs, larvae L3  (148) Cadmium 50 mM 12 hrs, larvae L3  (153) Cadmium 50 mM 48 hrs, 4-day adult  (73) Cadmium 100 mM 48 hrs, 4-day adult  (89) Copper 0.5 mM 12 hrs, larvae L3  (47) Copper 15 mM 48 hrs, 4-day adult  (58) Zinc 5 mM 12 hrs, larvae L3  (53) Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (127) Ethanol 5% 3 hrs, larvae L3  (95) Ethanol 10% 3 hrs, larvae L3  (85) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (87) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (83) Caffeine 25 mg/ml 48 hrs, 4-day adult  (42) Paraquat 5 mM 48 hrs, 4-day adult  (86) Paraquat 10 mM 48 hrs, 4-day adult  (149) Rotenone 2 μg 12 hrs, larvae L3  (104) Rotenone 8 μg 12 hrs, larvae L3  (94) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3  (148) Cadmium 50 mM 12 hrs, larvae L3  (153) Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3  (127) Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult  (149) Rotenone 2 μg 12 hrs, larvae L3  104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3    148 Cadmium 50 mM 12 hrs, larvae L3    153 Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    127 Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    149 Rotenone 2 μg 12 hrs, larvae L3    104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3    148 Cadmium 50 mM 12 hrs, larvae L3    153 Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    127 Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    149 Rotenone 2 μg 12 hrs, larvae L3    104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (50) cold shock, 4-day adult  (50) heat shock, 4-day adult  (89) Cadmium 50 mM 6 hrs, larvae L3  (148) Cadmium 50 mM 12 hrs, larvae L3  (153) Cadmium 50 mM 48 hrs, 4-day adult  (73) Cadmium 100 mM 48 hrs, 4-day adult  (89) Copper 0.5 mM 12 hrs, larvae L3  (47) Copper 15 mM 48 hrs, 4-day adult  (58) Zinc 5 mM 12 hrs, larvae L3  (53) Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (127) Ethanol 5% 3 hrs, larvae L3  (95) Ethanol 10% 3 hrs, larvae L3  (85) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (87) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (83) Caffeine 25 mg/ml 48 hrs, 4-day adult  (42) Paraquat 5 mM 48 hrs, 4-day adult  (86) Paraquat 10 mM 48 hrs, 4-day adult  (149) Rotenone 2 μg 12 hrs, larvae L3  (104) Rotenone 8 μg 12 hrs, larvae L3  (94) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3  (148) Cadmium 50 mM 12 hrs, larvae L3  (153) Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3  127 Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult  (149) Rotenone 2 μg 12 hrs, larvae L3  104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    50 cold shock, 4-day adult    50 heat shock, 4-day adult    89 Cadmium 50 mM 6 hrs, larvae L3    148 Cadmium 50 mM 12 hrs, larvae L3    153 Cadmium 50 mM 48 hrs, 4-day adult    73 Cadmium 100 mM 48 hrs, 4-day adult    89 Copper 0.5 mM 12 hrs, larvae L3    47 Copper 15 mM 48 hrs, 4-day adult    58 Zinc 5 mM 12 hrs, larvae L3    53 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    127 Ethanol 5% 3 hrs, larvae L3    95 Ethanol 10% 3 hrs, larvae L3    85 Caffeine 1.5 mg/ml 4 hrs, larvae L3    87 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    83 Caffeine 25 mg/ml 48 hrs, 4-day adult    42 Paraquat 5 mM 48 hrs, 4-day adult    86 Paraquat 10 mM 48 hrs, 4-day adult    149 Rotenone 2 μg 12 hrs, larvae L3    104 Rotenone 8 μg 12 hrs, larvae L3    94 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_02 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • LD08717 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029134 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12323-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Prosβ5GD7568 lethal, with Scer\GAL4pnr-MD237 Prosβ5GD7568 Other Phenotypes Allele neuroanatomy defective | larval stage Prosβ5GD7568 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage Prosβ5GD7568 ganglion mother cell | larval stage Prosβ5GD7568
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Prosβ5 Class Mutagen Stocks Known lesion Prosβ5CB-0768-3 1 -- Prosβ5DP00085 1 -- Prosβ5EP2619 P-element activity 1 Yes Prosβ5f03598 piggyBac transposase 1 -- Prosβ5Δ1 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Prosβ5 Class Mutagen Stocks Known lesion Prosβ5GD7568 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Prosβ5HMS00119 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Prosβ5KK101404 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Prosβ5dsRNA.cWa in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7112 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}dgoEP2619 NA P{EPgy2}EY00934 NA P{GSV1}C839C839 NA P{Mae-UAS.6.11}Prosβ5DP00085 NA miscellaneous insertions P{RS3}Prosβ5CB-0768-3 NA P{UAST-YFP.RabX5.T78N}01 NA PBac{WH}Prosβ5f03598 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell proliferation inferred from mutant phenotype (Goshima et al., 2007) centrosome organization inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
proteasome core complex inferred from direct assay (Sumegi et al., 2003)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
endopeptidase activity inferred from sequence or structural similarity with UniProtKB:P28074 (FlyBase, 1992-) non-traceable author statement (Renault, 1999.12.20) contributes_to endopeptidase activity inferred by curator from GO:0005839 (Sumegi et al., 2003) threonine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR001353, InterPro:IPR023333 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
proteasomal ubiquitin-dependent protein catabolic process inferred by curator from GO:0005839 (Sumegi et al., 2003) proteolysis involved in cellular protein catabolic process inferred from electronic annotation with InterPro:IPR000243, InterPro:IPR001353, InterPro:IPR016050 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
proteasome core complex inferred from sequence or structural similarity with UniProtKB:P28074 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Prosβ5 - Aats-his anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Aats-lys anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - AnxB10 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Aos1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Arp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Ate1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG11191 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG11885 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - CG12299 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG12321 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - CG12343 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG13319 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - CG13539 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG15011 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG15717 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - CG1598 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG17207 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG17834 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG18335 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG2046 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - CG30008 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG31917 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG3526 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG3795 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG3812 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG4278 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG43444 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG5196 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG5382 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG5577 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG5642 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG6321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG8314 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - CG8525 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - CG9686 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Cg25C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Corp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Csp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Cyp4g15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Der-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Eig71Ed anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Eno experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Ime4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Int6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Klc experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - M6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Mdh2 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Moe experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Mtpα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - NAA20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - NimB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Patsas anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Pgam5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Pomp experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Prosα1 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosα3 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosα4 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosα5 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosα6 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosα6T anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Prosα7 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosβ1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosβ5 - Prosβ2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosβ2R2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Prosβ3 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosβ4 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosβ5 - Prosβ4R1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Prosβ4R2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Prosβ6 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prosβ7 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Prx2540-2 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - RASSF8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - REG experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - RpIII128 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - RpS5a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Rpn1 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn11 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosβ5 - Rpn13 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn2 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn6 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn7 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn8 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpn9 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Rpt2 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpt3 experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Rpt4 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosβ5 - Rpt5 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosβ5 - Rpt6 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosβ5 - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Sh3β experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - Smn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Uch-L5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Uvrag anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - Vha100-4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - aur anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - ave anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - dlg1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - fbp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - fzr2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - mRpS33 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - scu experimental knowledge based (Guruharsha et al., 2011) Prosβ5 - stet anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosβ5 - vkg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Prosβ5 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69549 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029134 DroID - A comprehensive database of gene and protein interactions. • FBgn0029134 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45269
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QZ865 Dipteran inclusive ortholog search EOG6DJK5F Insect inclusive ortholog search EOG6DJHS7 Arthropod inclusive ortholog search EOG65MKN3 Metazoa inclusive ortholog search EOG63Z6XC
Orthologs in Drosophila Species (EOG6QZ865)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Prosβ5     Drosophila simulans   Dsim\GD10758 Y   Drosophila sechellia   Dsec\GM21240 Y   Drosophila erecta   Dere\GG20151 Y   Drosophila yakuba   Dyak\Prosβ5 Y   Drosophila ananassae   Dana\GF13778 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11556 Y   Drosophila willistoni   Dwil\GK15836 Y   Drosophila virilis   Dvir\GJ21921 Y   Drosophila mojavensis   Dmoj\GI18886 Y   Drosophila grimshawi   Dgri\GH20460 Y
Orthologs in non-Drosophila Dipterans (EOG6DJK5F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013236   Anopheles gambiae Malaria mosquito  Agam\AGAP010718   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017386
Orthologs in non-Dipteran Insects (EOG6DJHS7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC411206   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010473   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14396   Atta cephalotes Leafcutter ant  Acep\ACEP26253   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17907   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21969   Linepithema humile Argentine ant  Lhum\LH13661   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16915   Solenopsis invicta Red fire ant  Sinv\SINV10011   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000065   Bombyx mori Silkmoth  Bmor\BGIBMGA004376   Pediculus humanus Human body louse  Phum\PHUM245170   Tribolium castaneum Red flour beetle  Tcas\TC030625
Orthologs in non-Insect Arthropods (EOG65MKN3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_303318 Y Daphnia pulex Water flea  Dpux\JGI_V11_303852 Y Ixodes scapularis Deer tick  Isca\ISCW005987 Y
Orthologs in non-Arthropod Metazoa (EOG63Z6XC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\pbs-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014490gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028652gn Y Danio rerio Zebrafish  Drer\psmb5 Y Danio rerio Zebrafish  Drer\psmb8 Y Xenopus tropicalis Western clawed frog  Xtro\psmb8 Y Xenopus tropicalis Western clawed frog  Xtro\psmb5 Y Mus musculus House mouse  Mmus\Psmb5 Y Mus musculus House mouse  Mmus\Psmb8 Y Rattus norvegicus Norway rat  Rnor\Psmb5 Y Rattus norvegicus Norway rat  Rnor\Psmb8 Y Homo sapiens Human  Hsap\PSMB8 Y Homo sapiens Human  Hsap\PSMB5 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\PSMB8     Hsap\PSMB5
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10758 Drosophila sechellia Dsec\GM21240 Drosophila erecta Dere\GG20151 Drosophila yakuba Dyak\Prosβ5 Drosophila ananassae Dana\GF13778 Drosophila pseudoobscura pseudoobscura Dpse\GA11556 Drosophila willistoni Dwil\GK15836 Drosophila virilis Dvir\GJ21921 Drosophila mojavensis Dmoj\GI18886 Drosophila grimshawi Dgri\GH20460
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	34810 y1 sc* v1; P{TRiP.HMS00119}attP2 17077 w1118; P{EP}dgoEP2619 Prosβ5EP2619 21794 y1 w67c23; P{Mae-UAS.6.11}Prosβ5DP00085
Harvard	- PBac{WH}Prosβ5f03598
Kyoto	123322 w1118; P{RS3}Prosβ5CB-0768-3
VDRC	v107628 P{KK101404}VIE-260B v38659 w1118; P{GD7568}v38659 v38658 w1118; P{GD7568}v38658/TM3
Genomic Clones ( 1 )
	BACR13J10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 100 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD08717
Other clones	DPiM_LD08717 yak-ad_Prosbeta5 yak-em_Prosbeta5
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH03320 GH04561 GH05416 GH07604 GM07976 GM30339 LD06379 LD06910 LD28833 LP02869 LP08570 RE10534 RE10969 RE14474 RE39783 RE41684 RE43786 RE52416 RE59480 RE59776 RE59976 RE59977 RE60552 RE62050 RE64622 RE66256 RE71166 RH01932 RH02220 RH03666 RH06517 RH06584 RH14438 RH14660 RH17432 RH29594 RH31468 RH33212 RH33274 RH44737 RH45644 RH56641 RH61638 RH63518 SD14905 SD16025
Other clones	34047 74745 Dmel_dig_003273_0672_0442 EC13760 EK148416 EK154106 EK204949 FGA009E02 FGC029H06 FGM101C06 FGM315B11 RP001001431 RP001002927 RP001004403 RP001004878 RP001008043 RP001010683 RP001011460 RP001014635 RP001015762 RP001016085 RP001019279 RP001021058 RP001021389 RP001022199 RP001022777 RP001023984 RP001024293 RP001025159 RP001025488 RP001026216 RP001030270 RP001033273 RP001034974 RP001039578 RP001039901 RP001040765 RP001041916 RP001043075 RP001046562 RP001047290 RP001047818 RP001048008 RP001049863 RP001050492 RP001051150 RP001051362 RP001054712 RP001054982 RP001055648
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0029134 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45269
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Prosβ5 anon-WO0118547.116 (FlyBase, 1996-) Source for merge of: Prosβ5 CG12323 (Bayraktaroglu, 2000.8.7)
Additional comments	The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms. (FlyBase, 2009-) FlyBase curator comment: "EP2619" overexpression phenotype stated to be due to its effect on EST LD08717 (which corresponds to Prosβ5) but orientation of the P{EP} element suggests otherwise. (Pena-Rangel et al., 2002) Source for merge of Prosβ5 anon-WO0118547.116 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells does not affect the number of prophase centrosomes, but monastral bipolar spindles are formed during mitosis, suggesting that the centrosomes are fused. (Goshima et al., 2007) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) Area matching Drosophila ESTs AI064230 and AA263288. (Davies, 2001.3.15)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143757 NM_165801 RefSeq (Proteins) • NP_652014 NP_724974 DDBJ / EMBL / GenBank • AA263288 AA390473 AAF26416 AAF58748 AAL39526 AAM68751 AF217726 AI064230 AW941690 AX093992 AY069381 CZ486175 CZ486176 Entrez Gene - A searchable database of RefSeq genes. • 45269 UniProtKB/TrEMBL • Q7K148 Q9NHX7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase T1A, proteasome beta-subunit (IPR000243) Proteasome, subunit alpha/beta (IPR001353) Proteasome, beta-type subunit, conserved site (IPR016050) Proteasome B-type subunit (IPR023333) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD08717
Linkouts
	BioGRID - A database of protein and genetic interactions • 69549 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029134 DroID - A comprehensive database of gene and protein interactions. • FBgn0029134 DRSC - Results from RNAi screens. • FBgn0029134 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029134 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12323-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0029134 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 205676 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45269 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45269 modMine - Data generated by the modENCODE project. • FBgn0029134
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	anon-WO0118547.116   beta-5 (Goshima et al., 2007) Beta-5 (Lundgren et al., 2005) beta5 (Yano et al., 2012) CG12323 (Rawlings, 2001.2.12, Goshima et al., 2007, Molnar et al., 2006, Barbash and Lorigan, 2007, Keleman et al., 2009.8.5, Ma et al., 2002, Gandhi et al., 2007, Juhász et al., 2007, Ni et al., 2011.7.19, Cammarato et al., 2011, Lundgren et al., 2005, Belote and Zhong, 2009, Yano et al., 2012) dβ5 (Wojcik and DeMartino, 2002, Vernace et al., 2007) LD08717 (Pena-Rangel et al., 2002) Pros b5 (Molnar et al., 2006) Prosbeta5 (Giot et al., 2003, Lindquist et al., 2011, Carbone et al., 2009, Barbash and Lorigan, 2007, Zhou et al., 2008, Neumüller et al., 2011, Lundgren et al., 2005, Gurudatta et al., 2012) Prosβ5 (Goshima et al., 2007, Zhang et al., 2007, Ma et al., 2002, Bandyopadhyay et al., 2006) PSMB5 (Lindquist et al., 2011) β5 (Belote and Zhong, 2009)
Name Synonym	20S proteasome (Bohm et al., 2000) Prosbeta5 (Cammarato et al., 2011) Prosβ5   Proteasome β5 subunit   β5 (Nickell et al., 2009)
Secondary FlyBase IDs
	FBgn0033553 FBgn0062234
References ( 58 )
Generate a list of	All references relating to this gene ( 58 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 12 ) Review  ( 1 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 1 ) FlyBase analysis  ( 4 ) Patent  ( 1 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 6 )
	Velentzas et al., 2013, Cell Biol. Toxicol. 29(1): 13--37 Detrimental effects of proteasome inhibition activity in Drosophila melanogaster: implication of ER stress, autophagy, and apoptosis. [FBrf0220377] Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132 The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796] Yano et al., 2012, J. Neurogenet. 26(1): 53--63 Identification of proteasome components required for apical localization of chaoptin using functional genomics. [FBrf0217843] Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...