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Gene Dmel\Aos1

General Information
Symbol	Dmel\Aos1	Species	D. melanogaster
Name	Aos1	Annotation symbol	CG12276
Feature type	protein_coding_gene	FlyBase ID	FBgn0029512
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	SAE1, DmAos1, DmSae1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87B10-87B10	Sequence location	3R:8,258,255..8,259,544 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Aos1 is referred to in FlyBase by the symbol Dmel\Aos1 (CG12276, FBgn0029512). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: protein sumoylation; positive regulation of NF-kappaB transcription factor activity; regulation of mitotic cell cycle; neurogenesis. 13 alleles are reported. The phenotypes of these alleles are annotated with: eye disc; eye; adult head; mesothoracic tergum; imaginal disc. It has one annotated transcript and one annotated polypeptide. Protein features are: Molybdenum cofactor biosynthesis, MoeB; NAD(P)-binding domain; UBA/THIF-type NAD/FAD binding fold; Ubiquitin/SUMO-activating enzyme E1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1629655_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Aos1. Gene sequence location is 3R:8258255..8259544.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87B10-87B10   Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Aos1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Aos1-RA FBtr0082593  NM_141941   1167   337
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Aos1-PA   FBpp0082065   37.7   337   5.08     NP_650198   AAF54815
Additional Polypeptide Data & Comments
Reported size (kDa)	337 (aa) (Bhaskar et al., 2000)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin/SUMO-activating enzyme E1 (IPR000011) UBA/THIF-type NAD/FAD binding fold (IPR000594) Molybdenum cofactor biosynthesis, MoeB (IPR009036) NAD(P)-binding domain (IPR016040)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392705   AA439334   AA735880   AA942622   AA979277   AA979278   AE014297 AAF54815   AF193554 AAF25198   AF218863 AAF31703   AI257455   AI259689   AI944694   AW943192   AY069589 AAL39734   BF491828   BI165872   BI166515   BI237896   BI238202   BI367707   BI368173   BI481701   BI635070   BI640102   BP556008   BP559711   BP559712   BQ103502   CO180625   CO264359   CO296312   CZ470451   EC064410   EC201847   EC232942   EC266935   EV575945   EV592259
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KSM5 Q9VG70 Q7KJV5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium foregut primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) Malpighian tubule primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Smith et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1070.95) Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland  512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary  (941.6) Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1070.95) Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland  512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary  (941.6) Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1070.95 Larval Midgut    140.3 Larval Hindgut    191.1 Larval Malpighian Tubules    287.4 Larval Fat Body    206.7 Larval Salivary Gland    512 Larval Trachea    484.05 Larval Carcass    305.05 Adult Head    172 Adult Eye    188.575 Adult Brain    178.7 Adult Thoracic-Abdominal Ganglion    211 Adult Crop    194 Adult Midgut    177.7 Adult Hindgut    156.8 Adult Malpighian Tubules    209.4 Adult Fat Body    159 Adult Salivary Gland    151.2 Adult Heart    181.875 Adult VirginFemale Spermatheca    155.2 Adult InseminatedFemale Spermatheca    183.5 Adult Ovary    941.6 Adult Testis    188.2 Adult Male Accessory Gland    283.6 Adult Carcass    179.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    172   NA  Eye    188.575   NA  Brain    178.7   1070.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    211   NA  Crop    194   140.3  Midgut    177.7   191.1  Hindgut    156.8   287.4  Malpighian Tubules    209.4   206.7  Fat Body    159   512  Salivary Gland    151.2   NA  Heart    181.875   484.05  Trachea    NA   NA  VirginFemale Spermatheca    155.2   NA  InseminatedFemale Spermatheca    183.5   NA  Ovary    941.6   NA  Testis    188.2   NA  Male Accessory Gland    283.6   305.05  Carcass    179.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    117 ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (71) central nervous system, larvae L3  (75) central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering  (48) carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  (117) ovary, mated 4-day female  (73) testis, mated 4-day male  (46) accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  (117) ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    117 ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    117 ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (71) central nervous system, larvae L3  (75) central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering  (48) carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  (117) ovary, mated 4-day female  (73) testis, mated 4-day male  (46) accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  117 ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    71 central nervous system, larvae L3    75 central nervous system, pupae P8    20 head, virgin 1-day female    7 head, virgin 4-day female    6 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    6 head, mated 20-day female    6 head, mated 1-day male    10 head, mated 4-day male    9 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    10 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    14 carcass, larvae L3 wandering    48 carcass, 1-day adult    6 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    117 ovary, mated 4-day female    73 testis, mated 4-day male    46 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    177 embryo 04-06hr    158 embryo 06-08hr    104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (177) embryo 04-06hr  (158) embryo 06-08hr  (104) embryo 08-10hr  (78) embryo 10-12hr  (54) embryo 12-14hr  (29) embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2  (31) larva L3 12hr old    23 larva L3 puffstage 1-2  (35) larva L3 puffstage 3-6  (41) larva L3 puffstage 7-9  (54) white prepupae new  (48) white prepupae 12hr  (53) white prepupae 24hr  (58) pupae 2d postWPP  (27) pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day  (38) adult female 05day  (83) adult female 30day  (84) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (177) embryo 04-06hr  (158) embryo 06-08hr  104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    177 embryo 04-06hr    158 embryo 06-08hr    104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    177 embryo 04-06hr    158 embryo 06-08hr    104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (177) embryo 04-06hr  (158) embryo 06-08hr  (104) embryo 08-10hr  (78) embryo 10-12hr  (54) embryo 12-14hr  29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2  31 larva L3 12hr old    23 larva L3 puffstage 1-2  (35) larva L3 puffstage 3-6  (41) larva L3 puffstage 7-9  (54) white prepupae new  (48) white prepupae 12hr  (53) white prepupae 24hr  (58) pupae 2d postWPP  27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day  (38) adult female 05day  (83) adult female 30day  (84) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (177) embryo 04-06hr  (158) embryo 06-08hr  104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    177 embryo 04-06hr    158 embryo 06-08hr    104 embryo 08-10hr    78 embryo 10-12hr    54 embryo 12-14hr    29 embryo 14-16hr    18 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    8 larva L1    20 larva L2    31 larva L3 12hr old    23 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    41 larva L3 puffstage 7-9    54 white prepupae new    48 white prepupae 12hr    53 white prepupae 24hr    58 pupae 2d postWPP    27 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    18 adult male 30day    15 adult female 01day    38 adult female 05day    83 adult female 30day    84 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    157 Schneider line 2 Sg4    173 embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1    117 embryonic S3    91 leg disc CME L1    107 wing disc CME-W2    107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21    133 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    118 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    131 mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (157) Schneider line 2 Sg4  (173) embryonic 1182-4H  (68) embryonic GM2  (68) embryonic Kc167  (87) embryonic S1  (117) embryonic S3  (91) leg disc CME L1  (107) wing disc CME-W2  (107) wing disc ML-DmD8  (85) wing disc ML-DmD9  (70) wing disc ML-DmD16-c3  (84) wing disc ML-DmD21  (133) wing disc ML-DmD32  (62) haltere disc ML-DmD17-c3  (118) eye-antennal disc ML-DmD11  (74) antennal disc ML-DmD20-c5  (131) mixed discs ML-DmD4-c1  (98) CNS ML-DmBG1-c1  (76) CNS ML-DmBG2-c2  (93) tumorous blood cells mbn2  (58) ovary fGS/OSS  (62) ovary OSC  (57) ovary OSS  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (157) Schneider line 2 Sg4  (173) embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1  (117) embryonic S3    91 leg disc CME L1  107 wing disc CME-W2  107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21  (133) wing disc ML-DmD32    62 haltere disc ML-DmD17-c3  (118) eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5  (131) mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    157 Schneider line 2 Sg4    173 embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1    117 embryonic S3    91 leg disc CME L1    107 wing disc CME-W2    107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21    133 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    118 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    131 mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    157 Schneider line 2 Sg4    173 embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1    117 embryonic S3    91 leg disc CME L1    107 wing disc CME-W2    107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21    133 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    118 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    131 mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (157) Schneider line 2 Sg4  (173) embryonic 1182-4H  (68) embryonic GM2  (68) embryonic Kc167  (87) embryonic S1  (117) embryonic S3  (91) leg disc CME L1  (107) wing disc CME-W2  (107) wing disc ML-DmD8  (85) wing disc ML-DmD9  (70) wing disc ML-DmD16-c3  (84) wing disc ML-DmD21  (133) wing disc ML-DmD32  (62) haltere disc ML-DmD17-c3  (118) eye-antennal disc ML-DmD11  (74) antennal disc ML-DmD20-c5  (131) mixed discs ML-DmD4-c1  (98) CNS ML-DmBG1-c1  (76) CNS ML-DmBG2-c2  (93) tumorous blood cells mbn2  (58) ovary fGS/OSS  (62) ovary OSC  (57) ovary OSS  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (157) Schneider line 2 Sg4  (173) embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1  117 embryonic S3    91 leg disc CME L1  107 wing disc CME-W2  107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21  133 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3  118 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5  131 mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    157 Schneider line 2 Sg4    173 embryonic 1182-4H    68 embryonic GM2    68 embryonic Kc167    87 embryonic S1    117 embryonic S3    91 leg disc CME L1    107 wing disc CME-W2    107 wing disc ML-DmD8    85 wing disc ML-DmD9    70 wing disc ML-DmD16-c3    84 wing disc ML-DmD21    133 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    118 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    131 mixed discs ML-DmD4-c1    98 CNS ML-DmBG1-c1    76 CNS ML-DmBG2-c2    93 tumorous blood cells mbn2    58 ovary fGS/OSS    62 ovary OSC    57 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult  (38) Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3  (40) Cadmium 50 mM 48 hrs, 4-day adult  (30) Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult  (32) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (60) Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult  (46) Paraquat 10 mM 48 hrs, 4-day adult  (40) Rotenone 2 μg 12 hrs, larvae L3  (28) Rotenone 8 μg 12 hrs, larvae L3  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult  (38) Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3  (40) Cadmium 50 mM 48 hrs, 4-day adult  30 Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult  32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (60) Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult  (46) Paraquat 10 mM 48 hrs, 4-day adult  (40) Rotenone 2 μg 12 hrs, larvae L3  28 Rotenone 8 μg 12 hrs, larvae L3  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    10 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    40 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    21 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    16 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    40 Rotenone 2 μg 12 hrs, larvae L3    28 Rotenone 8 μg 12 hrs, larvae L3    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD33652 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029512 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12276-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Aos1 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | somatic clone | third instar larval stage Aos1c06048 cell lethal | somatic clone - Minute background | third instar larval stage Aos1c06048 lethal Aos1GD16818 lethal | P-stage Aos1c06048 lethal | P-stage (with Df(3R)ED5554) Aos1c06048 lethal | P-stage (with Df(3R)ED5591) Aos1c06048 lethal | pupal stage, with Scer\GAL4pnr-MD237 Aos1GD8542 Aos1GD16818 lethal | third instar larval stage Aos1N1 Aos1N2 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Aos1GD16818 Other Phenotypes Allele decreased cell number | somatic clone | third instar larval stage Aos1c06048 developmental rate defective Aos1c06048 developmental rate defective | wandering third instar larval stage Aos1N1 Aos1N2 increased cell death | somatic clone | third instar larval stage Aos1c06048 increased cell death | somatic clone - Minute background | third instar larval stage Aos1c06048 melanotic mass phenotype | third instar larval stage Aos1c06048 mitotic cell cycle defective | somatic clone | third instar larval stage Aos1c06048 neuroanatomy defective | larval stage Aos1GD16818 visible, with Scer\GAL4ey.PU Aos1NIG.12276R visible, with Scer\GAL4pnr-MD237 Aos1GD16818 Phenotype manifest in Allele adult head, with Scer\GAL4ey.PU Aos1NIG.12276R embryonic/larval neuroblast | larval stage Aos1GD16818 embryonic/larval optic lobe | third instar larval stage Aos1N1 Aos1N2 eye, with Scer\GAL4ey.PU Aos1NIG.12276R eye disc, with Scer\GAL4ey.PU Aos1NIG.12276R ganglion mother cell | larval stage Aos1GD16818 imaginal disc (with Df(3R)ED5554) Aos1c06048 imaginal disc (with Df(3R)ED5591) Aos1c06048 imaginal disc | larval stage Aos1c06048 imaginal disc | somatic clone | third instar larval stage Aos1c06048 imaginal disc | third instar larval stage Aos1N1 Aos1N2 mesothoracic tergum, with Scer\GAL4pnr-MD237 Aos1GD16818
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Aos1 Class Mutagen Stocks Known lesion Aos1c06048 amorphic allele - molecular evidence piggyBac transposase 2 Yes Aos1N1 amorphic allele - molecular evidence ends-out gene targeting 0 Yes Aos1N2 amorphic allele - molecular evidence ends-out gene targeting 0 Yes
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Aos1 Class Mutagen Stocks Known lesion Aos1GD16818 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Aos1GD8542 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Aos1GL00493 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Aos1HM05183 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Aos1+t2.9 in vitro construct - genomic fragment 0 Yes Aos1Act5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Aos1NIG.12276R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Aos1Scer\UAS.cKa in vitro construct - regulatory fusion 0 Yes Aos1t2 in vitro construct - genomic fragment 0 Yes Aos1VDRC.cUa 0 --
Aneuploid Aberrations
Disrupted in	Df(3R)ED5554 (Kanakousaki and Gibson, 2012) Df(3R)ED5591 (Kanakousaki and Gibson, 2012) Df(3R)Exel6164 (Ryder, 2004.4.26) Df(3R)Exel6165 (Ryder, 2004.4.26) Df(3R)Exel7316 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8542} NA P{GD16818} NA P{NIG.12276R} NA P{TRiP.GL00493} NA P{TRiP.HM05183} NA P{UAS-Aos1.K} NA characterization construct P{Aos1.+2.9} NA P{Pros25+t2} NA P{VDRC.Aos.U} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Aos1c06048 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Uba2 (Shih et al., 2002)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of NF-kappaB transcription factor activity inferred from direct assay (Bhaskar et al., 2000) protein sumoylation inferred from mutant phenotype (Kanakousaki and Gibson, 2012) regulation of mitotic cell cycle inferred from mutant phenotype (Kanakousaki and Gibson, 2012)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Smith et al., 2004)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
small protein activating enzyme activity inferred from electronic annotation with InterPro:IPR000011 (FlyBase Curators et al., 2004-) SUMO activating enzyme activity inferred from sequence or structural similarity (Bhaskar et al., 2000) non-traceable author statement (Bhaskar, 1999.12.27)
Biological Process
CV term Evidence References
protein sumoylation inferred from sequence or structural similarity (Bhaskar et al., 2000) SMT3-dependent protein catabolic process non-traceable author statement (Bhaskar, 1999.12.27)
Cellular Component
CV term Evidence References
SUMO activating enzyme complex inferred from sequence or structural similarity (Bhaskar et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Aos1 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - AnxB10 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Aos1 - CG15717 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - CG3887 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - CG7675 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - CG8745 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - P32 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosα7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Prosβ7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Tapδ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Torsin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Aos1 - Uba2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Aos1 - Ubc12 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Aos1 - lwr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Aos1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66630 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029512 DroID - A comprehensive database of gene and protein interactions. • FBgn0029512 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41532
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VMG1H Dipteran inclusive ortholog search EOG62V8R2 Insect inclusive ortholog search EOG6HHMZ5 Arthropod inclusive ortholog search EOG6NVX23 Metazoa inclusive ortholog search EOG6QP0Q2
Orthologs in Drosophila Species (EOG6VMG1H)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Aos1     Drosophila simulans   Dsim\GD20565 Y   Drosophila sechellia   Dsec\GM26007 Y   Drosophila erecta   Dere\GG17124 Y   Drosophila yakuba   Dyak\GE24516 Y   Drosophila ananassae   Dana\GF17042 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11527 Y   Drosophila persimilis   Dper\GL27174 Y   Drosophila willistoni   Dwil\GK12842 Y   Drosophila virilis   Dvir\GJ22581 Y   Drosophila mojavensis   Dmoj\GI10362 Y   Drosophila grimshawi   Dgri\GH23591 Y
Orthologs in non-Drosophila Dipterans (EOG62V8R2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000091   Anopheles gambiae Malaria mosquito  Agam\AGAP004459   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013962
Orthologs in non-Dipteran Insects (EOG6HHMZ5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49813   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005172   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11072   Atta cephalotes Leafcutter ant  Acep\ACEP19968   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14921   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15740   Linepithema humile Argentine ant  Lhum\LH17939   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25938   Solenopsis invicta Red fire ant  Sinv\SINV11946   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002408   Bombyx mori Silkmoth  Bmor\BGIBMGA002755   Pediculus humanus Human body louse  Phum\PHUM428030   Tribolium castaneum Red flour beetle  Tcas\TC006385
Orthologs in non-Insect Arthropods (EOG6NVX23)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_210457   Ixodes scapularis Deer tick  Isca\ISCW012441
Orthologs in non-Arthropod Metazoa (EOG6QP0Q2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\aos-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009761gn   Danio rerio Zebrafish  Drer\sae1   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000000161   Mus musculus House mouse  Mmus\ENSMUSG00000052833   Rattus norvegicus Norway rat  Rnor\Sae1   Homo sapiens Human  Hsap\SAE1
Human Orthologs (1)
Gene OMIM HGNC Hsap\SAE1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20565 Drosophila sechellia Dsec\GM26007 Drosophila erecta Dere\GG17124 Drosophila yakuba Dyak\GE24516 Drosophila ananassae Dana\GF17042 Drosophila pseudoobscura pseudoobscura Dpse\GA11527 Drosophila persimilis Dper\GL27174 Drosophila willistoni Dwil\GK12842 Drosophila virilis Dvir\GJ22581 Drosophila mojavensis Dmoj\GI10362 Drosophila grimshawi Dgri\GH23591
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	36074 y1 sc* v1; P{TRiP.GL00493}attP2 17744 w1118; PBac{PB}Aos1c06048/TM6B, Tb1 28972 y1 v1; P{TRiP.HM05183}attP2/TM3, Sb1
Harvard	- PBac{PB}Aos1c06048
VDRC	v18528 w1118 P{GD8542}v18528 v47256 w1118; P{GD16818}v47256 v47257 w1118; P{GD16818}v47257/TM3
Genomic Clones ( 1 )
	BACR28I14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 32 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD33652
Other clones	DPiM_LD33652
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT28746 GM10027 LD11809 LD13875 LD26944 LD33653 LP03158 LP05830 RE05858 RE06666 RE34224 RE34601 RE53513 RE54092 RE64294 SD16308 SD22888
Other clones	30189 82378 82486 562028 bs04c10 Dmel_dig_010376_0603_3710 Dmel_neb_013673_0688_0618 EC02931 EK138743 EK213345 FGM238G06 FGM322D12 UUGC0909
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0029512 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41532
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Aos1 SAE1 CG12276 (Bayraktaroglu, 2000.5.22)
Additional comments
Other Comments
	Expression is enriched in embryonic gonads. (Shigenobu et al., 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141941 RefSeq (Proteins) • NP_650198 DDBJ / EMBL / GenBank • AA439334 AA735880 AA979277 AAF25198 AAF31703 AAF54815 AAL39734 AF193554 AF218863 AI944694 AW943192 AY069589 CZ470451 Entrez Gene - A searchable database of RefSeq genes. • 41532 UniProtKB/TrEMBL • Q7KJV5 Q7KSM5 Q9VG70
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin/SUMO-activating enzyme E1 (IPR000011) UBA/THIF-type NAD/FAD binding fold (IPR000594) Molybdenum cofactor biosynthesis, MoeB (IPR009036) NAD(P)-binding domain (IPR016040) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD33652
Linkouts
	BioGRID - A database of protein and genetic interactions • 66630 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0029512 DroID - A comprehensive database of gene and protein interactions. • FBgn0029512 DRSC - Results from RNAi screens. • FBgn0029512 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029512 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12276-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0029512 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41532 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41532 modMine - Data generated by the modENCODE project. • FBgn0029512
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	Aos (Neuburger et al., 2006) Aos1 (Shigenobu et al., 2006, Geng and MacDonald, 2007, Hashiyama et al., 2009, Avet-Rochex et al., 2010, Paddibhatla et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Kanakousaki and Gibson, 2012) CG12276 (Gonzalez-Aguero et al., 2005, Bayraktaroglu, 2000.5.22, Proschel et al., 2006, Perkins et al., 2009, Ni et al., 2011.7.19) DmAos1 (Shih et al., 2002, Long and Griffith, 2000, Shih, 1999.10.8) Dmaos1 (Shih et al., 2000) DmSae1 (Smith et al., 2001) DmSAE1 (Bhaskar et al., 2000) SAE1 (Bhaskar et al., 2002, Smith et al., 2004, Bayraktaroglu, 2000.5.22, Bhaskar, 1999.12.27, Nie et al., 2009) Sae1 (Smith et al., 2002)
Name Synonym	Aos1   Smt3 activating enzyme 1 (Bhaskar, 1999.12.27) Ubiquitin activating enzyme (Takahashi et al., 2001) Ubiquitin activating enzyme 1 (Varma and Mishra, 2011)
Secondary FlyBase IDs
	FBgn0038048 FBgn0040288
References ( 47 )
Generate a list of	All references relating to this gene ( 47 )
List References by type	Research paper  ( 18 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Kalamarz et al., 2012, Biol. Open 1(3): 161--172 Sumoylation is tumor-suppressive and confers proliferative quiescence to hematopoietic progenitors in Drosophila melanogaster larvae. [FBrf0220167] Kanakousaki and Gibson, 2012, Development 139(15): 2751--2762 A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development. [FBrf0218825] Varma and Mishra, 2011, J. Biosci., Bangalore 36(3): 439--459 Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster. [FBrf0214552] Show all research papers ...