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Gene Dmel\Marf

General Information
Symbol	Dmel\Marf	Species	D. melanogaster
Name	Mitochondrial assembly regulatory factor	Annotation symbol	CG3869
Feature type	protein_coding_gene	FlyBase ID	FBgn0029870
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	dmfn, mfn, mfn2
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	5F4-5F4	Sequence location	X:6,259,982..6,264,960 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mitochondrial assembly regulatory factor is referred to in FlyBase by the symbol Dmel\Marf (CG3869, FBgn0029870). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: GTPase activity. There is experimental evidence that it is involved in the biological process: mitochondrion organization. 9 alleles are reported. The phenotypes of these alleles are annotated with: mitochondrial crista; trichogen cell; adult heart; mitochondrion; adult brain; indirect flight muscle. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Dynamin, GTPase domain; Fzo/mitofusin HR2 domain; Mitofusin family; Transmembrane GTPase Fzo/Fzo1/Marf. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1625464_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Marf. Gene sequence location is X:6259982..6264960.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles Marf[VDRC.cUa] Marf[Scer\UAS.cZa] Transgenic Constructs P{UAS-Marf.Z} P{VDRC.Marf.U} Controlled Vocabulary Terms positive regulation of mitochondrial fusion
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 5F4-5F4   Limits computationally determined from genome sequence between P{EP}EP1402&P{EP}CG15893EP1634 and P{EP}Ctr1AEP1364&P{EP}EP1445
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Marf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Marf-RA FBtr0070908  NM_132092   3677   833 Marf-RB FBtr0070909  NM_206635   3447   810 Marf-RC FBtr0070910  NM_206634   3608   810
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Marf-PA   FBpp0070873   94.1   833   6.76     NP_572320   AAF46162 Marf-PB   FBpp0089222   91.4   810   6.69     NP_996358   AAS65267 Marf-PC   FBpp0089221   91.4   810   6.69     NP_996357   AAS65268
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynamin, GTPase domain (IPR001401) Fzo/mitofusin HR2 domain (IPR006884) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Transmembrane GTPase Fzo/Fzo1/Marf (IPR027091) Mitofusin family (IPR027094)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697028   AA697273   AA697275   AA697664   AA941296   AA941307   AA941694   AA978780   AE014298 AAF46162   AE014298 AAS65267   AE014298 AAS65268   AF355475 AAM00196   AI062197   AI062198   AI063732   AI107043   AI109322   AI292977   AI388980   AI456516   AI512657   AI515919   AI516362   AI517641   AI518062   AI518875   AI532985   AI544394   AW941961   AX106756   AY095019 AAM11347   BF495243   BF503937   BG637714   BI164690   BI166920   BI173246   BI230807   BI235883   BI236979   BI236988   BI237795   BI357162   BI364748   BI366632   BI366745   BI371909   BI373506   BI373537   BI373771   BI373780   BI482364   BI486781   BI604298   BI619137   BI626575   BI627456   BI629924   BT010027 AAQ22496   CO177638   CO185306   CO187254   CO187950   CO188001   CO263219   CO272850   CO278973   CO280812   CO287960   CO296269   CO304584   CO305732   CO308508   CO309113   CO313514   CO314921   CO315192   CO317724   CO330782   EC065710   EC065991   EC068424   EC070749   EC071878   EC079002   EC195216   EC196155   EC201793   EC206615   EC207105   EC210111   EC214348   EC218245   EC228194   EC228796   EC231250   EC232376   EC233877   EC236324   EC237190   EC241071   EC249562   EC249668   EV589033   EV599946   GH941439   GH943289   GH943425   GH944426   GH944935   GH945325   GH945657   GH946346   GH946845   GH947858   GH949433   GH950188   GH950210   GH953131   GH954929   GH955261   GH956632   GH959998   GH960410   GH960471   GH961458   GH961985   GH962097   GH963297   GH963598   GH965644   GH966745   GH968081   GH968114   GH968436   GH969344   GH970968   GH974889   GH977576   GH980714   GH983111   GH984797
UniProtKB/Swiss-Prot	Q7YU24
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Hwa et al., 2002) Comment:maternally contributed embryonic stage 8 -- 10   organism   (Hwa et al., 2002) Comment:zygotically expressed; pattern not described embryonic stage 11   mesoderm   (Hwa et al., 2002) posterior midgut primordium   (Hwa et al., 2002) anterior midgut primordium   (Hwa et al., 2002) embryonic stage 13   embryonic/larval gut   (Hwa et al., 2002) embryonic stage 13 -- 15   embryonic/larval midgut primordium   (Hwa et al., 2002) Comment:strong expression embryonic somatic muscle   (Hwa et al., 2002) Comment:weak expression pharyngeal muscle   (Hwa et al., 2002) Comment:weak expression adult stage | female & oogenesis   nurse cell   (Hwa et al., 2002) Comment:NOT follicle cell oocyte   (Hwa et al., 2002) adult stage | male & spermatogenesis   primary gonial cell   (Hwa et al., 2002) spermatogenesis & adult stage | male   primary spermatocyte   (Hwa et al., 2002) spermatid   (Hwa et al., 2002) northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage | male   organism   (Hwa et al., 2002) adult stage | female   organism   (Hwa et al., 2002) Comment:expressed more strongly in males
Additional Descriptive Data
	Maternally-expressed Marf transcript is present in early embryos, but has degraded before blastoderm stage. Zygotic expression begins at embryonic stage 8. By stage 11,expression is primarily observed in the invaginating midgut and in the mesoderm. During germ band retraction, expression is observed in the developing gut, with strong expression in the midgut. Expression is also observed in somatic muscle and in pharyngeal muscle. In adult females, Marf is expressed in nurse cells and oocytes, but not in follicle cells. In adult males, Marf is expressed in premeiotic spermaticytes, primary spermatocytes, and early-stage spermatids. (Hwa et al., 2002)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval salivary gland, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules    1399 Adult Fat Body    1090.1 Adult Salivary Gland    626.6 Adult Heart    1045.375 Adult VirginFemale Spermatheca    1436 Adult InseminatedFemale Spermatheca    1215.5 Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass    1167 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut  (743.8) Larval Hindgut  (761) Larval Malpighian Tubules  508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea  (672.85) Larval Carcass  (625.675) Adult Head  (656.4) Adult Eye  (896.975) Adult Brain  (782) Adult Thoracic-Abdominal Ganglion  (865.2) Adult Crop  (691.4) Adult Midgut  (595.2) Adult Hindgut  (823.4) Adult Malpighian Tubules  (1399) Adult Fat Body  (1090.1) Adult Salivary Gland  (626.6) Adult Heart  (1045.375) Adult VirginFemale Spermatheca  (1436) Adult InseminatedFemale Spermatheca  (1215.5) Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland  (885.6) Adult Carcass  (1167) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules  (1399) Adult Fat Body  (1090.1) Adult Salivary Gland    626.6 Adult Heart  1045.375 Adult VirginFemale Spermatheca  (1436) Adult InseminatedFemale Spermatheca  (1215.5) Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass  (1167) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules    1399 Adult Fat Body    1090.1 Adult Salivary Gland    626.6 Adult Heart    1045.375 Adult VirginFemale Spermatheca    1436 Adult InseminatedFemale Spermatheca    1215.5 Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass    1167 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules    1399 Adult Fat Body    1090.1 Adult Salivary Gland    626.6 Adult Heart    1045.375 Adult VirginFemale Spermatheca    1436 Adult InseminatedFemale Spermatheca    1215.5 Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass    1167 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut  743.8 Larval Hindgut  761 Larval Malpighian Tubules  508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea  672.85 Larval Carcass  625.675 Adult Head  656.4 Adult Eye  (896.975) Adult Brain  782 Adult Thoracic-Abdominal Ganglion  (865.2) Adult Crop  691.4 Adult Midgut  595.2 Adult Hindgut  (823.4) Adult Malpighian Tubules  (1399) Adult Fat Body  (1090.1) Adult Salivary Gland  626.6 Adult Heart  (1045.375) Adult VirginFemale Spermatheca  (1436) Adult InseminatedFemale Spermatheca  (1215.5) Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland  (885.6) Adult Carcass  (1167) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules  1399 Adult Fat Body  1090.1 Adult Salivary Gland    626.6 Adult Heart  1045.375 Adult VirginFemale Spermatheca  1436 Adult InseminatedFemale Spermatheca  1215.5 Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass  1167 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    385.225 Larval Midgut    743.8 Larval Hindgut    761 Larval Malpighian Tubules    508 Larval Fat Body    251.4 Larval Salivary Gland    358.4 Larval Trachea    672.85 Larval Carcass    625.675 Adult Head    656.4 Adult Eye    896.975 Adult Brain    782 Adult Thoracic-Abdominal Ganglion    865.2 Adult Crop    691.4 Adult Midgut    595.2 Adult Hindgut    823.4 Adult Malpighian Tubules    1399 Adult Fat Body    1090.1 Adult Salivary Gland    626.6 Adult Heart    1045.375 Adult VirginFemale Spermatheca    1436 Adult InseminatedFemale Spermatheca    1215.5 Adult Ovary    466.6 Adult Testis    66.7 Adult Male Accessory Gland    885.6 Adult Carcass    1167 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    656.4   NA  Eye    896.975   NA  Brain    782   385.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    865.2   NA  Crop    691.4   743.8  Midgut    595.2   761  Hindgut    823.4   508  Malpighian Tubules    1399   251.4  Fat Body    1090.1   358.4  Salivary Gland    626.6   NA  Heart    1045.375   672.85  Trachea    NA   NA  VirginFemale Spermatheca    1436   NA  InseminatedFemale Spermatheca    1215.5   NA  Ovary    466.6   NA  Testis    66.7   NA  Male Accessory Gland    885.6   625.675  Carcass    1167 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female  (53) head, virgin 4-day female  (73) head, virgin 20-day female  (69) head, mated 1-day female  (59) head, mated 4-day female  (68) head, mated 20-day female  (59) head, mated 1-day male  (65) head, mated 4-day male  (79) head, mated 20-day male  (100) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult  (43) digestive system, 4-day adult  (51) digestive system, 20-day adult  (54) fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering  (34) carcass, 1-day adult  (71) carcass, 4-day adult  (82) carcass, 20-day adult  (84) ovary, virgin 4-day female  (73) ovary, mated 4-day female  (71) testis, mated 4-day male  (36) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female  (53) head, virgin 4-day female  (73) head, virgin 20-day female  (69) head, mated 1-day female  (59) head, mated 4-day female  (68) head, mated 20-day female  (59) head, mated 1-day male  (65) head, mated 4-day male  (79) head, mated 20-day male  (100) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult  (43) digestive system, 4-day adult  (51) digestive system, 20-day adult  (54) fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering  (34) carcass, 1-day adult  (71) carcass, 4-day adult  (82) carcass, 20-day adult  (84) ovary, virgin 4-day female  (73) ovary, mated 4-day female  (71) testis, mated 4-day male  (36) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    22 central nervous system, pupae P8    24 head, virgin 1-day female    53 head, virgin 4-day female    73 head, virgin 20-day female    69 head, mated 1-day female    59 head, mated 4-day female    68 head, mated 20-day female    59 head, mated 1-day male    65 head, mated 4-day male    79 head, mated 20-day male    100 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    43 digestive system, 4-day adult    51 digestive system, 20-day adult    54 fat body, larvae L3 wandering    16 fat body, white prepupae    13 fat body, pupae P8    15 carcass, larvae L3 wandering    34 carcass, 1-day adult    71 carcass, 4-day adult    82 carcass, 20-day adult    84 ovary, virgin 4-day female    73 ovary, mated 4-day female    71 testis, mated 4-day male    36 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, during late pupal stages, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (83) embryo 02-04hr  (41) embryo 04-06hr  26 embryo 06-08hr  (31) embryo 08-10hr  (36) embryo 10-12hr  (37) embryo 12-14hr  (38) embryo 14-16hr  (47) embryo 16-18hr  (46) embryo 18-20hr  (49) embryo 20-22hr  (55) embryo 22-24hr  (56) larva L1  (51) larva L2  (44) larva L3 12hr old  (44) larva L3 puffstage 1-2  (38) larva L3 puffstage 3-6  (46) larva L3 puffstage 7-9  (37) white prepupae new  (30) white prepupae 12hr    24 white prepupae 24hr  (27) pupae 2d postWPP  (36) pupae 3d postWPP  (59) pupae 4d postWPP  (64) adult male 01day  (87) adult male 05day  (98) adult male 30day  (96) adult female 01day  (90) adult female 05day  (82) adult female 30day  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (83) embryo 02-04hr  (41) embryo 04-06hr  26 embryo 06-08hr  31 embryo 08-10hr  (36) embryo 10-12hr  (37) embryo 12-14hr  (38) embryo 14-16hr  (47) embryo 16-18hr  (46) embryo 18-20hr  (49) embryo 20-22hr  (55) embryo 22-24hr  (56) larva L1  (51) larva L2  (44) larva L3 12hr old  (44) larva L3 puffstage 1-2  (38) larva L3 puffstage 3-6  (46) larva L3 puffstage 7-9  (37) white prepupae new  30 white prepupae 12hr    24 white prepupae 24hr  27 pupae 2d postWPP  (36) pupae 3d postWPP  (59) pupae 4d postWPP  (64) adult male 01day  (87) adult male 05day  (98) adult male 30day  (96) adult female 01day  (90) adult female 05day  (82) adult female 30day  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    41 embryo 04-06hr    26 embryo 06-08hr    31 embryo 08-10hr    36 embryo 10-12hr    37 embryo 12-14hr    38 embryo 14-16hr    47 embryo 16-18hr    46 embryo 18-20hr    49 embryo 20-22hr    55 embryo 22-24hr    56 larva L1    51 larva L2    44 larva L3 12hr old    44 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    37 white prepupae new    30 white prepupae 12hr    24 white prepupae 24hr    27 pupae 2d postWPP    36 pupae 3d postWPP    59 pupae 4d postWPP    64 adult male 01day    87 adult male 05day    98 adult male 30day    96 adult female 01day    90 adult female 05day    82 adult female 30day    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  (31) embryonic 1182-4H  (47) embryonic GM2  (80) embryonic Kc167  (58) embryonic S1  (61) embryonic S3  (38) leg disc CME L1  (37) wing disc CME-W2  (33) wing disc ML-DmD8  (31) wing disc ML-DmD9  (58) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (34) wing disc ML-DmD32  (52) haltere disc ML-DmD17-c3  (45) eye-antennal disc ML-DmD11  (50) antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1  (35) CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2  (28) ovary fGS/OSS  (51) ovary OSC  (40) ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  31 embryonic 1182-4H  (47) embryonic GM2  (80) embryonic Kc167  (58) embryonic S1  (61) embryonic S3  (38) leg disc CME L1  (37) wing disc CME-W2  (33) wing disc ML-DmD8  31 wing disc ML-DmD9  (58) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  (34) wing disc ML-DmD32  (52) haltere disc ML-DmD17-c3  (45) eye-antennal disc ML-DmD11  (50) antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1  (35) CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2  28 ovary fGS/OSS  (51) ovary OSC  (40) ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    31 embryonic 1182-4H    47 embryonic GM2    80 embryonic Kc167    58 embryonic S1    61 embryonic S3    38 leg disc CME L1    37 wing disc CME-W2    33 wing disc ML-DmD8    31 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    34 wing disc ML-DmD32    52 haltere disc ML-DmD17-c3    45 eye-antennal disc ML-DmD11    50 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    35 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    28 ovary fGS/OSS    51 ovary OSC    40 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (39) cold shock, 4-day adult    25 heat shock, 4-day adult  (47) Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult  (52) Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult  (76) Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult  (76) Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (42) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (81) Caffeine 25 mg/ml 48 hrs, 4-day adult  (38) Paraquat 5 mM 48 hrs, 4-day adult  (82) Paraquat 10 mM 48 hrs, 4-day adult  (100) Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (39) cold shock, 4-day adult    25 heat shock, 4-day adult  (47) Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult  (52) Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult  (76) Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult  (76) Ethanol 2.5% 3 hrs, larvae L3  (73) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (42) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (81) Caffeine 25 mg/ml 48 hrs, 4-day adult  (38) Paraquat 5 mM 48 hrs, 4-day adult  (82) Paraquat 10 mM 48 hrs, 4-day adult  (100) Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    25 heat shock, 4-day adult    47 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    52 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    24 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    15 Zinc 4.5 mM 48 hrs, 4-day adult    76 Ethanol 2.5% 3 hrs, larvae L3    73 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    25 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    81 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    82 Paraquat 10 mM 48 hrs, 4-day adult    100 Rotenone 2 μg 12 hrs, larvae L3    21 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_26 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029870 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3869-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage, with Scer\GAL4tub.PU MarfmiRNA.cUa.Scer\UAS viable, with Scer\GAL4pnr-MD237 MarfGD11094 viable, with Scer\GAL4tin.CΔ4 MarfmiRNA.cUa.Scer\UAS Other Phenotypes Allele cell death defective, with Scer\GAL4elav.PU MarfScer\UAS.cDa gravitaxis defective | progressive, with Scer\GAL4tin.CΔ4 MarfmiRNA.cUa.Scer\UAS increased cell number | third instar larval stage, with Scer\GAL4Antp-10 MarfGD11094 MarfKK105681 neuroanatomy defective, with Scer\GAL4elav.PU MarfScer\UAS.cDa visible, with Scer\GAL4ey.3.5.Exel MarfmiRNA.cUa.Scer\UAS visible, with Scer\GAL4pnr-MD237 MarfGD11094 Phenotype manifest in Allele adult brain, with Scer\GAL4elav.PU MarfScer\UAS.cDa adult heart, with Scer\GAL4tin.CΔ4 MarfmiRNA.cUa.Scer\UAS cardial cell | adult stage, with Scer\GAL4tin.CΔ4 MarfmiRNA.cUa.Scer\UAS eye, with Scer\GAL4ey.3.5.Exel MarfmiRNA.cUa.Scer\UAS indirect flight muscle, with Scer\GAL4Mef2.PR MarfScer\UAS.T:Zzzz\FLAG lamellocyte | third instar larval stage, with Scer\GAL4Antp-10 MarfGD11094 MarfKK105681 mitochondrial crista, with Scer\GAL4Mef2.PR MarfmiRNA.CDS.Scer\UAS MarfmiRNA.UTR.Scer\UAS mitochondrion, with Scer\GAL4elav.PU MarfScer\UAS.cDa mitochondrion, with Scer\GAL4Mef2.PR MarfScer\UAS.T:Zzzz\FLAG MarfmiRNA.CDS.Scer\UAS MarfmiRNA.UTR.Scer\UAS mitochondrion, with Scer\GAL4Mhc.PW MarfGD11094 MarfKK105681 mitochondrion & dopaminergic neuron, with Scer\GAL4ple.PF MarfScer\UAS.T:Zzzz\FLAG mitochondrion | adult stage, with Scer\GAL4tin.CΔ4 MarfmiRNA.cUa.Scer\UAS ommatidium, with Scer\GAL4ey.3.5.Exel MarfmiRNA.cUa.Scer\UAS trichogen cell, with Scer\GAL4pnr-MD237 MarfGD11094
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Marf Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Marf Class Mutagen Stocks Known lesion MarfGD11094 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MarfJF01650 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MarfKK105681 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MarfmiRNA.CDS.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes MarfmiRNA.cUa.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes MarfmiRNA.UTR.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes MarfNIG.3869R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MarfScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes MarfScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes MarfScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MarfVDRC.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 --
Aneuploid Aberrations
Disrupted in	Df(1)Exel6239 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11094} NA P{KK105681} NA P{NIG.3869R} NA P{TRiP.JF01650} NA P{UAS-Marf.D} NA P{UAS-Marf.Flag} NA P{UAS-Marf.miRNA.CDS} NA P{UAS-Marf.miRNA.U} NA P{UAS-Marf.miRNA.UTR} NA P{UAS-Marf.Z} NA characterization construct P{VDRC.Marf.U} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitochondrion organization inferred from mutant phenotype (Eschenbacher et al., 2012, Deng et al., 2008, Dorn et al., 2011) positive regulation of mitochondrial fusion inferred from mutant phenotype (Ziviani et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from sequence or structural similarity with UniProtKB:O18412 (assigned by UniProtKB) (Hwa et al., 2002) GTP binding inferred from electronic annotation with InterPro:IPR001401 (FlyBase Curators et al., 2004-) non-traceable author statement (Bach, 2001.3.2)
Biological Process
CV term Evidence References
GTP catabolic process inferred from electronic annotation with InterPro:IPR006884 (FlyBase Curators et al., 2004-) mitochondrial fusion inferred from sequence or structural similarity with UniProtKB:O18412 (assigned by UniProtKB) (Hwa et al., 2002) mitochondrion organization non-traceable author statement (Bach, 2001.3.2)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR006884 (FlyBase Curators et al., 2004-) mitochondrial envelope inferred from sequence or structural similarity with UniProtKB:O18412 (assigned by UniProtKB) (Hwa et al., 2002) mitochondrial outer membrane inferred from electronic annotation with InterPro:IPR006884 (FlyBase Curators et al., 2004-) plasma membrane non-traceable author statement (Bach, 2001.3.2)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Marf allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58068 DroID - A comprehensive database of gene and protein interactions. • FBgn0029870 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31581
Orthologs
OrthoDB Orthologs (49) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6F1XNH Dipteran inclusive ortholog search EOG6ZPF3C Insect inclusive ortholog search EOG676HV7 Arthropod inclusive ortholog search EOG6DZ096 Metazoa inclusive ortholog search EOG6NKQNG
Orthologs in Drosophila Species (EOG6F1XNH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Marf     Drosophila simulans   Dsim\GD16797 Y   Drosophila sechellia   Dsec\GM12488 Y   Drosophila erecta   Dere\GG19591 Y   Drosophila yakuba   Dyak\GE16751 Y   Drosophila ananassae   Dana\GF19353 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17739 Y   Drosophila persimilis   Dper\GL26855 Y   Drosophila willistoni   Dwil\GK16527 Y   Drosophila virilis   Dvir\GJ17071 Y   Drosophila mojavensis   Dmoj\Marf Y   Drosophila grimshawi   Dgri\GH24062 Y
Orthologs in non-Drosophila Dipterans (EOG6ZPF3C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004471   Anopheles gambiae Malaria mosquito  Agam\AGAP001802   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ018960   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013828
Orthologs in non-Dipteran Insects (EOG676HV7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45207   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012130   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH27023   Atta cephalotes Leafcutter ant  Acep\ACEP20387   Atta cephalotes Leafcutter ant  Acep\ACEP20388   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17303   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13561   Linepithema humile Argentine ant  Lhum\LH16796   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18012   Solenopsis invicta Red fire ant  Sinv\SINV26108   Solenopsis invicta Red fire ant  Sinv\SINV21156   Acyrthosiphon pisum Pea aphid  Apim\ACYPI069627   Bombyx mori Silkmoth  Bmor\BGIBMGA002820   Pediculus humanus Human body louse  Phum\PHUM579670   Tribolium castaneum Red flour beetle  Tcas\TC011638
Orthologs in non-Insect Arthropods (EOG6DZ096)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Bpnt1   Ixodes scapularis Deer tick  Isca\ISCW010714
Orthologs in non-Arthropod Metazoa (EOG6NKQNG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\fzo-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007142gn Y Danio rerio Zebrafish  Drer\mfn1 Y Danio rerio Zebrafish  Drer\ENSDARG00000079219 Y Danio rerio Zebrafish  Drer\DKEYP-104H9.2 Y Danio rerio Zebrafish  Drer\ENSDARG00000011884 Y Xenopus tropicalis Western clawed frog  Xtro\mfn2 Y Xenopus tropicalis Western clawed frog  Xtro\mfn1 Y Gallus gallus Domestic chicken  Ggal\MFN1 Y Gallus gallus Domestic chicken  Ggal\MFN2 Y Mus musculus House mouse  Mmus\Mfn2 Y Mus musculus House mouse  Mmus\Mfn1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000011057 Y Rattus norvegicus Norway rat  Rnor\Mfn2 Y Homo sapiens Human  Hsap\MFN2 Y Homo sapiens Human  Hsap\MFN1 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\MFN2     Hsap\MFN1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16797 Drosophila sechellia Dsec\GM12488 Drosophila erecta Dere\GG19591 Drosophila yakuba Dyak\GE16751 Drosophila ananassae Dana\GF19353 Drosophila pseudoobscura pseudoobscura Dpse\GA17739 Drosophila persimilis Dper\GL26855 Drosophila willistoni Dwil\GK16527 Drosophila virilis Dvir\GJ17071 Drosophila mojavensis Dmoj\Marf Drosophila grimshawi Dgri\GH24062
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	31157 y1 v1; P{TRiP.JF01650}attP2
VDRC	v105261 P{KK105681}VIE-260B v40478 w1118; P{GD11094}v40478
Genomic Clones ( 2 )
	BACR18C18 BACR19D03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 133 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM08958 RE04414
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04121 AT05176 GH01322 GH01323 GH03702 GH06331 GH08471 GH16027 GH20046 GH28679 HL02120 HL02122 HL02951 LD25359 LD25371 LD27055 LD32937 LD35291 LD36560 LD37472 LD41626 LD42213 LD44583 RE07166 RE15251 RE16029 RE31834 RE33120 RE33129 RE34093 RE43557 RE49924 RE52214 RE52346 RE58935 RE60815 RE60853 RE61178 RE61189 RE65048 RE70245 RH50114 RH58865 RH67557 RH68782 RH69611 SD04650 SD10937
Other clones	8994 9378 19224 41687 81600 82184 107119 119668 122158 146745 153970 157351 193262 275456 CK01.3A.A8 CK01.3C.G12 CK01.39D.A10 CK01.41A.E11 CK01.41D.E11 CK01.57B.E8 CK01.66B.E10 CK01.85A.F5 CK01.105A.C11 Dmel_neb_005529_0324_2722 Dmel_neb_007236_0822_3088 EK007858 EK027514 EK035215 EK035715 EK053949 EK067423 EK087812 EK108542 EK132534 EK154929 EK213258 EK233149 EK238402 EK239633 EK245219 EK249324 EK258545 EK260334 EK268901 EK296154 LD01096 RP004074324 RP004074733 RP004100912 RP004106610 RP004134318 RP004175452 RP004175931 RP004185259 RP004204362 RP004214027 RP004216966 RP004222821 RP004228455 RP004232142 RP004234354 RP004238571 RP004240697 RP004251445 RP004252251 RP004267075 RP004286805 RP004355203 RP004419349 RP004443053 RP004455056 RP004459589 RP004459791 RP004475438 RP004488657 RP004512438 RP004516003 RP004528946 RP004529552 RP004535307 RP004545284 RP004545663 RP004549970
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0029870 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31581
Antibody Information
	polyclonal (Poole et al., 2010, Ziviani et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Marf CG3869 (FlyBase, 1996-)
Source for database merge of	Source for merge of: Marf anon-WO0125274.3 (FlyBase, 1996-)
Additional comments	Source for merge of Marf anon-WO0125274.3 was sequence comparison (date:051113). (FlyBase, 1996-) Source for identity of Marf CG3869 was sequence comparison (date:020416). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132092 NM_206634 NM_206635 RefSeq (Proteins) • NP_572320 NP_996357 NP_996358 DDBJ / EMBL / GenBank • AA697028 AAF46162 AAF46162.1 AAM00196 AAM11347 AAQ22496 AAS65267 AAS65267.1 AAS65268 AAS65268.1 AF355475 AW941961 AX106756 AY095019 BI164690 BT010027 Entrez Gene - A searchable database of RefSeq genes. • 31581 UniProtKB/Swiss-Prot • Q7YU24
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynamin, GTPase domain (IPR001401) Fzo/mitofusin HR2 domain (IPR006884) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Transmembrane GTPase Fzo/Fzo1/Marf (IPR027091) Mitofusin family (IPR027094)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58068 DroID - A comprehensive database of gene and protein interactions. • FBgn0029870 DRSC - Results from RNAi screens. • FBgn0029870 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0029870 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3869-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0029870 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31581 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31581 modMine - Data generated by the modENCODE project. • FBgn0029870
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	anon-WO0125274.3   CG3869 (Bonacum et al., 2001, Poole et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2008) dmfn (Hwa et al., 2002) dMfn (Eschenbacher et al., 2012, Poole et al., 2010) dMFN (Thomenius et al., 2011) Marf (Schmidt et al., 2012, Sinenko et al., 2011, Bakal et al., 2007, Thomenius et al., 2011, Juhász et al., 2007, Deng et al., 2008, Ziviani et al., 2010, Boyd et al., 2011, Dorn et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Sinenko et al., 2011) MARF (Fergestad et al., 2006, Eschenbacher et al., 2012, Duboff et al., 2012) marf (Rival et al., 2011, Park et al., 2009, Park et al., 2010) Marf-1 (Mitra et al., 2012) mfn (Rival et al., 2011, Imai et al., 2010) Mfn (Ziviani et al., 2010) mfn2 (Rival et al., 2011, Poole et al., 2008)
Name Synonym	Drosophila mitofusin (Hwa et al., 2002) Mitochondrial assembly regulatory factor (Sinenko et al., 2011) mitochondrial assembly regulatory factor (Boyd et al., 2011, Dorn et al., 2011) mitofusin (Rival et al., 2011) Mitofusin (Poole et al., 2010, Ziviani et al., 2010)
Secondary FlyBase IDs
	FBgn0044368
References ( 41 )
Generate a list of	All references relating to this gene ( 41 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 ) Patent  ( 1 )
Recent research papers ( 11 )
	Burman et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(26): 10438--10443 Analysis of neural subtypes reveals selective mitochondrial dysfunction in dopaminergic neurons from parkin mutants. [FBrf0218718] Duboff et al., 2012, Neuron 75(4): 618--632 Tau Promotes Neurodegeneration via DRP1 Mislocalization In Vivo. [FBrf0219271] Eschenbacher et al., 2012, PLoS ONE 7(9): e44296 Two rare human mitofusin 2 mutations alter mitochondrial dynamics and induce retinal and cardiac pathology in Drosophila. [FBrf0219392] Mitra et al., 2012, J. Cell Biol. 197(4): 487--497 DRP1-dependent mitochondrial fission initiates follicle cell differentiation during Drosophila oogenesis. [FBrf0218275] Oliveira et al., 2012, Molec. Phylog. Evol. 64(3): 533--544 Monophyly, divergence times, and evolution of host plant use inferred from a revised phylogeny of the Drosophila repleta species group. [FBrf0218731] Schmidt et al., 2012, J. Neurosci. 32(22): 7466--7476 Kinesin heavy chain function in Drosophila glial cells controls neuronal activity. [FBrf0218491] Boyd et al., 2011, Free Radical Biol. Med. 50(11): 1669--1678 Nectarine promotes longevity in Drosophila melanogaster. [FBrf0213612] Dorn et al., 2011, Circulation Res. 108(1): 12--17 MARF and Opa1 Control Mitochondrial and Cardiac Function in Drosophila. [FBrf0212708] Rival et al., 2011, EMBO Rep. 12(3): 223--230 Inner-membrane proteins PMI/TMEM11 regulate mitochondrial morphogenesis independently of the DRP1/MFN fission/fusion pathways. [FBrf0213174] Sinenko et al., 2011, EMBO Rep. 13(1): 83--89 Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. [FBrf0217071] Thomenius et al., 2011, Cell Death Differ. 18(10): 1640--1650 Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death. [FBrf0215252] Show all research papers ...