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Gene: Dmel\Rab35

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General Information
Symbol
Dmel\Rab35
Species
D. melanogaster
Name
Rab35
Annotation Symbol
CG9575
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031090
Gene Model Status
Current
Stock Availability
15 publicly available
Gene Snapshot
Rab35 (Rab35) encodes a protein that regulates proximal versus distal growth of seamless tubes in the terminal cells of the tracheal system. [Date last reviewed: 2018-10-04]
Other Summaries
Also Known As
DmRab35
Key Links
Genomic Location
Cytogenetic map
19B3-19B3
Sequence location
X:20,155,766..20,159,872 [-]
Recombination map
1-64
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI Genome Data Viewer
UCSC Genome Browser
Ensembl Genome Browser
PopFly Genome Browser
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Protein Domains/Motifs
InterPro
Small GTPase; Small GTP-binding protein domain; P-loop containing nucleoside triphosphate hydrolase
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
GTP binding; GTPase activity
Summaries
Gene Group Membership
RAB GTPASES -
The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).
Interactive Fly
tissue polarity, an essential component of the contractile process that functions as a membrane ratchet to ensure unidirectional movement of intercalating cells - gastrulation - mesoderm invagination - Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth during tracheal development - Skywalker facilitates endosomal trafficking of synaptic vesicles at NMJ boutons by controlling Rab35 activity
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Rab35 or the JBrowse view of Dmel\Rab35 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rab35-RA
FBtr0070028
NM_134529
2635
201
Rab35-RB
FBtr0303258
NM_001201749
2024
201
Rab35-RC
FBtr0333548
NM_001272809
2159
190
Rab35-RD
FBtr0333549
NM_001272810
3348
201
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rab35-PA
FBpp0070027
22.9
201
8.82
NP_608373
AAF45371
Rab35-PB
FBpp0292350
22.9
201
8.82
NP_001188678
ADV37760
Rab35-PC
FBpp0305728
21.6
190
8.62
NP_001259738
AGB95577
Rab35-PD
FBpp0305729
22.9
201
8.82
NP_001259739
AGB95578
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

201 aa isoforms: Rab35-PA, Rab35-PB, Rab35-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab35 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (31 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
GTP binding
inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579, InterPro:IPR005225
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
GTPase activity
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (16 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
apical protein localization
inferred from mutant phenotype
(assigned by UniProt )
(Jewett et al., 2017)
cytoskeletal rearrangement involved in phagocytosis, engulfment
inferred from mutant phenotype
(assigned by UniProt )
(Shim et al., 2010)
endosome to plasma membrane protein transport
inferred from mutant phenotype
(assigned by UniProt )
(Shim et al., 2010)
exosomal secretion
inferred from mutant phenotype
(Corrigan et al., 2014)
lumen formation, open tracheal system
inferred from mutant phenotype
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
mitotic cytokinesis
inferred from mutant phenotype
(Kouranti et al., 2006)
plasma membrane to endosome transport
inferred from mutant phenotype
(assigned by UniProt )
(Jewett et al., 2017)
polarity specification of proximal/distal axis
inferred from mutant phenotype
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
positive regulation of apical constriction involved in ventral furrow formation
inferred from mutant phenotype
(assigned by UniProt )
(Jewett et al., 2017)
positive regulation of establishment of protein localization
inferred from mutant phenotype
(assigned by UniProt )
(Jewett et al., 2017)
positive regulation of filopodium assembly
inferred from mutant phenotype
(assigned by UniProt )
(Shim et al., 2010)
regulation of embryonic cell shape
inferred from mutant phenotype
(assigned by UniProt )
(Jewett et al., 2017)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
endocytic recycling
inferred from biological aspect of ancestor with PANTHER:PTN001178320
(assigned by GO_Central )
(Gaudet et al., 2011)
intracellular protein transport
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
Rab protein signal transduction
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
(Gaudet et al., 2011)
vesicle-mediated transport
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
Cellular Component (13 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
anterior cell cortex
inferred from direct assay
(assigned by UniProt )
(Jewett et al., 2017)
cytoplasm
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
cytoplasmic side of apical plasma membrane
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
endosome to plasma membrane transport vesicle
inferred from direct assay
(assigned by UniProt )
(Shim et al., 2010)
filopodium
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
lamellipodium
inferred from direct assay
(assigned by UniProt )
(Shim et al., 2010)
neuronal cell body
inferred from high throughput direct assay
(Chan et al., 2011)
plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
posterior cell cortex
inferred from direct assay
(assigned by UniProt )
(Jewett et al., 2017)
synapse
inferred from high throughput direct assay
(Chan et al., 2011)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
endosome membrane
inferred from biological aspect of ancestor with PANTHER:PTN001178320
(assigned by GO_Central )
(Gaudet et al., 2011)
Golgi membrane
inferred from biological aspect of ancestor with PANTHER:PTN002375302
(assigned by GO_Central )
(Gaudet et al., 2011)
plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN001178320
(assigned by GO_Central )
(Gaudet et al., 2011)
vesicle
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
(FlyBase Curators, 2008--)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
anterior cell cortex
inferred from direct assay
(assigned by UniProt )
(Jewett et al., 2017)
cytoplasm
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
cytoplasmic side of apical plasma membrane
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
endosome to plasma membrane transport vesicle
inferred from direct assay
(assigned by UniProt )
(Shim et al., 2010)
filopodium
inferred from direct assay
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
lamellipodium
inferred from direct assay
(assigned by UniProt )
(Shim et al., 2010)
neuronal cell body
inferred from high throughput direct assay
(Chan et al., 2011)
plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
posterior cell cortex
inferred from direct assay
(assigned by UniProt )
(Jewett et al., 2017)
synapse
inferred from high throughput direct assay
(Chan et al., 2011)
Expression Deduced from Reporters
Reporter: PBac{I-SceI(FRT.Rab35-GAL4.ATG(loxP.3xP3-RFP))}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
neuron of embryonic/larval brain
(Chan et al., 2011)
glial cell | subset of embryonic/larval brain
(Chan et al., 2011)
embryonic/larval salivary gland | restricted
(Jin et al., 2012, Chan et al., 2011)
neuron | subset of ventral nerve cord
(Chan et al., 2011)
wing disc
(Chan et al., 2011)
eye disc
(Chan et al., 2011)
ventral thoracic disc
(Chan et al., 2011)
neuropil glial sheath
(Jin et al., 2012)
Bolwig organ | restricted
(Jin et al., 2012)
embryonic/larval mushroom body | intense
(Jin et al., 2012)
eye disc | restricted
(Jin et al., 2012)
embryonic/larval optic stalk sheath cell
(Jin et al., 2012)
ventral thoracic disc | restricted
(Jin et al., 2012)
wing disc | restricted
(Jin et al., 2012)
pupal stage P6 -- P7
neuron of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)
mushroom body

Comment: 30-40h APF

(Chan et al., 2011)
neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)
glial cell of second optic chiasma | intense

Comment: 30-40h APF

(Jin et al., 2012)
neuron of adult optic lobe

Comment: 30-40h APF

(Jin et al., 2012)
mushroom body | intense

Comment: 30-40h APF

(Jin et al., 2012)
eye photoreceptor cell

Comment: 30-40h APF

(Jin et al., 2012)
adult stage
neuron of adult brain
(Chan et al., 2011)
adult mushroom body | intense
(Jin et al., 2012, Chan et al., 2011)
adult ensheathing neuropil associated glial cell
(Jin et al., 2012)
adult inner optic chiasma giant glial cell
(Jin et al., 2012)
neuron of adult optic lobe
(Jin et al., 2012)
adult antennal lobe
(Jin et al., 2012)
adult insulin secreting cell of pars intercerebralis | intense
(Jin et al., 2012)
neuron of lamina
(Jin et al., 2012)
ellipsoid body
(Jin et al., 2012)
fan-shaped body
(Jin et al., 2012)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab35 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Allele of Rab35
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Rab35EYFP
1
Yes
Rab35G11110
1
Yes
Rab35NP3216
    1
    Yes
    Other relevant insertions
    Transgenic Constructs ( 12 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Rab35
    Allele of Rab35
    Mutagen
    Associated Transgenic Construct
    Stocks
    Rab35S22N.UAS.YFP
    2
    Rab35Q67L.UASp.YFP
    2
    Rab35UASp.YFP
    1
    Rab35JF02978
    1
    Rab35KK108660
    1
    Rab35fTRG00931.sfGFP-TVPTBF
    1
    Rab35GL01874
    1
    Rab35HMS06057
    1
    Rab35VSH330170
    1
    Rab35GD14251
    0
    Rab35NIG.9575R
    0
    Rab35Q67L.UAS.N.EGFP
    0
    Transgenic constructs containing regulatory region of Rab35
    GAL4 construct
    Name
    Expression Data
    PBac{I-SceI(FRT.Rab35-GAL4.ATG(loxP.3xP3-RFP))}
    Yes
    Deletions and Duplications ( 1 )
    Partially disrupted in
    Df(1)Phf7Δ88
    (Yang et al., 2012)
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (13)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Hsap\RAB35
    12 of 15
    Yes
    Yes
    Hsap\IFT27
    1 of 15
    No
    Yes
    Hsap\RAB1A
    1 of 15
    No
    No
    Hsap\RAB1B
    1 of 15
    No
    No
    Hsap\RAB8A
    1 of 15
    No
    No
    Hsap\RAB8B
    1 of 15
    No
    No
    Hsap\RAB10
    1 of 15
    No
    No
    Hsap\RAB12
    1 of 15
    No
    No
    Hsap\RAB13
    1 of 15
    No
    No
    Hsap\RAB15
    1 of 15
    No
    No
    Hsap\RAB19
    1 of 15
    No
    No
    Hsap\RAB30
    1 of 15
    No
    No
     
    Hsap\RAB43
    1 of 15
    No
    No
     
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (11)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Mmus\Rab35
    14 of 15
    Yes
    Yes
    Mmus\Ift27
    1 of 15
    No
    Yes
    Mmus\Rab1a
    1 of 15
    No
    No
     
    Mmus\Rab1b
    1 of 15
    No
    No
    Mmus\Rab8b
    1 of 15
    No
    No
    Mmus\Rab10
    1 of 15
    No
    No
    Mmus\Rab13
    1 of 15
    No
    No
    Mmus\Rab15
    1 of 15
    No
    No
    Mmus\Rab19
    1 of 15
    No
    No
    Mmus\Rab30
    1 of 15
    No
    No
    Mmus\Rab43
    1 of 15
    No
    No
    Rattus norvegicus (Norway rat) (12)
    Rnor\Rab35
    13 of 13
    Yes
    Yes
    Rnor\Ift27
    1 of 13
    No
    Yes
    Rnor\LOC102555167
    1 of 13
    No
    No
    Rnor\Rab1a
    1 of 13
    No
    No
    Rnor\Rab8a
    1 of 13
    No
    No
    Rnor\Rab8b
    1 of 13
    No
    No
    Rnor\Rab10
    1 of 13
    No
    No
    Rnor\Rab13
    1 of 13
    No
    No
    Rnor\Rab15
    1 of 13
    No
    No
    Rnor\Rab19
    1 of 13
    No
    No
    Rnor\Rab30
    1 of 13
    No
    No
    Rnor\Rab43
    1 of 13
    No
    No
    Xenopus tropicalis (Western clawed frog) (6)
    Xtro\rab35
    12 of 12
    Yes
    Yes
    Xtro\rab35
    9 of 12
    No
    Yes
    Xtro\rab8a
    1 of 12
    No
    No
    Xtro\rab8b
    1 of 12
    No
    No
    Xtro\rab15
    1 of 12
    No
    No
    Xtro\rab30
    1 of 12
    No
    No
    Danio rerio (Zebrafish) (17)
    Drer\rab35b
    10 of 15
    Yes
    Yes
    Drer\si:dkey-16l2.16
    10 of 15
    Yes
    Yes
    Drer\rab35a
    6 of 15
    No
    Yes
    Drer\ift27
    1 of 15
    No
    Yes
    Drer\rab1aa
    1 of 15
    No
    No
    Drer\rab1ab
    1 of 15
    No
    No
    Drer\rab1ba
    1 of 15
    No
    No
    Drer\rab1bb
    1 of 15
    No
    No
    Drer\rab8a
    1 of 15
    No
    No
    Drer\rab8b
    1 of 15
    No
    No
    Drer\rab10
    1 of 15
    No
    No
    Drer\rab12
    1 of 15
    No
    No
    Drer\rab13
    1 of 15
    No
    No
    Drer\rab15
    1 of 15
    No
    No
    Drer\rab30
    1 of 15
    No
    No
    Drer\rab43
    1 of 15
    No
    No
    Drer\si:ch211-237l4.6
    1 of 15
    No
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (7)
    Cele\rab-35
    12 of 15
    Yes
    Yes
    Cele\K02E10.1
    1 of 15
    No
    No
    Cele\rab-1
    1 of 15
    No
    No
    Cele\rab-8
    1 of 15
    No
    No
    Cele\rab-10
    1 of 15
    No
    No
    Cele\rab-19
    1 of 15
    No
    No
    Cele\rab-30
    1 of 15
    No
    No
    Arabidopsis thaliana (thale-cress) (9)
    Atha\AT5G59840
    2 of 9
    Yes
    No
    Atha\ATFP8
    2 of 9
    Yes
    No
    Atha\RAB8
    2 of 9
    Yes
    No
    Atha\RAB8C
    2 of 9
    Yes
    No
    Atha\RABE1c
    2 of 9
    Yes
    No
    Atha\RABE1e
    2 of 9
    Yes
    No
    Atha\RA-5
    1 of 9
    No
    No
    Atha\RAB1A
    1 of 9
    No
    No
    Atha\RAB1C
    1 of 9
    No
    No
    Saccharomyces cerevisiae (Brewer's yeast) (3)
    Scer\YPT1
    2 of 15
    Yes
    No
    Scer\SEC4
    1 of 15
    No
    No
    Scer\TEM1
    1 of 15
    No
    Yes
    Schizosaccharomyces pombe (Fission yeast) (2)
    Spom\ypt1
    1 of 12
    Yes
    No
    Spom\ypt2
    1 of 12
    Yes
    No
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190FJO )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Dmel\Rab35
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00006497
    Drosophila simulans
    Dsim\GD15551
    Drosophila sechellia
    Dsec\GM22691
    Drosophila erecta
    Dere\GG18010
    Drosophila yakuba
    Dyak\GE17342
    Drosophila ananassae
    Dana\GF20451
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA21885
    Drosophila persimilis
    Dper\GL12921
    Drosophila willistoni
    Dwil\GK16335
    Drosophila virilis
    Dvir\GJ19473
    Drosophila mojavensis
    Dmoj\GI14806
    Drosophila grimshawi
    Dgri\GH24326
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AZ1 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17011726
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY003585
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_08789
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes012030
    Aedes aegypti
    Yellow fever mosquito
    Aaeg\AAEL013620
    Anopheles darlingi
    American malaria mosquito
    Adar\ADAC005537
    Anopheles gambiae
    Malaria mosquito
    Agam\AGAP003028
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ014887
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CMD )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Bmor\BGIBMGA012904
    Danaus plexippus
    Monarch butterfly
    Dple\DPOGS206928-PA
    Heliconius melpomene
    Postman butterfly
    Hmel\HMEL015460
    Heliconius melpomene
    Postman butterfly
    Hmel\HMEL015461
    Apis florea
    Little honeybee
    Aflo\2923477220
    Apis mellifera
    Western honey bee
    Amel\LOC100577870
    Bombus impatiens
    Common eastern bumble bee
    Bimp\BIMP25083
    Bombus terrestris
    Buff-tailed bumblebee
    Bter\15202932
    Linepithema humile
    Argentine ant
    Lhum\LH19400
    Megachile rotundata
    Alfalfa leafcutting bee
    Mrot\MROTU:002cdc
    Megachile rotundata
    Alfalfa leafcutting bee
    Mrot\MROTU:000d57
    Nasonia vitripennis
    Parasitic wasp
    Nvit\Nasvi2EG012676
    Dendroctonus ponderosae
    Mountain pine beetle
    Dpod\YQE_08358
    Tribolium castaneum
    Red flour beetle
    Tcas\GLEAN_15416
    Pediculus humanus
    Human body louse
    Phum\PHUM240570
    Rhodnius prolixus
    Kissing bug
    Rpro\RPRC008081
    Cimex lectularius
    Bed bug
    Clec\CLEC008758
    Cimex lectularius
    Bed bug
    Clec\CLEC008980
    Acyrthosiphon pisum
    Pea aphid
    Apim\ACYPI003912
    Zootermopsis nevadensis
    Nevada dampwood termite
    Znev\Znev_03933
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0FML )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Smar\SMAR015600
    Strigamia maritima
    European centipede
    Smar\SMAR003012
    Ixodes scapularis
    Black-legged tick
    Isca\ISCW015757
    Ixodes scapularis
    Black-legged tick
    Isca\ISCW003929
    Ixodes scapularis
    Black-legged tick
    Isca\ISCW006071
    Stegodyphus mimosarum
    African social velvet spider
    Smim\KFM70918.1
    Stegodyphus mimosarum
    African social velvet spider
    Smim\KFM74897.1
    Stegodyphus mimosarum
    African social velvet spider
    Smim\KFM77572.1
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur01g00590
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur01g00570
    Tetranychus urticae
    Two-spotted spider mite
    Turt\tetur19g02010
    Daphnia pulex
    Water flea
    Dpux\DAPPUDRAFT_195992
    Daphnia pulex
    Water flea
    Dpux\DAPPUDRAFT_223052
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LA6 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Spur\Sp-Rab1
    Strongylocentrotus purpuratus
    Purple sea urchin
    Spur\Sp-Rab8
    Strongylocentrotus purpuratus
    Purple sea urchin
    Spur\Sp-Rab35
    Ciona intestinalis
    Vase tunicate
    Cint\LOC100185758
    Ciona intestinalis
    Vase tunicate
    Cint\ENSCING00000016136
    Ciona intestinalis
    Vase tunicate
    Cint\ENSCING00000022390
    Ciona intestinalis
    Vase tunicate
    Cint\LOC100185850
    Gallus gallus
    Domestic chicken
    Ggal\RAB8B
    Gallus gallus
    Domestic chicken
    Ggal\RAB10
    Gallus gallus
    Domestic chicken
    Ggal\ENSGALG00000028851
    Gallus gallus
    Domestic chicken
    Ggal\RAB35
    Gallus gallus
    Domestic chicken
    Ggal\RAB1A
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 1 )
      Allele
      Disease
      Interaction
      References
      Rab35S22N.UAS.YFP
      ameliorates  epilepsy
      modeled by parabss1
      (Kroll et al., 2015)
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      RAB35; RAB35, member RAS oncogene family
      12 of 15
      604199
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Osbp
        suppressible
        Rab35
        (Ma et al., 2012)
        para
        suppressible
        Rab35
        (Kroll et al., 2015)
        sky
        suppressible
        Rab35
        (Uytterhoeven et al., 2011)
        wkd
        suppressible
        Rab35
        (Schottenfeld-Roames and Ghabrial, 2012)
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Gene Group - Pathway Membership (FlyBase)
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-64
        Cytogenetic map
        19B3-19B3
        Sequence location
        X:20,155,766..20,159,872 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        19B3-19B3
        Limits computationally determined from genome sequence between P{EP}amnEP1639&P{EP}amnEP367 and P{EP}CG1702EP1525
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location
        1-64
        (FlyBase, 2016)
        1-63.8
        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (15)
        Genomic Clones (9)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (94)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        polyclonal
        (Shim et al., 2010)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments
        A large increase in the proportion of binucleated cells is seen following transfection of S2 cells with dsRNA made from templates generated with primers directed against this gene.
        (Kouranti et al., 2006)
        S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
        (Stroschein-Stevenson et al., 2006)
        dsRNA made from templates generated with primers directed against Rab35 that is transfected into S2 treated with Listeria monocytogenes reveals Rab35 to be involved in Listeria monocytogenes entry, intracellular growth, and vacuolar escape.
        (Cheng et al., 2005)
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: binucleate cells.
        (Kiger et al., 2003)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 49 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        InterPro - A database of protein families, domains and functional sites
        Linkouts
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMine - An integrated database for Drosophila genomics
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Genes - Molecular building blocks of life in the genomic space.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        modMine - A data warehouse for the modENCODE project
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (8)
        Datasets (1)
        Study focus (1)
        Experimental Role
        Project
        Project Type
        Title
        • bait_protein
        Rab_Effectors_interaction_map
        Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.
        References (85)