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Gene Dmel\BthD

General Information
Symbol	Dmel\BthD	Species	D. melanogaster
Name	BthD selenoprotein	Annotation symbol	CG11177
Feature type	protein_coding_gene	FlyBase ID	FBgn0030501
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	dselH, SelH
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	12A8-12A8	Sequence location	X:13,506,147..13,507,261 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene BthD selenoprotein is referred to in FlyBase by the symbol Dmel\BthD (CG11177, FBgn0030501). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: selenium binding. There is experimental evidence that it is involved in the biological process: larval salivary gland morphogenesis. 5 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval salivary gland; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Selenoprotein, Rdx type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1631037_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of BthD. Gene sequence location is X:13506147..13507261.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 12A8-12A8   Limits computationally determined from genome sequence between P{EP}CG1640EP1626 and P{EP}NFATEP1353&P{EP}NFATEP1508
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\BthD for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_stop_codon_redefined_as_selenocysteine ; SO:0000710 Gene model reviewed during 5.39 Stop-codon suppression (UGA as Sec) postulated (FBrf0137271).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) BthD-RA FBtr0073806  NM_132675   994   249
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein BthD-PA   FBpp0073637   27.8   249   10.44     NP_572903   AAF48293
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Selenoprotein, Rdx type (IPR011893)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696757   AA735222   AA816435   AA978403   AE014298 AAF48293   AF396455 AAK72982   AI542675   AY119185 AAM51045   BI166564   BI215604   BI230115   BI352834   BI353941   BI353960   BI361815   BI361840   BI364184   BI365768   BI484586   BI635919   BP559433   BP559434   BT088836   BT100173   CK135867   CO153539   CO154411   CO293382   CO340648   EC263763   EC265478   EV575849   EV579995
UniProtKB/Swiss-Prot	Q9VYB0
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | intense   (Kwon et al., 2003) Comment:assayed 0-15 hr AEL larval stage   organism | faint   (Kwon et al., 2003) adult stage | female   organism | intense   (Kwon et al., 2003) adult stage | male   organism | faint   (Kwon et al., 2003)
Additional Descriptive Data
	BthD transcript is detected at high levels in embryos from 0-5 hours AEL, and in adult females. BthD is detected at moderate levels from 5-15 hours ALE, and at negligible levels during all three larval instars, and in adult males. (Kwon et al., 2003)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 4   organism | intense & ubiquitous & uniform   (Kwon et al., 2003) Comment:maternal contribution embryonic stage 5 -- 10   organism | faint & ubiquitous   (Kwon et al., 2003) embryonic stage 14 -- 16   salivary gland body primordium | intense   (Kwon et al., 2003) oogenesis   ovariole   (Kwon et al., 2003) oocyte   (Kwon et al., 2003)
Additional Descriptive Data
	BthD protein is is detected in ovarioles at all oogenesis stages. It is localized to the cytoplasm of oocytes, and is not detected in follicle or nurse cells. Maternally contributed BthD protein is detected in embryos until cellularization, and is much reduced by embryonic stage 10. BthD protein is detected in the salivary glands at stage 14, accumulating through stage 16. (Kwon et al., 2003)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Kwon et al., 2003) Golgi apparatus inferred from direct assay (Kwon et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    372.25 Larval Midgut    14.6 Larval Hindgut    18.8 Larval Malpighian Tubules    5.7 Larval Fat Body    16.1 Larval Salivary Gland    8.6 Larval Trachea    74 Larval Carcass    31.55 Adult Head    3.8 Adult Eye    2.925 Adult Brain    2.3 Adult Thoracic-Abdominal Ganglion    5.8 Adult Crop    6.9 Adult Midgut    10.4 Adult Hindgut    5.9 Adult Malpighian Tubules    5.3 Adult Fat Body    7.6 Adult Salivary Gland    8.9 Adult Heart    2.225 Adult VirginFemale Spermatheca    5.5 Adult InseminatedFemale Spermatheca    3 Adult Ovary    438.3 Adult Testis    31.4 Adult Male Accessory Gland    1.6 Adult Carcass    5.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.8   NA  Eye    2.925   NA  Brain    2.3   372.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    5.8   NA  Crop    6.9   14.6  Midgut    no informative data   18.8  Hindgut    5.9   5.7  Malpighian Tubules    5.3   16.1  Fat Body    7.6   8.6  Salivary Gland    8.9   NA  Heart    2.225   74  Trachea    NA   NA  VirginFemale Spermatheca    5.5   NA  InseminatedFemale Spermatheca    3   NA  Ovary    438.3   NA  Testis    31.4   NA  Male Accessory Gland    1.6   31.55  Carcass    5.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female  (88) ovary, mated 4-day female  (99) testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female  (88) ovary, mated 4-day female  (99) testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    19 central nervous system, pupae P8    0 head, virgin 1-day female    1 head, virgin 4-day female    0 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    0 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    88 ovary, mated 4-day female    99 testis, mated 4-day male    3 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr  (92) embryo 04-06hr  (90) embryo 06-08hr  (59) embryo 08-10hr  (56) embryo 10-12hr  (31) embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day  (38) adult female 30day  (43) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr  (92) embryo 04-06hr  (90) embryo 06-08hr  (59) embryo 08-10hr  (56) embryo 10-12hr  31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day  (38) adult female 30day  (43) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    92 embryo 04-06hr    90 embryo 06-08hr    59 embryo 08-10hr    56 embryo 10-12hr    31 embryo 12-14hr    21 embryo 14-16hr    15 embryo 16-18hr    14 embryo 18-20hr    7 embryo 20-22hr    12 embryo 22-24hr    14 larva L1    2 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    4 white prepupae 12hr    9 white prepupae 24hr    5 pupae 2d postWPP    2 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    15 adult female 05day    38 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167    175 embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2    199 wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    139 CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS    203 ovary OSC    104 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (89) Schneider line 2 Sg4  (79) embryonic 1182-4H  (81) embryonic GM2  (81) embryonic Kc167  (175) embryonic S1  (42) embryonic S3  (97) leg disc CME L1  (67) wing disc CME-W2  (199) wing disc ML-DmD8  (51) wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (31) wing disc ML-DmD21  (76) wing disc ML-DmD32  (67) haltere disc ML-DmD17-c3  (31) eye-antennal disc ML-DmD11  (54) antennal disc ML-DmD20-c5  (69) mixed discs ML-DmD4-c1  (39) CNS ML-DmBG1-c1  (139) CNS ML-DmBG2-c2  (80) tumorous blood cells mbn2  (74) ovary fGS/OSS  (203) ovary OSC  (104) ovary OSS  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167  (175) embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2  (199) wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1  (139) CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS  (203) ovary OSC  104 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167    175 embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2    199 wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    139 CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS    203 ovary OSC    104 ovary OSS    41 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167    175 embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2    199 wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    139 CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS    203 ovary OSC    104 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (89) Schneider line 2 Sg4  (79) embryonic 1182-4H  (81) embryonic GM2  (81) embryonic Kc167  (175) embryonic S1  (42) embryonic S3  (97) leg disc CME L1  (67) wing disc CME-W2  (199) wing disc ML-DmD8  (51) wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  31 wing disc ML-DmD21  (76) wing disc ML-DmD32  (67) haltere disc ML-DmD17-c3  31 eye-antennal disc ML-DmD11  (54) antennal disc ML-DmD20-c5  (69) mixed discs ML-DmD4-c1  (39) CNS ML-DmBG1-c1  (139) CNS ML-DmBG2-c2  (80) tumorous blood cells mbn2  (74) ovary fGS/OSS  (203) ovary OSC  (104) ovary OSS  (41) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167  (175) embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2  (199) wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1  139 CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS  (203) ovary OSC  104 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    89 Schneider line 2 Sg4    79 embryonic 1182-4H    81 embryonic GM2    81 embryonic Kc167    175 embryonic S1    42 embryonic S3    97 leg disc CME L1    67 wing disc CME-W2    199 wing disc ML-DmD8    51 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    76 wing disc ML-DmD32    67 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    54 antennal disc ML-DmD20-c5    69 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    139 CNS ML-DmBG2-c2    80 tumorous blood cells mbn2    74 ovary fGS/OSS    203 ovary OSC    104 ovary OSS    41 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (65) Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult  (76) Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  (37) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (62) Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (65) Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult  (76) Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  (37) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (62) Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    65 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    76 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    37 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    62 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030501 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11177-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage BthDdsRNA.cMa lethal | P-stage, with Scer\GAL4da.G32 BthDdsRNA.Scer\UAS lethal | P-stage, with Scer\GAL4fkh.PH BthDdsRNA.Scer\UAS partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 BthDGD6665 Other Phenotypes Allele decreased cell size | third instar larval stage, with Scer\GAL4da.G32 BthDdsRNA.Scer\UAS decreased cell size | third instar larval stage, with Scer\GAL4fkh.PH BthDdsRNA.Scer\UAS visible, with Scer\GAL4pnr-MD237 BthDGD6665 Phenotype manifest in Allele embryonic/larval salivary gland, with Scer\GAL4da.G32 BthDdsRNA.Scer\UAS embryonic/larval salivary gland, with Scer\GAL4fkh.PH BthDdsRNA.Scer\UAS mesothoracic tergum, with Scer\GAL4pnr-MD237 BthDGD6665
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of BthD Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of BthD Class Mutagen Stocks Known lesion BthDGD6665 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BthDGLC01456 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BthDcMa in vitro construct 0 Yes BthDdsRNA.cMa in vitro construct - RNAi 0 Yes BthDdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6246 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6665} NA P{TRiP.GLC01456} NA P{UAS-BthD.RNAi} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
larval salivary gland morphogenesis inferred from mutant phenotype (Kwon et al., 2003)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Kwon et al., 2003) Golgi apparatus inferred from direct assay (Kwon et al., 2003)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
selenium binding inferred from electronic annotation with InterPro:IPR011893 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell redox homeostasis inferred from electronic annotation with InterPro:IPR011893 (FlyBase Curators et al., 2004-) response to redox state non-traceable author statement (assigned by UniProtKB) (Martin-Romero et al., 2001)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_redefined_as_selenocysteine (Martin-Romero et al., 2001) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
BthD - Ag5r2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - Arc1 experimental knowledge based (Guruharsha et al., 2011) BthD - Arr1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - CG1129 experimental knowledge based (Guruharsha et al., 2011) BthD - CG1218 experimental knowledge based (Guruharsha et al., 2011) BthD - CG13631 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - CG1532 experimental knowledge based (Guruharsha et al., 2011) BthD - CG18787 experimental knowledge based (Guruharsha et al., 2011) BthD - CG18789 experimental knowledge based (Guruharsha et al., 2011) BthD - CG2540 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) BthD - CG31548 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - CG3706 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - CG4707 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) BthD - CG5746 experimental knowledge based (Guruharsha et al., 2011) BthD - CG7049 experimental knowledge based (Guruharsha et al., 2011) BthD - CTPsyn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - Ccs experimental knowledge based (Guruharsha et al., 2011) BthD - Cpr11B anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) BthD - FK506-bp2 experimental knowledge based (Guruharsha et al., 2011) BthD - Got1 experimental knowledge based (Guruharsha et al., 2011) BthD - RpS21 experimental knowledge based (Guruharsha et al., 2011) BthD - Seipin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - Tsp42Ed anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) BthD - Wdr82 experimental knowledge based (Guruharsha et al., 2011) BthD - knrl anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions BthD allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58694 DroID - A comprehensive database of gene and protein interactions. • FBgn0030501 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32317
Orthologs
OrthoDB Orthologs (9) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61JZZZ Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG6QVR7N
Orthologs in Drosophila Species (EOG61JZZZ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\BthD     Drosophila simulans   Dsim\GD24812 Y   Drosophila erecta   Dere\GG17796 Y   Drosophila yakuba   Dyak\GE17092 Y   Drosophila ananassae   Dana\GF21759 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6QVR7N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Danio rerio Zebrafish  Drer\ENSDARG00000093022   Mus musculus House mouse  Mmus\Selh   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000037534   Homo sapiens Human  Hsap\SELH
Human Orthologs (1)
Gene OMIM HGNC Hsap\SELH
AAA Orthologs (8) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24812 Drosophila erecta Dere\GG17796 Drosophila yakuba Dyak\GE17092 Drosophila ananassae Dana\GF21759 Drosophila willistoni Dwil\GK25075 Drosophila virilis Dvir\GJ19168 Drosophila mojavensis Dmoj\GI15453 Drosophila grimshawi Dgri\GH12971
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	43267 y1 sc* v1; P{TRiP.GLC01456}attP2
VDRC	v45975 w1118; P{GD6665}v45975/TM3
Genomic Clones ( 1 )
	BACR30C16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 29 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD01939 RE28634 SD09114
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM08342 GM15369 GM20987 GM25657 GM25718 LD01631 LD01632 LD29058 RE06729 RE22163 RE46186 RE46219 RE49247 RE51147 RE67713 SD02826 SD17456
Other clones	522529 523672 Dmel_dig_000809_0719_0498 Dmel_dig_002981_0586_2837 EK202809 EN02317 EN04057 EP11048 FGM320H11
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0030501 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32317
Antibody Information
	polyclonal (Kwon et al., 2003)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: BthD CG11177 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of BthD CG11177 was sequence comparison (date:010728). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132675 RefSeq (Proteins) • NP_572903 DDBJ / EMBL / GenBank • AAF48293 AAK72982 AAM51045 AF396455 AI542675 AY119185 Entrez Gene - A searchable database of RefSeq genes. • 32317 UniProtKB/Swiss-Prot • Q9VYB0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Selenoprotein, Rdx type (IPR011893)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58694 DroID - A comprehensive database of gene and protein interactions. • FBgn0030501 DRSC - Results from RNAi screens. • FBgn0030501 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030501 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11177-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030501 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32317 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32317 modMine - Data generated by the modENCODE project. • FBgn0030501
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	BthD (Kwon et al., 2003, Shchedrina et al., 2011, Buffin and Gho, 2010) CG11177 (Dimova et al., 2003, Castellano et al., 2001, Haussmann et al., 2008, Ni et al., 2010.12.1) dselH (Morozova et al., 2003) dSelH (Shchedrina et al., 2011) dselM (Castellano et al., 2001) SelH (Chapple and Guigó, 2008, Takeuchi et al., 2009)
Name Synonym	BthD selenoprotein (Martin-Romero et al., 2001, Buffin and Gho, 2010)
Secondary FlyBase IDs
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 1 )
	Shchedrina et al., 2011, J. Biol. Chem. 286(34): 29449--29461 Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase b1, reveal a role of selenoproteins in stress resistance. [FBrf0214696] Show all research papers ...