FlyBase Gene Report: Dmel\SdhBL

MITOCHONDRIAL COMPLEX II (SUCCINATE DEHYDROGENASE) - CATALYTIC SUBUNITS -: Succinate dehydrogenase (SDH, OXPHOS Complex II) connects the essential metabolic OXPHOS and tricarboxylic acid (TCA) pathways in eukaryotic cells by catalyzing the oxidation of succinic acid to fumaric acid in the TCA cycle, which is coupled to the reduction of flavin-adenine dinucleotide (FAD) to produce FADH2 and transfer electrons to ubiquinone in the electron transport chain. SDH is composed of only four subunits: two hydrophilic proteins (SDHA and SDHB) and two transmembrane proteins (SDHC and SDHD), together with the prosthetic groups devoted to electron transfer. SDHA and SDHB are covalently bound by FAD and three Fe-S clusters to form a catalytic heterodimer. In addition, the C-terminal domain of SDHB links the SDHA-SDHB subunit to the SDHC-SDHD subunit. (Adapted from PMID:37490987.)
MITOCHONDRIAL COMPLEX II (SUCCINATE DEHYDROGENASE) - TESTIS-SPECIFIC VARIANT -: Mitochondrial complex II/Succinate Dehydrogenase is a heterotetrameric protein complex anchored to the inner mitochondrial membrane via two integral membrane subunits. It functions both in the tricarboxylic acid (TCA) cycle and the electron transport chain (ETC) by catalysing the oxidation of succinate to fumarate and then, via electron transfer, reducing ubiquinone to ubiquinol. (Adapted from PMID:24954416.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\SdhBL for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VWN3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

SdhBL-RA

FBtr0074648

NM_133112

1888

437

SdhBL-RC

FBtr0344973

NM_001298491

1949

437

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

SdhBL-PA

FBpp0074419

46.5

437

10.02

FBpp0311226

46.5

437

10.02

Q9VWN3

NP_001285420

AHN59890

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

437 aa isoforms: SdhBL-PA, SdhBL-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SdhBL using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.59

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\SdhBL in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\SdhBL

View exonic expression by tissue for Dmel\SdhBL

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030975

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32887/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030975

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030975

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030975

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7349

Flygut - An atlas of the Drosophila adult midgut

FBgn0030975

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of SdhBL

Transgenic constructs containing regulatory region of SdhBL

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel7465

(Ryder, 2004.4.26)

Inferred from location ( 21 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

SdhBL^GD1574

viable, with Scer\GAL4^tin.CΔ4

SdhBL^GD1574

Sterility

Allele

male fertile, with Scer\GAL4^nanos.PG

SdhBL^HMJ22015

Phenotype manifest in

Allele

larval multidendritic neuron, with Scer\GAL4^109(2)80

SdhBL^HMJ22015

mitochondrion | larval stage, with Scer\GAL4^109(2)80

SdhBL^HMJ22015

neuronal cell body | larval stage, with Scer\GAL4^109(2)80

SdhBL^HMJ22015

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\SDHB

8 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Sdhb

7 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Sdhb

6 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\sdhb

4 of 13

Yes

Danio rerio (Zebrafish) (1)

Drer\sdhb

7 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\sdhb-1

10 of 14

Yes

Cele\F42A8.1

1 of 14

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP007309

5 of 12

Yes

Arabidopsis thaliana (thale-cress) (3)

Atha\SDH2-1

10 of 13

Yes

Atha\SDH2-2

10 of 13

Yes

Atha\SDH2-3

8 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\SDH2

9 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sdh2

8 of 12

Yes

Escherichia coli (enterobacterium) (3)

Ecol\sdhB

8 of 11

Yes

Ecol\frdB

4 of 11

Yes

Ecol\glcF

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:SdhBL. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

SdhB

10 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SDHB; succinate dehydrogenase complex iron sulfur subunit B

model of paraganglioma

IEA

(FlyBase, 2019-)

model of Carney-Stratakis syndrome

IEA

(FlyBase, 2019-)

model of gastrointestinal stromal tumor

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

SdhBL^HMJ22015

ameliorates amyotrophic lateral sclerosis type 1

modeled by Sod1^G85R

(Nemtsova et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SDHB; succinate dehydrogenase complex iron sulfur subunit B

8 of 14

185470

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Sod1

suppressible

SdhBL

(Nemtsova et al., 2023)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59195

DroID - A comprehensive database of gene and protein interactions.

FBgn0030975

MIST (protein-protein) - An integrated Molecular Interaction Database

32887

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-61

Cytogenetic map

17F2-17F2

Sequence location

X:18,894,446..18,896,581 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

17F2-17F2

Limits computationally determined from genome sequence between P{EP}CG7326^EP1133 and P{EP}CG7502^EP1326

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-61

(FlyBase, 2016)

1-60.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

15192

y¹ P{SUPor-P}SdhBL^KG09502

58100

y¹ v¹; P{TRiP.HMJ22015}attP40/CyO

43627

y¹ w^* Mi{MIC}SdhBL^MI07299

VDRC

v110007

P{KK115797}VIE-260B

v51482

w¹¹¹⁸; P{GD1574}v51482

Genomic Clones (6)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE03249

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030975

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030975

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: SdhBL CG7349

(Porcelli and Marygold, 2014.9.16)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG7349

(Curcio et al., 2024, Mandilaras and Missirlis, 2012, Ni et al., 2010.12.1, Wasbrough et al., 2010, Keleman et al., 2009.8.5, Caggese et al., 2006, Dorus et al., 2006, Tripoli et al., 2005)

DHSB

(Caggese et al., 2006)

SdhBL

(François et al., 2023, Nemtsova et al., 2023, Porcelli and Marygold, 2014.9.16)

SdhLB

(Curcio et al., 2024)

Name Synonyms

Ferredoxin

(Mandilaras and Missirlis, 2012)

Succinate dehydrogenase Iron-sulfur protein

(Tripoli et al., 2005)

Succinate dehydrogenase [ubiquinone] iron-sulfur protein

(Caggese et al., 2006)

Succinate dehydrogenase, subunit B (iron-sulfur)-like

(Porcelli and Marygold, 2014.9.16)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 57 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32887

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VWN3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VWN3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0030975

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32887/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030975

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030975

FlyMine - An integrated database for Drosophila genomics

FBgn0030975

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7349

MARRVEL_MODEL - MARRVEL (model organism gene)

32887

Linkouts

BioGRID - A database of protein and genetic interactions.

59195

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030975

DroID - A comprehensive database of gene and protein interactions.

FBgn0030975

DRSC - Results frm RNAi screens

FBgn0030975

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030975

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030975

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7349

Flygut - An atlas of the Drosophila adult midgut

FBgn0030975

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

32887

References (38)

The following external sites may use different assemblies or annotations than FlyBase.

3.59

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