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Gene Dmel\Graf

General Information
Symbol	Dmel\Graf	Species	D. melanogaster
Name	Graf	Annotation symbol	CG8948
Feature type	protein_coding_gene	FlyBase ID	FBgn0030685
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	13E14-13E14	Sequence location	X:15,645,707..15,654,741 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Graf (CG8948, FBgn0030685). It has the cytological map location 13E14. Its sequence location is X:15645707..15654741. Its molecular function is described as: oxidoreductase activity; Rho GTPase activator activity. It is involved in the biological processes: metabolic process; signal transduction. 10 alleles are reported. No phenotypic data is available. It has 6 annotated transcripts and 6 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13E14-13E14   Limits computationally determined from genome sequence between P{EP}EP1489 and P{EP}BEST:RE35685EP1581&P{EP}sdEP1088
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Graf for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Graf-RA FBtr0074194  NM_132842   4136   1016 Graf-RB FBtr0074195  NM_206722   3962   1016 Graf-RC FBtr0074196  NM_206721   4178   1016 Graf-RD FBtr0074197  NM_206720   4421   1016 Graf-RE FBtr0074198  NM_206719   4261   1016 Graf-RF FBtr0074199  NM_206718   3947   1016
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Graf-PA   FBpp0073979   114.7   1016   8.26     NP_573070   AAF48506 Graf-PB   FBpp0089210   114.7   1016   8.26     NP_996445   AAS65342 Graf-PC   FBpp0089211   114.7   1016   8.26     NP_996444   AAS65343 Graf-PD   FBpp0089212   114.7   1016   8.26     NP_996443   AAS65344 Graf-PE   FBpp0089213   114.7   1016   8.26     NP_996442   AAS65345 Graf-PF   FBpp0089209   114.7   1016   8.26     NP_996441   AAS65346
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0030685
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RhoGAP (IPR000198) Src homology-3 (IPR001452) Pleckstrin-like (IPR001849) Chromogranin/secretogranin (IPR001990) Short-chain dehydrogenase/reductase SDR (IPR002198) Rho GTPase activation protein (IPR008936) Pleckstrin homology-type (IPR011993)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263356   AA390527   AA698084   AA949947   AA949951   AA979654   AE003500 AAF48506   AE003500 AAS65342   AE003500 AAS65343   AE003500 AAS65344   AE003500 AAS65345   AE003500 AAS65346   AI403993   AI405791   AI532880   AI533853   AI533909   AW942893   BF487903   BG637859   BG639691   BI162001   BI162002   BI165162   BI173624   BI373481   BI634036   BP545379   BP545380   BP559136   BP559137   BQ103290   BT003577 AAO39581   CK660352   CL526115   CO155675   CO186295   CO187012   CO190089   CO196419   CO281904   CO292606   CO295297   CO300302   CO305497   CO309085   CO322961   CO331380   CO333283   CO338331   CO340640   CZ470120   CZ478485   CZ479455   CZ479468   CZ481111   CZ485515   EC056791   EC065941   EC067652   EC068995   EC091142   EC091144   EC194931   EC206055   EC215134   EC218249   EC222744   EC228915   EC229540   EC235085   EC240607   EC242159   EC243988   EC247020   EC247566   EC254648   EC261650   EC262044   EC263110   RE01436   SD04454
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q86NY4 Q9VXQ2
Maps to
Does NOT map to
Identified with	LD28528 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2938
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI532828 AA949951 AI532978
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030685 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0030685 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0030685
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable GrafEY09461 Sterility Allele fertile GrafEY09461 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Graf Class Mutagen Stocks Known lesion GrafEY09461 P-element activity 1 -- Grafc05593 piggyBac transposase 0 -- Grafe00621 piggyBac transposase 1 -- Grafe01650 piggyBac transposase 0 -- Grafe01661 piggyBac transposase 0 -- Grafe03337 piggyBac transposase 1 -- Graff02954 piggyBac transposase 1 -- Graf[MB01672] 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Graf Class Mutagen Stocks Known lesion GrafGD14077 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes GrafdsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 2 Yes
Aneuploid Aberrations
Useful deficiency	T(1;3)143-3
Useful duplication	Dp(1;1)M48.6
Disrupted in	Df(1)Exel6250 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14077} NA P{UAS-Graf-dsRNA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}GrafEY09461 NA miscellaneous insertions PBac{PB}Grafc05593 NA PBac{RB}Grafe00621 NA PBac{RB}Grafe01650 NA PBac{RB}Grafe01661 NA PBac{RB}Grafe03337 NA PBac{WH}Graff02954 NA insertion of enhancer trap binary system Mi{ET1}GrafMB01672 No Mi{ET1}GrafMB01672b No
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 5 )
Molecular Function
CV term Evidence References
Rho GTPase activator activity inferred from sequence or structural similarity with UniProt:Q9UNA1 (FlyBase, 1992-)
oxidoreductase activity inferred from electronic annotation with InterPro:IPR002198 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
metabolic process inferred from electronic annotation with InterPro:IPR002198 (FlyBase Curators et al., 2004-)
signal transduction inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Graf allele Gene References unspecified trc (He et al., 2005)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58871 Drosophila PIMRider - The Drosophila Protein Interaction map • 521994
Orthologs
Genome-wide drosophilid orthologs	Dpse\GA24002 Dere\GG19375 Dmoj\GI14824 Dper\GL15356 Dsec\GM22529 Dsim\GD24498 Dvir\GJ19489 Dwil\GK25279 Dyak\GE16023
Curated drosophilid orthologs	Dpse\GA21428
Linkouts	InParanoid orthologs - Eukaryotic orthologs • MGI:1918552 Mus musculus (Informatics) DDB0215295 Dictyostelium discoideum (Dictybase) ZDB-GENE-030131-8077 Danio rerio (Zfin) WBGene00020209 Caenorhabditis elegans (Wormbase) WBGene00030696 Caenorhabditis briggsae (Wormbase) ENSXETP00000040872 Xenopus tropicalis (Ensembl) GSTENP00033768001 Tetraodon nigroviridis (Ensembl) ENSRNOP00000036940 Rattus norvegicus (Ensembl) ENSPTRP00000029677 Pan troglodytes (Ensembl) ENSMODP00000012986 Monodelphis domestica (Ensembl) ENSMMUP00000000564 Macaca mulatta (Ensembl) ENSP00000274498 Homo sapiens (Ensembl) ENSGALP00000011921 Gallus gallus (Ensembl) NEWSINFRUP00000133821 Takifugu rubripes (Ensembl) ENSCINP00000007410 Ciona intestinalis (Ensembl) ENSCAFP00000009426 Canis familiaris (Ensembl) ENSBTAP00000029813 Bos taurus (Ensembl) ENSANGP00000021464 Anopheles gambiae (Ensembl)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Harvard	- PBac{RB}Grafe00621 - PBac{RB}Grafe03337 - PBac{WH}Graff02954
VDRC	v42165 w1118; P{GD14077}v42165 v42166 w1118; P{GD14077}v42166/TM3
Bloomington	23339 w1118 Mi{ET1}GrafMB01672 6425 y1 w*; P{UAS-Graf-dsRNA}4 6426 y1 w*; P{UAS-Graf-dsRNA}5.1 17571 y1 w67c23 P{EPgy2}GrafEY09461
Genomic Clones ( 2 )
	BACR22H11 BACR25C18
cDNA Clones ( 35 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD28528
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD05824 LP18855 RE04985 LD34359 GH25920 LD28522 SD05893 HL03753 LD08744 RE16582 GH23558 RE60777 SD04522 AT23010 SD28269
Other clones	SD04454 EK299438 EK189312 EK248821 EK159048 RE01436 EK177907 UUGC0693 EK018519 EK200556 EK209348 EK046459 EK004228 EN16520 EK306407 EK260236 EP11040 EN06539 EK025312
RNAi & Array Information
Affy Oligo