Gene Dmel\CG12096

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General Information

Symbol

Dmel\CG12096

Species

D. melanogaster

Name

Annotation symbol

CG12096

Feature type

protein_coding_gene

FlyBase ID

FBgn0030457

Gene Model Status

Current

Stock availability

4 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

11E1-11E1

Sequence location

X:13,053,637..13,056,887 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG12096 (FBgn0030457). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: protein binding involved in protein folding. There is experimental evidence that it is involved in the biological process: response to DNA damage stimulus. 7 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: 26S proteasome non-ATPase regulatory subunit 5; Armadillo-type fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1632182_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG12096. Gene sequence location is X:13053637..13056887.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E1-11E1

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG12096 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12096-RA

FBtr0073727

NM_132634

2040

506

CG12096-RB

FBtr0340298

1830

506

CG12096-RC

FBtr0340299

2985

506

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12096-PA

FBpp0073558

56.7

506

6.25

NP_572862

AAF48236

CG12096-PB

FBpp0309259

56.7

506

6.25

CG12096-PC

FBpp0309260

56.7

506

6.25

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Armadillo-type fold (IPR016024)

26S proteasome non-ATPase regulatory subunit 5 (IPR019538)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		158.55
Larval Midgut		69.1
Larval Hindgut		97.3
Larval Malpighian Tubules		133.8
Larval Fat Body		109.5
Larval Salivary Gland		186.1
Larval Trachea		102.7
Larval Carcass		104.125
Adult Head		82.8
Adult Eye		87.825
Adult Brain		103.9
Adult Thoracic-Abdominal Ganglion		117
Adult Crop		165
Adult Midgut		90.4
Adult Hindgut		88.9
Adult Malpighian Tubules		70.1
Adult Fat Body		128
Adult Salivary Gland		171.7
Adult Heart		146.45
Adult VirginFemale Spermatheca		63.3
Adult InseminatedFemale Spermatheca		67
Adult Ovary		146.8
Adult Testis		224.3
Adult Male Accessory Gland		154.6
Adult Carcass		101
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	82.8
NA	Eye	87.825
NA	Brain	103.9
158.55	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	117
NA	Crop	165
69.1	Midgut	90.4
97.3	Hindgut	88.9
133.8	Malpighian Tubules	70.1
109.5	Fat Body	128
186.1	Salivary Gland	171.7
NA	Heart	146.45
102.7	Trachea	NA
NA	VirginFemale Spermatheca	63.3
NA	InseminatedFemale Spermatheca	67
NA	Ovary	146.8
NA	Testis	224.3
NA	Male Accessory Gland	154.6
104.125	Carcass	101

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		11
central nervous system, larvae L3		5
central nervous system, pupae P8		3
head, virgin 1-day female		2
head, virgin 4-day female		1
head, virgin 20-day female		3
head, mated 1-day female		1
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		3
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		2
digestive system, 20-day adult		2
fat body, larvae L3 wandering		1
fat body, white prepupae		3
fat body, pupae P8		0
carcass, larvae L3 wandering		4
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		7
testis, mated 4-day male		11
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		(30)
white prepupae new		(36)
white prepupae 12hr		(35)
white prepupae 24hr		(39)
pupae 2d postWPP		(44)
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		(36)
white prepupae 12hr		(35)
white prepupae 24hr		(39)
pupae 2d postWPP		(44)
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		2
embryo 08-10hr		2
embryo 10-12hr		3
embryo 12-14hr		6
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		4
embryo 20-22hr		4
embryo 22-24hr		4
larva L1		5
larva L2		8
larva L3 12hr old		6
larva L3 puffstage 1-2		11
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		30
white prepupae new		36
white prepupae 12hr		35
white prepupae 24hr		39
pupae 2d postWPP		44
pupae 3d postWPP		22
pupae 4d postWPP		15
adult male 01day		10
adult male 05day		11
adult male 30day		9
adult female 01day		7
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		7
Schneider line 2 Sg4		9
embryonic 1182-4H		6
embryonic GM2		7
embryonic Kc167		6
embryonic S1		9
embryonic S3		9
leg disc CME L1		6
wing disc CME-W2		11
wing disc ML-DmD8		6
wing disc ML-DmD9		11
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		6
wing disc ML-DmD32		6
haltere disc ML-DmD17-c3		7
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		8
mixed discs ML-DmD4-c1		7
CNS ML-DmBG1-c1		8
CNS ML-DmBG2-c2		10
tumorous blood cells mbn2		7
ovary fGS/OSS		5
ovary OSC		7
ovary OSS		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		4
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		4
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		7
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		6
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD46817

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030457

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12096-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Sterility

Allele

fertile

CG12096^f05782

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of CG12096	Mutagen	Stocks	Known lesion
CG12096^f05782	piggyBac transposase	2	--
CG12096^EY23373	P-element activity	1	--
CG12096¹	P-element activity	0	Yes
CG12096^GG01131		0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12096	Mutagen	Stocks	Known lesion
CG12096^KK109345	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG12096^GD7478	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
CG12096^NIG.12096R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Partially disrupted in

Df(1)Bap60¹

(Moeller et al., 2005)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7478}

P{KK109345}

P{NIG.12096R}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}CG12096^f05782

insertion of mobile activating element

P{EPgy2}CG12096^EY23373

P{Mae-UAS.6.11}CG12096^GG01131

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

response to DNA damage stimulus

inferred from mutant phenotype

(Ravi et al., 2009)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function

CV term

Evidence

References

protein binding involved in protein folding

inferred from electronic annotation with InterPro:IPR019538

(FlyBase Curators et al., 2004-)

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12096 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

58654

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0030457

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32269

Orthologs

OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6S4Q1W

Dipteran inclusive ortholog search

EOG6V43H9

Insect inclusive ortholog search

EOG6QVB7H

Arthropod inclusive ortholog search

EOG6JDFP9

Metazoa inclusive ortholog search

EOG65QW6T

Orthologs in Drosophila Species (EOG6S4Q1W)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23967

Drosophila persimilis

Dper\GL15875

Drosophila willistoni

Dwil\GK16123

Drosophila virilis

Dvir\GJ16670

Drosophila mojavensis

Dmoj\GI16181

Drosophila grimshawi

Dgri\GH24846

Orthologs in non-Drosophila Dipterans (EOG6V43H9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011059

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013095

Anopheles gambiae

Malaria mosquito

Agam\AGAP001490

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004472

Orthologs in non-Dipteran Insects (EOG6QVB7H)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB49118

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005336

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH16127

Atta cephalotes

Leafcutter ant

Acep\ACEP13229

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17961

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL21410

Linepithema humile

Argentine ant

Lhum\LH23905

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB17144

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005559

Bombyx mori

Silkmoth

Bmor\BGIBMGA012532

Pediculus humanus

Human body louse

Phum\PHUM450860

Tribolium castaneum

Red flour beetle

Tcas\TC011419

Orthologs in non-Insect Arthropods (EOG6JDFP9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\psbC

Ixodes scapularis

Deer tick

Isca\ISCW015677

Orthologs in non-Arthropod Metazoa (EOG65QW6T)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\F35G12.12

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_012349gn

Danio rerio

Zebrafish

Drer\zgc:110116

Xenopus tropicalis

Western clawed frog

Xtro\psmd5

Gallus gallus

Domestic chicken

Ggal\PSMD5

Mus musculus

House mouse

Mmus\Psmd5

Rattus norvegicus

Norway rat

Rnor\Psmd5

Homo sapiens

Human

Hsap\PSMD5

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\PSMD5

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23967

Drosophila persimilis

Dper\GL15875

Drosophila willistoni

Dwil\GK16123

Drosophila virilis

Dvir\GJ16670

Drosophila mojavensis

Dmoj\GI16181

Drosophila grimshawi

Dgri\GH24846

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Bloomington

18906

w¹¹¹⁸ PBac{WH}CG12096^f05782

22627

y¹ w^67c23 P{EPgy2}CG12096^EY23373

Harvard

PBac{WH}CG12096^f05782

VDRC

v104492

P{KK109345}VIE-260B

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 27 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

FI18823

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0030457

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32269

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_132634

RefSeq (Proteins)

•

NP_572862

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

32269

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Armadillo-type fold (IPR016024)

26S proteasome non-ATPase regulatory subunit 5 (IPR019538)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD46817

Linkouts

BioGRID - A database of protein and genetic interactions

•

58654

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0030457

DRSC - Results from RNAi screens.

•

FBgn0030457

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030457

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG12096-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0030457

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32269

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32269

modMine - Data generated by the modENCODE project.

•

FBgn0030457

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

12096

(Ravi et al., 2009)

CG12096

(Keleman et al., 2009.8.5, Moeller et al., 2005, Japanese National Institute of Genetics, 2012.5.21, Gurudatta et al., 2012)

S5b/PSMD5

(Shim et al., 2012)

Name Synonym

Secondary FlyBase IDs

References ( 20 )

Research paper

Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132: The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796]
Shim et al., 2012, Cell Rep. 2(3): 603--615: Role of S5b/PSMD5 in Proteasome Inhibition Caused by TNF-α/NFκB in Higher Eukaryotes. [FBrf0219534]
Ravi et al., 2009, PLoS Genet. 5(6): e1000527: A network of conserved damage survival pathways revealed by a genomic RNAi screen. [FBrf0208272]
Moeller et al., 2005, J. Mol. Biol. 352(2): 329--337: Drosophila BAP60 is an essential component of the Brahma complex, required for gene activation and repression. [FBrf0187959]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Dietzl et al., 2007, Nature 448(7150):: Supplementary Table 1. [FBrf0202579]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Bellen, 2006.8.3, MBBatch686_2.xml: MBBatch686_2.xml [FBrf0191805]
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file): Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.: Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]
Oliver, 2001.8.16, Genome Res.:: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]

FlyBase analysis

FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]

Stock list

Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.: RNAi stocks available from NIG-FLY. [FBrf0218457]

Website

Fisher et al., 2012, BDGP insitu homepage: BDGP insitu homepage [FBrf0219073]