FlyBase Gene Report: Dmel\eEF1γ

General Information

Symbol

Dmel\eEF1γ

Species

D. melanogaster

Name

eukaryotic translation elongation factor 1 gamma

Annotation Symbol

CG11901

Feature Type

protein_coding_gene

FlyBase ID

FBgn0029176

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

eukaryotic translation elongation factor 1 gamma (eEF1γ) encodes a protein that associates with other EF1 subunits (α/β/δ). It regulates organelle movement along microtubules. It binds tubulin and the protein kinase encoded by Doa, which in turns phosphorylates the product of eEF1γ. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Ef1gamma, Ef1γ

Key Links

Genomic Location

Cytogenetic map

99A1-99A1

Sequence location

3R:29,220,172..29,222,109 [+]

Recombination map

3-98

RefSeq locus

NT_033777 REGION:29220172..29222109

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables microtubule binding

inferred from direct assay

(Serpinskaya et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables translation elongation factor activity

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from electronic annotation with InterPro:IPR001662, InterPro:IPR036433

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in regulation of organelle transport along microtubule

inferred from mutant phenotype

(Serpinskaya et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in translational elongation

inferred from biological aspect of ancestor with PANTHER:PTN000136176

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from electronic annotation with InterPro:IPR001662, InterPro:IPR036433

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Fan et al., 2010)

located_in nucleus

inferred from direct assay

(Fan et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000136176

(GO Reference Genome Project, 2011-)

part_of eukaryotic translation elongation factor 1 complex

inferred from sequence or structural similarity

(Lasko, 2000)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000136176

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION ELONGATION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION ELONGATION FACTORS -: Cytoplasmic (or eukaryotic) translation elongation factors function in the chain elongation stage of ribosome-mediated polypeptide synthesis in the cytoplasm. (Adapted from FBrf0233552).

Protein Function (UniProtKB)

Microtubule binding protein involved in regulation of microtubule-based transport (PubMed:24163433). Probably plays a role in anchoring the EF-1 complex to other cellular components (By similarity). Probably involved in formation and/or development of mechanosensory organs during metamorphosis (PubMed:38264934). Required for cellular and organismal viability (PubMed:19841092). Not essential for the innate immune response to bacterial infection (PubMed:19841092).

(UniProt, Q9NJH0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eEF1γ for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9NJH0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.50

Gene model reviewed during 6.01

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eEF1γ-RA

FBtr0085393

NM_143743

1662

431

eEF1γ-RB

FBtr0085392

NM_170401

1685

431

eEF1γ-RC

FBtr0302442

NM_001202379

1689

431

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

eEF1γ-PA

FBpp0084762

49.0

431

7.10

FBpp0084761

49.0

431

7.10

FBpp0291632

49.0

431

7.10

Q9NJH0

NP_001189308

ADV37398

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

431 aa isoforms: eEF1γ-PA, eEF1γ-PB, eEF1γ-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with microtubules (PubMed:24163433). May interact with BicDR; the interaction is probably indirect (PubMed:38264934). Interacts (via C-terminus) with Doa; the interaction is probably direct, is transient and leads to phosphorylation of eEF1gamma by Doa (PubMed:19841092). EF-1 is composed of four subunits: alpha, beta, delta, and gamma.

(UniProt, Q9NJH0)

Post Translational Modification

Phosphorylated on Ser-294 by LAMMER kinases, including Doa (PubMed:19841092). Phosphorylation on Ser-294 by Doa is required for negative regulation of microtubule-based transport (PubMed:24163433).

(UniProt, Q9NJH0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9NJH0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eEF1γ using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ef1gamma_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.10

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Fan et al., 2010)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 12 -- 14

embryonic/larval midgut

Comment: enriched in midgut

(Fan et al., 2010)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Ef1γ expression is described as ubiquitous but dynamic during embryogenesis. In precellular blastoderm embryos, there is a anterior-to-posterior gradient. In cellular blastoderm embryos, there is enrichment in the ventral part of the embryo and at the posterior pole. In stage 12 and again at stage 13/14, particularly high expression levels are seen in the midgut.

(Fan et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Fan et al., 2010)

embryonic stage 12 -- 14

embryonic/larval midgut

(Fan et al., 2010)

embryonic stage 13 -- 14

larval ventral nerve cord

(Fan et al., 2010)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Ef1γ expression is described as ubiquitous but dynamic during embryogenesis. By stage 8, the protein is enriched in the developing ventral neurogenic region. In stage 12, particularly high expression levels are seen in the midgut. By stage 13/14, enrichment is seen in the ventral nerve cord in addition to the midgut.

(Fan et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Fan et al., 2010)

located_in nucleus

inferred from direct assay

(Fan et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eEF1γ in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eEF1γ

View exonic expression by tissue for Dmel\eEF1γ

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029176

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44791/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029176

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029176

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029176

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11901

Flygut - An atlas of the Drosophila adult midgut

FBgn0029176

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eEF1γ

Transgenic constructs containing regulatory region of eEF1γ

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)3450

(Marygold et al., 2007)

Df(3R)ED6310

(Marygold et al., 2007)

Df(3R)Exel6212

(Fan et al., 2010, Marygold et al., 2007, Ryder, 2004.4.26)

Gene not disrupted in

Df(3R)ED6310

(Ryder and Roote, 2004.11.10)

Df(3R)ex15C

(Sun et al., 2015)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | recessive | somatic clone

eEF1γ^A70

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eEF1γ^GD7427

lethal, with Scer\GAL4^pnr-MD237

eEF1γ^GD7427

lethal (with Df(3R)Exel6212)

lethal (with eEF1γ^A70)

eEF1γ^GE29510

lethal (with eEF1γ^GE29510)

eEF1γ^A70

lethal | recessive

eEF1γ^GE29510

lethal - all die before end of larval stage | recessive

eEF1γ^A70

lethal - all die before end of pupal stage | recessive

eEF1γ^A28

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eEF1γ^GD7427

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

eEF1γ^GD7427

viable

viable, with Scer\GAL4^tin.CΔ4

eEF1γ^GD7427

viable (with Df(3R)Exel6212)

eEF1γ^c01148

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage | recessive

eEF1γ^A28

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo

eEF1γ^GD7427

decreased cell number | third instar larval stage, with Scer\GAL4^repo

eEF1γ^GD7427

some die during larval stage | recessive

eEF1γ^A70

some die during pupal stage | recessive

eEF1γ^A28

visible, with Scer\GAL4^GMR.PF

visible | adult stage | dominant

visible | recessive

wild-type

eEF1γ^A15

Phenotype manifest in

Allele

anterior scutellar bristle | increased number

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

eEF1γ^GD7427

eye

eye, with Scer\GAL4^GMR.PF

eye | somatic clone

eEF1γ^A70

eye disc | third instar larval stage, with Scer\GAL4^repo

eEF1γ^GD7427

glial cell | third instar larval stage, with Scer\GAL4^repo

eEF1γ^GD7427

ommatidium, with Scer\GAL4^GMR.PF

posterior scutellar bristle

sarcomere, with Scer\GAL4^Mef2.PR

eEF1γ^GD7427

scutellar bristle | ectopic

wing blade posterior compartment, with Scer\GAL4^en-e16E

eEF1γ^UY752

Z disc, with Scer\GAL4^Mef2.PR

eEF1γ^GD7427

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\EEF1G

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Eef1g

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Eef1g

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\eef1g

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\eef1g

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\eef-1G

13 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (32)

Agam\AgaP_AGAP000883

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (29)

Atha\AT1G09640

12 of 13

Yes

Atha\AT1G57720

12 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\CAM1

12 of 13

Yes

Scer\TEF4

12 of 13

Yes

Scer\YGR201C

5 of 13

Yes

Scer\GTT2

2 of 13

Scer\URE2

2 of 13

Scer\GTT1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\SPAC29A4.02c

10 of 12

Yes

Spom\SPCC1183.02

5 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (8)

Ecol\gstA

2 of 11

Yes

Ecol\gstB

2 of 11

Yes

Ecol\yfcG

2 of 11

Yes

Ecol\yibF

2 of 11

Yes

Ecol\yncG

2 of 11

Yes

Ecol\sspA

1 of 11

Ecol\yfcF

1 of 11

Ecol\yghU

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:eEF1γ. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (42)

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EEF1G; eukaryotic translation elongation factor 1 gamma

14 of 14

130593

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eEF1γ - Chd64

experimental knowledge based

(Guruharsha et al., 2011)

eEF1γ - Doa

two hybrid

(Fan et al., 2010)

eEF1γ - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eEF1γ - Tcs3

experimental knowledge based

(Guruharsha et al., 2011)

eEF1γ - cPges

experimental knowledge based

(Guruharsha et al., 2011)

eEF1γ - capt

experimental knowledge based

(Guruharsha et al., 2011)

eEF1γ - eEF1δ

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9NJH0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

69280

DroID - A comprehensive database of gene and protein interactions.

FBgn0029176

MIST (protein-protein) - An integrated Molecular Interaction Database

44791

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Eukaryotic Translation Elongation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-98

Cytogenetic map

99A1-99A1

Sequence location

3R:29,220,172..29,222,109 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99A1-99A1

Limits computationally determined from genome sequence between P{PZ}l(3)04708⁰⁴⁷⁰⁸ and P{PZ}l(3)06743⁰⁶⁷⁴³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99A-99A

(Chaney, 1999.5.4)

Experimentally Determined Recombination Data

Location

3-98

(FlyBase, 2016)

3-94.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

40965

w^*; eEF1γ^A15

40962

w^*; eEF1γ^A42/TM6B, Tb¹

40963

w^*; eEF1γ^A70/TM6B, Tb¹

40964

w^*; eEF1γ^A28/TM6B, Tb¹

99884

w^*; P{UAS-eEF1γ.WT}1-4; eEF1γ^A70

31811

w¹¹¹⁸; P{EP}eEF1γ^G16379/TM6C, Sb¹

97678

y¹ w^*; TI{CRIMIC.TG4.0}eEF1γ^{CR02908-TG4.0}

99690

w^*; P{UAS-eEF1γ.S294A}25; eEF1γ^A70/TM6B, Tb¹

11901R-2

11901R-3

v100693

v31589

w¹¹¹⁸; P{GD7427}v31589

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (542)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029176

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029176

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Fan et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Duplicate transcripts identified and eliminated during the migration of annotations from the release 5 genome assembly to the release 6 assembly.

(FlyBase Genome Annotators, 2014)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ef1γ CG11901

(Bayraktaroglu, 2000.8.7)

Additional comments

FlyBase curator comment: one insertion in the "e00972" Exelixis line (PBac{RB}e00972) was originally assigned to the Ef1γ gene in FBrf0179797, resulting in the "Ef1γ^e00972" (FBal0160512) allele. However, FBrf0184340 shows that this was incorrect, and the PBac{RB}e00972 insertion is not within a transcription unit.

(Bloomington Drosophila Stock Center, 2004.11.6)

Nomenclature History

Source for database identify of

Source for identity of: eEF1γ Ef1γ

(Marygold et al., 2017)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG11901

(Kolkhof et al., 2017, Marygold et al., 2017, Hosono et al., 2015, Bonn et al., 2013, Cammarato et al., 2011, Fan et al., 2010, Müller et al., 2010, Keleman et al., 2009.8.5, Hughes et al., 2008, Lai et al., 2007, Quinones-Coello, 2007, Raisin et al., 2003, Ranson et al., 2001, Lasko, 2000)

EF1 γ

(Hsiao et al., 2014)

EF1γ

(Jejina et al., 2024, Avet-Rochex et al., 2010, Fan et al., 2010, Foley and O'Farrell, 2004)

Ef1Bggr

(Nakahara and Carthew, 2004)

Ef1gamma

(Dent et al., 2015, Kwong et al., 2015, Ashwal-Fluss et al., 2014, Mukherjee et al., 2014, Kwon et al., 2013, Kallappagoudar et al., 2010, Kong et al., 2010, Müller et al., 2010, Fisher et al., 2008, Quinones-Coello, 2007, Beller et al., 2006, Loop et al., 2004, Giot et al., 2003, Gorski et al., 2003)

Ef1y

(Bonn et al., 2013)

Ef1γ

(Tan et al., 2016, Tavares et al., 2015, FlyBase Genome Annotators, 2014, Fan et al., 2010, Marygold et al., 2007, Stuart et al., 2007)

eEF1gamma

(Pang et al., 2021, Gene Disruption Project members, 2018-)

eEF1γ

(Beghelli et al., 2024, Li et al., 2024, Evans et al., 2021, Marygold et al., 2017, Lasko, 2000)

Name Synonyms

Ef1gamma

(Cammarato et al., 2011)

Ef1γ

eukaryotic translation elongation factor 1 gamma

Secondary FlyBase IDs

FBgn0039652

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44791

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9NJH0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9NJH0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9NJH0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029176

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44791/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029176

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029176

FlyMine - An integrated database for Drosophila genomics

FBgn0029176

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11901

MARRVEL_MODEL - MARRVEL (model organism gene)

44791

Linkouts

BioGRID - A database of protein and genetic interactions.

69280

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029176

DroID - A comprehensive database of gene and protein interactions.

FBgn0029176

DRSC - Results frm RNAi screens

FBgn0029176

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ef1gamma_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029176

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0029176

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11901

Flygut - An atlas of the Drosophila adult midgut

FBgn0029176

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0029176

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007229

MIST (protein-protein) - An integrated Molecular Interaction Database

44791

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Eukaryotic Translation Elongation

References (90)

The following external sites may use different assemblies or annotations than FlyBase.

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