FlyBase Gene Report: Dmel\Rab35

General Information

Symbol

Dmel\Rab35

Species

D. melanogaster

Name

Rab35

Annotation Symbol

CG9575

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031090

Gene Model Status

Stock Availability

15 publicly available

Gene Summary

Rab35 (Rab35) encodes a protein that regulates proximal versus distal growth of seamless tubes in the terminal cells of the tracheal system. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DmRab35, dRab35

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:20,155,766..20,159,872 [-]

Recombination map

1-64

RefSeq locus

NC_004354 REGION:20155766..20159872

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (30 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579, InterPro:IPR005225

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables GTPase activity

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Biological Process (16 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in apical protein localization

inferred from mutant phenotype

(assigned by UniProt )

(Jewett et al., 2017)

involved_in cytoskeletal rearrangement involved in phagocytosis, engulfment

inferred from mutant phenotype

(assigned by UniProt )

(Shim et al., 2010)

involved_in endosome to plasma membrane protein transport

inferred from mutant phenotype

(assigned by UniProt )

(Shim et al., 2010)

involved_in exosomal secretion

inferred from mutant phenotype

(Corrigan et al., 2014)

involved_in lumen formation, open tracheal system

inferred from mutant phenotype

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

involved_in mitotic cytokinesis

inferred from mutant phenotype

(Kouranti et al., 2006)

involved_in plasma membrane to endosome transport

inferred from mutant phenotype

(assigned by UniProt )

(Jewett et al., 2017)

involved_in polarity specification of proximal/distal axis

inferred from mutant phenotype

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

involved_in positive regulation of apical constriction involved in ventral furrow formation

inferred from mutant phenotype

(assigned by UniProt )

(Jewett et al., 2017)

involved_in positive regulation of establishment of protein localization

inferred from mutant phenotype

(assigned by UniProt )

(Jewett et al., 2017)

involved_in positive regulation of filopodium assembly

inferred from mutant phenotype

(assigned by UniProt )

(Shim et al., 2010)

involved_in regulation of embryonic cell shape

inferred from mutant phenotype

(assigned by UniProt )

(Jewett et al., 2017)

involved_in viral entry into host cell

inferred from high throughput mutant phenotype

(Hodgson et al., 2019)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in endocytic recycling

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in Rab protein signal transduction

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

involved_in vesicle-mediated transport

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Cellular Component (12 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

located_in anterior cell cortex

inferred from direct assay

(assigned by UniProt )

(Jewett et al., 2017)

located_in cytoplasmic side of apical plasma membrane

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in endosome to plasma membrane transport vesicle

inferred from direct assay

(assigned by UniProt )

(Shim et al., 2010)

located_in filopodium

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in lamellipodium

inferred from direct assay

(assigned by UniProt )

(Shim et al., 2010)

located_in neuronal cell body

inferred from high throughput direct assay

(Chan et al., 2011)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in posterior cell cortex

inferred from direct assay

(assigned by UniProt )

(Jewett et al., 2017)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in endosome membrane

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001178320

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Rab35 (Rab35) encodes a protein that regulates proximal versus distal growth of seamless tubes in the terminal cells of the tracheal system. [Date last reviewed: 2018-10-04]

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Summary (Interactive Fly)

tissue polarity, an essential component of the contractile process that functions as a membrane ratchet to ensure unidirectional movement of intercalating cells - gastrulation - mesoderm invagination - Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth during tracheal development - Skywalker facilitates endosomal trafficking of synaptic vesicles at NMJ boutons by controlling Rab35 activity

Interactive Fly

Gene Model and Products

Number of Transcripts

4

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Rab35 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0070028

2635

201

FBtr0303258

2024

201

FBtr0333548

2159

190

FBtr0333549

3348

201

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0070027

22.9

201

8.82

FBpp0292350

22.9

201

8.82

FBpp0305728

21.6

190

8.62

FBpp0305729

22.9

201

8.82

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

201 aa isoforms: Rab35-PA, Rab35-PB, Rab35-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab35 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rab35_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in anterior cell cortex

inferred from direct assay

(assigned by UniProt )

(Jewett et al., 2017)

located_in cytoplasmic side of apical plasma membrane

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in cytoplasm

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in endosome to plasma membrane transport vesicle

inferred from direct assay

(assigned by UniProt )

(Shim et al., 2010)

located_in filopodium

inferred from direct assay

(assigned by UniProt )

(Schottenfeld-Roames and Ghabrial, 2012)

located_in lamellipodium

inferred from direct assay

(assigned by UniProt )

(Shim et al., 2010)

located_in neuronal cell body

inferred from high throughput direct assay

(Chan et al., 2011)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

located_in posterior cell cortex

inferred from direct assay

(assigned by UniProt )

(Jewett et al., 2017)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab35-GAL4.ATG(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- P7

neuron of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

Comment: 30-40h APF

(Chan et al., 2011)

neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)

glial cell of second optic chiasma | intense

Comment: 30-40h APF

(Jin et al., 2012)

neuron of adult optic lobe

Comment: 30-40h APF

(Jin et al., 2012)

mushroom body | intense

Comment: 30-40h APF

(Jin et al., 2012)

eye photoreceptor cell

Comment: 30-40h APF

(Jin et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab35 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab35

View exonic expression by tissue for Dmel\Rab35

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031090

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0031090

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9575-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9575

Flygut - An atlas of the Drosophila adult midgut

FBgn0031090

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab35

Transgenic constructs containing regulatory region of Rab35

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

(Yang et al., 2012)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^Rab35.ATG

Rab35^S22N.UAS.YFP

Rab35^EYFP

Other Phenotypes

Allele

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

abnormal neurophysiology, with Scer\GAL4^nSyb.PS

abnormal planar polarity, with Avic\GFP^dsRNA.R.UAS, Scer\GAL4^nub.PK

Rab35^EYFP

abnormal planar polarity, with Avic\GFP^{dsRNA.shRNA.UAS.V20.3}, Scer\GAL4^nub.PK

Rab35^EYFP

Phenotype manifest in

Allele

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

embryonic/larval neuromuscular junction, with Scer\GAL4^nSyb.PS

extracellular exosome, with Scer\GAL4^dsx.KI

Rab35^JF02978

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

Rab35^JF02978

wing hair, with Avic\GFP^dsRNA.R.UAS, Scer\GAL4^nub.PK

Rab35^EYFP

wing hair, with Avic\GFP^{dsRNA.shRNA.UAS.V20.3}, Scer\GAL4^nub.PK

Rab35^EYFP

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

10 of 15

Yes

Yes

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

2 of 15

No

No

1

2 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (19)

12 of 13

Yes

Yes

Rnor\LOC102555167

2 of 13

No

No

2 of 13

No

No

1 of 13

No

Yes

Rnor\LOC100363782

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (16)

12 of 12

Yes

Yes

11 of 12

No

Yes

2 of 12

No

No

Xtro\LOC100135070

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (25)

9 of 15

Yes

Yes

Drer\si:dkey-16l2.16

9 of 15

Yes

Yes

6 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

Drer\zgc:171927

2 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (8)

13 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (9)

2 of 9

Yes

No

2 of 9

Yes

No

2 of 9

Yes

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

Saccharomyces cerevisiae (Brewer's yeast) (3)

1 of 15

Yes

No

1 of 15

Yes

Yes

1 of 15

Yes

No

Schizosaccharomyces pombe (Fission yeast) (2)

1 of 12

Yes

No

1 of 12

Yes

No

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FJO )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00006497

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AZ1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY003585

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08789

Mayetiola destructor

Hessian fly

Mdes\Mdes012030

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013620

Anopheles darlingi

American malaria mosquito

Adar\ADAC005537

Anopheles gambiae

Malaria mosquito

Agam\AGAP003028

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014887

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CMD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA012904

Danaus plexippus

Monarch butterfly

Dple\DPOGS206928-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015460

Heliconius melpomene

Postman butterfly

Hmel\HMEL015461

Apis florea

Little honeybee

Aflo\2923477220

Apis mellifera

Western honey bee

Amel\LOC100577870

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002cdc

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000d57

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012676

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_15416

Pediculus humanus

Human body louse

Phum\PHUM240570

Rhodnius prolixus

Kissing bug

Rpro\RPRC008081

Cimex lectularius

Bed bug

Clec\CLEC008758

Cimex lectularius

Bed bug

Clec\CLEC008980

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003912

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03933

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0FML )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015600

Strigamia maritima

European centipede

Smar\SMAR003012

Ixodes scapularis

Black-legged tick

Isca\ISCW015757

Ixodes scapularis

Black-legged tick

Isca\ISCW003929

Ixodes scapularis

Black-legged tick

Isca\ISCW006071

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70918.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74897.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77572.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g00590

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g00570

Tetranychus urticae

Two-spotted spider mite

Turt\tetur19g02010

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_195992

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_223052

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LA6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100185758

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000016136

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000022390

Ciona intestinalis

Vase tunicate

Cint\LOC100185850

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028851

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Rab35^S22N.UAS.YFP

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB35; RAB35, member RAS oncogene family

10 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

coimmunoprecipitation, quantitative reverse transcription pcr

(Olesnicky et al., 2018)

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014, Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

two hybrid, pull down, peptide massfingerprinting

(Hu et al., 2017.6.13, Gillingham et al., 2014)

pull down, peptide massfingerprinting, western blot

(Miao et al., 2019, Gillingham et al., 2014)

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

(Uytterhoeven et al., 2011)

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Zhang et al., 2009)

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ma et al., 2012)

suppressible

(Kroll et al., 2015)

suppressible

(Uytterhoeven et al., 2011)

suppressible

(Schottenfeld-Roames and Ghabrial, 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031090

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33014

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab35

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-64

Cytogenetic map

Sequence location

X:20,155,766..20,159,872 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19B3-19B3

Limits computationally determined from genome sequence between P{EP}amn^EP1639&P{EP}amn^EP367 and P{EP}CG1702^EP1525

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-64

(FlyBase, 2016)

1-63.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

32635

w^* P{EP}Rab35^G11110

62559

w¹¹¹⁸ TI{TI}Rab35^EYFP

9821

y¹ w^*; P{UASp-YFP.Rab35}15

28342

y¹ v¹; P{TRiP.JF02978}attP2

9818

y¹ w^*; P{UASp-YFP.Rab35.Q67L}11

9819

y¹ w^*; P{UAST-YFP.Rab35.S22N}01

9820

y¹ w^*; P{UAST-YFP.Rab35.S22N}06

9817

y¹ w^*; P{UASp-YFP.Rab35.Q67L}01/TM3, Sb¹

104417

y^* w^* P{GawB}Rab35^NP3216 / FM7c

v330170

P{VSH330170}attP40

v101361

P{KK108660}VIE-260B

v318284

PBac{fTRG00931.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (94)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031090

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031090

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33014

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Shim et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

A large increase in the proportion of binucleated cells is seen following transfection of S2 cells with dsRNA made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against Rab35 that is transfected into S2 treated with Listeria monocytogenes reveals Rab35 to be involved in Listeria monocytogenes entry, intracellular growth, and vacuolar escape.

(Cheng et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: binucleate cells.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33014

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031090

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031090

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rab35_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9575

Flygut - An atlas of the Drosophila adult midgut

FBgn0031090

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33014

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9575

modMine - A data warehouse for the modENCODE project

FBgn0031090

Linkouts

DPiM - Drosophila Protein interaction map

FBgn0031090

DroID - A comprehensive database of gene and protein interactions.

FBgn0031090

DRSC - Results frm RNAi screens

FBgn0031090

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0031090

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9575-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031090

FlyMine - An integrated database for Drosophila genomics

FBgn0031090

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33014

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab35

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG9575

(Meadows, 2017.1.11, Dunst et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Sinka et al., 2008, Derré et al., 2007, Zhang et al., 2007, Cermelli et al., 2006, Colicelli, 2004)

DRab35

(Zhang et al., 2005)

DmRab35

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

Q95RH7

(Rojas et al., 2012)

Rab35

(Loerke and Blankenship, 2020, Martin, 2020, Brantley and Fuller, 2019, Hodgson et al., 2019, Jiang et al., 2019, Johnson and Andrew, 2019, Krämer et al., 2019, Miao et al., 2019, Myat et al., 2019, Bellec et al., 2018, Olesnicky et al., 2018, Beer and Wehman, 2017, Coutinho-Budd et al., 2017, Del Signore et al., 2017, Hu et al., 2017.6.13, Jewett et al., 2017, Caviglia et al., 2016, Meehan et al., 2016, Otani et al., 2016, Sarov et al., 2016, Kroll et al., 2015, White et al., 2015, Corrigan et al., 2014, Fernandes et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014, Beckett et al., 2013, O'Sullivan et al., 2013, Zhang et al., 2013, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Koles et al., 2012, Laflamme et al., 2012, Ma et al., 2012, Schottenfeld-Roames and Ghabrial, 2012, Wang et al., 2012, Yang et al., 2012, Otani et al., 2011, Uytterhoeven et al., 2011, Shim et al., 2010, Tan et al., 2009, Zhang et al., 2009, Sinka et al., 2008, Bakal et al., 2007, Bakal et al., 2007, Derré et al., 2007, Zhang et al., 2007, Zhang et al., 2007, Cermelli et al., 2006, Kouranti et al., 2006, Stroschein-Stevenson et al., 2006)

Rab35 CES

(Kaye et al., 2017)

dRab35

(Carim et al., 2019, Zhang and Scott, 2006)

rab35

(Lammers et al., 2017, Jin et al., 2012, Chan et al., 2011)

Name Synonyms

Rab35

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Rab_Effectors_interaction_map

interactome

Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.

References (100)