Gene Dmel\Vago

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General Information

Symbol

Dmel\Vago

Species

D. melanogaster

Name

Vago

Annotation symbol

CG2081

Feature type

protein_coding_gene

FlyBase ID

FBgn0030262

Gene Model Status

Current

Stock availability

1 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

10A4-10A4

Sequence location

X:10,983,323..10,984,140 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Vago is referred to in FlyBase by the symbol Dmel\Vago (CG2081, FBgn0030262). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: defense response to virus. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval hindgut, adult heart, virgin spermatheca, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639476_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Vago. Gene sequence location is X:10983323..10984140.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

10A4-10A4

Limits computationally determined from genome sequence between P{EP}EP1321^EP1321&P{EP}CG2061^EP1537 and P{EP}CG11756^EP1610

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Vago for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Vago-RB

FBtr0073427

NM_132452

684

160

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Vago-PB

FBpp0073283

18.1

160

5.59

NP_572680

AAF47993

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval hindgut, adult heart, virgin spermatheca, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		3145.9
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		(3145.9)
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		(859.8)
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		(596.1)
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		(732)
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		(3145.9)
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		3145.9
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		3145.9
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		(3145.9)
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		(859.8)
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		(3145.9)
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		5.7
Larval Midgut		27.4
Larval Hindgut		100.4
Larval Malpighian Tubules		10.9
Larval Fat Body		3145.9
Larval Salivary Gland		30.9
Larval Trachea		23.6
Larval Carcass		20.125
Adult Head		354.9
Adult Eye		136.775
Adult Brain		9.8
Adult Thoracic-Abdominal Ganglion		6.9
Adult Crop		859.8
Adult Midgut		3
Adult Hindgut		77.2
Adult Malpighian Tubules		6
Adult Fat Body		596.1
Adult Salivary Gland		2.9
Adult Heart		358.25
Adult VirginFemale Spermatheca		280.3
Adult InseminatedFemale Spermatheca		732
Adult Ovary		0.7
Adult Testis		0.8
Adult Male Accessory Gland		7.7
Adult Carcass		294.6
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	354.9
NA	Eye	136.775
NA	Brain	9.8
5.7	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	6.9
NA	Crop	859.8
no informative data	Midgut	3
100.4	Hindgut	77.2
no informative data	Malpighian Tubules	6
3145.9	Fat Body	596.1
30.9	Salivary Gland	2.9
NA	Heart	358.25
no informative data	Trachea	NA
NA	VirginFemale Spermatheca	280.3
NA	InseminatedFemale Spermatheca	732
NA	Ovary	0.7
NA	Testis	0.8
NA	Male Accessory Gland	7.7
no informative data	Carcass	294.6

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		(31)
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		(95)
fat body, white prepupae		(42)
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		(95)
fat body, white prepupae		(42)
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		9
head, virgin 4-day female		11
head, virgin 20-day female		31
head, mated 1-day female		14
head, mated 4-day female		16
head, mated 20-day female		25
head, mated 1-day male		16
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		95
fat body, white prepupae		42
fat body, pupae P8		0
carcass, larvae L3 wandering		3
carcass, 1-day adult		8
carcass, 4-day adult		7
carcass, 20-day adult		11
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed during late larval stages, during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		(37)
larva L3 puffstage 1-2		(85)
larva L3 puffstage 3-6		(75)
larva L3 puffstage 7-9		(50)
white prepupae new		(58)
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		(52)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		(37)
larva L3 puffstage 1-2		(85)
larva L3 puffstage 3-6		(75)
larva L3 puffstage 7-9		(50)
white prepupae new		(58)
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		(52)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		2
embryo 16-18hr		12
embryo 18-20hr		17
embryo 20-22hr		7
embryo 22-24hr		7
larva L1		7
larva L2		17
larva L3 12hr old		37
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		50
white prepupae new		58
white prepupae 12hr		16
white prepupae 24hr		12
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		10
adult male 01day		14
adult male 05day		12
adult male 30day		15
adult female 01day		22
adult female 05day		18
adult female 30day		52
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		(49)
ovary OSC		(39)
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		(49)
ovary OSC		(39)
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		1
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		1
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		1
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		9
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		0
ovary fGS/OSS		49
ovary OSC		39
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		(79)
Ethanol 10% 3 hrs, larvae L3		(32)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(42)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		(79)
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(42)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		6
heat shock, 4-day adult		11
Cadmium 50 mM 6 hrs, larvae L3		15
Cadmium 50 mM 12 hrs, larvae L3		22
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		4
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		10
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		10
Ethanol 5% 3 hrs, larvae L3		79
Ethanol 10% 3 hrs, larvae L3		32
Caffeine 1.5 mg/ml 4 hrs, larvae L3		42
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		18
Rotenone 8 μg 12 hrs, larvae L3		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_04

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030262

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG2081-RB

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Vago^ΔM10

viable, with Scer\GAL4^pnr-MD237

Vago^GD4010

Sterility

Allele

fertile

Vago^ΔM10

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Vago	Class	Mutagen	Stocks	Known lesion
Vago^ΔM10		Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Vago	Class	Mutagen	Stocks	Known lesion
Vago^GD4010		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Vago^Scer\UAS.cDa		in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)Vago^ΔM11

(Deddouche et al., 2008)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{Vago-lacZ.1.9}

UAS construct

P{GD4010}

P{UAS-Vago.D}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}GE2537

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

defense response to virus

inferred from mutant phenotype

(Deddouche et al., 2008)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

extracellular space

inferred from sequence model

(Sheldon et al., 2007)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Vago allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0030262

Orthologs

OrthoDB Orthologs (11) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6KPTXC

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6KPTXC)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15228

Drosophila persimilis

Dper\GL18255

Drosophila virilis

Dvir\GJ15634

Drosophila mojavensis

Dmoj\GI16062

Drosophila grimshawi

Dgri\GH17919

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15228

Drosophila persimilis

Dper\GL18255

Drosophila virilis

Dvir\GJ15634

Drosophila mojavensis

Dmoj\GI16062

Drosophila grimshawi

Dgri\GH17919

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v13597

w¹¹¹⁸ P{GD4010}v13597

Genomic Clones ( 1 )

BACR26J12

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 10 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RH70969

Other clones

GH22911

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0030262

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32040

Antibody Information

Other Information

Discoverer

Etymology

Named "Vago" after the French pioneer of the study of insect-virus interactions, Constantin Vago, as expression of the gene is upregulated in response to Drosophila C virus infection.

(Imler, 2006.3.8)

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Vago CG2081

(Imler, 2006.3.8)

Source for database merge of

Source for merge of: CG2081 BcDNA:RH70969

(FlyBase, 1996-)

Additional comments

Source for merge of CG2081 BcDNA:RH70969 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_132452

RefSeq (Proteins)

•

NP_572680

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

32040

UniProtKB/TrEMBL

•

Other Crossreferences

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0030262

DRSC - Results from RNAi screens.

•

FBgn0030262

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030262

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG2081-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0030262

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32040

modMine - Data generated by the modENCODE project.

•

FBgn0030262

Synonyms & Secondary IDs ( 4 )

Reported As

Symbol Synonym

BcDNA:RH70969

CG2081

(Dostert et al., 2005, FlyBase, 1996-, Sheldon et al., 2007, Deddouche et al., 2008, Cammarato et al., 2011)

DIGF-2

(Kleinjung et al., 2004)

Vago

(Rynes et al., 2012, Vatolina et al., 2011, Deddouche et al., 2008, Sheldon et al., 2007, Deddouche et al., 2008, Babenko et al., 2009, Carpenter et al., 2009, Zsindely et al., 2009)

Name Synonym

Vago

(Imler, 2006.3.8, Cammarato et al., 2011)

Secondary FlyBase IDs

FBgn0062908
FBpp0073283
FBtr0073427

References ( 18 )

Research paper

Rynes et al., 2012, Mol. Cell. Biol. 32(19): 3949--3962: Activating transcription factor 3 regulates immune and metabolic homeostasis. [FBrf0219385]
Vatolina et al., 2011, PLoS ONE 6(10): e25960: Identical Functional Organization of Nonpolytene and Polytene Chromosomes in Drosophila melanogaster. [FBrf0216448]
Babenko et al., 2009, Dokl. Biochem. Biophys. 424: 27--30: Characteristics of molecular-genetic organization of intercalary heterochromatin band 10A1-2 in X chromosome of Drosophila melanogaster. [FBrf0207731]
Carpenter et al., 2009, PLoS ONE 4(8): e6838: The transcriptional response of Drosophila melanogaster to infection with the sigma virus (Rhabdoviridae). [FBrf0208625]
Zsindely et al., 2009, Nucleic Acids Res. 37(20): 6665--6680: The loss of histone H3 lysine 9 acetylation due to dSAGA-specific dAda2b mutation influences the expression of only a small subset of genes. [FBrf0209301]
Deddouche et al., 2008, Nat. Immunol. 9(12): 1425--1432: The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila. [FBrf0206193]
Sheldon et al., 2007, FEBS Lett. 581(27): 5268--5274: A novel family of single VWC-domain proteins in invertebrates. [FBrf0200806]
Dostert et al., 2005, Nat. Immunol. 6(9): 946--953: The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of Drosophila. [FBrf0188155]
Kleinjung et al., 2004, Nucleic Acids Res. 32(8): 2464--2473: Contact-based sequence alignment. [FBrf0175042]

Supplementary material

Cammarato et al., 2011, PLoS ONE 6(4):: Table S1. Proteins & Peptides. [FBrf0214682]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Imler, 2006.3.8, [title not yet available]: [title not yet available] [FBrf0191757]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

Abstract

Deddouche et al., 2008, A. Dros. Res. Conf. 49: 755B: Dicer-2 mediated inducible antiviral response in drosophila. [FBrf0203618]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]