Gene Dmel\Traf-like

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General Information

Symbol

Dmel\Traf-like

Species

D. melanogaster

Name

TNF-receptor-associated factor-like

Annotation symbol

CG4394

Feature type

protein_coding_gene

FlyBase ID

FBgn0030748

Gene Model Status

Current

Stock availability

3 publicly available

Also Known As

TRAF3, dTRAF3

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

14C4-14C4

Sequence location

X:16,309,079..16,311,618 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene TNF-receptor-associated factor-like is referred to in FlyBase by the symbol Dmel\Traf-like (CG4394, FBgn0030748). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. Based on sequence similarity, it is predicted to be involved in the biological process: signal transduction; defense response. 3 alleles are reported. No phenotypic data is available. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: MATH; TRAF-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart. Comments on Affy2 ProbeSet: ProbeSet 1633359_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Traf-like. Gene sequence location is X:16309079..16311618.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Controlled Vocabulary Terms

molecular_function

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14C4-14C4

Limits computationally determined from genome sequence between P{EP}EP1493^EP1493 and P{EP}EP1547^EP1547

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Traf-like for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Traf-like-RA

FBtr0074275

NM_167517

2285

474

Traf-like-RB

FBtr0074274

NM_167518

1829

395

Traf-like-RC

FBtr0074273

NM_132898

2356

452

Traf-like-RD

FBtr0308606

NM_001258784

1877

395

Traf-like-RE

FBtr0308607

NM_001258785

2275

395

Traf-like-RF

FBtr0334036

NM_001272683

2227

395

Traf-like-RG

FBtr0334037

NM_001272684

1878

395

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Traf-like-PA

FBpp0074051

55.9

474

6.30

NP_727976

AAN09408

Traf-like-PB

FBpp0074050

46.7

395

6.26

NP_727977

AAF48607

Traf-like-PC

FBpp0074049

53.6

452

6.51

NP_573126

AAF48608

Traf-like-PD

FBpp0300830

46.7

395

6.26

NP_001245713

AFH07426

Traf-like-PE

FBpp0300831

46.7

395

6.26

NP_001245714

AFH07427

Traf-like-PF

FBpp0306152

46.7

395

6.26

NP_001259612

AGB95454

Traf-like-PG

FBpp0306153

46.7

395

6.26

NP_001259613

AGB95455

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

MATH (IPR002083)

TRAF-like (IPR008974)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

garland cell primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		(809.2)
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		28.525
Larval Midgut		535.6
Larval Hindgut		176.6
Larval Malpighian Tubules		809.2
Larval Fat Body		43.4
Larval Salivary Gland		58.7
Larval Trachea		15.35
Larval Carcass		36.125
Adult Head		18.4
Adult Eye		9.9
Adult Brain		9.5
Adult Thoracic-Abdominal Ganglion		15.6
Adult Crop		24.8
Adult Midgut		423.3
Adult Hindgut		113.6
Adult Malpighian Tubules		382.6
Adult Fat Body		47.3
Adult Salivary Gland		60.5
Adult Heart		304.1
Adult VirginFemale Spermatheca		18.3
Adult InseminatedFemale Spermatheca		23.7
Adult Ovary		10.7
Adult Testis		3.9
Adult Male Accessory Gland		68.5
Adult Carcass		25.9
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	18.4
NA	Eye	9.9
NA	Brain	9.5
28.525	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	15.6
NA	Crop	24.8
535.6	Midgut	423.3
176.6	Hindgut	113.6
809.2	Malpighian Tubules	382.6
43.4	Fat Body	47.3
58.7	Salivary Gland	60.5
NA	Heart	304.1
15.35	Trachea	NA
NA	VirginFemale Spermatheca	18.3
NA	InseminatedFemale Spermatheca	23.7
NA	Ovary	10.7
NA	Testis	3.9
NA	Male Accessory Gland	68.5
36.125	Carcass	25.9

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(34)
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(34)
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		34
digestive system, 1-day adult		17
digestive system, 4-day adult		15
digestive system, 20-day adult		17
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		(30)
embryo 18-20hr		(52)
embryo 20-22hr		(42)
embryo 22-24hr		(52)
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		(52)
embryo 20-22hr		(42)
embryo 22-24hr		(52)
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		2
embryo 12-14hr		3
embryo 14-16hr		10
embryo 16-18hr		30
embryo 18-20hr		52
embryo 20-22hr		42
embryo 22-24hr		52
larva L1		11
larva L2		9
larva L3 12hr old		8
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		20
larva L3 puffstage 7-9		22
white prepupae new		12
white prepupae 12hr		20
white prepupae 24hr		5
pupae 2d postWPP		3
pupae 3d postWPP		2
pupae 4d postWPP		7
adult male 01day		6
adult male 05day		6
adult male 30day		5
adult female 01day		6
adult female 05day		2
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		(35)
leg disc CME L1		(31)
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		(35)
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		1
Schneider line 2 Sg4		16
embryonic 1182-4H		3
embryonic GM2		26
embryonic Kc167		20
embryonic S1		2
embryonic S3		35
leg disc CME L1		31
wing disc CME-W2		24
wing disc ML-DmD8		1
wing disc ML-DmD9		2
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		2
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		5
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		7
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		5
heat shock, 4-day adult		3
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		11
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		4
Zinc 4.5 mM 48 hrs, 4-day adult		4
Ethanol 2.5% 3 hrs, larvae L3		3
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		6
Caffeine 1.5 mg/ml 4 hrs, larvae L3		12
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		3
Caffeine 25 mg/ml 48 hrs, 4-day adult		4
Paraquat 5 mM 48 hrs, 4-day adult		5
Paraquat 10 mM 48 hrs, 4-day adult		4
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

External Data & Images

Linkouts

BDGP expression data

•

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0030748

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4394-RC

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Traf-like

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Traf-like^GD11301

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Traf-like	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Traf-like	Mutagen	Stocks	Known lesion
Traf-like^GD11301	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Traf-like^HMJ02121	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Traf-like^dsRNA.cSa	in vitro construct - RNAi	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{TRiP.HMJ02121}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

There is no information supporting a molecular function term for this gene.

(Based on a review of available data on: 2013-03-07)

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

defense response

inferred from sequence or structural similarity with EMBL:U33840

(FlyBase, 1992-)

signal transduction

inferred from sequence or structural similarity with EMBL:U33840

(FlyBase, 1992-)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from sequence or structural similarity with Traf4

(Curators, 2008--)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Traf-like allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

58951

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0030748

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32611

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q8IR14

Orthologs

OrthoDB Orthologs (18) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6PZJSG

Dipteran inclusive ortholog search

EOG63216N

Insect inclusive ortholog search

EOG6N5TS8

Arthropod inclusive ortholog search

EOG6SQVC2

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6PZJSG)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18154

Drosophila persimilis

Dper\GL15839

Drosophila willistoni

Dwil\GK25213

Drosophila virilis

Dvir\GJ18797

Drosophila mojavensis

Dmoj\GI14463

Drosophila grimshawi

Dgri\GH12698

Orthologs in non-Drosophila Dipterans (EOG63216N)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011363

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015342

Anopheles gambiae

Malaria mosquito

Agam\TRAF6

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010862

Orthologs in non-Dipteran Insects (EOG6N5TS8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009461

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07710

Orthologs in non-Insect Arthropods (EOG6SQVC2)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW001705

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18154

Drosophila persimilis

Dper\GL15839

Drosophila willistoni

Dwil\GK25213

Drosophila virilis

Dvir\GJ18797

Drosophila mojavensis

Dmoj\GI14463

Drosophila grimshawi

Dgri\GH12698

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

42549

y¹ v¹; P{TRiP.HMJ02121}attP40

VDRC

v34835

w¹¹¹⁸; P{GD11301}v34835

v34836

w¹¹¹⁸; P{GD11301}v34836

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 114 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones