FlyBase Gene Report: Dmel\BthD

General Information

Symbol

Dmel\BthD

Species

D. melanogaster

Name

BthD selenoprotein

Annotation Symbol

CG11177

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030501

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Snapshot

BthD selenoprotein (BthD) encodes a protein involved in salivary gland development and lifespan determination. [Date last reviewed: 2019-09-19]

Other Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

SelH

Key Links

Genomic Location

Cytogenetic map

12A8-12A8

Sequence location

X:13,612,131..13,613,228 [+]

Recombination map

1-44

RefSeq locus

NC_004354 REGION:13612131..13613228

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from direct assay

(assigned by UniProt )

(Kwon et al., 2003)

involved_in embryo development ending in birth or egg hatching

inferred from direct assay

(assigned by UniProt )

(Castellano et al., 2001)

involved_in larval salivary gland morphogenesis

inferred from mutant phenotype

(Kwon et al., 2003)

involved_in salivary gland boundary specification

inferred from direct assay

(assigned by UniProt )

(Kwon et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cell redox homeostasis

traceable author statement

(assigned by UniProt )

(Castellano et al., 2001)

involved_in response to redox state

non-traceable author statement

(assigned by UniProt )

(Martin-Romero et al., 2001)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kwon et al., 2003)

located_in Golgi apparatus

inferred from direct assay

(Kwon et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN002132385

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Selenoprotein, Rdx-type

Summaries

Protein Function (UniProtKB)

May be involved in a redox-related process. Required for survival and specifically for salivary gland morphogenesis.

(UniProt, Q9VYB0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\BthD for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.39

gene_with_stop_codon_redefined_as_selenocysteine ; SO:0000710

Stop-codon suppression (UGA as Sec) postulated (FBrf0137271).

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

gene_with_stop_codon_redefined_as_selenocysteine

(Martin-Romero et al., 2001)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

BthD-RA

FBtr0073806

NM_132675

977

249

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

BthD-PA

FBpp0073637

27.8

249

10.44

NP_572903

AAF48293

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Selenoprotein, Rdx-type

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VYB0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\BthD using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

BthD_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | intense

Comment: assayed 0-15 hr AEL

(Kwon et al., 2003)

larval stage

organism | faint

(Kwon et al., 2003)

adult stage | female

organism | intense

(Kwon et al., 2003)

adult stage | male

organism | faint

(Kwon et al., 2003)

Additional Descriptive Data

BthD transcript is detected at high levels in embryos from 0-5 hours AEL, and in adult females. BthD is detected at moderate levels from 5-15 hours ALE, and at negligible levels during all three larval instars, and in adult males.

(Kwon et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

ovariole

(Kwon et al., 2003)

oocyte

(Kwon et al., 2003)

embryonic stage 1 -- 4

organism | intense | ubiquitous | uniform

Comment: maternal contribution

(Kwon et al., 2003)

embryonic stage 5 -- 10

organism | faint | ubiquitous

(Kwon et al., 2003)

embryonic stage 14 -- 16

salivary gland body primordium | intense

(Kwon et al., 2003)

Additional Descriptive Data

BthD protein is is detected in ovarioles at all oogenesis stages. It is localized to the cytoplasm of oocytes, and is not detected in follicle or nurse cells. Maternally contributed BthD protein is detected in embryos until cellularization, and is much reduced by embryonic stage 10. BthD protein is detected in the salivary glands at stage 14, accumulating through stage 16.

(Kwon et al., 2003)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kwon et al., 2003)

located_in Golgi apparatus

inferred from direct assay

(Kwon et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\BthD in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\BthD

View exonic expression by tissue for Dmel\BthD

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0030501

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11177-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11177

Flygut - An atlas of the Drosophila adult midgut

FBgn0030501

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of BthD

Transgenic constructs containing regulatory region of BthD

Deletions and Duplications ( 1 )

Disrupted in

Df(1)Exel6246

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of P-stage, with Scer\GAL4^da.G32

BthD^dsRNA.UAS

lethal - all die before end of P-stage, with Scer\GAL4^fkh.PH

BthD^dsRNA.UAS

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

BthD^GD6665

partially lethal - majority die, with Scer\GAL4^pnr-MD237

BthD^GD6665

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

BthD^GD6665

Other Phenotypes

Allele

decreased cell size | third instar larval stage, with Scer\GAL4^da.G32

BthD^dsRNA.UAS

decreased cell size | third instar larval stage, with Scer\GAL4^fkh.PH

BthD^dsRNA.UAS

some die during P-stage, with Scer\GAL4^da.G32

BthD^dsRNA.UAS

some die during P-stage, with Scer\GAL4^fkh.PH

BthD^dsRNA.UAS

some die during pupal stage, with Scer\GAL4^pnr-MD237

BthD^GD6665

visible, with Scer\GAL4^pnr-MD237

BthD^GD6665

Phenotype manifest in

Allele

embryonic/larval salivary gland, with Scer\GAL4^da.G32

BthD^dsRNA.UAS

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

BthD^dsRNA.UAS

mesothoracic tergum, with Scer\GAL4^pnr-MD237

BthD^GD6665

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SELENOH

7 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Selenoh

6 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Selenoh

3 of 13

Yes

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (1)

Drer\selenoh

3 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (2)

Atha\AT4G31360

3 of 9

Yes

Atha\AT2G24440

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190HMU )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA27918

Drosophila willistoni

Drosophila virilis

Drosophila grimshawi

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0OOD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_52874

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

3 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SELENOH; selenoprotein H

7 of 15

607914

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

BthD - Sbp2

electrophoretic mobility shift assay, autoradiography

(Takeuchi et al., 2009)

protein-protein

Physical Interaction

Assay

References

BthD - Arc1

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG1129

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG1218

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG1532

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG18787

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG18789

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG2540

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG4707

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG5746

experimental knowledge based

(Guruharsha et al., 2011)

BthD - CG7049

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Ccs

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Cpr11B

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Fkbp12

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Got1

experimental knowledge based

(Guruharsha et al., 2011)

BthD - RpS21

experimental knowledge based

(Guruharsha et al., 2011)

BthD - Wdr82

experimental knowledge based

(Guruharsha et al., 2011)

BthD - knrl

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58694

DroID - A comprehensive database of gene and protein interactions.

FBgn0030501

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32317

MIST (protein-protein) - An integrated Molecular Interaction Database

BthD

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-44

Cytogenetic map

12A8-12A8

Sequence location

X:13,612,131..13,613,228 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12A8-12A8

Limits computationally determined from genome sequence between P{EP}CG1640^EP1626 and P{EP}NFAT^EP1353&P{EP}NFAT^EP1508

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-44

(FlyBase, 2016)

1-43.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Bloomington

43267

y¹ sc^* v¹ sev²¹; P{TRiP.GLC01456}attP2

65094

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05991}attP40

VDRC

v45975

w¹¹¹⁸; P{GD6665}v45975/TM3

Genomic Clones (5)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030501

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030501

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32317

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kwon et al., 2003)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: BthD CG11177

(FlyBase, 1996-)

Source for database merge of

Additional comments

Source for identity of BthD CG11177 was sequence comparison ( date:010728 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 27 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32317

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information