FlyBase Gene Report: Dmel\Frq1

General Information

Symbol

Dmel\Frq1

Species

D. melanogaster

Name

Frequenin 1

Annotation Symbol

CG5744

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030897

Gene Model Status

Current

Stock Availability

45 publicly available

Gene Summary

Ca(2+)-dependent modulation of synaptic efficacy (PubMed:17970740, PubMed:8101711). Also plays a role in axon terminal morphology (PubMed:17970740). (UniProt, P37236)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

Frq, frequenin, HL-II

Key Links

Genomic Location

Cytogenetic map

16F7-17A1

Sequence location

X:18,155,369..18,174,744 [+]

Recombination map

1-59

RefSeq locus

NC_004354 REGION:18155369..18174744

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from direct assay

(Pongs et al., 1993)

enables calcium sensitive guanylate cyclase activator activity

inferred from direct assay

(Pongs et al., 1993)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from biological aspect of ancestor with PANTHER:PTN000550679

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR028846

(InterPro Project Members, 2004-)

enables calcium sensitive guanylate cyclase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN001128302

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P35243

(Pongs et al., 1993)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in chemical synaptic transmission

inferred from mutant phenotype

(Romero-Pozuelo et al., 2007)

involved_in neuromuscular junction development

inferred from mutant phenotype

(Romero-Pozuelo et al., 2007)

involved_in neurotransmitter secretion

inferred from mutant phenotype

(Pongs et al., 1993)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of neurotransmitter secretion

inferred from sequence or structural similarity with FLYBASE:Frq2; FB:FBgn0083228

(Dason et al., 2009)

involved_in regulation of signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008588905

(GO Reference Genome Project, 2011-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the recoverin family. (P37236)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Ca(2+)-dependent modulation of synaptic efficacy (PubMed:17970740, PubMed:8101711). Also plays a role in axon terminal morphology (PubMed:17970740).

(UniProt, P37236)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Frq1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P37236)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Frq1-RB

FBtr0074545

NM_133043

5686

187

Frq1-RC

FBtr0100239

NM_001038764

5732

187

Frq1-RD

FBtr0335119

NM_001272744

5594

187

Frq1-RE

FBtr0335120

NM_001272745

5590

187

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Frq1-PB

FBpp0074319

21.7

187

4.75

FBpp0099625

21.7

187

4.75

FBpp0307120

21.7

187

4.75

FBpp0307121

21.7

187

4.75

P37236

NP_001259674

AGB95516

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

187 aa isoforms: Frq1-PB, Frq1-PC, Frq1-PD, Frq1-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

In contrast to Frq2, does not interact with ric8a.

(UniProt, P37236)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Frq1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Frq1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.70

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Pongs et al., 1993)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

embryonic stage 17

larval ventral nerve cord

(Romero-Pozuelo et al., 2007)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Romero-Pozuelo et al., 2007)

first instar larval stage

organism

(Romero-Pozuelo et al., 2007)

adult stage

organism

(Romero-Pozuelo et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

(Pongs et al., 1993)

central nervous system

(Pongs et al., 1993)

larval stage

larval brain

(Pongs et al., 1993)

neuromuscular junction

(Pongs et al., 1993)

adult stage

lamina

(Pongs et al., 1993)

adult brain

(Pongs et al., 1993)

synapse

(Pongs et al., 1993)

neuromuscular junction

(Pongs et al., 1993)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Frq protein is detected at low levels in embryos, third instar larvae, and pupae, and at high levels in adults on Western blots. Immunolocalization shows that Frq protein is localized mainly to the nervous system. In late embryos, it is expressed in distinct groups of cells in the CNS and in some neurons of the PNS. In larvae and adults, it is detected mainly in synaptic regions, especially in adult brain and in larval and adult neuromuscular junctions. Staining is particularly intense in the synapses of motor nerves innervating larval or adult muscle. At higher magnification, staining is also seen in the adult brain in neuropil areas and in some cells (probably glia) in the lamina.

(Pongs et al., 1993)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Frq1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Frq1

View exonic expression by tissue for Dmel\Frq1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030897

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32797/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030897

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030897

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030897

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5744

Flygut - An atlas of the Drosophila adult midgut

FBgn0030897

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Frq1

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Frq1

Transgenic constructs containing regulatory region of Frq1

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(1)frq^del1

(Dason et al., 2009)

Df(1)frq^del3

(Dason et al., 2009)

T(1;Y)V7

(Pongs et al., 1993)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Frq1^GD13502

viable, with Scer\GAL4^Mef2.PR

Frq1^GD13502

viable, with Scer\GAL4^pnr-MD237

Frq1^GD13502

viable, with Scer\GAL4^tin.CΔ4

Frq1^GD13502

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

Frq1^DN.UAS

abnormal neuroanatomy, with Scer\GAL4^elav-C155

Frq1^UAS.cRPa

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

Frq1^UAS.cRPa

abnormal neuroanatomy | larval stage, with Scer\GAL4^Toll-6-D42

abnormal neuroanatomy | larval stage | heat sensitive

Frq1^hs.PP

abnormal neurophysiology, with Scer\GAL4^elav-C155

Frq1^UAS.cRPa

abnormal neurophysiology | larval stage, with Scer\GAL4^Toll-6-D42

Phenotype manifest in

Allele

bouton | heat sensitive

Frq1^hs.PP

embryonic/larval neuromuscular junction, with Scer\GAL4^Toll-6-D42

motor neuron | heat sensitive

Frq1^hs.PP

NMJ bouton, with Scer\GAL4^elav-C155

Frq1^UAS.cRPa

type I bouton, with Scer\GAL4^Toll-6-D42

Frq1^UAS.cRPa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (44)

Hsap\NCS1

12 of 14

Yes

6 of 14

6 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

Hsap\LOC118142757

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (41)

Rnor\Ncs1

12 of 14

Yes

6 of 14

6 of 14

6 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (44)

Mmus\Ncs1

11 of 14

Yes

6 of 14

6 of 14

6 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm21941

1 of 14

Yes

Mmus\Gm40853

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (38)

Xtro\ncs1

10 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (56)

Drer\ncs1b

12 of 14

Yes

Drer\ncs1a

10 of 14

Yes

6 of 14

6 of 14

6 of 14

6 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

Drer\si:ch211-245j22.3

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Drer\si:ch211-103a14.5

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (21)

Cele\ncs-1

14 of 14

Yes

7 of 14

6 of 14

5 of 14

5 of 14

Yes

Cele\calm-1

2 of 14

Cele\ncs-6

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (24)

Agam\AgaP_AGAP013126

6 of 12

Yes

5 of 12

5 of 12

4 of 12

4 of 12

4 of 12

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (46)

Atha\SOS3

6 of 13

Yes

Atha\CBL5

5 of 13

Atha\CBL8

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

Yes

Atha\AT1G05990

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\FRQ1

11 of 13

Yes

Scer\CNB1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ncs1

8 of 12

Yes

Spom\cam1

1 of 12

Spom\SPCC830.06

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Frq1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (31)

Frq2

13 of 13

Nca

11 of 13

10 of 13

8 of 13

7 of 13

5 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

Cam

1 of 13

CanB2

1 of 13

CanB

1 of 13

CBP

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

elm

1 of 13

myd

1 of 13

Nox

1 of 13

scf

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NCS1; neuronal calcium sensor 1

12 of 14

603315

HPCA; hippocalcin

6 of 14

142622

DYSTONIA 2, TORSION, AUTOSOMAL RECESSIVE; DYT2

torsion dystonia 2

HPCAL1; hippocalcin like 1

6 of 14

600207

GUCA1B; guanylate cyclase activator 1B

5 of 14

602275

RETINITIS PIGMENTOSA 48; RP48

retinitis pigmentosa 48

HPCAL4; hippocalcin like 4

5 of 14

619211

RCVRN; recoverin

5 of 14

179618

VSNL1; visinin like 1

5 of 14

600817

GUCA1C; guanylate cyclase activator 1C

4 of 14

605128

KCNIP1; potassium voltage-gated channel interacting protein 1

4 of 14

604660

KCNIP2; potassium voltage-gated channel interacting protein 2

4 of 14

604661

KCNIP3; potassium voltage-gated channel interacting protein 3

4 of 14

604662

KCNIP4; potassium voltage-gated channel interacting protein 4

4 of 14

608182

NCALD; neurocalcin delta

4 of 14

606722

CLXN; calaxin

3 of 14

619564

CILIARY DYSKINESIA, PRIMARY, 53; CILD53

GUCA1A; guanylate cyclase activator 1A

3 of 14

600364

CONE DYSTROPHY 3; COD3

cone-rod dystrophy 14

Hsap\LOC118142757

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

In contrast to Frq2, does not interact with ric8a.

(UniProt, P37236 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59117

DroID - A comprehensive database of gene and protein interactions.

FBgn0030897

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-59

Cytogenetic map

16F7-17A1

Sequence location

X:18,155,369..18,174,744 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

16F7-17A1

Limits computationally determined from genome sequence between P{EP}ari-1^EP317 and P{EP}EP1378

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

16F1-16F3

(Pongs, 1993.9.29)

Experimentally Determined Recombination Data

Location

1-59

(FlyBase, 2016)

1-59.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (45)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

39197

w¹¹¹⁸; P{GMR58H01-GAL4}attP2

49042

w¹¹¹⁸; P{GMR23G09-GAL4}attP2

75788

w¹¹¹⁸; P{R54E11-p65.AD}attP40

Korea

12008

P{VT063635-GAL4}attP2

12009

P{VT063636-GAL4}attP2

14906

P{VT063630-GAL4}attP2

15337

P{VT063627-GAL4}attP2

15588

P{VT063633-GAL4}attP2

16665

P{VT063636-lexA::GADfl}attP40

16687

P{VT063635-lexA::GADfl}attP40

VDRC

v23428

w¹¹¹⁸; P{GD13502}v23428

v23426

w¹¹¹⁸; P{GD13502}v23426/TM3

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (11)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030897

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030897

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Pongs et al., 1993)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Flies overexpressing the Frq1 product show the same electrophysiological defect as those lacking Frq1 activity, suggesting that Frq1 is involved in modulating synaptic facilitation.

(Rivosecchi et al., 1994)

Frq1 encodes a novel Ca²⁺ binding protein, in vitro Ca²⁺ binds to both the third and fourth EF-hands. Frq1 is expressed in the central and peripheral nervous system of embryos, larvae and adults, and is particularly enriched at the synapses. Overexpression of Frq1 at the neuromuscular junction causes enhanced frequency-dependent facilitation of neurotransmitter release.

(Pongs et al., 1993)

Examination of mutant Frq1 action potentials at motor terminals suggest that Frq1 is involved in the control of neural excitability.

(Mallart et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

The genes Frq2 and Frq1 share >70% identity and close proximity, and so may have been derived from a DNA-based tandem duplication.

(Langille and Clark, 2007)

Nomenclature History

Source for database identify of

Source for identity of: Frq1 CG5744

(Ferrus, 2005.4.22)

Nomenclature comments

Etymology

In view of the electrophysiological T(1;Y)V7 phenotype, a frequency-dependent facilitation of neurotransmitter release at neuromuscular junctions, the product of the gene affected by the rearrangement is referred to as "Frequenin".

(Pongs et al., 1993)

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

Name Synonyms

Frequenin

(Beckingham, 1995)

Frequenin 1

(Felix et al., 2012, Anaka et al., 2008, Romero-Pozuelo et al., 2007)

Frequenin2

(Kollmar, 2006)

Frequentin

(Dason et al., 2009)

Frequinin

(Pongs et al., 1991)

frequenin

(Diao et al., 2009, Cremona and De Camilli, 2001, Braunewell and Gundelfinger, 1999, Posey et al., 1997, Pongs, 1993.9.29)

frequenin 1

(Tessier and Broadie, 2011)

Secondary FlyBase IDs

FBgn0004120

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32797

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P37236

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P37236

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PHW6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P37236

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030897

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32797/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030897

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030897

FlyMine - An integrated database for Drosophila genomics

FBgn0030897

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5744

MARRVEL_MODEL - MARRVEL (model organism gene)

32797

Linkouts

BioGRID - A database of protein and genetic interactions.

59117

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030897

DroID - A comprehensive database of gene and protein interactions.

FBgn0030897

DRSC - Results frm RNAi screens

FBgn0030897

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Frq1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030897

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030897

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5744

Flygut - An atlas of the Drosophila adult midgut

FBgn0030897

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003144

References (64)

The following external sites may use different assemblies or annotations than FlyBase.

-0.70

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