FlyBase Gene Report: Dmel\CCKLR-17D3

General Information

Symbol

Dmel\CCKLR-17D3

Species

D. melanogaster

Name

Cholecystokinin-like receptor at 17D3

Annotation Symbol

CG32540

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030954

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Cholecystokinin-like receptor at 17D3 (CCKLR-17D3) encodes a class A transmembrane G protein coupled receptor that binds the neuropeptide ligand encoded by Dsk. It acts through the cAMP-PKA-CREB pathway and it is needed for the growth of neuromuscular junctions. It regulates food ingestion, satiety, hyperactivity, aggression and escape-related locomotion. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DSK-R1, CCK-like receptor at 17D3

Key Links

Genomic Location

Cytogenetic map

17D3-17D4

Sequence location

X:18,764,483..18,774,027 [-]

Recombination map

1-60

RefSeq locus

NC_004354 REGION:18764483..18774027

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables G protein-coupled receptor activity

inferred from sequence or structural similarity

(Radford et al., 2002, Brody and Cravchik, 2000)

enables gastrin receptor activity

inferred from electronic annotation with InterPro:IPR000314

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables neuropeptide receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001201778

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence model

(Hewes and Taghert, 2001)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in adult locomotory behavior

inferred from mutant phenotype

(Kiss et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from sequence model

(Hewes and Taghert, 2001)

involved_in neuropeptide signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001201778

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from sequence model

(Hewes and Taghert, 2001)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001201778

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS -: The majority of neuropeptide/protein hormone GPCR belong to the Class A family of GPCRs. The secreted ligands for this subfamily include neuropeptides, peptide hormones and protein hormones. (Adapted from FBrf0195077).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CCKLR-17D3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7M3J6)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

RNAseq data also supports the deletion of CCKLR-17D3-RA; the transcript model supported by cDNAs fails to correspond to either exon junctions or RPKM values.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CCKLR-17D3-RB

FBtr0113003

NM_001103553

2230

584

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CCKLR-17D3-PB

FBpp0111916

62.2

584

8.09

NP_001097023

AAF48879

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q7M3J6

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CCKLR-17D3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CCKLR-17D3_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CCKLR-17D3 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CCKLR-17D3

View exonic expression by tissue for Dmel\CCKLR-17D3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32864/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030954

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030954

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030954

Flygut - An atlas of the Drosophila adult midgut

FBgn0030954

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CCKLR-17D3

Transgenic constructs containing regulatory region of CCKLR-17D3

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel7464

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Act5C.PU

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

CCKLR-17D3^GD3431

Other Phenotypes

Allele

abnormal behavior | adult stage

CCKLR-17D3^KK110484

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CCKLR-17D3^GD731

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CCKAR

11 of 15

Yes

Hsap\CCKBR

10 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cckar

10 of 15

Yes

Mmus\Cckbr

10 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Cckar

10 of 13

Yes

Rnor\Cckbr

9 of 13

Xenopus tropicalis (Western clawed frog) (2)

Xtro\cckar

7 of 12

Yes

Xtro\LOC101732125

2 of 12

Yes

Danio rerio (Zebrafish) (3)

Drer\cckar

11 of 15

Yes

Drer\LOC100331465

11 of 15

Yes

Drer\si:dkey-1h24.2

10 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\ckr-2

12 of 15

Yes

Cele\ckr-1

10 of 15

Yes

Cele\C34B4.3

1 of 15

Yes

Cele\gnrr-7

1 of 15

Cele\R13H4.7

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (10)

Gene Symbol

Score

Source

Alignment

7 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

anxiety modulator(s), CCK-like receptors

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CCKAR; cholecystokinin A receptor

11 of 15

118444

CCKBR; cholecystokinin B receptor

10 of 15

118445

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0030954

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Neuroactive ligand-receptor interaction

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-60

Cytogenetic map

17D3-17D4

Sequence location

X:18,764,483..18,774,027 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

17D3-17D4

Limits computationally determined from genome sequence between P{EP}EP443^EP443&P{EP}Bx^EP1383 and P{EP}Pk17E^EP438

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-60

(FlyBase, 2016)

1-59.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

84463

w^* TI{TI}CCKLR-17D3^attP

84365

w^* TI{2A-lexA::p65}CCKLR-17D3^2A-lexA

56619

y¹ w^* Mi{MIC}CCKLR-17D3^MI11560

28333

y¹ v¹; P{TRiP.JF02968}attP2/TM3, Sb¹

13429

C(1;Y)1, y¹ P{SUPor-P}CCKLR-17D3^KG01373; ry⁵⁰⁶

67866

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05684}attP40

VDRC

v102039

P{KK110484}VIE-260B

v1815

w¹¹¹⁸; P{GD731}v1815

v9154

w¹¹¹⁸; P{GD3431}v9154/TM3

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP03677

Other clones

IP03777

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030954

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030954

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32864

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: CCKLR-17D3 CG6894

(McBride, 2001.3.16)

Source for database merge of

Source for merge of: CCKLR-17D3 anon-WO0170980.64 anon-WO0170980.65

(FlyBase, 1996-)

Source for merge of: CCKLR-17D3 CG6881

(McBride, 2001.3.16)

Additional comments

Annotations CG6881, CG6894 merged as CG32540 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CCKLR-17D3 anon-WO0170980.64 anon-WO0170980.65 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32864

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7M3J6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7M3J6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7M3J6

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030954

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32864/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030954

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CCKLR-17D3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030954

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030954

Flygut - An atlas of the Drosophila adult midgut

FBgn0030954

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32864

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008438

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG32540

MARRVEL_MODEL - MARRVEL (model organism gene)

32864

modMine - A data warehouse for the modENCODE project

FBgn0030954

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0030954

DRSC - Results frm RNAi screens

FBgn0030954

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030954

FlyMine - An integrated database for Drosophila genomics

FBgn0030954

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Neuroactive ligand-receptor interaction

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CCKLR-17D3

(Liu et al., 2021, Gao et al., 2020, Kondo et al., 2020, Wu et al., 2020, Yanagawa et al., 2020, Meltzer et al., 2019, Rao and Deng, 2019.10.23, Crocker et al., 2016, Mohammad et al., 2016, Kiss et al., 2013, Caers et al., 2012, Chen and Ganetzky, 2012, Japanese National Institute of Genetics, 2012.5.21)

CCKLR-D3

(Walker et al., 2013)

CG32540

(Kondo et al., 2020, Kiss et al., 2013, Keleman et al., 2009.8.5, Perkins et al., 2009, Metpally and Sowdhamini, 2005)

CG6861

(Vanden Broeck, 2001)

CG6881

(Hauser et al., 2006, Hauser et al., 2006, Kubiak et al., 2002, Hewes and Taghert, 2001, Hewes and Taghert, 2001.7.31)

CG6894

(Kubiak et al., 2002, Hewes and Taghert, 2001, Vanden Broeck, 2001)

DSK-R1

(Bass et al., 2014, Kubiak et al., 2002)