FB2020_06, released Dec 22, 2020

Gene: Dmel\eIF3g1

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General Information
Symbol
Dmel\eIF3g1
Species
D. melanogaster
Name
eukaryotic translation initiation factor 3 subunit g1
Annotation Symbol
CG8636
Feature Type
protein_coding_gene
FlyBase ID
FBgn0029629
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

eIF3-S4, eIF3ga

Key Links
Genomic Location
Cytogenetic map
3A7-3A7
Sequence location
X:2,609,247..2,610,336 [-]
Recombination map

1-1

RefSeq locus
NC_004354 REGION:2609247..2610336
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from sequence or structural similarity
(Lasko, 2000)
enables translation initiation factor activity
inferred from electronic annotation with InterPro:IPR017334
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
inferred from sequence or structural similarity
(Lasko, 2000)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in translational initiation
inferred from sequence or structural similarity
(Lasko, 2000)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 3 complex
inferred from electronic annotation with InterPro:IPR017334
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
inferred from sequence or structural similarity
(Lasko, 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the eIF-3 subunit G family. (Q9W4X7)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Protein Function (UniProtKB)
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.
(UniProt, Q9W4X7)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\eIF3g1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF3g1-RA
FBtr0070446
NM_130655
1027
269
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
eIF3g1-PA
FBpp0070430
29.9
269
9.19
NP_570011
AAF45796
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, Q9W4X7)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3g1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
embryonic/larval visceral muscle
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3g1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF3g1
Transgenic constructs containing regulatory region of eIF3g1
Deletions and Duplications ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4pnr-MD237
lethal, with Scer\GAL4tin.CΔ4
lethal - all die during larval stage, with Scer\GAL4Mef2.PR
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\EIF3G
13 of 15
Yes
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Eif3g
12 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Eif3g
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\eif3g
12 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\eif3g
13 of 15
Yes
Yes
Drer\si:ch73-190f9.4
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\eif-3.G
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\EIF3G1
8 of 9
Yes
Yes
Atha\EIF3G2
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\TIF35
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\tif35
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190DBK )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila simulans
Dsim\GD24609
Drosophila sechellia
Dsec\GM18870
Drosophila erecta
Dere\GG12605
Drosophila yakuba
Dyak\GE16936
Drosophila ananassae
Dana\GF21942
Drosophila pseudoobscura pseudoobscura
Dpse\GA21226
Drosophila persimilis
Dper\GL12937
Drosophila willistoni
Dwil\GK16326
Drosophila virilis
Dvir\GJ15710
Drosophila mojavensis
Dmoj\GI16065
Drosophila grimshawi
Dgri\GH21559
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509N4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015181
Glossina morsitans
Tsetse fly
Gmor\GMOY010157
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09236
Mayetiola destructor
Hessian fly
Mdes\Mdes013640
Aedes aegypti
Yellow fever mosquito
Aaeg\eIF3-S4-2
Anopheles darlingi
American malaria mosquito
Adar\ADAC004168
Anopheles gambiae
Malaria mosquito
Agam\eIF3-S4
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015350
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0APY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000367
Danaus plexippus
Monarch butterfly
Dple\DPOGS215568-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016928
Apis florea
Little honeybee
Aflo\3348131572
Apis mellifera
Western honey bee
Amel\LOC408386
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10334
Bombus terrestris
Buff-tailed bumblebee
Bter\15213046
Linepithema humile
Argentine ant
Lhum\LH22528
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0004f6
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000311
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000315
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01659
Tribolium castaneum
Red flour beetle
Tcas\TC012449
Pediculus humanus
Human body louse
Phum\PHUM467340
Rhodnius prolixus
Kissing bug
Rpro\RPRC003600
Cimex lectularius
Bed bug
Clec\CLEC013420
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI008682
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004547
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06845
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AMZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003510
Ixodes scapularis
Black-legged tick
Isca\ISCW000203
Stegodyphus mimosarum
African social velvet spider
Smim\KFM69146.1
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_230293
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GSV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Eif3s4
Ciona intestinalis
Vase tunicate
Cint\LOC100178787
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
eIF3g2
9 of 10
CG1316
1 of 10
CG34354
1 of 10
elav
1 of 10
fne
1 of 10
Rbp9
1 of 10
Rox8
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF3G; eukaryotic translation initiation factor 3 subunit G
    13 of 15
    603913
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
        (UniProt, Q9W4X7 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-1

        Cytogenetic map
        3A7-3A7
        Sequence location
        X:2,609,247..2,610,336 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        3A7-3A7
        Limits computationally determined from genome sequence between P{EP}eghEP804 and P{EP}sggEP1576
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        1-1

        (FlyBase, 2016)

        1-1.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (6)
        Genomic Clones (13)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (279)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: eIF3ga CG8636

          (Majzoub et al., 2014)

          Source for identity of: eIF3g1 eIF3ga

          (Marygold et al., 2017)
          Source for database merge of
          Additional comments

          The CG8636 and CG10881 genes share at least 50% identity.

          (Langille and Clark, 2007)

          The CG10881 gene may have been derived from the CG8636 gene by retroposition.

          (Dai et al., 2006)
          Other Comments

          RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

          (Goshima et al., 2007)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 40 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (11)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (70)