FB2025_01 , released February 20, 2025
Gene: Dmel\Tango14
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General Information
Symbol
Dmel\Tango14
Species
D. melanogaster
Name
Transport and Golgi organization 14
Annotation Symbol
CG4775
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031312
Gene Model Status
Current
Stock Availability
8 publicly available
Enzyme Name (EC)
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] (2.5.1.87)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

l(2)k00619

Key Links
Genomic Location
Cytogenetic map
21F1-21F1
Sequence location
Recombination map
2-2
RefSeq locus
NT_033779 REGION:1158661..1160220
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
contributes_to ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity
inferred from biological aspect of ancestor with PANTHER:PTN000488276
(GO Reference Genome Project, 2011-)
enables transferase activity, transferring alkyl or aryl (other than methyl) groups
inferred from electronic annotation with InterPro:IPR036424
(InterPro Project Members, 2004-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in protein secretion
inferred from mutant phenotype
(Bard et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in dolichol biosynthetic process
inferred from electronic annotation with InterPro:IPR038887
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000488276
(GO Reference Genome Project, 2011-)
involved_in protein glycosylation
inferred from biological aspect of ancestor with PANTHER:PTN000488276
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in endoplasmic reticulum
inferred from direct assay
(Bard et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of dehydrodolichyl diphosphate synthase complex
inferred from electronic annotation with InterPro:IPR038887
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000488276
(GO Reference Genome Project, 2011-)
is_active_in endoplasmic reticulum membrane
inferred from biological aspect of ancestor with PANTHER:PTN000488276
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity
n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate (2.5.1.87)
RHEA 53008:
Summaries
Gene Group (FlyBase)
ISOPRENYL PYROPHOSPHATE SYNTHASES -
Isoprenyl pyrophosphate synthases (IPPSs) are responsible for the synthesis of linear isoprenyl pyrophosphates. They can be classified as cis- or trans-isoprenyl pyrophosphate synthases according to the stereochemical outcome of their products. All-trans C[[10]] geranyl pyrophosphate (GPP) and C[[15]] farnesyl pyrophosphate (FPP) are synthesized by trans-type geranyl pyrophosphate synthase and farnesyl pyrophosphate synthase, respectively. Starting from FPP, a variety of different chain-length all-trans-isoprenyl pyrophosphates and trans,cis-isoprenyl pyrophosphates are generated by the corresponding trans-IPPSs or cis-IPPSs. While short-chain (C15) and medium-chain (C50-55) cis-prenyltransferase complexes are homodimeric, long-chain (C70-120) cis-prenyltransferases are formed by a heteromeric subunit assembly of unknown stoichiometry. (Adapted from PMID:12135472, PMID:26216239, PMID:33077723.)
DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX -
The dehydrodolichyl diphosphate synthase complex catalyzes cis-prenyl chain elongation to produce the poly-prenyl backbone of dolichol. It is composed of two subunits - DHDDS and NUS1 (aka NgBR or Tango14). Although DHDDS is considered the catalytically active subunit, both subunits are necessary for efficient dolichol synthesis. (Adapted from FBrf0249573.)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Tango14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VPX8)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.44

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Tango14-RA
FBtr0077961
NM_134733
1272
278
Tango14-RB
FBtr0331287
NM_001272928
1198
278
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Tango14-PA
FBpp0077626
31.5
278
8.40
Q9VPX8
NP_608577
AAF51407
Tango14-PB
FBpp0303723
31.5
278
8.40
Q9VPX8
NP_001259857
AGB92394
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

278 aa isoforms: Tango14-PA, Tango14-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tango14 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.17

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in endoplasmic reticulum
inferred from direct assay
(Bard et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tango14 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Tango14
Transgenic constructs containing regulatory region of Tango14
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)BSC481
(Christensen et al., 2008.4.15)
Df(2L)Exel7006
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4pnr-MD237
lethal | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal locomotor behavior | adult stage, with Scer\GAL4elav.PU
abnormal neurophysiology, with Scer\GAL4Tub.PU
abnormal neurophysiology | dominant
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
bang sensitive | adult stage, with Scer\GAL4elav.PU
bang sensitive | adult stage, with Scer\GAL4Tub.PU
bang sensitive | adult stage | dominant
decreased body size | dominant | larval stage
decreased body size | larval stage, with Scer\GAL4Tub.PU
decreased body size | P-stage, with Scer\GAL4Tub.PU
decreased body size | P-stage | dominant
decreased cell number | adult stage, with Scer\GAL4ple.PU
flightless, with Scer\GAL4Mef2.PR
increased cell death | adult stage, with Scer\GAL4elav.PU
Phenotype manifest in
Allele
adult brain, with Scer\GAL4elav.PU
dopaminergic PPL1 neuron | adult stage, with Scer\GAL4ple.PU
dopaminergic PPM1 neuron | adult stage, with Scer\GAL4ple.PU
dopaminergic PPM2 neuron | adult stage, with Scer\GAL4ple.PU
projection neuron
projection neuron, with Scer\GAL4Tub.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\NUS1
12 of 14
Yes
Yes
Hsap\DHDDS
2 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Nus1
12 of 14
Yes
Yes
Rnor\Dhdds
2 of 14
No
No
Mus musculus (laboratory mouse) (2)
Mmus\Nus1
11 of 14
Yes
Yes
Mmus\Dhdds
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (1)
Xtro\nus1
9 of 13
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\nus1
13 of 14
Yes
Yes
Drer\dhdds
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\F37B12.3
8 of 14
Yes
Yes
Cele\T01G1.4
2 of 14
No
No
Cele\Y32B12B.8
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP000402
11 of 12
Yes
Yes
Agam\AgaP_AGAP012908
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (11)
Atha\cPT4
2 of 13
Yes
No
Atha\cPT5
2 of 13
Yes
No
Atha\cPT6
2 of 13
Yes
No
Atha\CPT
2 of 13
Yes
No
Atha\LEW1
2 of 13
Yes
Yes
Atha\AT2G23400
1 of 13
No
No
Atha\AT5G58784
1 of 13
No
No
Atha\cPT1
1 of 13
No
No
Atha\cPT8
1 of 13
No
No
Atha\cPT9
1 of 13
No
No
Atha\DXR
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\NUS1
7 of 13
Yes
Yes
Scer\RER2
1 of 13
No
No
Scer\SRT1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\SPBC2A9.06c
8 of 12
Yes
Yes
Spom\SPAC4D7.04c
1 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\uppS
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Tango14. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
Dhdds
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Tango14JF01144
exacerbates  Parkinson's disease 1
modeled by Hsap\SNCAA30P.UAS
(Xue et al., 2022)
Tango14GD481
exacerbates  Parkinson's disease 1
modeled by Hsap\SNCAA30P.UAS
(Xue et al., 2022)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
NUS1; NUS1 dehydrodolichyl diphosphate synthase subunit
12 of 14
610463
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Other Interaction Browsers
Summary of Genetic Interactions
esyN Network Diagram
Show/hide secondary interactors 
(data from AllianceMine provided by esyN)
esyN Network Key:
Suppression
Enhancement
Other Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Bx
enhanceable
Tango14
(Bronstein et al., 2010)
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
External Data
Linkouts
KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-2
Cytogenetic map
21F1-21F1
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
21F1-21F1
Limits computationally determined from genome sequence between P{PZ}l(2)1068510685&P{lacW}Tango14k00619 and P{lacW}RFeSPk11704
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
21F1-21F2
(determined by in situ hybridisation)
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location

2-2

(FlyBase, 2016)

2-1.7

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (8)
Genomic Clones (15)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

        (Bard et al., 2006)

        dsRNA directed against Tango14 inhibits protein secretion in S2 cells, but has no apparent effect on Golgi organisation.

        (Bard et al., 2006)
        Relationship to Other Genes
        Source for database merge of

        Source for merge of: l(2)k00619 BcDNA:SD10843

        (FlyBase, 1996-)
        Additional comments

        Source for merge of l(2)k00619 BcDNA:SD10843 was TrEMBL update ( date:020807 ).

        (FlyBase, 1996-)
        Nomenclature History
        Source for database identify of

        Source for identity of: Tango14 l(2)k00619

        (Bard et al., 2006)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (8)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 43 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (50)