Gene Dmel\CG18624

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General Information

Symbol

Dmel\CG18624

Species

D. melanogaster

Name

Annotation symbol

CG18624

Feature type

protein_coding_gene

FlyBase ID

FBgn0029971

Gene Model Status

Current

Stock availability

4 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

7C2-7C2

Sequence location

X:7,787,086..7,787,831 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG18624 (FBgn0029971). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: NADH dehydrogenase activity. Based on sequence similarity, it is predicted to be involved in the biological process: mitochondrial electron transport, NADH to ubiquinone. 2 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: NADH:ubiquinone oxidoreductase, MNLL subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval fat body, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1638721_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of CG18624. Gene sequence location is X:7787086..7787831.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

7C2-7C2

Limits computationally determined from genome sequence between P{EP}EP1209 and P{EP}CG10777^EP1347&P{EP}CG10777^EP1363

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG18624 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

FBtr0071140

487

FBtr0071141

509

FBtr0300899

413

FBtr0300900

649

FBtr0340163

575

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG18624-PA

FBpp0071092

6.2

10.36

NP_572416

AAF46285

CG18624-PB

FBpp0071093

6.2

10.36

NP_727209

AAN09217

CG18624-PC

FBpp0290121

6.2

10.36

NP_001162693

ACZ95229

CG18624-PD

FBpp0290122

6.2

10.36

NP_001162694

ACZ95230

CG18624-PE

FBpp0309149

6.2

10.36

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

NADH:ubiquinone oxidoreductase, MNLL subunit (IPR012575)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME18624

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W3N7

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval fat body, adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		1524.9
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		1564.775
Adult Head		1161.6
Adult Eye		1949.85
Adult Brain		1243.2
Adult Thoracic-Abdominal Ganglion		1619.7
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		1642
Adult Malpighian Tubules		1226.9
Adult Fat Body		1069.3
Adult Salivary Gland		2033.4
Adult Heart		1426
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		1827.6
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(673.925)
Larval Midgut		(933.2)
Larval Hindgut		(1524.9)
Larval Malpighian Tubules		(611)
Larval Fat Body		333.5
Larval Salivary Gland		(931.3)
Larval Trachea		(1015.875)
Larval Carcass		(1564.775)
Adult Head		(1161.6)
Adult Eye		(1949.85)
Adult Brain		(1243.2)
Adult Thoracic-Abdominal Ganglion		(1619.7)
Adult Crop		(1021.8)
Adult Midgut		(994.5)
Adult Hindgut		(1642)
Adult Malpighian Tubules		(1226.9)
Adult Fat Body		(1069.3)
Adult Salivary Gland		(2033.4)
Adult Heart		(1426)
Adult VirginFemale Spermatheca		(982.3)
Adult InseminatedFemale Spermatheca		(943)
Adult Ovary		(624.4)
Adult Testis		310.1
Adult Male Accessory Gland		(781.9)
Adult Carcass		(1827.6)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		(1524.9)
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		(1564.775)
Adult Head		(1161.6)
Adult Eye		(1949.85)
Adult Brain		(1243.2)
Adult Thoracic-Abdominal Ganglion		(1619.7)
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		(1642)
Adult Malpighian Tubules		(1226.9)
Adult Fat Body		1069.3
Adult Salivary Gland		(2033.4)
Adult Heart		(1426)
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		(1827.6)
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		1524.9
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		1564.775
Adult Head		1161.6
Adult Eye		1949.85
Adult Brain		1243.2
Adult Thoracic-Abdominal Ganglion		1619.7
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		1642
Adult Malpighian Tubules		1226.9
Adult Fat Body		1069.3
Adult Salivary Gland		2033.4
Adult Heart		1426
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		1827.6
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		1524.9
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		1564.775
Adult Head		1161.6
Adult Eye		1949.85
Adult Brain		1243.2
Adult Thoracic-Abdominal Ganglion		1619.7
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		1642
Adult Malpighian Tubules		1226.9
Adult Fat Body		1069.3
Adult Salivary Gland		2033.4
Adult Heart		1426
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		1827.6
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		(933.2)
Larval Hindgut		(1524.9)
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		(931.3)
Larval Trachea		(1015.875)
Larval Carcass		(1564.775)
Adult Head		(1161.6)
Adult Eye		(1949.85)
Adult Brain		(1243.2)
Adult Thoracic-Abdominal Ganglion		(1619.7)
Adult Crop		(1021.8)
Adult Midgut		(994.5)
Adult Hindgut		(1642)
Adult Malpighian Tubules		(1226.9)
Adult Fat Body		(1069.3)
Adult Salivary Gland		(2033.4)
Adult Heart		(1426)
Adult VirginFemale Spermatheca		(982.3)
Adult InseminatedFemale Spermatheca		(943)
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		(1827.6)
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		1524.9
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		1564.775
Adult Head		1161.6
Adult Eye		(1949.85)
Adult Brain		1243.2
Adult Thoracic-Abdominal Ganglion		1619.7
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		1642
Adult Malpighian Tubules		1226.9
Adult Fat Body		1069.3
Adult Salivary Gland		(2033.4)
Adult Heart		1426
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		(1827.6)
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		673.925
Larval Midgut		933.2
Larval Hindgut		1524.9
Larval Malpighian Tubules		611
Larval Fat Body		333.5
Larval Salivary Gland		931.3
Larval Trachea		1015.875
Larval Carcass		1564.775
Adult Head		1161.6
Adult Eye		1949.85
Adult Brain		1243.2
Adult Thoracic-Abdominal Ganglion		1619.7
Adult Crop		1021.8
Adult Midgut		994.5
Adult Hindgut		1642
Adult Malpighian Tubules		1226.9
Adult Fat Body		1069.3
Adult Salivary Gland		2033.4
Adult Heart		1426
Adult VirginFemale Spermatheca		982.3
Adult InseminatedFemale Spermatheca		943
Adult Ovary		624.4
Adult Testis		310.1
Adult Male Accessory Gland		781.9
Adult Carcass		1827.6
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	1161.6
NA	Eye	1949.85
NA	Brain	1243.2
673.925	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1619.7
NA	Crop	1021.8
933.2	Midgut	994.5
1524.9	Hindgut	1642
611	Malpighian Tubules	1226.9
333.5	Fat Body	1069.3
931.3	Salivary Gland	2033.4
NA	Heart	1426
1015.875	Trachea	NA
NA	VirginFemale Spermatheca	982.3
NA	InseminatedFemale Spermatheca	943
NA	Ovary	624.4
NA	Testis	310.1
NA	Male Accessory Gland	781.9
1564.775	Carcass	1827.6

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		165
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		169
head, mated 4-day male		82
head, mated 20-day male		124
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		117
carcass, larvae L3 wandering		40
carcass, 1-day adult		168
carcass, 4-day adult		104
carcass, 20-day adult		117
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(62)
central nervous system, larvae L3		(34)
central nervous system, pupae P8		(35)
head, virgin 1-day female		(165)
head, virgin 4-day female		(107)
head, virgin 20-day female		(105)
head, mated 1-day female		(102)
head, mated 4-day female		(61)
head, mated 20-day female		(75)
head, mated 1-day male		(169)
head, mated 4-day male		(82)
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		(48)
digestive system, 1-day adult		(103)
digestive system, 4-day adult		(64)
digestive system, 20-day adult		(76)
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		(117)
carcass, larvae L3 wandering		(40)
carcass, 1-day adult		(168)
carcass, 4-day adult		(104)
carcass, 20-day adult		(117)
ovary, virgin 4-day female		(93)
ovary, mated 4-day female		(76)
testis, mated 4-day male		(61)
accessory gland, mated 4-day male		(58)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		(165)
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		(169)
head, mated 4-day male		82
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		(117)
carcass, larvae L3 wandering		40
carcass, 1-day adult		(168)
carcass, 4-day adult		104
carcass, 20-day adult		(117)
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		165
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		169
head, mated 4-day male		82
head, mated 20-day male		124
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		117
carcass, larvae L3 wandering		40
carcass, 1-day adult		168
carcass, 4-day adult		104
carcass, 20-day adult		117
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		165
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		169
head, mated 4-day male		82
head, mated 20-day male		124
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		117
carcass, larvae L3 wandering		40
carcass, 1-day adult		168
carcass, 4-day adult		104
carcass, 20-day adult		117
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(62)
central nervous system, larvae L3		(34)
central nervous system, pupae P8		(35)
head, virgin 1-day female		(165)
head, virgin 4-day female		(107)
head, virgin 20-day female		(105)
head, mated 1-day female		(102)
head, mated 4-day female		(61)
head, mated 20-day female		(75)
head, mated 1-day male		(169)
head, mated 4-day male		(82)
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		(48)
digestive system, 1-day adult		(103)
digestive system, 4-day adult		(64)
digestive system, 20-day adult		(76)
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		(117)
carcass, larvae L3 wandering		(40)
carcass, 1-day adult		(168)
carcass, 4-day adult		(104)
carcass, 20-day adult		(117)
ovary, virgin 4-day female		(93)
ovary, mated 4-day female		(76)
testis, mated 4-day male		(61)
accessory gland, mated 4-day male		(58)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		(165)
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		(169)
head, mated 4-day male		82
head, mated 20-day male		124
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		117
carcass, larvae L3 wandering		40
carcass, 1-day adult		(168)
carcass, 4-day adult		104
carcass, 20-day adult		117
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		34
central nervous system, pupae P8		35
head, virgin 1-day female		165
head, virgin 4-day female		107
head, virgin 20-day female		105
head, mated 1-day female		102
head, mated 4-day female		61
head, mated 20-day female		75
head, mated 1-day male		169
head, mated 4-day male		82
head, mated 20-day male		124
salivary gland, larvae L3 wandering		15
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		48
digestive system, 1-day adult		103
digestive system, 4-day adult		64
digestive system, 20-day adult		76
fat body, larvae L3 wandering		10
fat body, white prepupae		18
fat body, pupae P8		117
carcass, larvae L3 wandering		40
carcass, 1-day adult		168
carcass, 4-day adult		104
carcass, 20-day adult		117
ovary, virgin 4-day female		93
ovary, mated 4-day female		76
testis, mated 4-day male		61
accessory gland, mated 4-day male		58
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		120
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		134
embryo 16-18hr		155
embryo 18-20hr		158
embryo 20-22hr		170
embryo 22-24hr		167
larva L1		253
larva L2		211
larva L3 12hr old		205
larva L3 puffstage 1-2		132
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		108
pupae 3d postWPP		248
pupae 4d postWPP		338
adult male 01day		284
adult male 05day		232
adult male 30day		185
adult female 01day		293
adult female 05day		144
adult female 30day		136
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(120)
embryo 02-04hr		(95)
embryo 04-06hr		(83)
embryo 06-08hr		(81)
embryo 08-10hr		(90)
embryo 10-12hr		(98)
embryo 12-14hr		(84)
embryo 14-16hr		(134)
embryo 16-18hr		(155)
embryo 18-20hr		(158)
embryo 20-22hr		(170)
embryo 22-24hr		(167)
larva L1		(253)
larva L2		(211)
larva L3 12hr old		(205)
larva L3 puffstage 1-2		(132)
larva L3 puffstage 3-6		(72)
larva L3 puffstage 7-9		(62)
white prepupae new		(48)
white prepupae 12hr		(48)
white prepupae 24hr		(49)
pupae 2d postWPP		(108)
pupae 3d postWPP		(248)
pupae 4d postWPP		(338)
adult male 01day		(284)
adult male 05day		(232)
adult male 30day		(185)
adult female 01day		(293)
adult female 05day		(144)
adult female 30day		(136)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(120)
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		(134)
embryo 16-18hr		(155)
embryo 18-20hr		(158)
embryo 20-22hr		(170)
embryo 22-24hr		(167)
larva L1		(253)
larva L2		(211)
larva L3 12hr old		(205)
larva L3 puffstage 1-2		(132)
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		(108)
pupae 3d postWPP		(248)
pupae 4d postWPP		(338)
adult male 01day		(284)
adult male 05day		(232)
adult male 30day		(185)
adult female 01day		(293)
adult female 05day		(144)
adult female 30day		(136)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		120
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		134
embryo 16-18hr		155
embryo 18-20hr		158
embryo 20-22hr		170
embryo 22-24hr		167
larva L1		253
larva L2		211
larva L3 12hr old		205
larva L3 puffstage 1-2		132
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		108
pupae 3d postWPP		248
pupae 4d postWPP		338
adult male 01day		284
adult male 05day		232
adult male 30day		185
adult female 01day		293
adult female 05day		144
adult female 30day		136
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		120
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		134
embryo 16-18hr		155
embryo 18-20hr		158
embryo 20-22hr		170
embryo 22-24hr		167
larva L1		253
larva L2		211
larva L3 12hr old		205
larva L3 puffstage 1-2		132
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		108
pupae 3d postWPP		248
pupae 4d postWPP		338
adult male 01day		284
adult male 05day		232
adult male 30day		185
adult female 01day		293
adult female 05day		144
adult female 30day		136
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(120)
embryo 02-04hr		(95)
embryo 04-06hr		(83)
embryo 06-08hr		(81)
embryo 08-10hr		(90)
embryo 10-12hr		(98)
embryo 12-14hr		(84)
embryo 14-16hr		(134)
embryo 16-18hr		(155)
embryo 18-20hr		(158)
embryo 20-22hr		(170)
embryo 22-24hr		(167)
larva L1		(253)
larva L2		(211)
larva L3 12hr old		(205)
larva L3 puffstage 1-2		(132)
larva L3 puffstage 3-6		(72)
larva L3 puffstage 7-9		(62)
white prepupae new		(48)
white prepupae 12hr		(48)
white prepupae 24hr		(49)
pupae 2d postWPP		(108)
pupae 3d postWPP		(248)
pupae 4d postWPP		(338)
adult male 01day		(284)
adult male 05day		(232)
adult male 30day		(185)
adult female 01day		(293)
adult female 05day		(144)
adult female 30day		(136)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		120
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		134
embryo 16-18hr		(155)
embryo 18-20hr		(158)
embryo 20-22hr		(170)
embryo 22-24hr		(167)
larva L1		(253)
larva L2		(211)
larva L3 12hr old		(205)
larva L3 puffstage 1-2		132
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		108
pupae 3d postWPP		(248)
pupae 4d postWPP		(338)
adult male 01day		(284)
adult male 05day		(232)
adult male 30day		(185)
adult female 01day		(293)
adult female 05day		(144)
adult female 30day		136
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		120
embryo 02-04hr		95
embryo 04-06hr		83
embryo 06-08hr		81
embryo 08-10hr		90
embryo 10-12hr		98
embryo 12-14hr		84
embryo 14-16hr		134
embryo 16-18hr		155
embryo 18-20hr		158
embryo 20-22hr		170
embryo 22-24hr		167
larva L1		253
larva L2		211
larva L3 12hr old		205
larva L3 puffstage 1-2		132
larva L3 puffstage 3-6		72
larva L3 puffstage 7-9		62
white prepupae new		48
white prepupae 12hr		48
white prepupae 24hr		49
pupae 2d postWPP		108
pupae 3d postWPP		248
pupae 4d postWPP		338
adult male 01day		284
adult male 05day		232
adult male 30day		185
adult female 01day		293
adult female 05day		144
adult female 30day		136
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		129
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(85)
Schneider line 2 Sg4		(38)
embryonic 1182-4H		(67)
embryonic GM2		(98)
embryonic Kc167		(32)
embryonic S1		(97)
embryonic S3		(94)
leg disc CME L1		(28)
wing disc CME-W2		(43)
wing disc ML-DmD8		(63)
wing disc ML-DmD9		(81)
wing disc ML-DmD16-c3		(40)
wing disc ML-DmD21		(68)
wing disc ML-DmD32		(47)
haltere disc ML-DmD17-c3		(98)
eye-antennal disc ML-DmD11		(129)
antennal disc ML-DmD20-c5		(77)
mixed discs ML-DmD4-c1		(34)
CNS ML-DmBG1-c1		(49)
CNS ML-DmBG2-c2		(84)
tumorous blood cells mbn2		(46)
ovary fGS/OSS		(68)
ovary OSC		(63)
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		(129)
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		129
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		129
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(85)
Schneider line 2 Sg4		(38)
embryonic 1182-4H		(67)
embryonic GM2		(98)
embryonic Kc167		32
embryonic S1		(97)
embryonic S3		(94)
leg disc CME L1		28
wing disc CME-W2		(43)
wing disc ML-DmD8		(63)
wing disc ML-DmD9		(81)
wing disc ML-DmD16-c3		(40)
wing disc ML-DmD21		(68)
wing disc ML-DmD32		(47)
haltere disc ML-DmD17-c3		(98)
eye-antennal disc ML-DmD11		(129)
antennal disc ML-DmD20-c5		(77)
mixed discs ML-DmD4-c1		(34)
CNS ML-DmBG1-c1		(49)
CNS ML-DmBG2-c2		(84)
tumorous blood cells mbn2		(46)
ovary fGS/OSS		(68)
ovary OSC		(63)
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		129
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		85
Schneider line 2 Sg4		38
embryonic 1182-4H		67
embryonic GM2		98
embryonic Kc167		32
embryonic S1		97
embryonic S3		94
leg disc CME L1		28
wing disc CME-W2		43
wing disc ML-DmD8		63
wing disc ML-DmD9		81
wing disc ML-DmD16-c3		40
wing disc ML-DmD21		68
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		98
eye-antennal disc ML-DmD11		129
antennal disc ML-DmD20-c5		77
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		84
tumorous blood cells mbn2		46
ovary fGS/OSS		68
ovary OSC		63
ovary OSS		22
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		151
Cadmium 50 mM 6 hrs, larvae L3		190
Cadmium 50 mM 12 hrs, larvae L3		116
Cadmium 50 mM 48 hrs, 4-day adult		118
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		115
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		152
Ethanol 5% 3 hrs, larvae L3		185
Ethanol 10% 3 hrs, larvae L3		185
Caffeine 1.5 mg/ml 4 hrs, larvae L3		173
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		111
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		137
Paraquat 10 mM 48 hrs, 4-day adult		145
Rotenone 2 μg 12 hrs, larvae L3		226
Rotenone 8 μg 12 hrs, larvae L3		304
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(49)
cold shock, 4-day adult		(39)
heat shock, 4-day adult		(151)
Cadmium 50 mM 6 hrs, larvae L3		(190)
Cadmium 50 mM 12 hrs, larvae L3		(116)
Cadmium 50 mM 48 hrs, 4-day adult		(118)
Cadmium 100 mM 48 hrs, 4-day adult		(77)
Copper 0.5 mM 12 hrs, larvae L3		(115)
Copper 15 mM 48 hrs, 4-day adult		(36)
Zinc 5 mM 12 hrs, larvae L3		(76)
Zinc 4.5 mM 48 hrs, 4-day adult		(41)
Ethanol 2.5% 3 hrs, larvae L3		(152)
Ethanol 5% 3 hrs, larvae L3		(185)
Ethanol 10% 3 hrs, larvae L3		(185)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(173)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(111)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(46)
Paraquat 5 mM 48 hrs, 4-day adult		(137)
Paraquat 10 mM 48 hrs, 4-day adult		(145)
Rotenone 2 μg 12 hrs, larvae L3		(226)
Rotenone 8 μg 12 hrs, larvae L3		(304)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		(151)
Cadmium 50 mM 6 hrs, larvae L3		(190)
Cadmium 50 mM 12 hrs, larvae L3		(116)
Cadmium 50 mM 48 hrs, 4-day adult		(118)
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		(115)
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		(152)
Ethanol 5% 3 hrs, larvae L3		(185)
Ethanol 10% 3 hrs, larvae L3		(185)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(173)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(111)
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		(137)
Paraquat 10 mM 48 hrs, 4-day adult		(145)
Rotenone 2 μg 12 hrs, larvae L3		(226)
Rotenone 8 μg 12 hrs, larvae L3		(304)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		151
Cadmium 50 mM 6 hrs, larvae L3		190
Cadmium 50 mM 12 hrs, larvae L3		116
Cadmium 50 mM 48 hrs, 4-day adult		118
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		115
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		152
Ethanol 5% 3 hrs, larvae L3		185
Ethanol 10% 3 hrs, larvae L3		185
Caffeine 1.5 mg/ml 4 hrs, larvae L3		173
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		111
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		137
Paraquat 10 mM 48 hrs, 4-day adult		145
Rotenone 2 μg 12 hrs, larvae L3		226
Rotenone 8 μg 12 hrs, larvae L3		304
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		151
Cadmium 50 mM 6 hrs, larvae L3		190
Cadmium 50 mM 12 hrs, larvae L3		116
Cadmium 50 mM 48 hrs, 4-day adult		118
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		115
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		152
Ethanol 5% 3 hrs, larvae L3		185
Ethanol 10% 3 hrs, larvae L3		185
Caffeine 1.5 mg/ml 4 hrs, larvae L3		173
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		111
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		137
Paraquat 10 mM 48 hrs, 4-day adult		145
Rotenone 2 μg 12 hrs, larvae L3		226
Rotenone 8 μg 12 hrs, larvae L3		304
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(49)
cold shock, 4-day adult		(39)
heat shock, 4-day adult		(151)
Cadmium 50 mM 6 hrs, larvae L3		(190)
Cadmium 50 mM 12 hrs, larvae L3		(116)
Cadmium 50 mM 48 hrs, 4-day adult		(118)
Cadmium 100 mM 48 hrs, 4-day adult		(77)
Copper 0.5 mM 12 hrs, larvae L3		(115)
Copper 15 mM 48 hrs, 4-day adult		(36)
Zinc 5 mM 12 hrs, larvae L3		(76)
Zinc 4.5 mM 48 hrs, 4-day adult		(41)
Ethanol 2.5% 3 hrs, larvae L3		(152)
Ethanol 5% 3 hrs, larvae L3		(185)
Ethanol 10% 3 hrs, larvae L3		(185)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(173)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(111)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(46)
Paraquat 5 mM 48 hrs, 4-day adult		(137)
Paraquat 10 mM 48 hrs, 4-day adult		(145)
Rotenone 2 μg 12 hrs, larvae L3		(226)
Rotenone 8 μg 12 hrs, larvae L3		(304)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		(151)
Cadmium 50 mM 6 hrs, larvae L3		(190)
Cadmium 50 mM 12 hrs, larvae L3		116
Cadmium 50 mM 48 hrs, 4-day adult		118
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		115
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		(152)
Ethanol 5% 3 hrs, larvae L3		(185)
Ethanol 10% 3 hrs, larvae L3		(185)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(173)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		111
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		137
Paraquat 10 mM 48 hrs, 4-day adult		(145)
Rotenone 2 μg 12 hrs, larvae L3		(226)
Rotenone 8 μg 12 hrs, larvae L3		(304)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		49
cold shock, 4-day adult		39
heat shock, 4-day adult		151
Cadmium 50 mM 6 hrs, larvae L3		190
Cadmium 50 mM 12 hrs, larvae L3		116
Cadmium 50 mM 48 hrs, 4-day adult		118
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		115
Copper 15 mM 48 hrs, 4-day adult		36
Zinc 5 mM 12 hrs, larvae L3		76
Zinc 4.5 mM 48 hrs, 4-day adult		41
Ethanol 2.5% 3 hrs, larvae L3		152
Ethanol 5% 3 hrs, larvae L3		185
Ethanol 10% 3 hrs, larvae L3		185
Caffeine 1.5 mg/ml 4 hrs, larvae L3		173
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		111
Caffeine 25 mg/ml 48 hrs, 4-day adult		46
Paraquat 5 mM 48 hrs, 4-day adult		137
Paraquat 10 mM 48 hrs, 4-day adult		145
Rotenone 2 μg 12 hrs, larvae L3		226
Rotenone 8 μg 12 hrs, larvae L3		304
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0029971

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG18624-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

CG18624^GD15546

viable, with Scer\GAL4^pnr-MD237

CG18624^GD15316

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

mesothoracic tergum, with Scer\GAL4^pnr-MD237

trichogen cell, with Scer\GAL4^pnr-MD237

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG18624	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG18624	Class	Mutagen	Stocks	Known lesion
CG18624^GD15316		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
CG18624^GD15546		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD15316}

P{GD15546}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

NADH dehydrogenase activity

inferred from sequence or structural similarity with UniProtKB:O75438

(Sardiello et al., 2003.6.11)

Biological Process

CV term

Evidence

References

mitochondrial electron transport, NADH to ubiquinone

inferred from sequence or structural similarity with UniProtKB:O75438

(Sardiello et al., 2003.6.11)

Cellular Component

CV term

Evidence

References

mitochondrial respiratory chain complex I

inferred from sequence or structural similarity with UniProtKB:O75438

(Sardiello et al., 2003.6.11)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG18624 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

58171

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0029971

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31697

Orthologs

OrthoDB Orthologs (26) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6HMJX6

Dipteran inclusive ortholog search

EOG6DFPZ7

Insect inclusive ortholog search

EOG6J106D

Arthropod inclusive ortholog search

EOG61RNC7

Metazoa inclusive ortholog search

EOG6QCD2N

Orthologs in Drosophila Species (EOG6HMJX6)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15019

Drosophila persimilis

Dper\GL14582

Drosophila willistoni

Dwil\GK18864

Drosophila virilis

Dvir\GJ16858

Drosophila mojavensis

Dmoj\GI11216

Drosophila grimshawi

Dgri\GH24477

Orthologs in non-Drosophila Dipterans (EOG6DFPZ7)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003423

Anopheles gambiae

Malaria mosquito

Agam\AGAP000849

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015857

Orthologs in non-Dipteran Insects (EOG6J106D)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55150

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011883

Atta cephalotes

Leafcutter ant

Acep\ACEP18237

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO11058

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL11276

Linepithema humile

Argentine ant

Lhum\LH18493

Pogonomyrmex barbatus

Red harvester ant

Pbar\CG:02550629

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI38843

Bombyx mori

Silkmoth

Bmor\BGIBMGA012356

Tribolium castaneum

Red flour beetle

Tcas\TC004001

Orthologs in non-Insect Arthropods (EOG61RNC7)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW006946

Orthologs in non-Arthropod Metazoa (EOG6QCD2N)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007107gn

Danio rerio

Zebrafish

Drer\ENSDARG00000087456

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (9) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila erecta

Dere\GG17601

Drosophila yakuba

Dyak\GE17501

Drosophila ananassae

Dana\GF21240

Drosophila pseudoobscura pseudoobscura

Dpse\GA15019

Drosophila persimilis

Dper\GL14582

Drosophila willistoni

Dwil\GK18864

Drosophila virilis

Dvir\GJ16858

Drosophila mojavensis

Dmoj\GI11216

Drosophila grimshawi

Dgri\GH24477

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v29883

w¹¹¹⁸; P{GD15316}v29883

v29884

w¹¹¹⁸; P{GD15316}v29884

v33892

w¹¹¹⁸; P{GD15546}v33892

v33893

w¹¹¹⁸; P{GD15546}v33893/TM3

Genomic Clones ( 1 )

BACR39C15

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 61 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GM22884

Other clones

TA01431

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0029971

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31697

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG18624 CG15328

(Whitfield, 2000.12.15)

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

31697

UniProtKB/TrEMBL

•

Q9W3N7

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

NADH:ubiquinone oxidoreductase, MNLL subunit (IPR012575)

MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins

•

MTDROME18624

Linkouts

BioGRID - A database of protein and genetic interactions

•

58171

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0029971

DRSC - Results from RNAi screens.

•

FBgn0029971

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0029971

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG18624-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0029971

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31697

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31697

modMine - Data generated by the modENCODE project.

•

FBgn0029971

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

CG15328

CG18624

(Vilain et al., 2012, Porcelli et al., 2007, Tripoli et al., 2005, Larschan et al., 2007, Hayden and Bosco, 2008)

Dromel_CG18624_FBtr0071140_mORF

(Hayden and Bosco, 2008)

Name Synonym

NADH:ubiquinone oxidoreductase MNLL subunit

(Tripoli et al., 2005)

NADH ubiquinone oxidoreductase MNLL

(Porcelli et al., 2007)

Secondary FlyBase IDs

FBgn0040924

References ( 15 )

Research paper

Vilain et al., 2012, PLoS Genet. 8(1): e1002456: The Yeast Complex I Equivalent NADH Dehydrogenase Rescues pink1 Mutants. [FBrf0217195]
Hayden and Bosco, 2008, BMC Genomics 9: 61: Comparative genomic analysis of novel conserved peptide upstream open reading frames in Drosophila melanogaster and other dipteran species. [FBrf0204849]
Porcelli et al., 2007, BMC Evol. Biol. 7: 215: The nuclear OXPHOS genes in insecta: a common evolutionary origin, a common cis-regulatory motif, a common destiny for gene duplicates. [FBrf0204833]
Tripoli et al., 2005, Genome Biol. 6(2): R11: Comparison of the oxidative phosphorylation (OXPHOS) nuclear genes in the genomes of Drosophila melanogaster, Drosophila pseudoobscura and Anopheles gambiae. [FBrf0184015]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
Khanna et al., 2010, BMC Genomics 11(1):: Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane. [FBrf0210978]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Hayden and Bosco, 2008, BMC Genomics:: File 1: Conserved peptide uORF and associated mORF amino acid sequences in Drosophila melanogaster. [FBrf0205942]
Larschan et al., 2007, Mol. Cell 28(1):: Supplemental Data. [FBrf0207861]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Sardiello et al., 2003.6.11, MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion.: MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion. [FBrf0159903]
Whitfield, 2000.12.15, Deleted protein_ids - part 1.: Deleted protein_ids - part 1. [FBrf0132190]