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Gene Dmel\RhoGAP19D

General Information
Symbol	Dmel\RhoGAP19D	Species	D. melanogaster
Name	RhoGAP19D	Annotation symbol	CG1412
Feature type	protein_coding_gene	FlyBase ID	FBgn0031118
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	19D1-19D2	Sequence location	X:20,342,656..20,362,133 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol RhoGAP19D (CG1412, FBgn0031118). It has the cytological map location 19D1-19D2. Its sequence location is X:20342656..20362133. Its molecular function is described as protein binding. It is involved in the biological process signal transduction. 9 alleles are reported. The phenotype of these alleles is annotated with embryonic heart. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 19D1-19D2   Limits computationally determined from genome sequence between P{EP}CG1702EP1525 and P{EP}EP1465&P{EP}CG1486EP1192
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\RhoGAP19D for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RhoGAP19D-RA FBtr0077320  NM_134552   7187   2181
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein RhoGAP19D-PA   FBpp0077012   229.8   2181   9.31     NP_608396   AAF50897
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0031118
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RhoGAP (IPR000198) PDZ/DHR/GLGF (IPR001478) Rho GTPase activation protein (IPR008936)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141453   AA695267   AA940599   AA949468   AA951324   AE003571 AAF50897   AI062528   AI114335   AI134110   AI456106   AI520622   AW942971   AY058603 AAL13832   AZ933390   AZ933391   BG632599   BG638283   BG638651   BI162127   BI365602   BI371065   BI625042   BI630744   BI640045   BI641074   BT014632   CK01769   CO155170   CO155708   CO277237   CO285356   CO287750   CO297079   CO304011   CO306328   CO307040   CO312206   CO343079   CZ482161   CZ484675   CZ484874   CZ484875   EC062360   EC076185   EC077489   EC081445   EC243638   EC247752   EC248113   EC260553   EC261986   EC265706   EL872916   EL872917   GH11147   GH11471   LD21832   LD31732
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q95TR0 Q9VRA6
Maps to
Does NOT map to
Identified with	LD29482 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 5259
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI114335 AA940599 AI134110 AA949468
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031118 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0031118 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0031118 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for RhoGAP19D FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for RhoGAP19D Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele embryonic heart RhoGAP19DdsRNA.cKa RhoGAP19DdsRNA.si.cKa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of RhoGAP19D Class Mutagen Stocks Known lesion RhoGAP19DBG01005 P-element activity 1 -- RhoGAP19DdsRNA.H.Scer\UAS 0 -- RhoGAP19De04399 piggyBac transposase 1 -- RhoGAP19Df02168 piggyBac transposase 1 -- RhoGAP19Df02368 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RhoGAP19D Class Mutagen Stocks Known lesion RhoGAP19DGD5226 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes RhoGAP19DdsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 2 Yes RhoGAP19DdsRNA.cKa in vitro construct | RNAi 0 Yes RhoGAP19DdsRNA.si.cKa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)HM44
Useful duplication	Dp(1;1)BSTAG
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5226} NA P{UAS-RhoGAP19D-dsRNA} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}RhoGAP19De04399 NA PBac{WH}RhoGAP19Df02168 NA PBac{WH}RhoGAP19Df02368 NA P{GT1}RhoGAP19DBG01005 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 3 )
Molecular Function
CV term Evidence References
protein binding inferred from electronic annotation with InterPro:IPR001478 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
signal transduction inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RhoGAP19D allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 59337 Drosophila PIMRider - The Drosophila Protein Interaction map • 521152
Orthologs
Genome-wide drosophilid orthologs	Dpse\GA25711 Dana\GF15986 Dere\GG17580 Dgri\GH17777 Dmoj\GI15123 Dper\GL15932 Dsec\GM22667 Dsim\GD15533 Dvir\GJ18980 Dwil\GK20057 Dyak\GE15343
Curated drosophilid orthologs	Dpse\GA12771
Linkouts	InParanoid orthologs - Eukaryotic orthologs • MGI:1918685 Mus musculus (Informatics) WBGene00015418 Caenorhabditis elegans (Wormbase) AT2G46710.1 Arabidopsis thaliana (Arabidopsis) Q7XIX9 Oryza sativa (Gramene) ENSXETP00000032292 Xenopus tropicalis (Ensembl) ENSPTRP00000021399 Pan troglodytes (Ensembl) ENSMMUP00000008593 Macaca mulatta (Ensembl) ENSP00000295095 Homo sapiens (Ensembl) ENSGALP00000020276 Gallus gallus (Ensembl) ENSCINP00000017796 Ciona intestinalis (Ensembl) ENSCAFP00000008184 Canis familiaris (Ensembl) ENSANGP00000028844 Anopheles gambiae (Ensembl)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
VDRC	v43955 w1118 P{GD5226}v43955
Harvard	- PBac{RB}RhoGAP19De04399 - PBac{WH}RhoGAP19Df02168 - PBac{WH}RhoGAP19Df02368
Bloomington	12526 w1118 P{GT1}RhoGAP19DBG01005 6435 y1 w*; P{UAS-RhoGAP19D-dsRNA}2.1 6436 y1 w*; P{UAS-RhoGAP19D-dsRNA}3.1
Genomic Clones ( 1 )
	BACR25I22
cDNA Clones ( 28 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD12316 LD29482
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE01533 GH01754 RH64852 RE50963 GM02509 SD22809 SD24189 RH60035 RE57877 LD35954
Other clones	EK066656 EK123445 EK243415 GH11471 EN06612 EK168826 LD31732 EK228059 EK250807 EP16054 LD21832 EK253340 GH11147 EN05512 EK215852 CK01769
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0031118 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0031118
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_134552 RefSeq (Proteins) • NP_608396 DDBJ / EMBL / GenBank • AA949468 AE003571 AAF50897 AW942971 AY058603 AAL13832 AZ933390 AZ933391 CZ482161 CZ484675 CZ484874 CZ484875 UniProtKB/TrEMBL • Q95TR0 Q9VRA6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RhoGAP (IPR000198) PDZ/DHR/GLGF (IPR001478) Rho GTPase activation protein (IPR008936)
Linkouts
	BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for RhoGAP19D BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 59337 DEDB - Drosophila exon database: splicing graphs • 5259 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0031118 Drosophila PIMRider - The Drosophila Protein Interaction map • 521152 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031118 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0031118 FlyExpress - Embryonic expression images (BDGP data) • FBgn0031118 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031118 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0031118 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0031118 InParanoid orthologs - Eukaryotic orthologs • MGI:1918685 Mus musculus (Informatics) WBGene00015418 Caenorhabditis elegans (Wormbase) AT2G46710.1 Arabidopsis thaliana (Arabidopsis) Q7XIX9 Oryza sativa (Gramene) ENSXETP00000032292 Xenopus tropicalis (Ensembl) ENSPTRP00000021399 Pan troglodytes (Ensembl) ENSMMUP00000008593 Macaca mulatta (Ensembl) ENSP00000295095 Homo sapiens (Ensembl) ENSGALP00000020276 Gallus gallus (Ensembl) ENSCINP00000017796 Ciona intestinalis (Ensembl) ENSCAFP00000008184 Canis familiaris (Ensembl) ENSANGP00000028844 Anopheles gambiae (Ensembl) PANTHER - Protein classification by function, families, and pathways • FBgn0031118 University of Chicago Dev. Expression • FBgn0031118
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	1412 (Hu et al., 2005) CG1412 (Kim et al., 2004, Bernards, 2003, Markstein et al., 2002, Billuart et al., 2001, ) RhoGAP19D
Name Synonym	RhoGAP19D
Secondary FlyBase IDs
References ( 13 )
Research paper	Hu et al., 2005, Proc. Natl. Acad. Sci. USA 102(12): 4613--4618 Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. [FBrf0191476] Kim et al., 2004, Proc. Natl. Acad. Sci. USA 101(1): 159--164 A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryos. [FBrf0168010] Markstein et al., 2002, Proc. Natl. Acad. Sci. USA 99(2): 763--768 Genome-wide analysis of clustered Dorsal binding sites identifies putative target genes in the Drosophila embryo. [FBrf0145207] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610]
Supplementary material	Kiger et al., 2003, J. Biol. 2(4): Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
Review	Bernards, 2003, Biochim. biophys. Acta 1603(2): 47--82 GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. [FBrf0158741]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]