• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Ndc80

General Information
Symbol	Dmel\Ndc80	Species	D. melanogaster
Name	Ndc80	Annotation symbol	CG9938
Feature type	protein_coding_gene	FlyBase ID	FBgn0030500
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	Hec1, dmNdc80
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	12A8-12A8	Sequence location	X:13,503,704..13,506,095 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ndc80 is referred to in FlyBase by the symbol Dmel\Ndc80 (CG9938, FBgn0030500). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic metaphase plate congression; chromosome segregation; mitotic spindle organization; neurogenesis. 14 alleles are reported. The phenotypes of these alleles are annotated with: metaphase; trichogen cell; condensed nuclear chromosome; spindle; mesothoracic tergum; nucleus. It has one annotated transcript and one annotated polypeptide. Protein features are: Kinetochore protein Ndc80; Outer dense fibre protein 2-related. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1630409_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Ndc80. Gene sequence location is X:13503704..13506095.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 12A8-12A8   Limits computationally determined from genome sequence between P{EP}CG1640EP1626 and P{EP}NFATEP1353&P{EP}NFATEP1508
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ndc80 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ndc80-RA FBtr0073850  NM_132674   2200   675
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ndc80-PA   FBpp0073681   77.9   675   6.89     NP_572902   AAF48292
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinetochore protein Ndc80 (IPR005550) Outer dense fibre protein 2-related (IPR026099)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA978865   AE014298 AAF48292   AW943159   AY061402 AAL28950   BF504700   BI364832   CO303115   CO303622   CO318091   EC067144   EC225997   EC225998   EC227123
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VYB1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   cell   • kinetochore (Schittenhelm et al., 2007) Comment:Localization observed in mitotic cells at embryonic cycle 14 mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	Subcellular localization to kinetochores of embryonic mitotic cells assayed using the transgenic allele Ndc80T:Avic\GFP-EGFP. (Schittenhelm et al., 2007)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007) colocalizes_with kinetochore inferred from direct assay (Goshima et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut    17.9 Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body    19.4 Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass    20.95 Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion    11.8 Adult Crop    14.9 Adult Midgut    11.6 Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland    13.6 Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass    13.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    5.4   NA  Eye    4.2   NA  Brain    8.9   152.45  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    no informative data   17.9  Hindgut    9.2   23  Malpighian Tubules    8.4   no informative data  Fat Body    5.4   65.6  Salivary Gland    no informative data   NA  Heart    9.275   37.675  Trachea    NA   NA  VirginFemale Spermatheca    9.9   NA  InseminatedFemale Spermatheca    6.1   NA  Ovary    354   NA  Testis    37.8   NA  Male Accessory Gland    6.5   no informative data  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    4 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    25 ovary, mated 4-day female    22 testis, mated 4-day male    8 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (36) embryo 02-04hr    17 embryo 04-06hr  (43) embryo 06-08hr  (34) embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (36) embryo 02-04hr    17 embryo 04-06hr  (43) embryo 06-08hr  (34) embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    36 embryo 02-04hr    17 embryo 04-06hr    43 embryo 06-08hr    34 embryo 08-10hr    20 embryo 10-12hr    8 embryo 12-14hr    3 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    2 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    4 white prepupae 12hr    8 white prepupae 24hr    6 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    8 adult female 05day    17 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS  (27) ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS  27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    12 Schneider line 2 Sg4    18 embryonic 1182-4H    13 embryonic GM2    12 embryonic Kc167    12 embryonic S1    15 embryonic S3    10 leg disc CME L1    15 wing disc CME-W2    10 wing disc ML-DmD8    13 wing disc ML-DmD9    14 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    24 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    11 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    14 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    3 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • LD33040 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030500 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9938-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Ndc80GD14729 viable, with Scer\GAL4pnr-MD237 Ndc80GD14729 Other Phenotypes Allele mitotic cell cycle defective Ndc80dsRNA.cPa neuroanatomy defective | larval stage Ndc80GD14729 visible, with Scer\GAL4pnr-MD237 Ndc80GD14729 Phenotype manifest in Allele condensed nuclear chromosome Ndc80dsRNA.cPa embryonic/larval neuroblast | larval stage Ndc80GD14729 ganglion mother cell | larval stage Ndc80GD14729 mesothoracic tergum, with Scer\GAL4pnr-MD237 Ndc80GD14729 metaphase Ndc80dsRNA.cPa nucleus Ndc80dsRNA.cPa spindle Ndc80dsRNA.cPa trichogen cell, with Scer\GAL4pnr-MD237 Ndc80GD14729
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Ndc80 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ndc80 Class Mutagen Stocks Known lesion Ndc80GD14729 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ndc80GL00625 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ndc80HMS01704 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ndc80Act5C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80Act5C.T:Saur\A in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80Act5C.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80dsRNA.cPa in vitro construct - RNAi 0 Yes Ndc80MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80MtnA.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80NIG.9938R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ndc80Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Ndc80T:Disc\RFP-mRFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6246 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14729} NA P{NIG.9938R} NA P{TRiP.GL00625} NA P{TRiP.HMS01704} NA characterization construct P{gEGFP-Ndc80} NA P{gmRFP1-Ndc80} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome segregation inferred from mutant phenotype (Przewloka et al., 2007) mitotic metaphase plate congression inferred from mutant phenotype (Przewloka et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007) colocalizes_with kinetochore inferred from direct assay (Goshima et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ndc80 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58693 DroID - A comprehensive database of gene and protein interactions. • FBgn0030500 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32316
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG68D1DV Dipteran inclusive ortholog search EOG6VT65C Insect inclusive ortholog search EOG6R22PW Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG68D1DV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Ndc80     Drosophila simulans   Dsim\GD24521 Y   Drosophila sechellia   Dsec\GM17605 Y   Drosophila erecta   Dere\GG19498 Y   Drosophila yakuba   Dyak\GE16152 Y   Drosophila ananassae   Dana\GF22373 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA22139 Y   Drosophila persimilis   Dper\GL20321 Y   Drosophila willistoni   Dwil\GK19995 Y   Drosophila virilis   Dvir\GJ18636 Y   Drosophila mojavensis   Dmoj\GI15779 Y   Drosophila grimshawi   Dgri\GH12244 Y
Orthologs in non-Drosophila Dipterans (EOG6VT65C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011220   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004073   Anopheles gambiae Malaria mosquito  Agam\AGAP006661   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003946
Orthologs in non-Dipteran Insects (EOG6R22PW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Solenopsis invicta Red fire ant  Sinv\SINV16385
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24521 Drosophila sechellia Dsec\GM17605 Drosophila erecta Dere\GG19498 Drosophila yakuba Dyak\GE16152 Drosophila ananassae Dana\GF22373 Drosophila pseudoobscura pseudoobscura Dpse\GA22139 Drosophila persimilis Dper\GL20321 Drosophila willistoni Dwil\GK19995 Drosophila virilis Dvir\GJ18636 Drosophila mojavensis Dmoj\GI15779 Drosophila grimshawi Dgri\GH12244
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	37482 y1 sc* v1; P{TRiP.GL00625}attP40 38260 y1 sc* v1; P{TRiP.HMS01704}attP40
VDRC	v29337 w1118; P{GD14729}v29337
Genomic Clones ( 1 )
	BACR30C16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 9 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD33040
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06330 RE50022
Other clones	120292 165867 177597 EK240040 EK242240 EK270282
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0030500 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32316
Antibody Information
	polyclonal (Venkei et al., 2011)
Other Information
Discoverer
Etymology
Identification
	On the basis of sequence similarity to the human gene NDC80 (OMIM:607272). (Przewloka et al., 2007)
Relationship to Other Genes
Source for database identity of	Source for identity of: Ndc80 CG9938 (Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and a spindle that is aberrantly long when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132674 RefSeq (Proteins) • NP_572902 DDBJ / EMBL / GenBank • AA978865 AAF48292 AAL28950 AW943159 AY061402 Entrez Gene - A searchable database of RefSeq genes. • 32316 UniProtKB/TrEMBL • Q9VYB1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinetochore protein Ndc80 (IPR005550) Outer dense fibre protein 2-related (IPR026099) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD33040
Linkouts
	BioGRID - A database of protein and genetic interactions • 58693 DroID - A comprehensive database of gene and protein interactions. • FBgn0030500 DRSC - Results from RNAi screens. • FBgn0030500 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030500 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9938-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030500 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32316 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32316 modMine - Data generated by the modENCODE project. • FBgn0030500
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG9938 (Goshima et al., 2007, Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007, Ni et al., 2011.7.19, Cammarato et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2010.12.1) dmNdc80 (Tavares et al., 2007, Przewloka et al., 2007) dNdc80 (Meireles et al., 2011) Hec1 (Somma et al., 2008, Williams et al., 2007) hec1 (Bucciarelli et al., 2009) Ndc80 (Schittenhelm et al., 2009, Cane et al., 2013, Schittenhelm et al., 2007, Goshima et al., 2007, Somma et al., 2008, Maresca and Salmon, 2009, Williams et al., 2007, Lince-Faria et al., 2009, White and Erickson, 2009, Furuyama and Henikoff, 2009, Bucciarelli et al., 2009, Meraldi et al., 2006, Schittenhelm et al., 2010, Venkei et al., 2011, Przewloka et al., 2011, Neumüller et al., 2011, Meireles et al., 2011, Venkei et al., 2012) NDC80 (Olszak et al., 2011)
Name Synonym	CG9938   Hec1 (Somma et al., 2008) Ndc80 (Somma et al., 2008)
Secondary FlyBase IDs
References ( 40 )
Generate a list of	All references relating to this gene ( 40 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Cane et al., 2013, J. Cell Biol. 200(2): 203--218 Elevated polar ejection forces stabilize kinetochore-microtubule attachments. [FBrf0220555] Moutinho-Santos et al., 2012, J. Cell Sci. 125(3): 576--583 POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments. [FBrf0217659] Venkei et al., 2012, Open Biol. 2(2): 110032 Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. [FBrf0218508] Meireles et al., 2011, PLoS ONE 6(9): e24174 Kebab: Kinetochore and EB1 Associated Basic Protein That Dynamically Changes Its Localisation during Drosophila Mitosis. [FBrf0215262] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Olszak et al., 2011, Nat. Cell Biol. 13(7): 799--808 Heterochromatin boundaries are hotspots for de novo kinetochore formation. [FBrf0214065] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Venkei et al., 2011, Genetics 187(1): 131--140 Drosophila mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint. [FBrf0212773] Show all research papers ...