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Gene Dmel\hiw

General Information
Symbol	Dmel\hiw	Species	D. melanogaster
Name	highwire	Annotation symbol	CG32592
Feature type	protein_coding_gene	FlyBase ID	FBgn0030600
Gene Model Status	Current	Stock availability	27 publicly available
Genomic Location
Chromosome (arm)	X	Recombination map	1--49.8
Cytogenetic map	13A5-13A8	Sequence location	X:14,904,727..14,957,440 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene highwire is referred to in FlyBase by the symbol Dmel\hiw (CG32592, FBgn0030600). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: negative regulation of synaptic growth at neuromuscular junction; negative regulation of BMP signaling pathway; BMP signaling pathway; autophagy; regulation of synaptic growth at neuromuscular junction; synapse organization. 71 alleles are reported. The phenotypes of these alleles are annotated with: eye; NMJ bouton; bouton; terminal bouton; neuromuscular junction; motor neuron; embryonic/larval neuromuscular junction; indirect flight muscle motor neuron MN4; synapse; synaptic vesicle. It has one annotated transcript and one annotated polypeptide. Protein features are: Anaphase-promoting complex, subunit 10/DOC domain; E3 ubiquitin-protein ligase Highwire/Pam/Rpm-1; Filamin/ABP280 repeat-like; Galactose-binding domain-like; Immunoglobulin E-set; Immunoglobulin-like fold; PHR; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; Regulator of chromosome condensation, RCC1; Zinc finger, RING-type; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-24 hour embryonic stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult midgut. Comments on Affy2 ProbeSet: ProbeSet 1632637_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of hiw. Gene sequence location is X:14904727..14957440.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13A5-13A8   Limits computationally determined from genome sequence between P{EP}EP334&P{EP}EP977 and P{EP}hiwEP1305
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13A5-13A8     (Wan, 2000.5.2) 13A5-13A6   (determined by in situ hybridisation)   (Wan et al., 2000)
Experimentally Determined Recombination Data
Location	1--49.8 (Wan et al., 2000)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\hiw for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) hiw-RA FBtr0073970  NM_078604   16155   5233
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein hiw-PA   FBpp0073787   565.6   5233   6.87     NP_511159   AAF48411
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Regulator of chromosome condensation, RCC1 (IPR000408) Zinc finger, RING-type (IPR001841) PHR (IPR012983) Galactose-binding domain-like (IPR008979) Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II (IPR009091) Immunoglobulin E-set (IPR014756) Anaphase-promoting complex, subunit 10/DOC domain (IPR004939) Filamin/ABP280 repeat-like (IPR017868) E3 ubiquitin-protein ligase Highwire/Pam/Rpm-1 (IPR027047) Zinc finger, RING/FYVE/PHD-type (IPR013083) Immunoglobulin-like fold (IPR013783)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF48411   AF262977 AAF76150   AI296727   AI517666   AQ073177   AQ073179   AY113376 AAM29381   AZ933413   BI582482   BI604568   CO177871   CO264476   CO277273   CO279793   CO321216   CO328584   CO328897   CZ472082   CZ473164   CZ473382   CZ473383   CZ474696   CZ474697   CZ475488   CZ475901   CZ476270   CZ484877   EC051466   EC052415   EC056271   EC072401   EC072523   EC205093   EC216443   EC232793   EY198679   EY198813   GH765665   GH842304   GO271034   GO271129   GO271207
UniProtKB/Swiss-Prot	Q9NB71
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasmic membrane-bounded vesicle inferred from direct assay (Wan et al., 2000) plasma membrane inferred from direct assay (Wan et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult midgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    142.425 Larval Midgut    87.6 Larval Hindgut    28.6 Larval Malpighian Tubules    30.3 Larval Fat Body    16.1 Larval Salivary Gland    13 Larval Trachea    5.525 Larval Carcass    9.7 Adult Head    67.1 Adult Eye    70.85 Adult Brain    322.2 Adult Thoracic-Abdominal Ganglion    301.9 Adult Crop    10.3 Adult Midgut    127.5 Adult Hindgut    33.5 Adult Malpighian Tubules    42 Adult Fat Body    9.7 Adult Salivary Gland    13.3 Adult Heart    5.575 Adult VirginFemale Spermatheca    10.3 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    7.2 Adult Testis    50.8 Adult Male Accessory Gland    9.4 Adult Carcass    13.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    67.1   NA  Eye    70.85   NA  Brain    322.2   142.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    301.9   NA  Crop    no informative data   87.6  Midgut    127.5   28.6  Hindgut    33.5   no informative data  Malpighian Tubules    42   16.1  Fat Body    9.7   no informative data  Salivary Gland    no informative data   NA  Heart    5.575   5.525  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    7.4   NA  Ovary    7.2   NA  Testis    50.8   NA  Male Accessory Gland    9.4   9.7  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    11 central nervous system, pupae P8    8 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    4 head, mated 1-day male    8 head, mated 4-day male    6 head, mated 20-day male    7 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    3 carcass, larvae L3 wandering    2 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-24 hour embryonic stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    2 embryo 06-08hr    5 embryo 08-10hr    8 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    11 embryo 16-18hr    8 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    7 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    11 adult male 30day    11 adult female 01day    4 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    1 embryonic S1    2 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    1 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    0 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_29 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030600 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32592-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable hiwND8 viable, with Scer\GAL4pnr-MD237 hiwGD14101 hiwGD14104 Other Phenotypes Allele decreased cell growth, with Scer\GAL4elav-C155 hiwScer\UAS.fl locomotor behavior defective hiwEMS neuroanatomy defective hiwdsRNA.8667-9272 hiwdsRNA.9806-11038 hiwEMS hiwND8 hiwunspecified hiwΔCterm hiwΔN hiwΔNterm neuroanatomy defective, with Scer\GAL4elav.Switch.PO hiwScer\UAS.T:Zzzz\TAP hiwΔRING.Scer\UAS.T:Zzzz\TAP neuroanatomy defective, with Scer\GAL4elav-C155 hiwScer\UAS.fl hiwScer\UAS.T:Avic\GFP-EGFP neuroanatomy defective (with Df(1)RK2) hiwND9 hiwND16 hiwND17 hiwND36 hiwND42 hiwND45 hiwND48 hiwND59 hiwND69 neuroanatomy defective (with Df(1)RK4) hiwND9 hiwND16 hiwND17 hiwND36 hiwND42 hiwND45 hiwND48 hiwND59 hiwND69 neuroanatomy defective | conditional hiwΔN neuroanatomy defective | larval stage | recessive hiwEMS neuroanatomy defective | somatic clone hiwEMS neuroanatomy defective | third instar larval stage hiwND9 hiwND51 neurophysiology defective hiwEMS hiwND8 hiwΔN Phenotype manifest in Allele bouton hiwND8 hiwΔN bouton | supernumerary hiwEMS embryonic/larval neuromuscular junction hiwND51 hiwND9 hiwΔN embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO, hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwND8 embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO, hiwND8 hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO, hiwΔN.Scer\UAS.T:Zzzz\TAP hiwND8 embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO, hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwND8 embryonic/larval neuromuscular junction, with Scer\GAL4elav.Switch.PO, hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwND8 embryonic/larval neuromuscular junction (with hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC), with Scer\GAL4elav.Switch.PO hiwND8 embryonic/larval neuromuscular junction (with hiwND8), with Scer\GAL4elav.Switch.PO hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP embryonic/larval neuromuscular junction (with hiwΔN.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 embryonic/larval neuromuscular junction (with hiwΔPHR.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 embryonic/larval neuromuscular junction (with hiwΔRCC1.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 eye, with Scer\GAL4GMR.PF hiwGD14101 indirect flight muscle motor neuron MN4 hiwΔNterm hiwΔCterm hiwEMS hiwND8 indirect flight muscle motor neuron MN4 & bouton | ectopic hiwEMS motor neuron (with Df(1)RK4) hiwND8 neuroblast | somatic clone hiwEMS neuromuscular junction hiwΔNterm hiwΔCterm hiwEMS hiwND8 neuromuscular junction, with Scer\GAL4elav.Switch.PO hiwScer\UAS.T:Zzzz\TAP hiwΔRING.Scer\UAS.T:Zzzz\TAP neuromuscular junction, with Scer\GAL4elav-C155 hiwScer\UAS.fl NMJ bouton hiwEMS hiwND51 hiwΔN NMJ bouton, with Scer\GAL4elav.Switch.PO hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP NMJ bouton, with Scer\GAL4elav.Switch.PO, hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwND8 NMJ bouton, with Scer\GAL4elav.Switch.PO, hiwND8 hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP NMJ bouton, with Scer\GAL4elav.Switch.PO, hiwΔN.Scer\UAS.T:Zzzz\TAP hiwND8 NMJ bouton, with Scer\GAL4elav.Switch.PO, hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwND8 NMJ bouton, with Scer\GAL4elav.Switch.PO, hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwND8 NMJ bouton (with hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC), with Scer\GAL4elav.Switch.PO hiwND8 NMJ bouton (with hiwND8), with Scer\GAL4elav.Switch.PO hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP NMJ bouton (with hiwΔN.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 NMJ bouton (with hiwΔPHR.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 NMJ bouton (with hiwΔRCC1.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 NMJ bouton | supernumerary hiwND51 hiwND9 segmental nerve | conditional hiwΔN synapse hiwΔNterm hiwEMS hiwND16 hiwND17 hiwND36 hiwND42 hiwND45 hiwND48 hiwND51 hiwND59 hiwND69 hiwND8 hiwND9 hiwdsRNA.8667-9272 hiwdsRNA.9806-11038 hiwunspecified hiwΔN synapse, with Scer\GAL4elav.Switch.PO hiwScer\UAS.T:Zzzz\TAP hiwΔRING.Scer\UAS.T:Zzzz\TAP hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP synapse, with Scer\GAL4elav.Switch.PO, hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwND8 synapse, with Scer\GAL4elav.Switch.PO, hiwND8 hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP synapse, with Scer\GAL4elav.Switch.PO, hiwΔN.Scer\UAS.T:Zzzz\TAP hiwND8 synapse, with Scer\GAL4elav.Switch.PO, hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwND8 synapse, with Scer\GAL4elav.Switch.PO, hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwND8 synapse, with Scer\GAL4elav-C155 hiwScer\UAS.T:Avic\GFP-EGFP synapse (with hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC), with Scer\GAL4elav.Switch.PO hiwND8 synapse (with hiwND8), with Scer\GAL4elav.Switch.PO hiwΔRCC1.Scer\UAS.T:Zzzz\TAP hiwΔPHR.Scer\UAS.T:Zzzz\TAP hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC hiwΔN.Scer\UAS.T:Zzzz\TAP synapse (with hiwΔN.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 synapse (with hiwΔPHR.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 synapse (with hiwΔRCC1.Scer\UAS.T:Zzzz\TAP), with Scer\GAL4elav.Switch.PO hiwND8 synaptic vesicle hiwunspecified terminal bouton hiwΔNterm hiwΔCterm hiwND8 terminal bouton, with Scer\GAL4elav.Switch.PO hiwScer\UAS.T:Zzzz\TAP hiwΔRING.Scer\UAS.T:Zzzz\TAP
Classical Alleles ( 54 )
For All Classical Alleles Show
Allele of hiw Class Mutagen Stocks Known lesion hiw5-HA-1032 1 -- hiw5-HA-2274 1 -- hiwBG01772 P-element activity 1 -- hiwBG02015 P-element activity 1 Yes hiwc00410 1 -- hiwCB-0441-3 1 -- hiwd01049 1 -- hiwd03079 P-element activity 1 -- hiwd07018 P-element activity 1 -- hiwd08208 1 -- hiwd08512 P-element activity 1 -- hiwEP1305 P-element activity 1 Yes hiwEP1308 P-element activity 1 Yes hiwEY03402 1 -- hiwf02371 piggyBac transposase 1 -- hiwKG01524 P-element activity 1 -- hiwMB02880 1 -- hiwMI02835 1 -- hiwNP2284 1 -- hiwNP4132 1 -- hiwNP7187 1 -- hiwNP7198 1 -- hiw5-HA-1807 0 -- hiwd01196 P-element activity 0 -- hiwd03464 P-element activity 0 -- hiwd05740 P-element activity 0 -- hiwd07738 P-element activity 0 -- hiwd10362 0 -- hiwEMS ethyl methanesulfonate 0 -- hiwEY08069 P-element activity 0 -- hiwGG01154 0 -- hiwGG01225 0 -- hiwGG01292 0 -- hiwM100 spontaneous 0 Yes hiwND16 loss of function allele 0 Yes hiwND17 loss of function allele 0 Yes hiwND36 loss of function allele 0 Yes hiwND42 loss of function allele 0 Yes hiwND45 loss of function allele 0 -- hiwND48 loss of function allele 0 -- hiwND51 hypomorphic allele - genetic evidence 0 Yes hiwND55 hypomorphic allele - genetic evidence 0 -- hiwND59 loss of function allele 0 -- hiwND69 loss of function allele 0 Yes hiwND8 loss of function allele 0 Yes hiwND9 loss of function allele 0 Yes hiwNP4013 0 -- hiwNP4091 0 -- hiwPF253 P-element activity 0 Yes hiwunspecified 0 -- hiwΔC P-element activity 0 Yes hiwΔCterm P-element activity 0 Yes hiwΔN P-element activity 0 Yes hiwΔNterm P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of hiw Class Mutagen Stocks Known lesion hiwGD14101 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes hiwGD14104 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hiwJF02866 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes hiwCT1000.Scer\UAS.T:Zzzz\TAP in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwdsRNA.8667-9272 in vitro construct - RNAi 0 Yes hiwdsRNA.9806-11038 in vitro construct - RNAi 0 Yes hiwHindIII.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwScer\UAS.fl in vitro construct - regulatory fusion 0 Yes hiwScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion 0 Yes hiwScer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes hiwScer\UAS.W.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes hiwΔC.Scer\UAS.T:Zzzz\TAP in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwΔN.Scer\UAS.T:Zzzz\TAP in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwΔPHR.Scer\UAS.T:Zzzz\TAP in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwΔRCC1.Scer\UAS.T:Zzzz\TAP in vitro construct - deletion in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes hiwΔRING.Scer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes hiwΔRING.Scer\UAS in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)RK2 (Wan et al., 2000) Df(1)RK4 (Wan et al., 2000)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14101} NA P{GD14104} NA P{TRiP.JF02866} NA P{UAS-GFP-hiw} NA P{UAS-hiw.fl} NA P{UAS-hiw.W} NA P{UAS-hiw.ΔRING} NA P{UAS-HM-Hiw-HindIII} NA P{UAS-NTAP-hiw.CT1000} NA P{UAS-NTAP-hiw.ΔC} NA P{UAS-NTAP-hiw.ΔN} NA P{UAS-NTAP-hiw.ΔPHR} NA P{UAS-NTAP-hiw.ΔRCC1} NA P{UAS-TAP-hiw} NA P{UAS-TAP-hiwΔRING} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}hiwEP1305 NA P{EP}hiwEP1308 NA P{EPgy2}beEY08069 NA P{EPgy2}hiwEY03402 NA P{GSV1}hiwPF253 NA P{Mae-UAS.6.11}B0578B0578 NA P{Mae-UAS.6.11}B2019B2019 NA P{Mae-UAS.6.11}hiwGG01154 NA P{Mae-UAS.6.11}hiwGG01225 NA P{Mae-UAS.6.11}hiwGG01292 NA P{XP}hiwd01049 NA P{XP}hiwd01196 NA P{XP}hiwd01497 NA P{XP}hiwd03079 NA P{XP}hiwd03464 NA P{XP}hiwd05740 NA P{XP}hiwd07018 NA P{XP}hiwd07738 NA P{XP}hiwd08208 NA P{XP}hiwd08512 NA P{XP}hiwd10362 NA miscellaneous insertions Mi{MIC}beMI02835 NA P{GawB}beNP4013 NA P{GT1}hiwBG01772 NA P{GT1}hiwBG02015 NA P{RS3}hiwCB-0441-3 NA P{RS5}hiw5-HA-1032 NA P{RS5}hiw5-HA-1807 NA P{RS5}hiw5-HA-2274 NA P{SUPor-P}hiwKG01524 NA PBac{PB}hiwc00410 NA PBac{WH}hiwf02371 NA insertion of enhancer trap binary system Mi{ET1}hiwMB02880 No P{GawB}hiwNP2284 No P{GawB}hiwNP4091 No P{GawB}hiwNP4132 No P{GawB}hiwNP7187 No P{GawB}hiwNP7198 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Fsn (Tian et al., 2011) inferred from physical interaction with Med (assigned by UniProtKB) (McCabe et al., 2004) inferred from physical interaction with Rae1 (Tian et al., 2011)
Biological Process
CV term Evidence References
autophagy inferred from direct assay (Shen and Ganetzky, 2009) BMP signaling pathway inferred from physical interaction with Med (assigned by UniProtKB) (McCabe et al., 2004) negative regulation of BMP signaling pathway inferred from genetic interaction with Med AND inferred from genetic interaction with wit (assigned by UniProtKB) (McCabe et al., 2004) negative regulation of synaptic growth at neuromuscular junction inferred from mutant phenotype (assigned by UniProtKB) (McCabe et al., 2004) regulation of synaptic growth at neuromuscular junction inferred from mutant phenotype (Wan et al., 2000, Wu et al., 2005) synapse organization inferred from direct assay (Shen and Ganetzky, 2009)
Cellular Component
CV term Evidence References
cytoplasmic membrane-bounded vesicle inferred from direct assay (Wan et al., 2000) plasma membrane inferred from direct assay (Wan et al., 2000)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ubiquitin-protein ligase activity inferred from sequence or structural similarity with UniProtKB:Q8WY64 (assigned by UniProtKB) (UniProtKB Project Members, 2005.6.21) zinc ion binding inferred from electronic annotation with InterPro:IPR001841 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
adult walking behavior non-traceable author statement (Strauss, 2002) locomotion non-traceable author statement (Strauss, 2002) protein ubiquitination inferred from sequence or structural similarity with UniProtKB:Q8WY64 (assigned by UniProtKB) (UniProtKB Project Members, 2005.6.21) regulation of synaptic growth at neuromuscular junction traceable author statement (Strauss, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
hiw - CG11455 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions hiw allele Gene References hiwEMS gig (Murthy et al., 2004) Med (McCabe et al., 2004) wit (McCabe et al., 2004) hiwEP1305 gig (Murthy et al., 2004) hiwND8 gig (Murthy et al., 2004) hiwND42 gig (Murthy et al., 2004) hiwND69 gig (Murthy et al., 2004) hiwunspecified faf (DiAntonio et al., 2001) unspecified Tsc1 (Murthy et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58787 DroID - A comprehensive database of gene and protein interactions. • FBgn0030600 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32429
Orthologs
OrthoDB Orthologs (51) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BP1X1 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6PZH2F Arthropod inclusive ortholog search EOG6WDBSN Metazoa inclusive ortholog search EOG6N93XB
Orthologs in Drosophila Species (EOG6BP1X1)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\hiw     Drosophila simulans   Dsim\GD15284     Drosophila simulans   Dsim\GD15825 Y   Drosophila sechellia   Dsec\GM25893     Drosophila sechellia   Dsec\GM24877     Drosophila sechellia   Dsec\GM17172     Drosophila sechellia   Dsec\GM12011     Drosophila sechellia   Dsec\GM15269     Drosophila sechellia   Dsec\GM19036     Drosophila erecta   Dere\GG14254     Drosophila erecta   Dere\GG19425 Y   Drosophila erecta   Dere\GG24258     Drosophila yakuba   Dyak\GE16076 Y   Drosophila ananassae   Dana\GF22072 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA28844     Drosophila pseudoobscura pseudoobscura   Dpse\GA17013 Y   Drosophila persimilis   Dper\GL26848     Drosophila persimilis   Dper\GL12869     Drosophila willistoni   Dwil\GK19733 Y   Drosophila virilis   Dvir\GJ16794 Y   Drosophila mojavensis   Dmoj\GI15369     Drosophila mojavensis   Dmoj\GI15368     Drosophila grimshawi   Dgri\GH11951 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6PZH2F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB42147   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007148   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17251   Atta cephalotes Leafcutter ant  Acep\ACEP27835   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14432   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13135   Linepithema humile Argentine ant  Lhum\LH21412   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21217   Solenopsis invicta Red fire ant  Sinv\SINV25096   Acyrthosiphon pisum Pea aphid  Apim\ACYPI40372   Acyrthosiphon pisum Pea aphid  Apim\ACYPI088596   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002603   Bombyx mori Silkmoth  Bmor\BGIBMGA012339   Pediculus humanus Human body louse  Phum\PHUM483780   Tribolium castaneum Red flour beetle  Tcas\TC003207
Orthologs in non-Insect Arthropods (EOG6WDBSN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\nad5   Daphnia pulex Water flea  Dpux\Sam-S   Ixodes scapularis Deer tick  Isca\ISCW011859
Orthologs in non-Arthropod Metazoa (EOG6N93XB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rpm-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008030gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004269gn   Danio rerio Zebrafish  Drer\mycbp2   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000015459   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031605   Gallus gallus Domestic chicken  Ggal\MYCBP2   Mus musculus House mouse  Mmus\Mycbp2   Rattus norvegicus Norway rat  Rnor\Mycbp2   Homo sapiens Human  Hsap\ENSG00000005810
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000005810
AAA Orthologs (8) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15825 Drosophila erecta Dere\GG19425 Drosophila yakuba Dyak\GE16076 Drosophila ananassae Dana\GF22072 Drosophila pseudoobscura pseudoobscura Dpse\GA17013 Drosophila willistoni Dwil\GK19733 Drosophila virilis Dvir\GJ16794 Drosophila grimshawi Dgri\GH11951
Stocks & Reagents
Stocks Listed in FlyBase ( 27 )
Bloomington	11420 w1118 P{EP}hiwEP1305
Harvard	- P{XP}hiwd01049 - P{XP}hiwd03079 - P{XP}hiwd07018 - P{XP}hiwd08208 - P{XP}hiwd08512 - PBac{PB}hiwc00410 - PBac{WH}hiwf02371
VDRC	v26998 w1118; P{GD14101}v26998 v28163 w1118; P{GD14101}v28163 v36085 w1118; P{GD14104}v36085 v40630 w1118; P{GD14101}v40630/CyO
	More stocks available...
Genomic Clones ( 1 )
	BACR10J19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 25 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP10764
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH28713 RH20644 RH69982
Other clones	16268 28073 30366 211108 211276.1 DMG2-chrX.15.076.a.1 DMG2-chrX.15.079.a DMG7-chrX.26.004.pred.a EC051466 EC052415 EC056271 EK054545 EK123710 EK136034 EK139346 EK290523 EK290940 EK291622 FB59-CG32592-RA RP001051702 RP003566763
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0030600 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32429
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: hiw CG9041 CG9049 (FlyBase, 1996-)
Source for database merge of
Additional comments	Annotations CG9041, CG9049 merged as CG32592 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for identity of hiw CG9041 CG9049 was sequence comparison (date:000622). (FlyBase, 1996-)
Other Comments
	hiw functions to control wnd primarily in larva. (Collins et al., 2006) hiw regulates synaptic physiology through a distinct molecular pathway that does not require wnd. (Collins et al., 2006) Synaptic overgrowth in hiw mutants requires JNK signalling. (Collins et al., 2006) The RING domain of hiw plays an essential role in regulating synaptic function. (Wu et al., 2005) hiw dependant ubiquitination has a role in controlling the level of activity of critical regulatory molecules at the synapse. (DiAntonio et al., 2001) hiw appears to function as a negative regulator of synaptic growth. (Wan et al., 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078604 RefSeq (Proteins) • NP_511159 DDBJ / EMBL / GenBank • AAF48411 AAF76150 AAM29381 AF262977 AI296727 AQ073177 AQ073179 AY113376 AZ933413 BI604568 CZ472082 CZ473164 CZ473382 CZ473383 CZ474696 CZ474697 CZ475488 CZ475901 CZ476270 CZ484877 Entrez Gene - A searchable database of RefSeq genes. • 32429 UniProtKB/Swiss-Prot • Q9NB71
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Regulator of chromosome condensation, RCC1 (IPR000408) Zinc finger, RING-type (IPR001841) PHR (IPR012983) Galactose-binding domain-like (IPR008979) Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II (IPR009091) Immunoglobulin E-set (IPR014756) Anaphase-promoting complex, subunit 10/DOC domain (IPR004939) Filamin/ABP280 repeat-like (IPR017868) E3 ubiquitin-protein ligase Highwire/Pam/Rpm-1 (IPR027047) Zinc finger, RING/FYVE/PHD-type (IPR013083) Immunoglobulin-like fold (IPR013783)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58787 DroID - A comprehensive database of gene and protein interactions. • FBgn0030600 DRSC - Results from RNAi screens. • FBgn0030600 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030600 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32592-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030600 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32429 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/highwir1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32429 modMine - Data generated by the modENCODE project. • FBgn0030600
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG9041   CG9049   CG32592 (Grieder et al., 2007, Perkins et al., 2009, Awofala et al., 2012) hiw (UniProtKB Project Members, 2005.6.21, Haag-Liautard et al., 2007, Murphey and Godenschwege, 2002, Marques, 2005, Massaro et al., 2009, Milton et al., 2011, Tian et al., 2011, Kuo et al., 2006, Uthaman et al., 2008, Zhao et al., 2009, Mozer and Sandstrom, 2012, Shen and Ganetzky, 2009, Doumanis et al., 2009, Merino et al., 2009, Xiong et al., 2010, Wasbrough et al., 2010, Bao et al., 2008, Miller et al., 2012, Awofala et al., 2012, Brink et al., 2012) Hiw (Bao et al., 2007, Iida et al., 2007, Martin et al., 2007, Haussmann et al., 2008, Doumanis et al., 2009)
Name Synonym	highwire (Wan, 2000.5.2, Collins et al., 2006, Wu et al., 2005, Murphey and Godenschwege, 2002, Marques, 2005, Massaro et al., 2009, Uthaman et al., 2008, Wu et al., 2007, Shen and Ganetzky, 2009, Merino et al., 2009, Harvey et al., 2008, Hong et al., 2012, Miller et al., 2012) Highwire (Bao et al., 2007, Godena et al., 2011, Tian et al., 2011, Haussmann et al., 2008, Soustelle and Giangrande, 2007, Awofala et al., 2012)
Secondary FlyBase IDs
	FBgn0030604 FBgn0052592
References ( 76 )
Generate a list of	All references relating to this gene ( 76 )
List References by type	Research paper  ( 36 ) Supplementary material  ( 5 ) Review  ( 15 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 1 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 9 )
	Awofala et al., 2012, Funct. Integr. Genomics 12(2): 305--315 Functional roles for redox genes in ethanol sensitivity in Drosophila. [FBrf0218408] Brink et al., 2012, PLoS ONE 7(5): e37876 Glial processes at the Drosophila larval neuromuscular junction match synaptic growth. [FBrf0218488] Hong et al., 2012, G3 (Bethesda) 2(8): 873--882 Microarray-Based Capture of Novel Expressed Cell Type-Specific Transfrags (CoNECT) to Annotate Tissue-Specific Transcription in Drosophila melanogaster. [FBrf0219214] Miller et al., 2012, J. Neurosci. 32(40): 13776--13786 Analysis of synaptic growth and function in Drosophila with an extended larval stage. [FBrf0219554] Mozer and Sandstrom, 2012, Mol. Cell. Neurosci. 51(3-4): 89--100 Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase. [FBrf0219839] Godena et al., 2011, PLoS ONE 6(3): e17808 TDP-43 Regulates Drosophila Neuromuscular Junctions Growth by Modulating Futsch/MAP1B Levels and Synaptic Microtubules Organization. [FBrf0213270] Milton et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(42): 17521--17526 Oxidative stress induces overgrowth of the Drosophila neuromuscular junction. [FBrf0216440] Shin and Diantonio, 2011, J. Neurosci. 31(48): 17689--17700 Highwire regulates guidance of sister axons in the Drosophila mushroom body. [FBrf0216839] Tian et al., 2011, Nat. Neurosci. 14(10): 1267--1275 Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons. [FBrf0216253] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...