FlyBase Gene Report: Dmel\Arp10

General Information

Symbol

Dmel\Arp10

Species

D. melanogaster

Name

Actin-related protein 10

Annotation Symbol

CG12235

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031050

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Actin-related protein 10 (Arp10) encodes a component of the dynactin multiprotein complex that is involved in microtubule-based movement. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Arp11

Key Links

Genomic Location

Cytogenetic map

18D12-18D12

Sequence location

X:19,644,531..19,646,176 [-]

Recombination map

1-63

RefSeq locus

NC_004354 REGION:19644531..19646176

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from direct assay

(Eckley and Schroer, 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in retrograde axonal transport of mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000232947

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of dynactin complex

inferred from biological aspect of ancestor with PANTHER:PTN000232945

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with MGI:MGI:1891654

(Eckley and Schroer, 2003)

Gene Group (FlyBase)

ACTIN-RELATED PROTEINS

DYNACTIN COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Actin-related protein 10 (Arp10) encodes a component of the dynactin multiprotein complex that is involved in microtubule-based movement. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

ACTIN-RELATED PROTEINS -: Actin-related proteins (Arp) share significant sequence identity with actins. Arps broadly fall into two groups: nuclear Arps, involved in chromatin remodeling; and cytoplasmic Arps, associated with actin. (Adapted from PMID:21859859).
DYNACTIN COMPLEX -: Dynactin is a large 1.2 MDa multisubunit protein complex that associates with the cytoplasmic dynein complex to drive microtubule-based transport. (Adapted from PMID:15473859 and PMID:25751425).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Arp10 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VWE8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Arp10-RA

FBtr0074760

NM_134494

1374

378

Arp10-RB

FBtr0345991

NM_001298523

1536

378

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Arp10-PA

FBpp0074529

42.2

378

4.95

FBpp0311876

42.2

378

4.95

Q9VWE8

NP_001285452

AHN59922

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

378 aa isoforms: Arp10-PA, Arp10-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VWE8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Arp10 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Arp10_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.45

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Arp10 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Arp10

View exonic expression by tissue for Dmel\Arp10

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32969/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031050

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031050

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031050

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12235

Flygut - An atlas of the Drosophila adult midgut

FBgn0031050

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Arp10

Transgenic constructs containing regulatory region of Arp10

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 15 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Arp10^GD7542

lethal | recessive

Arp10^PL101

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Arp10^GD7542

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Arp10^GD7542

viable, with Scer\GAL4^Mef2.PR

Arp10^GD7542

viable, with Scer\GAL4^tin.CΔ4

Arp10^GD7542

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Arp10^GD7542

visible, with Scer\GAL4^pnr-MD237

Arp10^GD7542

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

Arp10^GD7542

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (39)

Hsap\ACTR10

13 of 14

Yes

Hsap\ACTL7A

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Actr10

13 of 14

Yes

Rnor\Actl7a

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (26)

Mmus\Actr10

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\actr10

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (20)

Drer\actr10

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-241j12.3

1 of 14

Drer\zgc:101810

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\arp-11

11 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP012077

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (19)

Atha\ACT1

2 of 13

Yes

Atha\ACT2

2 of 13

Yes

Atha\ACT3

2 of 13

Yes

Atha\ACT4

2 of 13

Yes

Atha\ACT7

2 of 13

Yes

Atha\ACT8

2 of 13

Yes

Atha\ACT9

2 of 13

Yes

Atha\ACT11

2 of 13

Yes

Atha\ACT12

2 of 13

Yes

Atha\ARP2

2 of 13

Yes

Atha\ARP4

2 of 13

Yes

Atha\AT2G42100

2 of 13

Yes

Atha\AT2G42170

2 of 13

Yes

Atha\DIS1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\ARP10

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\act1

2 of 12

Yes

Spom\arp1

2 of 12

Yes

Spom\arp42

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Arp10. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ACTL7A; actin like 7A

model of spermatogenic failure 86

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Arp10^GD7542

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ACTR10; actin related protein 10

13 of 14

619731

ACTL7A; actin like 7A

3 of 14

604303

SPERMATOGENIC FAILURE 86; SPGF86

spermatogenic failure 86

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Arp10 - Arp1

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - CG3548

two hybrid

(Hu et al., 2017.6.13)

Arp10 - DCTN1-p150

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - DCTN2-p50

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - DCTN3-p24

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - DCTN4-p62

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - DCTN5-p25

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - DCTN6-p27

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - LRR

experimental knowledge based

(Guruharsha et al., 2011)

Arp10 - cpb

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59265

DroID - A comprehensive database of gene and protein interactions.

FBgn0031050

MIST (protein-protein) - An integrated Molecular Interaction Database

32969

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VWE8

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-63

Cytogenetic map

18D12-18D12

Sequence location

X:19,644,531..19,646,176 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

18D12-18D12

Limits computationally determined from genome sequence between P{EP}Sec61γ^EP1511 and P{EP}EP1116&P{EP}EP1344^EP1344

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-63

(FlyBase, 2016)

1-62.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

15263

y¹ P{SUPor-P}Arp10^KG10414

64570

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05589}attP40

12235R-1

v104010

v31711

w¹¹¹⁸; P{GD7542}v31711

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (94)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD33759

Other clones

DPiM_LD33759

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031050

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031050

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Arp11 CG12235

(Goldstein and Gunawardena, 2000)

Source for identity of: Arp10 Arp11

(FlyBase, 2009-)

Nomenclature comments

'Arp11' renamed to 'Arp10' to rationalize the nomenclature of Actin-related protein (Arp) genes (by orthology with the mammalian and yeast genes). This gene was originally referred to as 'ARP11' in FBrf0132100, but there is no yeast or mammalian gene with this symbol. However, 'Arp11' is a synonym for the human 'actin-related protein 10 homolog' gene, and the computed vertebrate orthologs of the D. melanogaster gene (CG12235) are all 'ARP10' genes.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Name Synonyms

Actin related protein 11

(Kollmar, 2015.9.15)

Actin-related protein 10

Arp11

(Cammarato et al., 2011)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32969

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VWE8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VWE8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VWE8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32969/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031050

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031050

FlyMine - An integrated database for Drosophila genomics

FBgn0031050

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG12235

MARRVEL_MODEL - MARRVEL (model organism gene)

32969

Linkouts

BioGRID - A database of protein and genetic interactions.

59265

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031050

DroID - A comprehensive database of gene and protein interactions.

FBgn0031050

DRSC - Results frm RNAi screens

FBgn0031050

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Arp10_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031050

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031050

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12235

Flygut - An atlas of the Drosophila adult midgut

FBgn0031050

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0031050

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008318

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

32969

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VWE8

References (49)

The following external sites may use different assemblies or annotations than FlyBase.

2.45

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