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Gene Dmel\rho-4

General Information
Symbol	Dmel\rho-4	Species	D. melanogaster
Name	rhomboid-4	Annotation symbol	CG1697
Feature type	protein_coding_gene	FlyBase ID	FBgn0030318
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	rho4
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	10C10-10C10	Sequence location	X:11,493,874..11,500,034 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene rhomboid-4 is referred to in FlyBase by the symbol Dmel\rho-4 (CG1697, FBgn0030318). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: serine-type peptidase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: proteolysis. 11 alleles are reported. The phenotype of these alleles is annotated with: wing. It has one annotated transcript and one annotated polypeptide. Protein features are: Calcium-binding EF-hand; EF-HAND 2; EF-Hand 1, calcium-binding site; EF-hand-like domain; Peptidase S54, rhomboid; Peptidase S54, rhomboid domain; Peptidase S54, rhomboid, metazoan. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1623692_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of rho-4. Gene sequence location is X:11493874..11500034.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features HFA19762 HFA19761 BKN25738
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 10C10-10C10
Experimentally Determined Cytological Location
Cytogenetic map Notes References 10C9-10C9     (Freeman, 2000.11.2)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\rho-4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) rho-4-RA FBtr0073519  NM_167290   2055   417
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein rho-4-PA   FBpp0073366   48.0   417   5.84     NP_727538   AAF48067
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • EF-Hand 1, calcium-binding site (IPR018247) EF-HAND 2 (IPR018249) Calcium-binding EF-hand (IPR002048) Peptidase S54, rhomboid (IPR002610) EF-hand-like domain (IPR011992) Peptidase S54, rhomboid, metazoan (IPR017213) Peptidase S54, rhomboid domain (IPR022764)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246293   AA392488   AA950839   AA950869   AA978803   AE014298 AAF48067   AF318285 AAK06754   AI109619   AI257572   AI259757   AI455078   AI455654   AI456316   AI456883   AI457071   AI514814   AI516245   AI516765   AI517912   AI520315   AI520325   AI534564   AI541770   AW944477   AY061605 AAL29153   BH854672   BI163216   BI166697   BI166882   BI170547   BI213997   BI215220   BI235452   BI235724   BI237397   BI237650   BI237901   BI237993   BI238121   BI238446   BI239292   BI239872   BI361851   BI366467   BI368670   BI370250   BI370822   BI481568   BI482015   BI482970   BI483856   BI484382   BI485917   BI579284   BI579325   BI579343   BI580518   BI632179   BI632908   BI635456   BP546061   BP556528   BP556529   BP558343   BP558344   BQ103175   CL527878   CO153452   CO177984   CO268758   CO289126   CO289942   CO297328   CO300485   CO309230   CZ472454   CZ480258   EC066363   EC071089   EC081118   EC196294   EC199930   EC219953   EC230758   EC236125   EV594215
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VYW6 Q0KHT6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0030318    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0030318 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0030318 expression level Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3015) embryo 02-04hr  (4777) embryo 04-06hr  (4321) embryo 06-08hr  (3817) embryo 08-10hr  (3272) embryo 10-12hr  (2509) embryo 12-14hr  (2556) embryo 14-16hr  (2673) embryo 16-18hr  (1587) embryo 18-20hr  (1123) embryo 20-22hr  (2685) embryo 22-24hr  (2336) larva L1  (1238) larva L2  (1825) larva L3 12hr old  (904) larva L3 puffstage 1-2  (1395) larva L3 puffstage 3-6  (2014) larva L3 puffstage 7-9  (3153) white prepupae new  (3363) white prepupae 12hr  (2216) white prepupae 24hr  (2264) pupae 2d postWPP  (2223) pupae 3d postWPP  (898) pupae 4d postWPP  (608) adult male 01day  (699) adult male 05day  (713) adult male 30day  (775) adult female 01day  (1514) adult female 05day  (1631) adult female 30day  (1852) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3015) embryo 02-04hr  (4777) embryo 04-06hr  (4321) embryo 06-08hr  (3817) embryo 08-10hr  (3272) embryo 10-12hr  (2509) embryo 12-14hr  (2556) embryo 14-16hr  (2673) embryo 16-18hr  (1587) embryo 18-20hr    1123 embryo 20-22hr  (2685) embryo 22-24hr  (2336) larva L1    1238 larva L2  (1825) larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6  (2014) larva L3 puffstage 7-9  (3153) white prepupae new  (3363) white prepupae 12hr  (2216) white prepupae 24hr  (2264) pupae 2d postWPP  (2223) pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day  (1514) adult female 05day  (1631) adult female 30day  (1852) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0030318 expression level Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3015) embryo 02-04hr  (4777) embryo 04-06hr  (4321) embryo 06-08hr  (3817) embryo 08-10hr  (3272) embryo 10-12hr  (2509) embryo 12-14hr  (2556) embryo 14-16hr  (2673) embryo 16-18hr  (1587) embryo 18-20hr  (1123) embryo 20-22hr  (2685) embryo 22-24hr  (2336) larva L1  (1238) larva L2  (1825) larva L3 12hr old  (904) larva L3 puffstage 1-2  (1395) larva L3 puffstage 3-6  (2014) larva L3 puffstage 7-9  (3153) white prepupae new  (3363) white prepupae 12hr  (2216) white prepupae 24hr  (2264) pupae 2d postWPP  (2223) pupae 3d postWPP  (898) pupae 4d postWPP  (608) adult male 01day  (699) adult male 05day  (713) adult male 30day  (775) adult female 01day  (1514) adult female 05day  (1631) adult female 30day  (1852) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3015) embryo 02-04hr  (4777) embryo 04-06hr  (4321) embryo 06-08hr  (3817) embryo 08-10hr  (3272) embryo 10-12hr  (2509) embryo 12-14hr  (2556) embryo 14-16hr  (2673) embryo 16-18hr  1587 embryo 18-20hr    1123 embryo 20-22hr  (2685) embryo 22-24hr  2336 larva L1    1238 larva L2  1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6  2014 larva L3 puffstage 7-9  (3153) white prepupae new  (3363) white prepupae 12hr  2216 white prepupae 24hr  2264 pupae 2d postWPP  2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day  1514 adult female 05day  1631 adult female 30day  1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3015 embryo 02-04hr    4777 embryo 04-06hr    4321 embryo 06-08hr    3817 embryo 08-10hr    3272 embryo 10-12hr    2509 embryo 12-14hr    2556 embryo 14-16hr    2673 embryo 16-18hr    1587 embryo 18-20hr    1123 embryo 20-22hr    2685 embryo 22-24hr    2336 larva L1    1238 larva L2    1825 larva L3 12hr old    904 larva L3 puffstage 1-2    1395 larva L3 puffstage 3-6    2014 larva L3 puffstage 7-9    3153 white prepupae new    3363 white prepupae 12hr    2216 white prepupae 24hr    2264 pupae 2d postWPP    2223 pupae 3d postWPP    898 pupae 4d postWPP    608 adult male 01day    699 adult male 05day    713 adult male 30day    775 adult female 01day    1514 adult female 05day    1631 adult female 30day    1852 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0030318    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0030318 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0030318 expression level Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0030318 expression level Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    151.4 Larval Midgut    463.2 Larval Hindgut    343.5 Larval Malpighian Tubules    330 Larval Fat Body    70.6 Larval Salivary Gland    260.7 Larval Trachea    244.075 Larval Carcass    193.2 Adult Head    59.9 Adult Eye    49.875 Adult Brain    51.4 Adult Thoracic-Abdominal Ganglion    47.9 Adult Crop    290.1 Adult Midgut    378.9 Adult Hindgut    239.5 Adult Malpighian Tubules    144.4 Adult Fat Body    53.1 Adult Salivary Gland    176.7 Adult Heart    36.875 Adult VirginFemale Spermatheca    163 Adult InseminatedFemale Spermatheca    215.7 Adult Ovary    211.9 Adult Testis    61.7 Adult Male Accessory Gland    97.8 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    59.9   NA  Eye    49.875   NA  Brain    51.4   151.4  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    47.9   NA  Crop    290.1   463.2  Midgut    378.9   343.5  Hindgut    239.5   330  Malpighian Tubules    144.4   70.6  Fat Body    53.1   260.7  Salivary Gland    176.7   NA  Heart    36.875   244.075  Trachea    NA   NA  VirginFemale Spermatheca    163   NA  InseminatedFemale Spermatheca    215.7   NA  Ovary    211.9   NA  Testis    61.7   NA  Male Accessory Gland    97.8   193.2  Carcass    71.7 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030318 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • Transposon.85;CG1697-RC;Transposon.85
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable rho-4BG01732 rho-4EY06222 viable, with Scer\GAL4pnr-MD237 rho-4GD3543 Sterility Allele fertile rho-4BG01732 rho-4EY06222 Phenotype manifest in Allele wing, with Scer\GAL4Bx-MS1096 rho-4Scer\UAS.cUa wing vein | ectopic, with Scer\GAL4Bx-MS1096 rho-4Scer\UAS.cUa
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of rho-4 Class Mutagen Stocks Known lesion rho-45-HA-1976 1 -- rho-4BG01732 1 -- rho-4CB-5281-3 1 -- rho-4d01806 P-element activity 1 -- rho-4e02443 piggyBac transposase 1 -- rho-4EY06222 P-element activity 1 -- rho-4Δ 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of rho-4 Class Mutagen Stocks Known lesion rho-4GD3543 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes rho-4KK104778 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rho-4dsRNA.cUa in vitro construct - RNAi 0 Yes rho-4Scer\UAS.cUa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3543} NA P{KK104778} NA P{UAS-rho-4.U} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}CG2533BG01732 NA P{RS3}rho-4CB-5281-3 NA P{RS5}rho-45-HA-1976 NA P{SUPor-P}KG07606 NA PBac{RB}rho-4e02443 NA insertion of mobile activating element P{EPgy2}rho-4EY06222 NA P{GSV1}EP-ZEP-Z NA P{XP}rho-4d01806 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
calcium ion binding inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992 (FlyBase Curators et al., 2004-) serine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR002610, InterPro:IPR017213, InterPro:IPR022764 (FlyBase Curators et al., 2004-) serine-type peptidase activity non-traceable author statement (Urban, 2001.10.16)
Biological Process
CV term Evidence References
proteolysis inferred from electronic annotation with InterPro:IPR002610 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR002610, InterPro:IPR017213, InterPro:IPR022764 (FlyBase Curators et al., 2004-) non-traceable author statement (Urban, 2001.10.16) plasma membrane non-traceable author statement (Freeman, 2000.11.2)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions rho-4 allele Gene References rho-4Scer\UAS.cUa S (Urban et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58518 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0030318 DroID - A comprehensive database of gene and protein interactions. • FBgn0030318 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32109
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21300 Dere\GG18433 Dgri\GH24104 Dmoj\GI14374 Dper\GL26733 Dpse\GA14244 Dsec\GM13096 Dsim\GD17034 Dvir\GJ19413 Dwil\GK15930 Dyak\GE15951
Curated drosophilid orthologs	Dpse\GA14244
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL009681-PA Aedes aegypti (mosquito) AGAP004737-PA Anopheles gambiae (mosquito) XP_395780.2 Apis mellifera (honey bee) XP_001947411.1 Acyrthosiphon pisum (pea aphid) XP_001896728.1 Brugia malayi (roundworm nematode) BGIBMGA013057-PA Bombyx mori (silk worm) JA15067 Caenorhabditis japonica (nematode) CPIJ002510 Culex pipens (mosquito) 169308 Capitella spl (polychaete worm) 312433 Daphnia pulex (daphnia) XP_651687 Entamoeba histolytica (anaerobic protozoan) 109972 Hypsipyla robusta (cedar shoot borer) ISCW021997-PA Ixodes scapularis (deer tick) 230044 Lottia gigantea (owl limpet) XP_001606093.1 Nasonia vitripennis (wasp) LOC_Os04g48130.1 Oryza sativa (asian rice) LOC_Os08g43320.1 Oryza sativa (asian rice) 11016 Ostreococcus tauri (green alga) PHUM003375-PA Pediculus humanus (human body louse) PP06693 Pristionchus pacificus (nematode) 73984 Phytophthora ramorum (sudden oak death pathogen) 87592 Phytophthora ramorum (sudden oak death pathogen) Smp_020090 Schistosoma mansoni (trematode parasite) XP_972541.1 Tribolium castaneum (red flour beetle) TP02_0562 Theileria parva (parasitic protozoan) 85781.m00157 Trichomonas vaginalis (parasitic flagellated protozoan) 89678.m00174 Trichomonas vaginalis (parasitic flagellated protozoan) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15673 LH19243 ISCW009902 FBgn0233507 FBgn0217932 FBgn0206556 FBgn0188608 FBgn0168027 JGI_V11_312433 FBgn0074273 FBgn0164314 FBgn0137126 FBgn0131560 FBgn0110648 FBgn0098302 CPIJ002510 BGIBMGA013057 ACYPI003365 GB16638 AGAP004737 AAEL009681 PB22097 PHUM240580 GLEAN_06133 GLEAN_15513
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	16393 y1 w67c23 P{EPgy2}rho-4EY06222 12564 w1118 P{GT1}CG2533BG01732 rho-4BG01732
Harvard	- P{XP}rho-4d01806 - PBac{RB}rho-4e02443
Kyoto	123547 w1118 P{RS3}rho-4CB-5281-3 125505 w1118 P{RS5}rho-45-HA-1976
VDRC	v100200 P{KK104778}VIE-260B v44414 w1118; P{GD3543}v44414/TM3 v44416 w1118; P{GD3543}v44416/TM3
Genomic Clones ( 2 )
	BACR26B05 BACR29A04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 77 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD06923
Other clones	DPiM_SD06923
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH08858 LD05255 LD11363 LD18094 LD30983 LD31022 LD32965 LD35185 LD36233 LD37044 LD37306 LD40440 LD40452 LD42043 LD42815 LD43052 LD46513 LP03244 LP05976 RE02559 RE06895 RE07123 RE12052 RE19672 RE21562 RE31294 RE31643 RE33621 RE33913 RE34233 RE34336 RE34509 RE34917 RE35748 RE36479 RE46233 RE52004 RE54677 RE56706 RE57578 RE64143 RE64655 RE65781 RE66812 RE67472 RE69274 RE73286 RE73342 RE73362 RE74725 SD07459 SD16821 SD25805 SD26810
Other clones	53717 79403 158145 CK01.68C.D5 CK01.89B.H4 CK01.93A.G9 CK01.120A.C11 CK01.121A.E1 Dmel_neb_014175_0616_3159 EK054915 EK070147 EK072627 EK092617 EK178313 EK216707 EK260762 EN02008 FGH102D08 FGM304A10 FGM314D10 UUGC0573
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0030318 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32109
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: CG1697 rho-4 (Wasserman et al., 2000)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_167290 RefSeq (Proteins) • NP_727538 DDBJ / EMBL / GenBank • AAF48067 AAG22342 AAK06754 AAL29153 AAN09637 AF318285 AI109619 AI541770 AW944477 AY061605 BH854672 CL527878 CZ472454 CZ480258 Entrez Gene - A searchable database of RefSeq genes. • 32109 UniProtKB/TrEMBL • Q0KHT6 Q9VYW6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • EF-Hand 1, calcium-binding site (IPR018247) EF-HAND 2 (IPR018249) Calcium-binding EF-hand (IPR002048) Peptidase S54, rhomboid (IPR002610) EF-hand-like domain (IPR011992) Peptidase S54, rhomboid, metazoan (IPR017213) Peptidase S54, rhomboid domain (IPR022764)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58518 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0030318 DroID - A comprehensive database of gene and protein interactions. • FBgn0030318 DRSC - Results from RNAi screens. • FBgn0030318 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030318 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • Transposon.85;CG1697-RC;Transposon.85 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030318 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32109 InParanoid A subset of ortholog calls from InParanoid. • AAEL009681-PA Aedes aegypti (mosquito) AGAP004737-PA Anopheles gambiae (mosquito) XP_395780.2 Apis mellifera (honey bee) XP_001947411.1 Acyrthosiphon pisum (pea aphid) XP_001896728.1 Brugia malayi (roundworm nematode) BGIBMGA013057-PA Bombyx mori (silk worm) JA15067 Caenorhabditis japonica (nematode) CPIJ002510 Culex pipens (mosquito) 169308 Capitella spl (polychaete worm) 312433 Daphnia pulex (daphnia) XP_651687 Entamoeba histolytica (anaerobic protozoan) 109972 Hypsipyla robusta (cedar shoot borer) ISCW021997-PA Ixodes scapularis (deer tick) 230044 Lottia gigantea (owl limpet) XP_001606093.1 Nasonia vitripennis (wasp) LOC_Os04g48130.1 Oryza sativa (asian rice) LOC_Os08g43320.1 Oryza sativa (asian rice) 11016 Ostreococcus tauri (green alga) PHUM003375-PA Pediculus humanus (human body louse) PP06693 Pristionchus pacificus (nematode) 73984 Phytophthora ramorum (sudden oak death pathogen) 87592 Phytophthora ramorum (sudden oak death pathogen) Smp_020090 Schistosoma mansoni (trematode parasite) XP_972541.1 Tribolium castaneum (red flour beetle) TP02_0562 Theileria parva (parasitic protozoan) 85781.m00157 Trichomonas vaginalis (parasitic flagellated protozoan) 89678.m00174 Trichomonas vaginalis (parasitic flagellated protozoan) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32109 modMine - Data generated by the modENCODE project. • FBgn0030318 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15673 LH19243 ISCW009902 FBgn0233507 FBgn0217932 FBgn0206556 FBgn0188608 FBgn0168027 JGI_V11_312433 FBgn0074273 FBgn0164314 FBgn0137126 FBgn0131560 FBgn0110648 FBgn0098302 CPIJ002510 BGIBMGA013057 ACYPI003365 GB16638 AGAP004737 AAEL009681 PB22097 PHUM240580 GLEAN_06133 GLEAN_15513
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG1697 (Molnar et al., 2006, Keleman et al., 2009.8.5, Chang et al., 2008) Rho-4 (Urban et al., 2002) rho-4 (Molnar et al., 2006, FlyBase, 1992-, Chang et al., 2008, Straub et al., 2008, Zeitlinger et al., 2007, Yu et al., 2010, Kong et al., 2010) rho 4 (Yu et al., 2010) rho4 (Yu et al., 2010, Jiang et al., 2011) Rho-related [10C6] (Guichard et al., 2000)
Name Synonym	rhomboid-4 (Wasserman et al., 2000, Chang et al., 2008) Rhomboid-4 (Urban, 2006) Rhomboid 4 (Buchon et al., 2009, Kong et al., 2010)
Secondary FlyBase IDs
References ( 28 )
Generate a list of	All references relating to this gene ( 28 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 )
Recent research papers ( 3 )
	Jiang et al., 2011, Cell Stem Cell 8(1): 84--95 EGFR/Ras/MAPK Signaling Mediates Adult Midgut Epithelial Homeostasis and Regeneration in Drosophila. [FBrf0212694] Kong et al., 2010, Alcoholism, Clin. Exp. Res. 34(2): 302--316 Ethanol-regulated genes that contribute to ethanol sensitivity and rapid tolerance in Drosophila. [FBrf0209720] Yu et al., 2010, PLoS Genet. 6(5): e1000969 Affecting Rhomboid-3 function causes a dilated heart in adult Drosophila. [FBrf0210954] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...