FlyBase Gene Report: Dmel\ssh

General Information

Symbol

Dmel\ssh

Species

D. melanogaster

Name

slingshot

Annotation Symbol

CG6238

Feature Type

protein_coding_gene

FlyBase ID

FBgn0029157

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

Protein-serine/threonine phosphatase (3.1.3.16)

Protein-tyrosine-phosphatase (3.1.3.48)

Gene Snapshot

slingshot (ssh) encodes a protein that plays a pivotal role in actin dynamics by reactivating/dephosphorylating the product of tsr in vivo. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(3)01207, MKP, Mkph

Key Links

Genomic Location

Cytogenetic map

96B10-96B11

Sequence location

3R:24,938,710..24,944,956 [-]

Recombination map

3-86

RefSeq locus

NT_033777 REGION:24938710..24944956

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

DUAL SPECIFICITY PHOSPHATASES

Protein Family (UniProt)

Belongs to the protein-tyrosine phosphatase family. (Q6NN85)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

actin binding; NOT MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Predictions / Assertions

actin binding; phosphoprotein phosphatase activity; protein tyrosine phosphatase activity

Catalytic Activity (EC)

Experimental Evidence

[a protein]-serine/threonine phosphate + H(2)O = [a protein]- serine/threonine + phosphate (3.1.3.16)

Predictions / Assertions

[a protein]-serine/threonine phosphate + H(2)O = [a protein]- serine/threonine + phosphate (3.1.3.16)

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate (3.1.3.48)

Summaries

Gene Group (FlyBase)

DUAL SPECIFICITY PHOSPHATASES -: Dual Specificity Phosphatases (DSP) can dephosphorylate both tyrosine and serine/threonine residues. Additionally, DSPs may target a much larger and diverse set of substrates, including phosphoinositide lipids, RNA 5'-triphosphate and carbohydrates. (Adapted from FBrf0227974).

Protein Function (UniProtKB)

Protein phosphatase which regulates actin filament dynamics (PubMed:11832213, PubMed:16169194). Dephosphorylates and activates the actin binding/depolymerizing factor tsr/cofilin, which subsequently binds to actin filaments and stimulates their disassembly (PubMed:11832213). Required for axon growth (PubMed:15572110).

(UniProt, Q6NN85)

Summary (Interactive Fly)

phosphatase - plays a pivotal role in actin dynamic by reactivating the actin depolymerizing factor cofilin

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\ssh or the JBrowse view of Dmel\ssh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ssh-RA

FBtr0084799

NM_079768

4780

1192

ssh-RB

FBtr0084800

NM_170184

4254

1193

ssh-RC

FBtr0301612

NM_001170245

4186

1046

ssh-RD

FBtr0301613

NM_001170246

4419

1045

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

ssh-PA

FBpp0084174

131.1

1192

9.17

NP_524492

AAF56372

ssh-PB

FBpp0084175

131.5

1193

9.05

NP_733063

AAN14027

ssh-PC

FBpp0290827

115.4

1046

8.72

NP_001163716

ACZ95010

ssh-PD

FBpp0290828

115.0

1045

8.87

NP_001163717

ACZ95011

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with actin.

(UniProt, Q6NN85)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ssh using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ssh_1
ssh_2

Linkouts

Gene Ontology (19 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

actin binding

inferred from direct assay

(assigned by UniProt )

(Niwa et al., 2002)

NOT MAP kinase tyrosine/serine/threonine phosphatase activity

inferred from direct assay

(Rogers et al., 2005)

phosphoprotein phosphatase activity

inferred from direct assay

(assigned by UniProt )

(Niwa et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

actin binding

inferred from biological aspect of ancestor with PANTHER:PTN000807232

(assigned by GO_Central )

(Gaudet et al., 2011)

phosphoprotein phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN000807232

(assigned by GO_Central )

(Gaudet et al., 2011)

protein tyrosine phosphatase activity

inferred from electronic annotation with InterPro:IPR016130

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (13 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

axonogenesis

inferred from mutant phenotype

(assigned by UniProt )

(Ng and Luo, 2004)

cell morphogenesis

inferred from mutant phenotype

(assigned by UniProt )

(Niwa et al., 2002)

compound eye development

inferred from genetic interaction with FLYBASE:mbt; FB:FBgn0025743

(Menzel et al., 2007)

mitotic cell cycle

inferred from mutant phenotype

(Chen et al., 2007)

mushroom body development

inferred from mutant phenotype

(Ng and Luo, 2004)

negative regulation of actin filament polymerization

inferred from direct assay

(assigned by UniProt )

(Niwa et al., 2002)

nuclear migration

inferred from mutant phenotype

(assigned by UniProt )

(Rogers et al., 2005)

protein dephosphorylation

inferred from mutant phenotype

(assigned by UniProt )

(Ng and Luo, 2004, Niwa et al., 2002)

regulation of actin filament polymerization

inferred from mutant phenotype

(assigned by UniProt )

(Rogers et al., 2005)

regulation of actin polymerization or depolymerization

inferred from mutant phenotype

(Niwa et al., 2002)

regulation of axonogenesis

inferred from mutant phenotype

(Ng and Luo, 2004)

inferred from genetic interaction with FLYBASE:sick; FB:FBgn0263873

(Abe et al., 2014)

regulation of lamellipodium assembly

inferred from mutant phenotype

(Iwasa and Mullins, 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

actin cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN000807232

(assigned by GO_Central )

(Gaudet et al., 2011)

negative regulation of actin filament polymerization

inferred from biological aspect of ancestor with PANTHER:PTN000807232

(assigned by GO_Central )

(Gaudet et al., 2011)

protein dephosphorylation

non-traceable author statement

(Castellani and Rougon, 2002)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

apical part of cell

inferred from direct assay

(assigned by UniProt )

(Rogers et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000807232

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

apical part of cell

inferred from direct assay

(assigned by UniProt )

(Rogers et al., 2005)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}ssh^SE148

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

eye disc

(Bloomington Drosophila Stock Center, 2019)

photoreceptor cell

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ssh in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ssh

View exonic expression by tissue for Dmel\ssh

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029157

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029157

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6238-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6238

Flygut - An atlas of the Drosophila adult midgut

FBgn0029157

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ssh

Transgenic constructs containing regulatory region of ssh

Deletions and Duplications ( 5 )

Disrupted in

Df(3R)BSC318

(Christensen et al., 2007.10.29)

Df(3R)BSC655

(Christensen et al., 2008.12.28)

Df(3R)XTA1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Not disrupted in

Df(3R)96B

(Nairz, 2002.6.28)

Df(3R)crb87-5

(Spradling et al., 1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | embryonic stage | maternal effect

ssh⁰¹²⁰⁷

lethal | embryonic stage | recessive

ssh⁰¹²⁰⁷

lethal | recessive

ssh⁰¹²⁰⁷

lethal - all die before end of embryonic stage | recessive

ssh⁰¹²⁰⁷

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

ssh^GD11869

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ssh^GD11869

viable, with Scer\GAL4^Mef2.PR

ssh^GD11869

viable, with Scer\GAL4^tin.CΔ4

ssh^GD11869

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

ssh^HMS01862

Other Phenotypes

Allele

chemical resistant | adult stage, with Scer\GAL4^Tub.PU

ssh^UAS.Tag:HA

neuroanatomy defective

ssh^1-63

neuroanatomy defective | somatic clone

some die during embryonic stage | recessive

ssh⁰¹²⁰⁷

some die during pupal stage, with Scer\GAL4^pnr-MD237

ssh^GD11869

visible | somatic clone

Phenotype manifest in

Allele

actin filament | cell autonomous | ectopic | somatic clone

ssh^1-11

adult mushroom body & axon | somatic clone

antennal lobe & axon | somatic clone

apical junction complex & ommatidial cluster | somatic clone

ssh^1-11

denticle belt

ssh⁰¹²⁰⁷

denticle belt | maternal effect

ssh⁰¹²⁰⁷

embryo | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

ssh^HMS01862

embryonic/first instar larval cuticle

ssh⁰¹²⁰⁷

embryonic/first instar larval cuticle | maternal effect

ssh⁰¹²⁰⁷

eye | somatic clone

filamentous actin & ommatidial cluster | ectopic | somatic clone

ssh^1-11

filamentous actin | somatic clone

ssh^1-11

macrochaeta & mesothoracic tergum | somatic clone

ssh^1-11

medial lobe of adult mushroom body | somatic clone

ssh^1-11

mushroom body alpha-lobe

ssh^1-63

mushroom body beta-lobe

ssh^1-63

mushroom body medial lobe | somatic clone

nucleus & ommatidial cluster | somatic clone

ssh^1-11

ommatidium | somatic clone

ssh^26-1

optic lobe neuron & axon | somatic clone

rhabdomere | somatic clone

ssh^26-1

vertical lobe of adult mushroom body | somatic clone

wing hair

ssh⁵¹

wing hair, with Scer\GAL4^ap-md544

ssh^{dsRNA.UAS.GFP}

wing hair | somatic clone

ssh^1-11

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (26)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SSH2

9 of 15

Yes

Hsap\SSH3

9 of 15

Yes

Hsap\SSH1

8 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\STYX

1 of 15

Hsap\STYXL1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (28)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ssh3

10 of 15

Yes

Mmus\Ssh2

8 of 15

Yes

Mmus\Ssh1

7 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Gm14698

1 of 15

Mmus\Styxl1

1 of 15

Yes

Mmus\Styx

1 of 15

Rattus norvegicus (Norway rat) (23)

Rnor\Ssh3

8 of 13

Yes

Rnor\Ssh1

7 of 13

Yes

Rnor\Ssh2

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\LOC100909469

1 of 13

Yes

Rnor\LOC108348179

1 of 13

Rnor\Styxl1

1 of 13

Yes

Rnor\Styx

1 of 13

Xenopus tropicalis (Western clawed frog) (19)

Xtro\ssh1

4 of 12

Yes

Xtro\ssh2

4 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\ssh3

1 of 12

Yes

Xtro\styxl1

1 of 12

Yes

Xtro\styx

1 of 12

Danio rerio (Zebrafish) (33)

Drer\si:ch211-203d1.3

8 of 15

Yes

Drer\ssh2a

7 of 15

Yes

Drer\ssh1a

6 of 15

Yes

Drer\ssh1b

6 of 15

Yes

Drer\ssh2b

6 of 15

Yes

Drer\dusp4

2 of 15

Drer\dusp16

2 of 15

Drer\si:dkey-175m17.7

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\LOC100150565

1 of 15

Drer\si:ch211-121a2.2

1 of 15

Drer\si:ch211-195b15.8

1 of 15

Drer\si:ch211-223p8.8

1 of 15

Drer\styx

1 of 15

Drer\styxl1

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\F13D11.3

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\MSG5

3 of 15

Yes

Scer\PPS1

2 of 15

Scer\SDP1

2 of 15

Scer\YVH1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pmp1

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919028M )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19465

Drosophila persimilis

Dper\GL23129

Drosophila persimilis

Dper\GL23130

Drosophila willistoni

Dwil\GK14268

Drosophila virilis

Dvir\GJ22910

Drosophila mojavensis

Dmoj\GI10555

Drosophila grimshawi

Dgri\GH21504

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501IJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010104

Glossina morsitans

Tsetse fly

Gmor\GMOY005007

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11804

Mayetiola destructor

Hessian fly

Mdes\Mdes008457

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012935

Anopheles darlingi

American malaria mosquito

Adar\ADAC000313

Anopheles gambiae

Malaria mosquito

Agam\AGAP000898

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018450

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019559

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W021W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006843

Danaus plexippus

Monarch butterfly

Dple\DPOGS206061-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL014066

Apis florea

Little honeybee

Aflo\2772513828

Apis mellifera

Western honey bee

Amel\GB51748

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24785

Bombus terrestris

Buff-tailed bumblebee

Bter\15210702

Linepithema humile

Argentine ant

Lhum\LH23280

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00100e

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001010

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002818

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07986

Tribolium castaneum

Red flour beetle

Tcas\TC034442

Pediculus humanus

Human body louse

Phum\PHUM176310

Rhodnius prolixus

Kissing bug

Rpro\RPRC004855

Cimex lectularius

Bed bug

Clec\CLEC001738

Cimex lectularius

Bed bug

Clec\CLEC001740

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008825

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13037

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01ZG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005885

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58488.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58489.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_47512

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0249 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ssh_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dusp14/18/21

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ssh

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Styx

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Jak_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dusp6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016407gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dsp3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dsp6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dusp16

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dusp1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009331

Ciona intestinalis

Vase tunicate

Cint\dusp1.2.4.5

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007989

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007532

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000003538

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000005007

Gallus gallus

Domestic chicken

Ggal\DUSP6

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000011419

Gallus gallus

Domestic chicken

Ggal\DUSP19

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000003706

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000008581

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028155

Gallus gallus

Domestic chicken

Ggal\SSH2

Gallus gallus

Domestic chicken

Ggal\SSH1

Gallus gallus

Domestic chicken

Ggal\DUSP13

Gallus gallus

Domestic chicken

Ggal\DUSP26

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000029003

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026085

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000006647

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027641

Gallus gallus

Domestic chicken

Ggal\DUPD1

Gallus gallus

Domestic chicken

Ggal\DUSP18

Gallus gallus

Domestic chicken

Ggal\DUSP27

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

puc

3 of 10

CG10089

2 of 10

CG15528

2 of 10

Mkp3

2 of 10

Mkp

2 of 10

CG7378

1 of 10

MKP-4

1 of 10

PTPMT1

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ssh^NIG.6238R

ameliorates amyotrophic lateral sclerosis

modeled by Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SSH2; slingshot protein phosphatase 2

9 of 15

606779

SSH3; slingshot protein phosphatase 3

9 of 15

606780

SSH1; slingshot protein phosphatase 1

8 of 15

606778

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ssh - Deaf1

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ng and Luo, 2004)

enhanceable

(Niwa et al., 2002)

suppressible

(Ng and Luo, 2004)

suppressible

(Ng and Luo, 2004)

suppressible

(Ng and Luo, 2004)

suppressible

(Ng and Luo, 2004)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Abe et al., 2014)

suppressible

(Abe et al., 2014, Ng and Luo, 2004)

mbt

suppressible

ssh

(Menzel et al., 2007)

Pax

suppressible

ssh

(Chen et al., 2005)

sev

suppressible

ssh

(Sese et al., 2006)

suppressible

(Abe et al., 2014)

enhanceable

(Pham et al., 2008)

Vap33

suppressible

ssh

(Deivasigamani et al., 2014)

External Data

Subunit Structure (UniProtKB)

Interacts with actin.

(UniProt, Q6NN85 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67907

DroID - A comprehensive database of gene and protein interactions.

FBgn0029157

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

42986

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-86

Cytogenetic map

96B10-96B11

Sequence location

3R:24,938,710..24,944,956 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96B10-96B11

Limits computationally determined from genome sequence between P{EP}CycB3^EP3127 and P{PZ}ssh⁰¹²⁰⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

96B-96B

(Uemura, 2000.2.7)

96B10-96B11

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

96B10-96B11

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-86

(FlyBase, 2016)

3-85.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Bloomington

9115

w^*; P{UAS-ssh.CS}43

9112

y¹ w^*; P{UAS-ssh.N}30

9113

y¹ w^*; P{UAS-ssh.N}45

9114

y¹ w^*; P{UAS-ssh.CS}11

81559

w^*; P{ST.lexA::HG}ssh^SE148/TM6B, Tb¹

38948

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01862}attP2

11524

ry⁵⁰⁶ P{PZ}ssh⁰¹²⁰⁷/TM3, ry^RK Sb¹ Ser¹

9110

y¹ w^*; P{neoFRT}82B ssh^1-63/TM3, y⁺ Ser¹

VDRC

v107998

P{KK100586}VIE-260B

v318687

PBac{fTRG00844.sfGFP-TVPTBF}VK00002

v30136

w¹¹¹⁸; P{GD11869}v30136/CyO

v341707

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00814}attP40/CyO-GFP

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (53)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029157

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029157

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42986

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: ssh MAP-kinase-phosphatase

(Uemura and Niwa, 2001.3.5)

Source for database merge of

Source for merge of: MAP-kinase-phosphatase CG6238

(Bayraktaroglu, 2000.8.7)

Source for merge of: l(3)01207 ssh

(Niwa et al., 2002)

Source for merge of: ssh Mkph

(Moses, 2002.3.18)

Source for merge of: MAP-kinase-phosphatase ssh

(FlyBase, 1996-)

Additional comments

Source for merge of MAP-kinase-phosphatase ssh was sequence comparison ( date:010405 ).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

ssh plays a critical role in controlling actin dynamics and thus reactivating cofilin in cellular and developmental contexts.

(Niwa et al., 2002)

ssh regulates cytoskeletal reorganization.

(Niwa et al., 2001)

Strong mutations in ssh result in larval lethality. Adult escapers of hypomorphic alleles and mitotic clones of strong alleles show kinked and/or branched bristles and wing hairs.

(Niwa et al., 2001)

Inactivates rl kinase.

(Niwa et al., 2000)

Strong mutations caused larval lethality. Escaper adults of hypomorphic alleles and mitotic clones of strong ones exhibited kinked and/or branched bristles and wing hairs due to disruption of actin bundles.

(Niwa et al., 2000)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. ssh gene expression during oogenesis is not critical to embryonic development, but the gene function may be essential for fertilisation and/or completion of meiosis.

(Perrimon et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42986

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q6NN85

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029157

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029157

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ssh_1
ssh_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6238

Flygut - An atlas of the Drosophila adult midgut

FBgn0029157

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42986

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034442

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6238

modMine - A data warehouse for the modENCODE project

FBgn0029157

Linkouts

BioGRID - A database of protein and genetic interactions.

67907

DroID - A comprehensive database of gene and protein interactions.

FBgn0029157

DRSC - Results frm RNAi screens

FBgn0029157

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029157

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6238-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0029157

FlyMine - An integrated database for Drosophila genomics

FBgn0029157

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

42986

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG6238

(Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Derré et al., 2007, Montana and Littleton, 2006, Rogers et al., 2003, Morrison et al., 2000)

MKP

(Merabet et al., 2002, Niwa et al., 2000)

Mkph

(Uemura, 2000.2.7)

SSH

(Park et al., 2017, Barišić et al., 2011, von Blume et al., 2009)

Ssh

(Hatzihristidis et al., 2015, Muyskens and Guillemin, 2011, Jovceva et al., 2007, Baum, 2002)

l(3)01207

(Spradling et al., 1999, Russell et al., 1998, Perrimon et al., 1996, BDGP Project Members, 1994-1999)

Name Synonyms

MAP-kinase-phosphatase

MKP-like

(Morrison et al., 2000)

Slingshot

(Gupta et al., 2014, Lin et al., 2012, Fauvarque and Williams, 2011, D'Ambrosio and Vale, 2010, Serio et al., 2010, Pham et al., 2008, Sese et al., 2006, Rogers et al., 2003)

slingshot

(Deivasigamani et al., 2014, Schottenfeld-Roames et al., 2014, Sathyanarayanan et al., 2008, Bakal et al., 2007, Corrigall et al., 2007, Dilks and DiNardo, 2007, Iwasa and Mullins, 2007, Jovceva et al., 2007, Menzel et al., 2007, Montana and Littleton, 2006, Chen et al., 2005)

Secondary FlyBase IDs

FBgn0010740
FBgn0039253
FBgn0041200

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (109)

Report Sections

Open Close