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Gene Dmel\Ndc80

General Information
Symbol	Dmel\Ndc80	Species	D. melanogaster
Name	Ndc80	Annotation symbol	CG9938
Feature type	protein_coding_gene	FlyBase ID	FBgn0030500
Gene Model Status	Current	Stock availability	1 publicly available
Also Known As	Hec1, dmNdc80
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	12A8-12A8	Sequence location	X:13,503,698..13,506,009 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ndc80 is referred to in FlyBase by the symbol Dmel\Ndc80 (CG9938, FBgn0030500). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic metaphase plate congression; chromosome segregation; mitotic spindle organization; neurogenesis. 11 alleles are reported. The phenotypes of these alleles are annotated with: metaphase; trichogen cell; condensed nuclear chromosome; spindle; mesothoracic tergum; nucleus. It has one annotated transcript and one annotated polypeptide. Protein features are: Kinetochore protein Ndc80. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1630409_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Ndc80. Gene sequence location is X:13503698..13506009.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Meireles et al., 2011, PLoS ONE 6(9): e24174 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Sequence features BKN23993 HFA20221
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 12A8-12A8   Limits computationally determined from genome sequence between P{EP}CG1640EP1626 and P{EP}NFATEP1353&P{EP}NFATEP1508
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ndc80 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ndc80-RA FBtr0073850  NM_132674   2120   675
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ndc80-PA   FBpp0073681   77.9   675   6.89     NP_572902   AAF48292
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinetochore protein Ndc80 (IPR005550)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA978865   AE014298 AAF48292   AW943159   AY061402 AAL28950   BF504700   BI364832   CO303115   CO303622   CO318091   EC067144   EC225997   EC225998   EC227123
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VYB1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
epitope tag Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 5   cell   • kinetochore (Schittenhelm et al., 2007) Comment:Localization observed in mitotic cells at embryonic cycle 14 mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	Subcellular localization to kinetochores of embryonic mitotic cells assayed using the transgenic allele Ndc80T:Avic\GFP-EGFP. (Schittenhelm et al., 2007)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0030500    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0030500 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0030500 expression level Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2822) embryo 02-04hr  (1328) embryo 04-06hr  (3358) embryo 06-08hr  (2662) embryo 08-10hr  (1538) embryo 10-12hr  (639) embryo 12-14hr  (267) embryo 14-16hr  (137) embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2  107 larva L3 12hr old  (158) larva L3 puffstage 1-2  (248) larva L3 puffstage 3-6  (335) larva L3 puffstage 7-9  (375) white prepupae new  (319) white prepupae 12hr  (589) white prepupae 24hr  (464) pupae 2d postWPP  (153) pupae 3d postWPP    85 pupae 4d postWPP  (136) adult male 01day  (161) adult male 05day  (218) adult male 30day  (219) adult female 01day  (608) adult female 05day  (1309) adult female 30day  (1486) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2822) embryo 02-04hr    1328 embryo 04-06hr  (3358) embryo 06-08hr  (2662) embryo 08-10hr  (1538) embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day  1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0030500 expression level Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2822) embryo 02-04hr  (1328) embryo 04-06hr  (3358) embryo 06-08hr  (2662) embryo 08-10hr  (1538) embryo 10-12hr  (639) embryo 12-14hr  (267) embryo 14-16hr  137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2  107 larva L3 12hr old  (158) larva L3 puffstage 1-2  (248) larva L3 puffstage 3-6  (335) larva L3 puffstage 7-9  (375) white prepupae new  (319) white prepupae 12hr  (589) white prepupae 24hr  (464) pupae 2d postWPP  (153) pupae 3d postWPP    85 pupae 4d postWPP  136 adult male 01day  (161) adult male 05day  (218) adult male 30day  (219) adult female 01day  (608) adult female 05day  (1309) adult female 30day  (1486) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2822) embryo 02-04hr    1328 embryo 04-06hr  (3358) embryo 06-08hr  (2662) embryo 08-10hr  1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day  1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2822 embryo 02-04hr    1328 embryo 04-06hr    3358 embryo 06-08hr    2662 embryo 08-10hr    1538 embryo 10-12hr    639 embryo 12-14hr    267 embryo 14-16hr    137 embryo 16-18hr    85 embryo 18-20hr    95 embryo 20-22hr    48 embryo 22-24hr    67 larva L1    84 larva L2    107 larva L3 12hr old    158 larva L3 puffstage 1-2    248 larva L3 puffstage 3-6    335 larva L3 puffstage 7-9    375 white prepupae new    319 white prepupae 12hr    589 white prepupae 24hr    464 pupae 2d postWPP    153 pupae 3d postWPP    85 pupae 4d postWPP    136 adult male 01day    161 adult male 05day    218 adult male 30day    219 adult female 01day    608 adult female 05day    1309 adult female 30day    1486 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0030500    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0030500 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0030500 expression level Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0030500 expression level Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    152.45 Larval Midgut no informative data Larval Hindgut    17.9 Larval Malpighian Tubules    23 Larval Fat Body no informative data Larval Salivary Gland    65.6 Larval Trachea    37.675 Larval Carcass no informative data Adult Head    5.4 Adult Eye    4.2 Adult Brain    8.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    9.2 Adult Malpighian Tubules    8.4 Adult Fat Body    5.4 Adult Salivary Gland no informative data Adult Heart    9.275 Adult VirginFemale Spermatheca    9.9 Adult InseminatedFemale Spermatheca    6.1 Adult Ovary    354 Adult Testis    37.8 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    5.4   NA  Eye    4.2   NA  Brain    8.9   152.45  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    no informative data   17.9  Hindgut    9.2   23  Malpighian Tubules    8.4   no informative data  Fat Body    5.4   65.6  Salivary Gland    no informative data   NA  Heart    9.275   37.675  Trachea    NA   NA  VirginFemale Spermatheca    9.9   NA  InseminatedFemale Spermatheca    6.1   NA  Ovary    354   NA  Testis    37.8   NA  Male Accessory Gland    6.5   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030500 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9938-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 Ndc80GD14729 viable, with Scer\GAL4pnr-MD237 Ndc80GD14729 Other Phenotypes Allele mitotic cell cycle defective Ndc80dsRNA.cPa neuroanatomy defective | larval stage, with Scer\GAL41407 Ndc80GD14729 visible, with Scer\GAL4pnr-MD237 Ndc80GD14729 Phenotype manifest in Allele condensed nuclear chromosome Ndc80dsRNA.cPa embryonic/larval neuroblast | larval stage, with Scer\GAL41407 Ndc80GD14729 ganglion mother cell | larval stage, with Scer\GAL41407 Ndc80GD14729 mesothoracic tergum, with Scer\GAL4pnr-MD237 Ndc80GD14729 metaphase Ndc80dsRNA.cPa nucleus Ndc80dsRNA.cPa spindle Ndc80dsRNA.cPa trichogen cell, with Scer\GAL4pnr-MD237 Ndc80GD14729
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Ndc80 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ndc80 Class Mutagen Stocks Known lesion Ndc80GD14729 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ndc80Act5C.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80Act5C.T:Saur\A in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80Act5C.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80dsRNA.cPa in vitro construct - RNAi 0 Yes Ndc80MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80MtnA.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ndc80T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Ndc80T:Disc\RFP-mRFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6246 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14729} NA characterization construct P{gEGFP-Ndc80} NA P{gmRFP1-Ndc80} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromosome segregation inferred from mutant phenotype (Przewloka et al., 2007) mitotic metaphase plate congression inferred from mutant phenotype (Przewloka et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
kinetochore inferred from direct assay (Przewloka et al., 2007, Schittenhelm et al., 2007) colocalizes_with kinetochore inferred from direct assay (Goshima et al., 2007) Ndc80 complex inferred from direct assay (Schittenhelm et al., 2007)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ndc80 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58693 DroID - A comprehensive database of gene and protein interactions. • FBgn0030500 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32316
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22373 Dere\GG19498 Dgri\GH12244 Dmoj\GI15779 Dper\GL20321 Dpse\GA22139 Dsec\GM17605 Dsim\GD24521 Dvir\GJ18636 Dwil\GK19995 Dyak\GE16152
Curated drosophilid orthologs	Dpse\GA22139
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011220-PA Aedes aegypti (mosquito) AGAP006661-PA Anopheles gambiae (mosquito) XP_623608.1 Apis mellifera (honey bee) XP_001946571.1 Acyrthosiphon pisum (pea aphid) 124179 Branchiostoma floridae (lancelet) BGIBMGA008679-PA Bombyx mori (silk worm) ENSBTAP00000031011 Bos taurus (bovine) CE06537 Caenorhabditis elegans (worm) ENSCAFP00000027183 Canis familiaris (dog) JA03508 Caenorhabditis japonica (nematode) cn05274 Cryptococcus neoformans (yeast-like fungus) CPIJ003946 Culex pipens (mosquito) ENSCPOP00000005266 Cavia porcellus (guinea pig) 141966 Chlamidomonas reinhardtii (green alga) RP13023 Caenorhabditis remanei (nematode) 227392 Capitella spl (polychaete worm) 314991 Daphnia pulex (daphnia) ENSECAP00000007056 Equus caballus (horse) ENSGALP00000023851 Gallus gallus (chicken) ENSP00000261597 Homo sapiens (human) ENSMODP00000026826 Monodelphis domestica (gray opossum) ENSMMUP00000024940 Macaca mulatta (rhesus monkey) ENSMUSP00000024851 Mus musculus (house mouse) ENSOANP00000017183 Ornithorynchus anatinus (platypus) PFF0785w Plasmodium falciparum (protozoal parasite) PHUM008705-PA Pediculus humanus (human body louse) 162337 Physcomitrella patens (moss) ENSPPYP00000010143 Pongo pygmaeus (orangutan) 73145 Phytophthora ramorum (sudden oak death pathogen) 129895 Phytophthora sojae (root rot pathogen) 751666 Populus trichocarpa (black cottonwood) ENSPTRP00000016733 Pan troglodytes (chimpanzee) PVX_113945 Plasmodium vivax (protozoal parasite) ENSRNOP00000018644 Rattus norvegicus (norway rat) SPBC11C11.03 Schizosaccharomyces pombe (fission yeast) XP_001197919.1 Strongylocentrotus purpuratus (purple sea urchin) 53609 Trichoplax adhaerens (metazoa) ENSTRUP00000016950 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV25930 LH12870 FBgn0233696 FBgn0221992 FBgn0205791 FBgn0195855 FBgn0172513 JGI_V11_314991 FBgn0082119 FBgn0157916 FBgn0138528 FBgn0119723 FBgn0111700 FBgn0099369 CPIJ003946 BGIBMGA008679 ACYPI004318 GB11171 AGAP006661 AAEL011220 AAEL004073 PB20326 PHUM338290 GLEAN_11249
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
VDRC	v29337 w1118; P{GD14729}v29337
Genomic Clones ( 1 )
	BACR30C16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 9 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD33040
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06330 RE50022
Other clones	120292 165867 177597 EK240040 EK242240 EK270282
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0030500 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32316
Antibody Information
	polyclonal (Venkei et al., 2011)
Other Information
Discoverer
Etymology
Identification
	On the basis of sequence similarity to the human gene NDC80 (OMIM:607272). (Przewloka et al., 2007)
Relationship to Other Genes
Source for database identity of	Source for identity of: Ndc80 CG9938 (Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and a spindle that is aberrantly long when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132674 RefSeq (Proteins) • NP_572902 DDBJ / EMBL / GenBank • AA978865 AAF48292 AAL28950 AW943159 AY061402 Entrez Gene - A searchable database of RefSeq genes. • 32316 UniProtKB/TrEMBL • Q9VYB1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinetochore protein Ndc80 (IPR005550)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58693 DroID - A comprehensive database of gene and protein interactions. • FBgn0030500 DRSC - Results from RNAi screens. • FBgn0030500 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030500 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9938-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030500 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32316 InParanoid A subset of ortholog calls from InParanoid. • AAEL011220-PA Aedes aegypti (mosquito) AGAP006661-PA Anopheles gambiae (mosquito) XP_623608.1 Apis mellifera (honey bee) XP_001946571.1 Acyrthosiphon pisum (pea aphid) 124179 Branchiostoma floridae (lancelet) BGIBMGA008679-PA Bombyx mori (silk worm) ENSBTAP00000031011 Bos taurus (bovine) CE06537 Caenorhabditis elegans (worm) ENSCAFP00000027183 Canis familiaris (dog) JA03508 Caenorhabditis japonica (nematode) cn05274 Cryptococcus neoformans (yeast-like fungus) CPIJ003946 Culex pipens (mosquito) ENSCPOP00000005266 Cavia porcellus (guinea pig) 141966 Chlamidomonas reinhardtii (green alga) RP13023 Caenorhabditis remanei (nematode) 227392 Capitella spl (polychaete worm) 314991 Daphnia pulex (daphnia) ENSECAP00000007056 Equus caballus (horse) ENSGALP00000023851 Gallus gallus (chicken) ENSP00000261597 Homo sapiens (human) ENSMODP00000026826 Monodelphis domestica (gray opossum) ENSMMUP00000024940 Macaca mulatta (rhesus monkey) ENSMUSP00000024851 Mus musculus (house mouse) ENSOANP00000017183 Ornithorynchus anatinus (platypus) PFF0785w Plasmodium falciparum (protozoal parasite) PHUM008705-PA Pediculus humanus (human body louse) 162337 Physcomitrella patens (moss) ENSPPYP00000010143 Pongo pygmaeus (orangutan) 73145 Phytophthora ramorum (sudden oak death pathogen) 129895 Phytophthora sojae (root rot pathogen) 751666 Populus trichocarpa (black cottonwood) ENSPTRP00000016733 Pan troglodytes (chimpanzee) PVX_113945 Plasmodium vivax (protozoal parasite) ENSRNOP00000018644 Rattus norvegicus (norway rat) SPBC11C11.03 Schizosaccharomyces pombe (fission yeast) XP_001197919.1 Strongylocentrotus purpuratus (purple sea urchin) 53609 Trichoplax adhaerens (metazoa) ENSTRUP00000016950 Takifugu rubripes (pufferfish Takifugu) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32316 modMine - Data generated by the modENCODE project. • FBgn0030500 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV25930 LH12870 FBgn0233696 FBgn0221992 FBgn0205791 FBgn0195855 FBgn0172513 JGI_V11_314991 FBgn0082119 FBgn0157916 FBgn0138528 FBgn0119723 FBgn0111700 FBgn0099369 CPIJ003946 BGIBMGA008679 ACYPI004318 GB11171 AGAP006661 AAEL011220 AAEL004073 PB20326 PHUM338290 GLEAN_11249
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG9938 (Goshima et al., 2007, Schittenhelm et al., 2007, Przewloka et al., 2007, Goshima et al., 2007, Cammarato et al., 2011) dmNdc80 (Tavares et al., 2007, Przewloka et al., 2007) dNdc80 (Meireles et al., 2011) Hec1 (Somma et al., 2008, Williams et al., 2007) hec1 (Bucciarelli et al., 2009) Ndc80 (Schittenhelm et al., 2009, Schittenhelm et al., 2007, Goshima et al., 2007, Somma et al., 2008, Maresca and Salmon, 2009, Williams et al., 2007, Lince-Faria et al., 2009, White and Erickson, 2009, Furuyama and Henikoff, 2009, Bucciarelli et al., 2009, Meraldi et al., 2006, Schittenhelm et al., 2010, Venkei et al., 2011, Przewloka et al., 2011, Neumüller et al., 2011, Meireles et al., 2011)
Name Synonym	CG9938   Hec1 (Somma et al., 2008) Ndc80 (Somma et al., 2008)
Secondary FlyBase IDs
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 19 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 2 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 6 )
	Meireles et al., 2011, PLoS ONE 6(9): e24174 Kebab: Kinetochore and EB1 Associated Basic Protein That Dynamically Changes Its Localisation during Drosophila Mitosis. [FBrf0215262] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Venkei et al., 2011, Genetics 187(1): 131--140 Drosophila mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint. [FBrf0212773] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Schittenhelm et al., 2010, J. Cell Sci. 123(21): 3768--3779 Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1. [FBrf0212150] Show all research papers ...