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Gene Dmel\Atg4

General Information
Symbol	Dmel\Atg4	Species	D. melanogaster
Name	Autophagy-specific gene 4	Annotation symbol	CG4428
Feature type	protein_coding_gene	FlyBase ID	FBgn0031298
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	Atg4a
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	21E4-21E4	Sequence location	2L:1,080,911..1,083,378 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Autophagy-specific gene 4 is referred to in FlyBase by the symbol Dmel\Atg4 (CG4428, FBgn0031298). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: cysteine-type endopeptidase activity. There is experimental evidence that it is involved in the biological process: regulation of autophagy. 9 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Peptidase C54. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1641676_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Atg4. Gene sequence location is 2L:1080911..1083378.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21E4-21E4   Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Atg4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Atg4-RA FBtr0077953  NM_134719   2076   411 Atg4-RB FBtr0332569  NM_001272923   2073   410
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Atg4-PA   FBpp0077618   45.6   411   4.53     NP_608563   AAF51423 Atg4-PB   FBpp0304824   45.5   410   4.56     NP_001259852   AGB92389
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C54 (IPR005078)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202569   AA440398   AA539333   AA539652   AA696840   AA803944   AA816645   AA941278   AA942211   AA942595   AE014134 AAF51423   AE014134 AGB92389   AI134501   AI455212   AI455921   AI456358   AI513962   AI515243   AI520070   AI520197   AW942836   AY058573 AAL13802   BI165650   BI167543   BI213030   BI213613   BI231752   BI238352   BI356545   BI366301   BI369548   BI482268   BI568411   BI568663   BI580429   BI592568   BI606267   BI607254   BI614123   BI615963   BI622550   BI627348   BI631509   BP554460   BP554461   CK659032   CO153211   CO156602   CO262472   EC066180   EC066546   EC081874   EC088430   EC219323   EC227652   EC228793   EV596578
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VPW2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca  (768.8) Adult InseminatedFemale Spermatheca  (611.6) Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca  768.8 Adult InseminatedFemale Spermatheca  611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    163.85 Larval Midgut    301.3 Larval Hindgut    231.9 Larval Malpighian Tubules    414.5 Larval Fat Body    451.1 Larval Salivary Gland    279.6 Larval Trachea    194.3 Larval Carcass    392.675 Adult Head    210.1 Adult Eye    188.825 Adult Brain    124.5 Adult Thoracic-Abdominal Ganglion    151.3 Adult Crop    196.1 Adult Midgut    236.7 Adult Hindgut    170.3 Adult Malpighian Tubules    281.9 Adult Fat Body    464.3 Adult Salivary Gland    159.7 Adult Heart    275.4 Adult VirginFemale Spermatheca    768.8 Adult InseminatedFemale Spermatheca    611.6 Adult Ovary    215.4 Adult Testis    43.3 Adult Male Accessory Gland    173.8 Adult Carcass    300.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    210.1   NA  Eye    188.825   NA  Brain    124.5   163.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    151.3   NA  Crop    196.1   301.3  Midgut    236.7   231.9  Hindgut    170.3   414.5  Malpighian Tubules    281.9   451.1  Fat Body    464.3   279.6  Salivary Gland    159.7   NA  Heart    275.4   194.3  Trachea    NA   NA  VirginFemale Spermatheca    768.8   NA  InseminatedFemale Spermatheca    611.6   NA  Ovary    215.4   NA  Testis    43.3   NA  Male Accessory Gland    173.8   392.675  Carcass    300.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female  (28) head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (30) digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering  (36) carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female  28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering  (36) carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    18 central nervous system, pupae P8    9 head, virgin 1-day female    12 head, virgin 4-day female    16 head, virgin 20-day female    28 head, mated 1-day female    15 head, mated 4-day female    14 head, mated 20-day female    21 head, mated 1-day male    20 head, mated 4-day male    18 head, mated 20-day male    21 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    30 digestive system, 1-day adult    14 digestive system, 4-day adult    11 digestive system, 20-day adult    13 fat body, larvae L3 wandering    16 fat body, white prepupae    19 fat body, pupae P8    7 carcass, larvae L3 wandering    36 carcass, 1-day adult    15 carcass, 4-day adult    13 carcass, 20-day adult    19 ovary, virgin 4-day female    20 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (45) embryo 02-04hr  (59) embryo 04-06hr  (45) embryo 06-08hr  (47) embryo 08-10hr  (48) embryo 10-12hr  (52) embryo 12-14hr  (39) embryo 14-16hr  (37) embryo 16-18hr  (33) embryo 18-20hr  (36) embryo 20-22hr  (34) embryo 22-24hr  (36) larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2  (31) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (65) white prepupae new  (75) white prepupae 12hr  (36) white prepupae 24hr  (32) pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day  (31) adult male 05day  (28) adult male 30day    20 adult female 01day  (27) adult female 05day  (30) adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (45) embryo 02-04hr  (59) embryo 04-06hr  (45) embryo 06-08hr  (47) embryo 08-10hr  (48) embryo 10-12hr  (52) embryo 12-14hr  (39) embryo 14-16hr  (37) embryo 16-18hr  (33) embryo 18-20hr  (36) embryo 20-22hr  (34) embryo 22-24hr  (36) larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2  31 larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (65) white prepupae new  (75) white prepupae 12hr  (36) white prepupae 24hr  32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day  31 adult male 05day  28 adult male 30day    20 adult female 01day  27 adult female 05day  30 adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    45 embryo 02-04hr    59 embryo 04-06hr    45 embryo 06-08hr    47 embryo 08-10hr    48 embryo 10-12hr    52 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    33 embryo 18-20hr    36 embryo 20-22hr    34 embryo 22-24hr    36 larva L1    15 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    65 white prepupae new    75 white prepupae 12hr    36 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    17 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    28 adult male 30day    20 adult female 01day    27 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4  26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167  (34) embryonic S1  (35) embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3  (37) eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5  (44) mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2  26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4  26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167  (34) embryonic S1  (35) embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3  (37) eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5  (44) mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2  26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    26 embryonic 1182-4H    20 embryonic GM2    18 embryonic Kc167    34 embryonic S1    35 embryonic S3    19 leg disc CME L1    17 wing disc CME-W2    20 wing disc ML-DmD8    21 wing disc ML-DmD9    24 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    21 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    37 eye-antennal disc ML-DmD11    25 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    26 ovary fGS/OSS    17 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (28) Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    18 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    18 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    22 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD26292 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031298 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4428-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Atg4GD11315 Other Phenotypes Allele starvation stress response defective | late third instar larval stage | somatic clone, with Scer\GAL4Act5C.PP Atg4C98A.Scer\UAS Phenotype manifest in Allele autophagic vacuole | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PP Atg4C98A.Scer\UAS embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PP Atg4C98A.Scer\UAS lysosome | somatic clone | third instar larval stage, with Scer\GAL4Act5C.PP Atg4C98A.Scer\UAS
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Atg4 Class Mutagen Stocks Known lesion Atg4MB03551 1 -- Atg4DG01403 0 -- Atg4G18114 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Atg4 Class Mutagen Stocks Known lesion Atg4GD11315 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg4HMS01482 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg4JF03003 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg4KK108570 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Atg4C98A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Atg4NIG.4428R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6004 (Ryder, 2004.4.26) Df(2L)Exel9037 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11315} NA P{KK108570} NA P{NIG.4428R} NA P{TRiP.HMS01482} NA P{TRiP.JF03003} NA P{UAS-Atg4.C98A} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{wHy}Atg4DG01403 NA insertion of enhancer trap binary system Mi{ET1}Atg4MB03551 No insertion of mobile activating element P{EP}Atg4G18114 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
regulation of autophagy inferred from mutant phenotype (Pircs et al., 2012)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
cysteine-type endopeptidase activity inferred from sequence or structural similarity with Saccharomyces ATG4 (Thumm and Kadowaki, 2001)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Atg4 - Arpc3A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - CG10420 experimental knowledge based (Guruharsha et al., 2011) Atg4 - CG11504 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - CG2493 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - CG32698 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - CG3344 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg4 - CG4407 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - CG6712 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - CG8654 experimental knowledge based (Guruharsha et al., 2011) Atg4 - CG9967 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Cyp6a13 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - Cyp6d4 experimental knowledge based (Guruharsha et al., 2011) Atg4 - Cyp9f2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - Dlc90F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Fer1HCH experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg4 - Gβ13F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Hmgs experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg4 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Hsp60 experimental knowledge based (Guruharsha et al., 2011) Atg4 - ImpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Lst8 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - MRG15 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg4 - ND75 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - Oscillin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - PSR anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - SPE anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - Sec13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - Tap42 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - exu experimental knowledge based (Guruharsha et al., 2011) Atg4 - igl experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Atg4 - isoQC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - l(1)G0469 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Atg4 - mts anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Atg4 - nwk anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Atg4 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59536 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031298 DroID - A comprehensive database of gene and protein interactions. • FBgn0031298 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33283
Orthologs
OrthoDB Orthologs (46) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Z0BRM Dipteran inclusive ortholog search EOG6N30XK Insect inclusive ortholog search EOG6CJTD3 Arthropod inclusive ortholog search EOG602V84 Metazoa inclusive ortholog search EOG6VB6JT
Orthologs in Drosophila Species (EOG6Z0BRM)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Atg4     Drosophila simulans   Dsim\GD23066 Y   Drosophila sechellia   Dsec\GM16788 Y   Drosophila erecta   Dere\GG24763 Y   Drosophila yakuba   Dyak\GE17286 Y   Drosophila ananassae   Dana\GF15159 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18177 Y   Drosophila persimilis   Dper\GL15485 Y   Drosophila willistoni   Dwil\GK24338 Y   Drosophila virilis   Dvir\GJ16311 Y   Drosophila mojavensis   Dmoj\GI17971 Y   Drosophila grimshawi   Dgri\GH13687 Y
Orthologs in non-Drosophila Dipterans (EOG6N30XK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007228   Anopheles gambiae Malaria mosquito  Agam\AGAP008497   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ802174
Orthologs in non-Dipteran Insects (EOG6CJTD3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Atg4   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011159   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20650   Atta cephalotes Leafcutter ant  Acep\ACEP17187   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15958   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15957   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21112   Linepithema humile Argentine ant  Lhum\LH13247   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18362   Solenopsis invicta Red fire ant  Sinv\SINV15823   Acyrthosiphon pisum Pea aphid  Apim\ACYPI065812   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001437   Bombyx mori Silkmoth  Bmor\BGIBMGA006175   Pediculus humanus Human body louse  Phum\PHUM151060   Tribolium castaneum Red flour beetle  Tcas\TC013405
Orthologs in non-Insect Arthropods (EOG602V84)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_194862   Ixodes scapularis Deer tick  Isca\ISCW004712
Orthologs in non-Arthropod Metazoa (EOG6VB6JT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\atg-4.1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006375gn   Danio rerio Zebrafish  Drer\atg4b   Danio rerio Zebrafish  Drer\atg4a   Xenopus tropicalis Western clawed frog  Xtro\atg4b   Xenopus tropicalis Western clawed frog  Xtro\atg4a   Gallus gallus Domestic chicken  Ggal\ATG4A   Gallus gallus Domestic chicken  Ggal\ATG4B   Mus musculus House mouse  Mmus\Atg4a   Mus musculus House mouse  Mmus\Atg4b   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023914   Rattus norvegicus Norway rat  Rnor\Atg4b   Homo sapiens Human  Hsap\ENSG00000168397   Homo sapiens Human  Hsap\ATG4A
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000168397     Hsap\ATG4A
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23066 Drosophila sechellia Dsec\GM16788 Drosophila erecta Dere\GG24763 Drosophila yakuba Dyak\GE17286 Drosophila ananassae Dana\GF15159 Drosophila pseudoobscura pseudoobscura Dpse\GA18177 Drosophila persimilis Dper\GL15485 Drosophila willistoni Dwil\GK24338 Drosophila virilis Dvir\GJ16311 Drosophila mojavensis Dmoj\GI17971 Drosophila grimshawi Dgri\GH13687
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	23542 w1118; Mi{ET1}Atg4MB03551 28367 y1 v1; P{TRiP.JF03003}attP2 35740 y1 sc* v1; P{TRiP.HMS01482}attP2
VDRC	v107317 P{KK108570}VIE-260B v34843 w1118 P{GD11315}v34843
Genomic Clones ( 2 )
	BACR23E23 BACR35O22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 46 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD26292
Other clones	DPiM_LD26292
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH11978 GH26990 GM08514 GM12329 LD02710 LD03037 LD14985 LD17411 LD17932 LD21786 LD25335 LD26910 LD35654 LD36335 LD40015 LD40277 LD46982 LP16460 RE05582 RE07948 RE18334 RE19049 RE23375 RE34760 RE42722 RE51811 RE55761 RE64937 RE74622 RH10290 RH39852 RH40319 RH43426 RH45882 RH54443 RH61093 RH68634 RH72516 RH74276
Other clones	Dmel_neb_005543_0876_1869 EK085430 EN01509 EN08403 FGM230A04
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0031298 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33283
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Atg4 CG4428 (Scott et al., 2004)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_134719 NM_001272923 RefSeq (Proteins) • NP_608563 NP_001259852 DDBJ / EMBL / GenBank • AA942211 AAF51423 AAL13802 AGB92389 AW942836 AY058573 Entrez Gene - A searchable database of RefSeq genes. • 33283 UniProtKB/TrEMBL • Q9VPW2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C54 (IPR005078) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD26292
Linkouts
	BioGRID - A database of protein and genetic interactions • 59536 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031298 DroID - A comprehensive database of gene and protein interactions. • FBgn0031298 DRSC - Results from RNAi screens. • FBgn0031298 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031298 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4428-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031298 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33283 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33283 modMine - Data generated by the modENCODE project. • FBgn0031298
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Atg4 (Kadandale et al., 2010, Jiang et al., 2009, Martin et al., 2007, Kim et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Pircs et al., 2012) atg4 (Shelly et al., 2009, Kim et al., 2010) Atg4a (Érdi et al., 2012, Pircs et al., 2012) CG4428 (Lee et al., 2003, Thumm and Kadowaki, 2001, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2011.7.19, Érdi et al., 2012, Pircs et al., 2012)
Name Synonym	Autophagy-specific gene 4 (Scott et al., 2004)
Secondary FlyBase IDs
References ( 28 )
Generate a list of	All references relating to this gene ( 28 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 9 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 )
Recent research papers ( 2 )
	Pircs et al., 2012, PLoS ONE 7(8): e44214 Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364] Érdi et al., 2012, Autophagy 8(7): 1124--1135 Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. [FBrf0219844] Show all research papers ...