FlyBase Gene Report: Dmel\Grip

General Information

Symbol

Dmel\Grip

Species

D. melanogaster

Name

Glutamate receptor interacting protein

Annotation Symbol

CG14447

Feature Type

protein_coding_gene

FlyBase ID

FBgn0029830

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Glutamate receptor binding protein (Grip) encodes a protein involved in muscle attachment and synapse organization. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

dGRIP

Key Links

Genomic Location

Cytogenetic map

5D1-5D1

Sequence location

X:5,966,279..5,986,699 [+]

Recombination map

1-15

RefSeq locus

NC_004354 REGION:5966279..5986699

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:ed; FB:FBgn0000547

(Swan et al., 2006)

inferred from physical interaction with FLYBASE:kon; FB:FBgn0032683

(Estrada et al., 2007)

enables signaling receptor binding

inferred from physical interaction with FLYBASE:fz2; FB:FBgn0016797

(Ataman et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in determination of muscle attachment site

inferred from mutant phenotype

(Swan et al., 2004)

involved_in muscle attachment

inferred from mutant phenotype

(Estrada et al., 2007)

inferred from genetic interaction with FLYBASE:if; FB:FBgn0001250

(Estrada et al., 2007)

inferred from genetic interaction with FLYBASE:mew; FB:FBgn0004456

(Estrada et al., 2007)

inferred from genetic interaction with FLYBASE:kon; FB:FBgn0032683

(Estrada et al., 2007)

involved_in muscle organ morphogenesis

inferred from mutant phenotype

(Swan et al., 2006)

involved_in synapse organization

inferred from mutant phenotype

(Ataman et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in neurotransmitter receptor transport, endosome to postsynaptic membrane

inferred from biological aspect of ancestor with PANTHER:PTN002921324

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in Golgi-associated vesicle

inferred from direct assay

(Ataman et al., 2006)

located_in neuromuscular junction

inferred from direct assay

(Ataman et al., 2006)

located_in terminal bouton

inferred from direct assay

(Ataman et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Glutamate receptor binding protein (Grip) encodes a protein involved in muscle attachment and synapse organization. [Date last reviewed: 2019-09-19]

Summary (Interactive Fly)

Scaffolding and signaling protein - participates in the reception of an attractive signal that emanates from muscle epidermal attachment sites to direct the motility of developing muscles

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Grip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W450)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Grip-RA

FBtr0070865

NM_132057

3764

1058

Grip-RB

FBtr0343413

NM_001297991

3626

1057

Grip-RC

FBtr0343414

NM_001297992

3577

1057

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Grip-PA

FBpp0070830

111.6

1058

6.37

FBpp0310059

111.6

1057

6.37

FBpp0310060

111.6

1057

6.37

X2JIJ6

NP_001284921

AHN59392

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1057 aa isoforms: Grip-PB, Grip-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Grip using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Grip_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.48

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 7 -- 8

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

cardiogenic mesoderm

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

dorsal vessel primordium

Comment: reported as dorsal vessel specific anlage

(Fisher et al., 2012)

fat body/gonad primordium

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in Golgi-associated vesicle

inferred from direct assay

(Ataman et al., 2006)

located_in neuromuscular junction

inferred from direct assay

(Ataman et al., 2006)

located_in terminal bouton

inferred from direct assay

(Ataman et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Grip in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Grip

View exonic expression by tissue for Dmel\Grip

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029830

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50391/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029830

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029830

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029830

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14447

Flygut - An atlas of the Drosophila adult midgut

FBgn0029830

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Grip

Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Grip

Transgenic constructs containing regulatory region of Grip

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 26 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Grip^GD9898

lethal, with Scer\GAL4^pnr-MD237

Grip^GD15768

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Grip^GD9898

viable, with Scer\GAL4^pnr-MD237

Grip^GD9898

viable, with Scer\GAL4^tin.CΔ4

Grip^GD9898

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4^C164

Grip^RNAi.UAS

wild-type, with Scer\GAL4^tey-5053A

Grip^UAS.cSa

Phenotype manifest in

Allele

adult abdomen

adult abdomen (with Grip^ex36)

Grip^ex122

adult abdomen (with Grip^ex122)

Grip^ex36

adult head

adult head (with Grip^ex36)

Grip^ex122

adult head (with Grip^ex122)

Grip^ex36

bouton, with Scer\GAL4^C57

Grip^RNAi.UAS

bouton, with Scer\GAL4^C164

Grip^RNAi.UAS

embryonic somatic muscle cell

Grip^ex36

larval somatic muscle cell

Grip^ex36

lateral transverse muscle cell, with Grip^ex36, Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

lateral transverse muscle cell, with Grip^Δ1-3.UAS, Scer\GAL4^twi.PG

Grip^ex36

lateral transverse muscle cell, with Scer\GAL4^tey-5053A

Grip^UAS.cSa

lateral transverse muscle cell, with Scer\GAL4^twi.PG

lateral transverse muscle cell (with Grip^ex36), with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

lateral transverse muscle cell (with Grip^Δ1-3.UAS), with Scer\GAL4^twi.PG

Grip^ex36

muscle cell of A1-7 ventral longitudinal muscle 1

Grip^ex36

muscle cell of A1-7 ventral longitudinal muscle 1 | precursor

Grip^ex122

muscle cell of abdominal 1 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 1 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 2 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 2 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 3 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 3 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 4 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 4 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 5 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 5 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 6 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 6 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle cell of abdominal 7 dorsal oblique muscle 5, with Scer\GAL4^slou.S59

Grip^Δ1-3.UAS

muscle cell of abdominal 7 dorsal oblique muscle 5, with Scer\GAL4^twi.PG

Grip^Δ1-3.UAS

muscle system

presynaptic active zone, with Scer\GAL4^C164

Grip^RNAi.UAS

ventral longitudinal muscle cell

ventral longitudinal muscle cell, with Grip^ex36, Scer\GAL4^twi.PG

ventral longitudinal muscle cell, with Grip^x3.UAS, Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell, with Grip^x7.UAS, Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell, with Grip^Δ1-3.UAS, Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell, with Grip^Δ6-7.UAS, Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell, with Scer\GAL4^how-24B

Grip^UAS.cSa

ventral longitudinal muscle cell, with Scer\GAL4^twi.PG

ventral longitudinal muscle cell (with Grip^ex36)

Grip^ex122

ventral longitudinal muscle cell (with Grip^ex36), with Scer\GAL4^twi.PG

ventral longitudinal muscle cell (with Grip^ex122)

Grip^ex36

ventral longitudinal muscle cell (with Grip^x3.UAS), with Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell (with Grip^x7.UAS), with Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell (with Grip^Δ1-3.UAS), with Scer\GAL4^twi.PG

Grip^ex36

ventral longitudinal muscle cell (with Grip^Δ6-7.UAS), with Scer\GAL4^twi.PG

Grip^ex36

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (38)

Hsap\GRIP1

9 of 14

Yes

Hsap\GRIP2

9 of 14

Yes

Hsap\DLG3

2 of 14

Hsap\DLG4

2 of 14

Hsap\FRMPD2

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (36)

Rnor\Grip2

10 of 14

Yes

Rnor\Grip1

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tamalin

1 of 14

Rnor\Ush1c

1 of 14

Rnor\Whrn

1 of 14

Mus musculus (laboratory mouse) (37)

Mmus\Grip1

9 of 14

Yes

Mmus\Grip2

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tamalin

1 of 14

Mmus\Ush1c

1 of 14

Mmus\Whrn

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\grip2

8 of 13

Yes

Xtro\grip1

6 of 13

Yes

Xtro\dlg1

2 of 13

Xtro\adcyap1r1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\XB5786944

1 of 13

Danio rerio (Zebrafish) (39)

Drer\grip1

10 of 14

Yes

Drer\grip2a

9 of 14

Yes

Drer\grip2b

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\tamalin

1 of 14

Drer\ush1c

1 of 14

Drer\whrna

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (19)

Cele\C46H11.6

1 of 14

Yes

Cele\C50F7.3

1 of 14

Yes

Cele\C52A11.3

1 of 14

Yes

Cele\dlg-1

1 of 14

Yes

Cele\F16G10.5

1 of 14

Yes

Cele\F20D6.1

1 of 14

Yes

Cele\F23C8.13

1 of 14

Yes

Cele\F28E10.4

1 of 14

Yes

Cele\F40F9.3

1 of 14

Yes

Cele\gras-1

1 of 14

Yes

Cele\magi-1

1 of 14

Yes

Cele\mics-1

1 of 14

Yes

Cele\mpz-4

1 of 14

Yes

Cele\smz-1

1 of 14

Yes

Cele\smz-2

1 of 14

Yes

Cele\T19B10.5

1 of 14

Yes

Cele\txt-1

1 of 14

Yes

Cele\ZK849.1

1 of 14

Yes

Cele\zoo-1

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (28)

Agam\AgaP_AGAP000209

11 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012008

1 of 12

Agam\AgaP_AGAP012354

1 of 12

Agam\AgaP_AGAP013042

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Grip. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (29)

dlg1

2 of 13

scrib

2 of 13

1 of 13

baz

1 of 13

bbg

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pez

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GRIP1; glutamate receptor interacting protein 1

model of Fraser syndrome 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Grip^ex36

exacerbates amyotrophic lateral sclerosis type 2

modeled by Als2^Δ1

(Kim et al., 2021)

Grip^JF02969

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Grip^HMS02178

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GRIP1; glutamate receptor interacting protein 1

9 of 14

604597

FRASER SYNDROME 3; FRASRS3

Fraser syndrome 3

GRIP2; glutamate receptor interacting protein 2

9 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Grip - CG5111

two hybrid

(Hu et al., 2017.6.13)

Grip - ed

two hybrid, filter binding, western blot

(Swan et al., 2006)

Grip - fz2

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Mosca and Schwarz, 2010, Ataman et al., 2006)

Grip - kon

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Estrada et al., 2007, Schnorrer et al., 2007)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Grip

enhanceable

(Swan et al., 2006)

Grip

suppressible

(Swan et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Grip

(Swan et al., 2006)

kon

suppressible

Grip

(Harrison et al., 2021)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72668

DroID - A comprehensive database of gene and protein interactions.

FBgn0029830

MIST (genetic) - An integrated Molecular Interaction Database

50391

MIST (protein-protein) - An integrated Molecular Interaction Database

50391

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Trafficking of GluR2-containing AMPA receptors

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SY14

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-15

Cytogenetic map

5D1-5D1

Sequence location

X:5,966,279..5,986,699 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

5D1-5D1

Limits computationally determined from genome sequence between P{EP}EP1457^EP1457&P{EP}CG5928^EP1083 and P{EP}EP1311&P{EP}EP1384

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-15

(FlyBase, 2016)

1-15.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28334

y¹ v¹; P{TRiP.JF02969}attP2

55472

y¹ w^* Mi{MIC}Grip^MI10366

78864

y¹ w^* Mi{Trojan-GAL4.1}Grip^{MI10366-TG4.1}/FM7c

41978

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02376}attP2

40930

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02178}attP40

Korea

10849

P{EP}Grip^G1820

NIG-Fly

HMS02376

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02376}attP2

HMS02178

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02178}attP40/CyO

VDRC

v103551

P{KK100978}VIE-260B

v21003

w¹¹¹⁸; P{GD9898}v21003/CyO

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (10)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE14068

Other clones

FI03335

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0029830

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0029830

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Deletion studies show that PDZ domains 1 and 2 have a repressive function on the Grip protein.

(Swan et al., 2006)

ed and Grip may form a signaling complex, where competition between the N-terminal and C-terminal PDZ domains of the Grip protein for ed protein binding controls signaling function.

(Swan et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Grip may participate in the reception of an attractive signal that emanates from the epidermal attachment sites to direct the motility of developing muscles.

(Swan et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Annotations CG5980 and CG14447 merged as CG14447 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG14447

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Schnorrer et al., 2007, Boutros et al., 2004, Schulz et al., 2004)

CG5980

(Butler et al., 2003, Sigrist et al., 2003)

DGrip

(Estrada et al., 2007, Swan et al., 2006, Swan et al., 2006, Wichmann et al., 2004)

Dgrip

(Schnorrer et al., 2007)

GRIP

(Das and Cagan, 2017, Nguyen et al., 2016, Liebl and Featherstone, 2008, Kohsaka et al., 2007)

Grip

(Brown et al., 2024, Sun et al., 2024, Du et al., 2021, Zhao et al., 2020, Hu et al., 2017.6.13, Gene Disruption Project members, 2016-, Maartens and Brown, 2015, Ashwal-Fluss et al., 2014, Ibrahim et al., 2013, Aranjuez et al., 2012, Estrada et al., 2007)

dGRIP

(Kim et al., 2021, Peppriell et al., 2021, Koles and Budnik, 2012, Mosca and Schwarz, 2010, Korkut et al., 2009, Ataman, 2006)

dgrip

(Deng et al., 2010, Ataman et al., 2006, Swan et al., 2006, Swan et al., 2004, Sigrist et al., 2003)

grip

(Harrison et al., 2021, Weitkunat and Schnorrer, 2014, Schnorrer and Dickson, 2004)

mab-21

(Brown et al., 2024)

Name Synonyms

Glutamate receptor binding protein

(Sigrist et al., 2003)

Glutamate receptor interacting protein

(Estrada et al., 2007, Swan et al., 2006)

Glutamate-receptor-interacting-protein

(Schnorrer et al., 2007)

Secondary FlyBase IDs

FBgn0040917

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

50391

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W450

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W450

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0029830

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50391/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0029830

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0029830

FlyMine - An integrated database for Drosophila genomics

FBgn0029830

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14447

MARRVEL_MODEL - MARRVEL (model organism gene)

50391

Linkouts

BioGRID - A database of protein and genetic interactions.

72668

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0029830

DroID - A comprehensive database of gene and protein interactions.

FBgn0029830

DRSC - Results frm RNAi screens

FBgn0029830

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Grip_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029830

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0029830

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14447

Flygut - An atlas of the Drosophila adult midgut

FBgn0029830

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0029830

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034397

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

50391

MIST (protein-protein) - An integrated Molecular Interaction Database

50391

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Trafficking of GluR2-containing AMPA receptors

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SY14

References (70)

The following external sites may use different assemblies or annotations than FlyBase.

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