FlyBase Gene Report: Dmel\CCT2

General Information

Symbol

Dmel\CCT2

Species

D. melanogaster

Name

Chaperonin containing TCP1 subunit 2

Annotation Symbol

CG7033

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030086

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

8C8-8C8

Sequence location

X:9,089,513..9,092,826 [+]

Recombination map

1-26

RefSeq locus

NC_004354 REGION:9089513..9092826

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR002423, InterPro:IPR012716

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR002194, InterPro:IPR002423, InterPro:IPR012716

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATP-dependent protein folding chaperone

inferred from electronic annotation with InterPro:IPR017998

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables unfolded protein binding

inferred from biological aspect of ancestor with PANTHER:PTN004253040

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in protein folding

inferred from biological aspect of ancestor with PANTHER:PTN004253040

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of chaperonin-containing T-complex

inferred from biological aspect of ancestor with PANTHER:PTN000144135

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CHAPERONIN CONTAINING TCP-1 COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CHAPERONIN CONTAINING TCP-1 COMPLEX -: Group II Heat Shock Protein 60 chaperonins are paralogous subunits that assemble to form a multi-subunit ring complex, the TCP-1 Ring Complex (TRiC) or Chaperonin Containing TCP-1 (CCT) complex. Within the cytoplasm, TRiC/CCT catalyses the ATP-dependent folding of approximately 10% of of newly synthesized proteins. (Adapted from FBrf0210519, FBrf0232269 and PMID:11580267).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CCT2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W392)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CCT2-RA

FBtr0071289

NM_132296

2121

535

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CCT2-PA

FBpp0071226

58.1

535

5.59

Q9W392

NP_572524

AAF46442

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Q9W392

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CCT2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG7033_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic anal pad

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval dorsal vessel

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CCT2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CCT2

View exonic expression by tissue for Dmel\CCT2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030086

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31838/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030086

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030086

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030086

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7033

Flygut - An atlas of the Drosophila adult midgut

FBgn0030086

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CCT2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CCT2

Transgenic constructs containing regulatory region of CCT2

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

CCT2^GD5201

lethal, with Scer\GAL4^pnr-MD237

CCT2^GD5201

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

CCT2^GD5201

lethal - all die during pharate adult stage, with Scer\GAL4^ey.PU

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CCT2^GD5201

viable, with Scer\GAL4^tin.CΔ4

CCT2^GD5201

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage

CCT2^GD5201

abnormal neuroanatomy | larval stage, with Scer\GAL4^wor.PA

CCT2^GD5201

visible | pharate adult stage, with Scer\GAL4^ey.PU

Phenotype manifest in

Allele

adult head | pharate adult stage, with Scer\GAL4^ey.PU

ganglion mother cell | larval stage

CCT2^GD5201

larval neuroblast | larval stage

CCT2^GD5201

neuroblast | larval stage, with Scer\GAL4^wor.PA

CCT2^GD5201

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CCT2

14 of 15

Yes

Hsap\CCT7

2 of 15

Hsap\TCP1

2 of 15

Hsap\BBS10

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\VKORC1L1

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cct2

15 of 15

Yes

Mmus\Tcp1

2 of 15

Mmus\Bbs10

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (11)

Rnor\Cct2

13 of 13

Yes

Rnor\Cct7

2 of 13

Rnor\Tcp1

2 of 13

Rnor\Bbs10

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (8)

Xtro\cct2

9 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (10)

Drer\cct2

7 of 15

Yes

Drer\cct7

2 of 15

Drer\tcp1

2 of 15

Drer\bbs10

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\cct-2

13 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\AT5G20890

9 of 9

Yes

Atha\AT3G11830

1 of 9

Atha\TCP-1

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\CCT2

14 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cct2

11 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (13)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CCT2; chaperonin containing TCP1 subunit 2

14 of 15

605139

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CCT2 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT2 - CCT1

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT4

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT5

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT7

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - CG1416

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Chc

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Hecw

pull down, Identification by mass spectrometry

(Fajner et al., 2021)

CCT2 - Hsp23

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Kap-α3

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

CCT2 - Rop

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

CCT2 - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT2 - hpo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

CCT2 - mst

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

CCT2 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CCT2 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CCT2

suppressible

fzy

(Kuang et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58293

DroID - A comprehensive database of gene and protein interactions.

FBgn0030086

MIST (genetic) - An integrated Molecular Interaction Database

CG7033

MIST (protein-protein) - An integrated Molecular Interaction Database

CG7033

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0030086

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-26

Cytogenetic map

8C8-8C8

Sequence location

X:9,089,513..9,092,826 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8C8-8C8

Limits computationally determined from genome sequence between P{EP}CG10962^EP1565&P{EP}EP1030 and P{EP}EP1450&P{EP}EP1356

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-26

(FlyBase, 2016)

1-27.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

53755

w^*; P{UASp-CCT2.EGFP.C}3

53754

w^*; P{UASp-CCT2.EGFP.C}2/CyO

26627

w^* P{EP}CCT2^G11254/FM6, w^*

34711

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01190}attP2

FlyORF

F001602

M{UAS-CCT2.ORF}ZH-86Fb

F001042

M{UAS-CCT2.ORF.3xHA}ZH-86Fb

VDRC

v108615

P{KK101795}VIE-260B

v41190

w¹¹¹⁸; P{GD5201}v41190

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (75)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD02216

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030086

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030086

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31838

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: CCT2 CG7033

(Monzo et al., 2010)

Source for database merge of

Additional comments

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31838

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W392

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9W392

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.