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Gene Dmel\RhoGAP18B

General Information
Symbol	Dmel\RhoGAP18B	Species	D. melanogaster
Name	RhoGAP18B	Annotation symbol	CG7481
Feature type	protein_coding_gene	FlyBase ID	FBgn0030986
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	18A3-18A4	Sequence location	X:19,037,484..19,059,362 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol RhoGAP18B (CG7481, FBgn0030986). It has the cytological map location 18A3-18A4. Its sequence location is X:19037484..19059362. Its molecular function is described as GTPase activator activity. It is involved in the biological processes: actin filament organization; signal transduction. 21 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 18A3-18A4   Limits computationally determined from genome sequence between P{EP}CG7326EP1133 and P{EP}CG7502EP1326
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\RhoGAP18B for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RhoGAP18B-RA FBtr0074699  NM_133123   2192   468
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein RhoGAP18B-PA   FBpp0074468   52.2   468   8.27     NP_573351   AAF48919
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0030986
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RhoGAP (IPR000198) Rho GTPase activation protein (IPR008936)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA439889   AA539040   AA941531   AE003511 AAF48919   AI293719   AI531523   AI531524   AI532572   AI542316   AW942810   AY061332 AAL28880   BI163520   BI369973   BI637008   BI640502   BP551468   CO154748   CO156160   CO275535   CO276233   CO290993   CO301827   CO307956   CO313100   CO313538   CO319596   CO321322   CO329965   CO333294   CZ471960   CZ472704   CZ474428   CZ475841   CZ476451   CZ476452   CZ476755   CZ476869   CZ480148   CZ480149   CZ480168   CZ483668   CZ486329   CZ486330   CZ488739   DQ486141 ABF50837   DQ486142 ABF50838   DQ486143 ABF50839   EC065156   EC067417   EC072176   EC085486   EC085533   EC089911   EC195212   EC205357   EC209516   EC210495   EC214037   EC238133   EC242346   EC254052   EC258003   EC265763
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VWL9 Q00IN0 Q00IM9
Maps to
Does NOT map to
Identified with	LD25711 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 3358
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA941531
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030986 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0030986 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0030986
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 17 )
For All Classical Alleles Show
Allele of RhoGAP18B Class Mutagen Stocks Known lesion RhoGAP18BEP1326 P-element activity 1 Yes RhoGAP18BEP1439 P-element activity 0 Yes RhoGAP18BEP1621 P-element activity 0 Yes RhoGAP18BGS9097 0 -- RhoGAP18BKG00160 P-element activity 1 Yes RhoGAP18Bd01002 P-element activity 1 -- RhoGAP18Bd02256 P-element activity 0 -- RhoGAP18Bd05271 P-element activity 1 -- RhoGAP18Bd07621 P-element activity 0 -- RhoGAP18Bd08872 P-element activity 1 -- RhoGAP18Bd09463 P-element activity 0 -- RhoGAP18Bd09663 P-element activity 0 -- RhoGAP18Be02324 piggyBac transposase 1 -- RhoGAP18Be02346 piggyBac transposase 1 -- RhoGAP18Bf01190 piggyBac transposase 1 -- RhoGAP18Bf03742 piggyBac transposase 1 -- RhoGAP18Bf06094 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RhoGAP18B Class Mutagen Stocks Known lesion RhoGAP18BGD12250 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes RhoGAP18BGD12266 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes RhoGAP18BGD15353 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes RhoGAP18BdsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 2 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)22622DL
Useful duplication	Dp(1;1)BSTAG
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12250} NA P{GD12266} NA P{GD15353} NA P{UAS-RhoGAP18B-dsRNA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}CG7502EP1326 NA P{EP}CG7502EP1439 NA P{EP}CG7502EP1621 NA P{GSV6}CG7502GS9097 NA P{XP}RhoGAP18Bd01002 NA P{XP}RhoGAP18Bd02256 NA P{XP}RhoGAP18Bd05271 NA P{XP}RhoGAP18Bd07621 NA P{XP}RhoGAP18Bd08872 NA P{XP}RhoGAP18Bd09463 NA P{XP}RhoGAP18Bd09663 NA miscellaneous insertions PBac{RB}RhoGAP18Be02324 NA PBac{RB}RhoGAP18Be02346 NA PBac{WH}RhoGAP18Bf01190 NA PBac{WH}RhoGAP18Bf03742 NA PBac{WH}RhoGAP18Bf06094 NA P{SUPor-P}CG7502KG00160 NA
Related Comments
	Please look at the allele reports for the complete phenotype data RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: increased or polarized (uneven) accumulation of F-actin. (Kiger et al., 2003)
Gene Ontology: Function, Process & Cellular Component ( 4 )
Molecular Function
CV term Evidence References
GTPase activator activity inferred from sequence or structural similarity with NCBI_NP:064656 (FlyBase Genome Annotators, 2002-2003)
Biological Process
CV term Evidence References
actin filament organization inferred from mutant phenotype (Kiger et al., 2003)
signal transduction inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RhoGAP18B allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 59205 Drosophila PIMRider - The Drosophila Protein Interaction map • 522259
Orthologs
Genome-wide drosophilid orthologs	Dpse\GA22300 Dper\GL14655
Curated drosophilid orthologs	Dpse\GA20383
Linkouts	InParanoid orthologs - Eukaryotic orthologs • SPAC26A3.09c Schizosaccharomyces pombe (Sanger) WBGene00040955 Caenorhabditis briggsae (Wormbase) ENSXETP00000025496 Xenopus tropicalis (Ensembl) ENSRNOP00000043804 Rattus norvegicus (Ensembl)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 20 )
Szeged	- P{EP}CG7502EP1326 - P{EP}CG7502EP1621 - P{EP}CG7502EP1439
Harvard	- P{XP}RhoGAP18Bd01002 - P{XP}RhoGAP18Bd05271 - P{XP}RhoGAP18Bd08872 - PBac{RB}RhoGAP18Be02324 - PBac{RB}RhoGAP18Be02346 - PBac{WH}RhoGAP18Bf01190 - PBac{WH}RhoGAP18Bf03742 - PBac{WH}RhoGAP18Bf06094
VDRC	v28039 w1118; P{GD12250}v28039/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR49A05
cDNA Clones ( 24 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD25711
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD08603 SD02606 LD18742 SD18884 RE02940 SD02607 SD23384 SD04127 LP06920 LD14570 RE56328
Other clones	EK182252 EK117847 EN04723 EK290810 EK279264 EK306420 EK075738 EK294249 EK256257 EK231354 EN07449 EK120108
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0030986 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0030986
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG7481 CG7531 Source for merge of CG7481 CG7531 was a shared cDNA (date:010720). (FlyBase, 1996-) Source for merge of: RhoGAP18B CG17015 Annotations CG7481, CG17015, CG7531 merged as CG7481 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_133123 RefSeq (Proteins) • NP_573351 DDBJ / EMBL / GenBank • AA941531 AE003511 AAF48919 AW942810 AY061332 AAL28880 CZ471960 CZ472704 CZ474428 CZ475841 CZ476451 CZ476452 CZ476755 CZ476869 CZ480148 CZ480149 CZ480168 CZ483668 CZ486329 CZ486330 CZ488739 ABF50837 DQ486141 ABF50838 DQ486142 ABF50839 DQ486143 UniProtKB/TrEMBL • Q9VWL9 Q00IN0 Q00IM9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RhoGAP (IPR000198) Rho GTPase activation protein (IPR008936)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 59205 DEDB - Drosophila exon database: splicing graphs • 3358 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0030986 Drosophila PIMRider - The Drosophila Protein Interaction map • 522259 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0030986 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0030986 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0030986 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0030986 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0030986 InParanoid orthologs - Eukaryotic orthologs • SPAC26A3.09c Schizosaccharomyces pombe (Sanger) WBGene00040955 Caenorhabditis briggsae (Wormbase)