FlyBase Gene Report: Dmel\Tango4

SPLICEOSOME COMPLEX B -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. After the assembly of complex A on pre-mRNA, the U4-U6 and U5 snRNPs are recruited as a preassembled tri-snRNP to form complex B. U5 snRNP binds exons at the 5' site. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Tango4 or the JBrowse view of Dmel\Tango4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tango4-RA

FBtr0073622

NM_132550

1620

482

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Tango4-PA

FBpp0073459

52.7

482

9.83

NP_572778

AAF48133

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tango4 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (9 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2011-06-28 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

Golgi organization

inferred from mutant phenotype

(Bard et al., 2006)

protein secretion

inferred from mutant phenotype

(Bard et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

mRNA splicing, via spliceosome

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

inferred from biological aspect of ancestor with PANTHER:PTN000460428

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

cytosol

inferred from direct assay

(Bard et al., 2006)

precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Prp19 complex

inferred from direct assay

(Guilgur et al., 2014)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

catalytic step 2 spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000460428

(assigned by GO_Central )

(Gaudet et al., 2011)

Cul4-RING E3 ubiquitin ligase complex

inferred from biological aspect of ancestor with PANTHER:PTN000460428

(assigned by GO_Central )

(Gaudet et al., 2011)

colocalizes_with DNA replication factor A complex

inferred from biological aspect of ancestor with PANTHER:PTN000460428

(assigned by GO_Central )

(Gaudet et al., 2011)

Prp19 complex

inferred from biological aspect of ancestor with PANTHER:PTN000460428

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

cytosol

inferred from direct assay

(Bard et al., 2006)

precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Prp19 complex

inferred from direct assay

(Guilgur et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Tango4 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tango4

View exonic expression by tissue for Dmel\Tango4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030365

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0030365

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1796-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1796

Flygut - An atlas of the Drosophila adult midgut

FBgn0030365

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tango4

Transgenic constructs containing regulatory region of Tango4

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tango4^GD8618

viable, with Scer\GAL4^Mef2.PR

Tango4^GD8618

viable, with Scer\GAL4^pnr-MD237

Tango4^GD8618

viable, with Scer\GAL4^tin.CΔ4

Tango4^GD8618

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PLRG1

14 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Plrg1

13 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Plrg1

13 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\plrg1

8 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\plrg1

12 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\plrg-1

14 of 15

Yes

Cele\tag-138

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT3G16650

9 of 9

Yes

Atha\PRL1

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\PRP46

14 of 15

Yes

Scer\TUP1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\prp5

12 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919072W )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Tango4

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14743

Drosophila persimilis

Dper\GL20273

Drosophila willistoni

Dwil\GK10085

Drosophila virilis

Dvir\GJ18540

Drosophila mojavensis

Dmoj\GI21768

Drosophila grimshawi

Dgri\GH24167

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150423 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17017981

Glossina morsitans

Tsetse fly

Gmor\GMOY006161

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10250

Mayetiola destructor

Hessian fly

Mdes\Mdes004389

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010588

Anopheles darlingi

American malaria mosquito

Adar\ADAC005989

Anopheles gambiae

Malaria mosquito

Agam\AGAP005631

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004561

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03U6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010974

Danaus plexippus

Monarch butterfly

Dple\DPOGS215570-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL010065

Apis florea

Little honeybee

Aflo\3084948244

Apis mellifera

Western honey bee

Amel\GB44597

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13776

Bombus terrestris

Buff-tailed bumblebee

Bter\15201883

Linepithema humile

Argentine ant

Lhum\LH24730

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0001f9

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006717

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07073

Tribolium castaneum

Red flour beetle

Tcas\TC001100

Pediculus humanus

Human body louse

Phum\PHUM407120

Rhodnius prolixus

Kissing bug

Rpro\RPRC007030

Cimex lectularius

Bed bug

Clec\CLEC003227

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005470

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02565

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03RP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004684

Strigamia maritima

European centipede

Smar\SMAR011543

Ixodes scapularis

Black-legged tick

Isca\ISCW018505

Stegodyphus mimosarum

African social velvet spider

Smim\KFM76417.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur28g01870

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_303553

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G08RE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Plrg1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009307

Gallus gallus

Domestic chicken

Ggal\PLRG1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PLRG1; pleiotropic regulator 1

14 of 15

605961

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tango4 - BCAS2

experimental knowledge based

(Guruharsha et al., 2011)

Tango4 - Bx42

experimental knowledge based

(Guruharsha et al., 2011)

Tango4 - CG14641

experimental knowledge based

(Guruharsha et al., 2011)

Tango4 - CG6015

experimental knowledge based

(Guruharsha et al., 2011)

Tango4 - Cdc5

experimental knowledge based

(Guruharsha et al., 2011)

Tango4 - Prp19

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Tango4 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Tango4 - fand

anti tag coimmunoprecipitation, peptide massfingerprinting

(Guilgur et al., 2014)

Tango4 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58573

DroID - A comprehensive database of gene and protein interactions.

FBgn0030365

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32168

MIST (protein-protein) - An integrated Molecular Interaction Database

Tango4

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Spliceosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

mRNA Splicing - Major Pathway

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-38

Cytogenetic map

11A1-11A1

Sequence location

X:12,021,068..12,022,746 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11A1-11A1

Limits computationally determined from genome sequence between P{EP}EP1451&P{EP}EP1629 and P{EP}CG11138^EP1313

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-38

(FlyBase, 2016)

1-37.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

VDRC

v101441

P{KK109170}VIE-260B

v44862

w¹¹¹⁸; P{GD8618}v44862/CyO

v318209

PBac{fTRG00721.sfGFP-TVPTBF}VK00033

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (63)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD24662

Other clones

DPiM_LD24662

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030365

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030365

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32168

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Tango4 CG1796

(Bard et al., 2006)

Source for database merge of

Additional comments

Other Comments

dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

(Bard et al., 2006)

dsRNA directed against Tango4 inhibits protein secretion in S2 cells and results in fusion of Golgi membranes with the endoplasmic reticulum in more than 50% of the treated cells.

(Bard et al., 2006)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32168

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VYQ9

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030365

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030365

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1796

Flygut - An atlas of the Drosophila adult midgut

FBgn0030365

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32168

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1796

modMine - A data warehouse for the modENCODE project

FBgn0030365

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VYQ9

Linkouts

BioGRID - A database of protein and genetic interactions.

58573

DPiM - Drosophila Protein interaction map

FBgn0030365

DroID - A comprehensive database of gene and protein interactions.

FBgn0030365

DRSC - Results frm RNAi screens

FBgn0030365

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0030365

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1796-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030365

FlyMine - An integrated database for Drosophila genomics

FBgn0030365

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32168

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Spliceosome

MIST (protein-protein) - An integrated Molecular Interaction Database

Tango4

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

mRNA Splicing - Major Pathway

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG1796

(Aradska et al., 2015, Hosono et al., 2015, Guilgur et al., 2014, Herold et al., 2009, Keleman et al., 2009.8.5, Stuart et al., 2007, Bard et al., 2006)

TANGO4

(Bard et al., 2006)

Tango4

(Sarov et al., 2016, Guilgur et al., 2014, Neumüller et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Herold et al., 2009)

tango4

(Tiwari et al., 2015)

Name Synonyms

Transport and Golgi organization 4

(Bard et al., 2006)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (32)

Report Sections

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