FlyBase Gene Report: Dmel\eIF3g1

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).

Protein Function (UniProtKB)

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.

(UniProt, Q9W4X7)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\eIF3g1 or the JBrowse view of Dmel\eIF3g1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF3g1-RA

FBtr0070446

NM_130655

1027

269

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

eIF3g1-PA

FBpp0070430

29.9

269

9.19

NP_570011

AAF45796

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, Q9W4X7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W4X7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3g1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8636_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

translation initiation factor activity

inferred from electronic annotation with InterPro:IPR017334

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

eukaryotic translation initiation factor 3 complex

inferred from electronic annotation with InterPro:IPR017334

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Lasko, 2000)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

embryonic/larval visceral muscle

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3g1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF3g1

View exonic expression by tissue for Dmel\eIF3g1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029629

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029629

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8636-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8636

Flygut - An atlas of the Drosophila adult midgut

FBgn0029629

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\eIF3g1

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF3g1

Transgenic constructs containing regulatory region of eIF3g1

Deletions and Duplications ( 3 )

Disrupted in

Df(1)ED411

(Marygold et al., 2007)

Df(1)w258-11

(Marygold et al., 2007)

Duplicated in

Dp(1)weeb²

(Kemppainen et al., 2009)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

eIF3g1^GD13992

lethal, with Scer\GAL4^tin.CΔ4

eIF3g1^GD13992

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

eIF3g1^GD13992

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EIF3G

13 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eif3g

12 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Eif3g

12 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\eif3g

10 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\eif3g

13 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\eif-3.G

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\EIF3G1

8 of 9

Yes

Atha\EIF3G2

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TIF35

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\tif35

11 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190DBK )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21226

Drosophila persimilis

Dper\GL12937

Drosophila willistoni

Dwil\GK16326

Drosophila virilis

Dvir\GJ15710

Drosophila mojavensis

Dmoj\GI16065

Drosophila grimshawi

Dgri\GH21559

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509N4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015181

Glossina morsitans

Tsetse fly

Gmor\GMOY010157

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09236

Mayetiola destructor

Hessian fly

Mdes\Mdes013640

Aedes aegypti

Yellow fever mosquito

Aaeg\eIF3-S4-2

Anopheles darlingi

American malaria mosquito

Adar\ADAC004168

Anopheles gambiae

Malaria mosquito

Agam\eIF3-S4

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015350

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0APY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000367

Danaus plexippus

Monarch butterfly

Dple\DPOGS215568-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL016928

Apis florea

Little honeybee

Aflo\3348131572

Apis mellifera

Western honey bee

Amel\LOC408386

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10334

Bombus terrestris

Buff-tailed bumblebee

Bter\15213046

Linepithema humile

Argentine ant

Lhum\LH22528

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0004f6

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000311

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000315

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01659

Tribolium castaneum

Red flour beetle

Tcas\TC012449

Pediculus humanus

Human body louse

Phum\PHUM467340

Rhodnius prolixus

Kissing bug

Rpro\RPRC003600

Cimex lectularius

Bed bug

Clec\CLEC013420

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008682

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004547

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06845

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AMZ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003510

Ixodes scapularis

Black-legged tick

Isca\ISCW000203

Stegodyphus mimosarum

African social velvet spider

Smim\KFM69146.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230293

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GSV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Eif3s4

Ciona intestinalis

Vase tunicate

Cint\LOC100178787

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

9 of 10

1 of 10

1 of 10

1 of 10

1 of 10

fne

1 of 10

Rbp9

1 of 10

Rox8

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF3G; eukaryotic translation initiation factor 3 subunit G

13 of 15

603913

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eIF3g1 - Thor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3g1 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3g1 - eIF3a

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3b

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3c

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3e

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3f1

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3h

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3k

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3l

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - eIF3m

experimental knowledge based

(Guruharsha et al., 2011)

eIF3g1 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, Q9W4X7 )

Linkouts

BioGRID - A database of protein and genetic interactions.

57774

DroID - A comprehensive database of gene and protein interactions.

FBgn0029629

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31243

MIST (protein-protein) - An integrated Molecular Interaction Database

eIF3ga

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-1

Cytogenetic map

3A7-3A7

Sequence location

X:2,609,247..2,610,336 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3A7-3A7

Limits computationally determined from genome sequence between P{EP}egh^EP804 and P{EP}sgg^EP1576

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-1

(FlyBase, 2016)

1-1.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Bloomington

43243

y¹ sc^* v¹ sev²¹; P{TRiP.GLC01430}attP2

52344

y¹ eIF3g1^A w^* P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

FlyORF

F001643

M{UAS-eIF3g1.ORF}ZH-86Fb

F001055

M{UAS-eIF3g1.ORF.3xHA}ZH-86Fb

VDRC

v105325

P{KK110954}VIE-260B

v28937

w¹¹¹⁸; P{GD13992}v28937/TM3

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (279)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD24347

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029629

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029629

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31243

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: eIF3ga CG8636

(Majzoub et al., 2014)

Source for identity of: eIF3g1 eIF3ga

(Marygold et al., 2017)

Source for database merge of

Additional comments

The CG8636 and CG10881 genes share at least 50% identity.

(Langille and Clark, 2007)

The CG10881 gene may have been derived from the CG8636 gene by retroposition.

(Dai et al., 2006)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31243

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W4X7

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029629

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029629

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG8636_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8636

Flygut - An atlas of the Drosophila adult midgut

FBgn0029629

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31243

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012449

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8636

modMine - A data warehouse for the modENCODE project

FBgn0029629

Linkouts

BioGRID - A database of protein and genetic interactions.

57774

DroID - A comprehensive database of gene and protein interactions.

FBgn0029629

DRSC - Results frm RNAi screens

FBgn0029629

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0029629

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8636-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0029629

FlyMine - An integrated database for Drosophila genomics

FBgn0029629

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31243

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (protein-protein) - An integrated Molecular Interaction Database

eIF3ga

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

cg8636

(Lasko, 2000)

eIF s4

(Ducat et al., 2008)

eIF-3

(Goshima et al., 2007)

eIF-3ga

(Grobler et al., 2018)

eIF3-S4

(Haelterman et al., 2014, Olesnicky et al., 2014, Bonke et al., 2013, Guest et al., 2011, Somma et al., 2008, Lasko, 2000)

eIF3g

(Sen and Cox, 2016)

eIF3g1

(Marygold et al., 2017)

eIF3ga

(Brooks et al., 2015, Majzoub et al., 2014)

Name Synonyms

eIF3g paralogue a

eukaryotic translation initiation factor 3 subunit g1

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (66)

Report Sections

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