dMLK, MLK2, MLK, JNKKK
mixed lineage kinase - signal transduction protein conveying information about changes in cytoskeleton to the nucleus - regulates dorsal closure - controls cell fate and cell sheet morphogenesis during embryogenesis
Please see the JBrowse view of Dmel\slpr for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
Gene model reviewed during 5.56
5-6 (northern blot)
1148 (aa)
Homodimer.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\slpr using the Feature Mapper tool.
Comment: 1-3 hr AEL
Transcript was detected strongly in 0-4 h embryos suggesting that it was maternally deposited. Expression steadily decreased throughout embryogenesis until no transcript was detectable by stage 16. Expression was also detected in the adult head and thorax but not the abdomen.
GBrowse - Visual display of RNA-Seq signals
View Dmel\slpr in GBrowse 21-22
1-22.3
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Mlk2 CG2272
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi experiments show that dsRNA targetted at this gene suppresses the Pten-dsRNA-induced cell shape changes in Kc167 cells.
The kinase activity of slpr protein can be activated by C6-ceramide or sphingomyelinase in vivo, and by nM concentrations of natural ceramide in vitro.
slpr encodes a Jun kinase kinase kinase.
Mutant embryos have a "dorsal-open" phenotype. The leading edge cells of the epidermis stretch transiently in the dorso-ventral axis, but the elongation is not maintained and the epidermis ultimately retracts.
The gene is named "slipper" after the mutant phenotype; embryonic cuticles resemble an open shoe.
The gene is named after the cuticles of mutant embryos, which resemble an open shoe, like a ballet slipper.
