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Gene Dmel\Npc2a

General Information
Symbol	Dmel\Npc2a	Species	D. melanogaster
Name	Niemann-Pick type C-2a	Annotation symbol	CG7291
Feature type	protein_coding_gene	FlyBase ID	FBgn0031381
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	NPC2, npc2a
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	22B8-22B8	Sequence location	2L:1,988,290..1,988,940 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Niemann-Pick type C-2a is referred to in FlyBase by the symbol Dmel\Npc2a (CG7291, FBgn0031381). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sterol binding. There is experimental evidence that it is involved in the biological process: sterol homeostasis; synaptic target recognition; sterol transport; ecdysteroid biosynthetic process. 10 alleles are reported. The phenotype of these alleles is annotated with: Malpighian tubule. It has one annotated transcript and one annotated polypeptide. Protein features are: Immunoglobulin E-set; MD-2-related lipid-recognition (ML) domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, adult hindgut, larval Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, adult ovary, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval hindgut, adult Malpighian tubules, adult fat body, larval trachea, adult spermathecae, adult testis, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625334_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Npc2a. Gene sequence location is 2L:1988290..1988940.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 22B8-22B8   Limits computationally determined from genome sequence between P{lacW}CG17646k11021 and P{EP}EP366
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Npc2a for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.40 Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Npc2a-RA FBtr0077816  NM_134793   651   148
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Npc2a-PA   FBpp0077492   16.0   148   4.95     NP_608637   AAF51319
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • MD-2-related lipid-recognition (ML) domain (IPR003172) Immunoglobulin E-set (IPR014756)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA950384   AA952012   AE014134 AAF51319   AI109245   AI292997   AI293512   AI295274   AI389311   AI402759   AI403158   AY118810 AAM50670   BI171355   BI173276   BI588792   BI606014   BI610011   BI615358   BI615496   BI621812   BI628786   BT001844 AAN71605   CK662502   CO192529   CO272465   CO307404   CO309140   CO329937   CO333088   EC060909   EC203629   EC204900   EC212206   EC215137   EC216686   EC232361   EC240967   EL873968   EL874217   EV596150   EV599577   GH747730   GH748889   GH755922   GH758512   GH759769   GH761142   GH770341   GH772619   GH773440   GH775293   GH776397   GH777233   GH781907   GH782296   GH783315   GH787206   GH790158   GH794388   GH796066   GH796090   GH796965   GH797176   GH797850   GH798604   GH799637   GH799743   GH800431   GH803198   GH804563   GH806013   GH807573   GH808031   GH808333   GH810429   GH812185   GH812850   GH813554   GH816432   GH820992   GH826477   GH827237   GH828497   GH829996   GH832210   GH833190   GH833293   GH835345   GH836218   GH836321   GH838308   GH839425   GH840955   GH841342   GH842361   GH843236   GH843578   GH844345   GH846832   GH849016   GH849489   GH849890   GH851599   GH855623   GH856191   GH859379   GH860688   GH861009   GH861685   GH862619   GH862907   GH865905   GH866140   GH866220   GH866228   GH866337   GH866732   GH867324   GH867728   GH868561   GH869151   GH869442   GH872475   GH873641   GH874177   GH875224   GH875802   GH876508   GH876732   GH879992   GH882183   GH885049   GH885375   GH886249   GH887057   GH887161   GH887277   GH889207   GH889350   GH891382   GH892645   GH892800   GH894377   GH896078   GH897504   GH898395   GH898688   GH899156   GH903026   GH905371   GH905560   GH905989   GH906358   GH908235   GH908852   GH910667   GH911089   GH911453   GH912733   GH913864   GH916669   GH918337   GH918512   GH920781   GH920926   GH923407   GH923553   GH923954   GH927402   GH927999   GH928432   GH929257   GH930940   GH931210   GH931292   GH936884   GH938339   GH940649   GH941022   GH942459
UniProtKB/Swiss-Prot	Q9VQ62
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, adult hindgut, larval Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, adult ovary, adult male accessory gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval hindgut, adult Malpighian tubules, adult fat body, larval trachea, adult spermathecae, adult testis, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body    2356.5 Larval Salivary Gland    1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head    1509.2 Adult Eye    4676.05 Adult Brain    1200.4 Adult Thoracic-Abdominal Ganglion    1747 Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland    2841.4 Adult Heart    1798.575 Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland    1581.6 Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (975.4) Larval Midgut  (946.2) Larval Hindgut    199.3 Larval Malpighian Tubules  (847.5) Larval Fat Body  (2356.5) Larval Salivary Gland  (1034.6) Larval Trachea    270.975 Larval Carcass    161.125 Adult Head  (1509.2) Adult Eye  (4676.05) Adult Brain  (1200.4) Adult Thoracic-Abdominal Ganglion  (1747) Adult Crop    399.2 Adult Midgut  (594.5) Adult Hindgut  (914.4) Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland  (2841.4) Adult Heart  (1798.575) Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary  (733.2) Adult Testis    284 Adult Male Accessory Gland  (1581.6) Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body  (2356.5) Larval Salivary Gland  1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head  (1509.2) Adult Eye  (4676.05) Adult Brain  (1200.4) Adult Thoracic-Abdominal Ganglion  (1747) Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland  (2841.4) Adult Heart  (1798.575) Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland  (1581.6) Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body    2356.5 Larval Salivary Gland    1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head    1509.2 Adult Eye    4676.05 Adult Brain    1200.4 Adult Thoracic-Abdominal Ganglion    1747 Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland    2841.4 Adult Heart    1798.575 Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland    1581.6 Adult Carcass    312.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body    2356.5 Larval Salivary Gland    1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head    1509.2 Adult Eye    4676.05 Adult Brain    1200.4 Adult Thoracic-Abdominal Ganglion    1747 Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland    2841.4 Adult Heart    1798.575 Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland    1581.6 Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (975.4) Larval Midgut  (946.2) Larval Hindgut    199.3 Larval Malpighian Tubules  (847.5) Larval Fat Body  (2356.5) Larval Salivary Gland  (1034.6) Larval Trachea    270.975 Larval Carcass    161.125 Adult Head  (1509.2) Adult Eye  (4676.05) Adult Brain  (1200.4) Adult Thoracic-Abdominal Ganglion  (1747) Adult Crop    399.2 Adult Midgut  594.5 Adult Hindgut  (914.4) Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland  (2841.4) Adult Heart  (1798.575) Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary  733.2 Adult Testis    284 Adult Male Accessory Gland  (1581.6) Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body  (2356.5) Larval Salivary Gland  1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head  1509.2 Adult Eye  (4676.05) Adult Brain  1200.4 Adult Thoracic-Abdominal Ganglion  (1747) Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland  (2841.4) Adult Heart  (1798.575) Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland  1581.6 Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    975.4 Larval Midgut    946.2 Larval Hindgut    199.3 Larval Malpighian Tubules    847.5 Larval Fat Body    2356.5 Larval Salivary Gland    1034.6 Larval Trachea    270.975 Larval Carcass    161.125 Adult Head    1509.2 Adult Eye    4676.05 Adult Brain    1200.4 Adult Thoracic-Abdominal Ganglion    1747 Adult Crop    399.2 Adult Midgut    594.5 Adult Hindgut    914.4 Adult Malpighian Tubules    175.2 Adult Fat Body    185.8 Adult Salivary Gland    2841.4 Adult Heart    1798.575 Adult VirginFemale Spermatheca    328.2 Adult InseminatedFemale Spermatheca    219.9 Adult Ovary    733.2 Adult Testis    284 Adult Male Accessory Gland    1581.6 Adult Carcass    312.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1509.2   NA  Eye    4676.05   NA  Brain    1200.4   975.4  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1747   NA  Crop    399.2   946.2  Midgut    594.5   199.3  Hindgut    914.4   847.5  Malpighian Tubules    175.2   2356.5  Fat Body    185.8   1034.6  Salivary Gland    2841.4   NA  Heart    1798.575   270.975  Trachea    NA   NA  VirginFemale Spermatheca    328.2   NA  InseminatedFemale Spermatheca    219.9   NA  Ovary    733.2   NA  Testis    284   NA  Male Accessory Gland    1581.6   161.125  Carcass    312.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male    101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering    265 digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering    108 fat body, white prepupae    135 fat body, pupae P8    161 carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (29) central nervous system, larvae L3  (58) central nervous system, pupae P8    11 head, virgin 1-day female  (47) head, virgin 4-day female  (44) head, virgin 20-day female  (60) head, mated 1-day female  (49) head, mated 4-day female  (44) head, mated 20-day female  (48) head, mated 1-day male  (77) head, mated 4-day male  (79) head, mated 20-day male  (101) salivary gland, larvae L3 wandering  (35) salivary gland, white prepupae  (90) digestive system, larvae L3 wandering  (265) digestive system, 1-day adult  26 digestive system, 4-day adult    16 digestive system, 20-day adult  (32) fat body, larvae L3 wandering  (108) fat body, white prepupae  (135) fat body, pupae P8  (161) carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female  (60) ovary, mated 4-day female  (56) testis, mated 4-day male  (44) accessory gland, mated 4-day male  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male  101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering  (265) digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering  (108) fat body, white prepupae  (135) fat body, pupae P8  (161) carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male    101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering    265 digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering    108 fat body, white prepupae    135 fat body, pupae P8    161 carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male    101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering    265 digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering    108 fat body, white prepupae    135 fat body, pupae P8    161 carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  29 central nervous system, larvae L3  (58) central nervous system, pupae P8    11 head, virgin 1-day female  (47) head, virgin 4-day female  (44) head, virgin 20-day female  (60) head, mated 1-day female  (49) head, mated 4-day female  (44) head, mated 20-day female  (48) head, mated 1-day male  (77) head, mated 4-day male  (79) head, mated 20-day male  (101) salivary gland, larvae L3 wandering  (35) salivary gland, white prepupae  (90) digestive system, larvae L3 wandering  (265) digestive system, 1-day adult  26 digestive system, 4-day adult    16 digestive system, 20-day adult  32 fat body, larvae L3 wandering  (108) fat body, white prepupae  (135) fat body, pupae P8  (161) carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female  (60) ovary, mated 4-day female  (56) testis, mated 4-day male  (44) accessory gland, mated 4-day male  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male  101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering  (265) digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering  108 fat body, white prepupae  135 fat body, pupae P8  (161) carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    58 central nervous system, pupae P8    11 head, virgin 1-day female    47 head, virgin 4-day female    44 head, virgin 20-day female    60 head, mated 1-day female    49 head, mated 4-day female    44 head, mated 20-day female    48 head, mated 1-day male    77 head, mated 4-day male    79 head, mated 20-day male    101 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    90 digestive system, larvae L3 wandering    265 digestive system, 1-day adult    26 digestive system, 4-day adult    16 digestive system, 20-day adult    32 fat body, larvae L3 wandering    108 fat body, white prepupae    135 fat body, pupae P8    161 carcass, larvae L3 wandering    17 carcass, 1-day adult    21 carcass, 4-day adult    16 carcass, 20-day adult    19 ovary, virgin 4-day female    60 ovary, mated 4-day female    56 testis, mated 4-day male    44 accessory gland, mated 4-day male    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    150 embryo 02-04hr    137 embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr    146 embryo 16-18hr    125 embryo 18-20hr    106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2    125 larva L3 puffstage 3-6    163 larva L3 puffstage 7-9    277 white prepupae new    584 white prepupae 12hr    426 white prepupae 24hr    266 pupae 2d postWPP    171 pupae 3d postWPP    72 pupae 4d postWPP    136 adult male 01day    107 adult male 05day    87 adult male 30day    112 adult female 01day    169 adult female 05day    131 adult female 30day    131 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (150) embryo 02-04hr  (137) embryo 04-06hr  (64) embryo 06-08hr  (28) embryo 08-10hr  (47) embryo 10-12hr  (68) embryo 12-14hr  (60) embryo 14-16hr  (146) embryo 16-18hr  (125) embryo 18-20hr  (106) embryo 20-22hr  (87) embryo 22-24hr  (84) larva L1  (58) larva L2  (34) larva L3 12hr old  (55) larva L3 puffstage 1-2  (125) larva L3 puffstage 3-6  (163) larva L3 puffstage 7-9  (277) white prepupae new  (584) white prepupae 12hr  (426) white prepupae 24hr  (266) pupae 2d postWPP  (171) pupae 3d postWPP  (72) pupae 4d postWPP  (136) adult male 01day  (107) adult male 05day  (87) adult male 30day  (112) adult female 01day  (169) adult female 05day  (131) adult female 30day  (131) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (150) embryo 02-04hr  (137) embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr  (146) embryo 16-18hr  (125) embryo 18-20hr  106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2  (125) larva L3 puffstage 3-6  (163) larva L3 puffstage 7-9  (277) white prepupae new  (584) white prepupae 12hr  (426) white prepupae 24hr  (266) pupae 2d postWPP  (171) pupae 3d postWPP    72 pupae 4d postWPP  (136) adult male 01day  107 adult male 05day    87 adult male 30day  (112) adult female 01day  (169) adult female 05day  (131) adult female 30day  (131) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    150 embryo 02-04hr    137 embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr    146 embryo 16-18hr    125 embryo 18-20hr    106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2    125 larva L3 puffstage 3-6    163 larva L3 puffstage 7-9    277 white prepupae new    584 white prepupae 12hr    426 white prepupae 24hr    266 pupae 2d postWPP    171 pupae 3d postWPP    72 pupae 4d postWPP    136 adult male 01day    107 adult male 05day    87 adult male 30day    112 adult female 01day    169 adult female 05day    131 adult female 30day    131 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    150 embryo 02-04hr    137 embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr    146 embryo 16-18hr    125 embryo 18-20hr    106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2    125 larva L3 puffstage 3-6    163 larva L3 puffstage 7-9    277 white prepupae new    584 white prepupae 12hr    426 white prepupae 24hr    266 pupae 2d postWPP    171 pupae 3d postWPP    72 pupae 4d postWPP    136 adult male 01day    107 adult male 05day    87 adult male 30day    112 adult female 01day    169 adult female 05day    131 adult female 30day    131 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (150) embryo 02-04hr  (137) embryo 04-06hr  (64) embryo 06-08hr  28 embryo 08-10hr  (47) embryo 10-12hr  (68) embryo 12-14hr  (60) embryo 14-16hr  (146) embryo 16-18hr  (125) embryo 18-20hr  (106) embryo 20-22hr  (87) embryo 22-24hr  (84) larva L1  (58) larva L2  (34) larva L3 12hr old  (55) larva L3 puffstage 1-2  (125) larva L3 puffstage 3-6  (163) larva L3 puffstage 7-9  (277) white prepupae new  (584) white prepupae 12hr  (426) white prepupae 24hr  (266) pupae 2d postWPP  (171) pupae 3d postWPP  (72) pupae 4d postWPP  (136) adult male 01day  (107) adult male 05day  (87) adult male 30day  (112) adult female 01day  (169) adult female 05day  (131) adult female 30day  (131) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (150) embryo 02-04hr  137 embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr  (146) embryo 16-18hr  125 embryo 18-20hr  106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2  125 larva L3 puffstage 3-6  (163) larva L3 puffstage 7-9  (277) white prepupae new  (584) white prepupae 12hr  (426) white prepupae 24hr  (266) pupae 2d postWPP  (171) pupae 3d postWPP    72 pupae 4d postWPP  136 adult male 01day  107 adult male 05day    87 adult male 30day  112 adult female 01day  (169) adult female 05day  131 adult female 30day  131 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    150 embryo 02-04hr    137 embryo 04-06hr    64 embryo 06-08hr    28 embryo 08-10hr    47 embryo 10-12hr    68 embryo 12-14hr    60 embryo 14-16hr    146 embryo 16-18hr    125 embryo 18-20hr    106 embryo 20-22hr    87 embryo 22-24hr    84 larva L1    58 larva L2    34 larva L3 12hr old    55 larva L3 puffstage 1-2    125 larva L3 puffstage 3-6    163 larva L3 puffstage 7-9    277 white prepupae new    584 white prepupae 12hr    426 white prepupae 24hr    266 pupae 2d postWPP    171 pupae 3d postWPP    72 pupae 4d postWPP    136 adult male 01day    107 adult male 05day    87 adult male 30day    112 adult female 01day    169 adult female 05day    131 adult female 30day    131 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2    201 embryonic Kc167    281 embryonic S1    121 embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8    112 wing disc ML-DmD9    118 wing disc ML-DmD16-c3    96 wing disc ML-DmD21    598 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    218 eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    252 CNS ML-DmBG1-c1    152 CNS ML-DmBG2-c2    180 tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (74) Schneider line 2 Sg4  (35) embryonic 1182-4H    23 embryonic GM2  (201) embryonic Kc167  (281) embryonic S1  (121) embryonic S3    21 leg disc CME L1  (36) wing disc CME-W2  (33) wing disc ML-DmD8  (112) wing disc ML-DmD9  (118) wing disc ML-DmD16-c3  (96) wing disc ML-DmD21  (598) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (218) eye-antennal disc ML-DmD11  (75) antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1  (252) CNS ML-DmBG1-c1  (152) CNS ML-DmBG2-c2  (180) tumorous blood cells mbn2  (81) ovary fGS/OSS  (41) ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2  (201) embryonic Kc167  (281) embryonic S1  (121) embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8  (112) wing disc ML-DmD9  (118) wing disc ML-DmD16-c3    96 wing disc ML-DmD21  (598) wing disc ML-DmD32    33 haltere disc ML-DmD17-c3  (218) eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1  (252) CNS ML-DmBG1-c1  (152) CNS ML-DmBG2-c2  (180) tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2    201 embryonic Kc167    281 embryonic S1    121 embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8    112 wing disc ML-DmD9    118 wing disc ML-DmD16-c3    96 wing disc ML-DmD21    598 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    218 eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    252 CNS ML-DmBG1-c1    152 CNS ML-DmBG2-c2    180 tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2    201 embryonic Kc167    281 embryonic S1    121 embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8    112 wing disc ML-DmD9    118 wing disc ML-DmD16-c3    96 wing disc ML-DmD21    598 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    218 eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    252 CNS ML-DmBG1-c1    152 CNS ML-DmBG2-c2    180 tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (74) Schneider line 2 Sg4  (35) embryonic 1182-4H    23 embryonic GM2  (201) embryonic Kc167  (281) embryonic S1  (121) embryonic S3    21 leg disc CME L1  (36) wing disc CME-W2  (33) wing disc ML-DmD8  (112) wing disc ML-DmD9  (118) wing disc ML-DmD16-c3  (96) wing disc ML-DmD21  (598) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (218) eye-antennal disc ML-DmD11  (75) antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1  (252) CNS ML-DmBG1-c1  (152) CNS ML-DmBG2-c2  (180) tumorous blood cells mbn2  (81) ovary fGS/OSS  (41) ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2  (201) embryonic Kc167  (281) embryonic S1  121 embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8  112 wing disc ML-DmD9  118 wing disc ML-DmD16-c3    96 wing disc ML-DmD21  (598) wing disc ML-DmD32    33 haltere disc ML-DmD17-c3  (218) eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1  (252) CNS ML-DmBG1-c1  (152) CNS ML-DmBG2-c2  (180) tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    35 embryonic 1182-4H    23 embryonic GM2    201 embryonic Kc167    281 embryonic S1    121 embryonic S3    21 leg disc CME L1    36 wing disc CME-W2    33 wing disc ML-DmD8    112 wing disc ML-DmD9    118 wing disc ML-DmD16-c3    96 wing disc ML-DmD21    598 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    218 eye-antennal disc ML-DmD11    75 antennal disc ML-DmD20-c5    14 mixed discs ML-DmD4-c1    252 CNS ML-DmBG1-c1    152 CNS ML-DmBG2-c2    180 tumorous blood cells mbn2    81 ovary fGS/OSS    41 ovary OSC    9 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (59) cold shock, 4-day adult  (57) heat shock, 4-day adult  (51) Cadmium 50 mM 6 hrs, larvae L3  (47) Cadmium 50 mM 12 hrs, larvae L3  (95) Cadmium 50 mM 48 hrs, 4-day adult  (32) Cadmium 100 mM 48 hrs, 4-day adult  (43) Copper 0.5 mM 12 hrs, larvae L3  (37) Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3  (46) Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  (75) Ethanol 5% 3 hrs, larvae L3  (40) Ethanol 10% 3 hrs, larvae L3  (42) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (55) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (40) Caffeine 25 mg/ml 48 hrs, 4-day adult  (38) Paraquat 5 mM 48 hrs, 4-day adult  (53) Paraquat 10 mM 48 hrs, 4-day adult  (64) Rotenone 2 μg 12 hrs, larvae L3  (51) Rotenone 8 μg 12 hrs, larvae L3  (54) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (59) cold shock, 4-day adult  (57) heat shock, 4-day adult  (51) Cadmium 50 mM 6 hrs, larvae L3  (47) Cadmium 50 mM 12 hrs, larvae L3  (95) Cadmium 50 mM 48 hrs, 4-day adult  32 Cadmium 100 mM 48 hrs, 4-day adult  (43) Copper 0.5 mM 12 hrs, larvae L3  (37) Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3  (46) Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  (75) Ethanol 5% 3 hrs, larvae L3  (40) Ethanol 10% 3 hrs, larvae L3  (42) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (55) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (40) Caffeine 25 mg/ml 48 hrs, 4-day adult  (38) Paraquat 5 mM 48 hrs, 4-day adult  (53) Paraquat 10 mM 48 hrs, 4-day adult  (64) Rotenone 2 μg 12 hrs, larvae L3  (51) Rotenone 8 μg 12 hrs, larvae L3  (54) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    59 cold shock, 4-day adult    57 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    47 Cadmium 50 mM 12 hrs, larvae L3    95 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    24 Zinc 5 mM 12 hrs, larvae L3    46 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    75 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    42 Caffeine 1.5 mg/ml 4 hrs, larvae L3    55 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    40 Caffeine 25 mg/ml 48 hrs, 4-day adult    38 Paraquat 5 mM 48 hrs, 4-day adult    53 Paraquat 10 mM 48 hrs, 4-day adult    64 Rotenone 2 μg 12 hrs, larvae L3    51 Rotenone 8 μg 12 hrs, larvae L3    54 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031381 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7291-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Npc2a239 Npc2a271 Npc2a376 viable, with Scer\GAL4pnr-MD237 Npc2aGD5075 Sterility Allele fertile Npc2a239 Npc2a271 Npc2a376 Other Phenotypes Allele short lived Npc2a239 Npc2a271 Npc2a376 Phenotype manifest in Allele Malpighian tubule Npc2aunspecified
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Npc2a Class Mutagen Stocks Known lesion Npc2a376 P-element activity 1 Yes Npc2ad04217 1 -- Npc2a239 P-element activity 0 Yes Npc2a271 P-element activity 0 Yes Npc2aunspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Npc2a Class Mutagen Stocks Known lesion Npc2aGD5075 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Npc2aHMS01681 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Npc2aKK108573 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Npc2aNIG.7291R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Npc2aScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6006 (Ryder, 2004.4.26) Df(2L)Exel8005 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK108573} NA P{NIG.7291R} NA P{TRiP.HMS01681} NA P{UAS-Npc2a.H} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH1929SH1929 No miscellaneous insertions P{SUPor-P}KG05307 NA insertion of mobile activating element P{XP}d04217 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
ecdysteroid biosynthetic process inferred from genetic interaction with Npc2b (Huang et al., 2007) sterol homeostasis inferred from mutant phenotype (Huang et al., 2007) sterol transport inferred from mutant phenotype (Huang et al., 2007) synaptic target recognition inferred from mutant phenotype (Kurusu et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
sterol binding inferred from sequence or structural similarity with human NPC2 (Huang et al., 2007)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Npc2a - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG10418 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - Elongin-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - FK506-bp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Npc2a - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Npc2a allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59609 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031381 DroID - A comprehensive database of gene and protein interactions. • FBgn0031381 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33374
Orthologs
OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6C5D52 Dipteran inclusive ortholog search EOG6FXRHZ Insect inclusive ortholog search EOG6FQZPB Arthropod inclusive ortholog search EOG6FBG9K Metazoa inclusive ortholog search EOG6N9414
Orthologs in Drosophila Species (EOG6C5D52)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Npc2a     Drosophila simulans   Dsim\GD23124 Y   Drosophila sechellia   Dsec\GM16844 Y   Drosophila erecta   Dere\GG24820 Y   Drosophila yakuba   Dyak\GE17856 Y   Drosophila ananassae   Dana\GF14593 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20242 Y   Drosophila persimilis   Dper\GL19668 Y   Drosophila willistoni   Dwil\GK24197 Y   Drosophila virilis   Dvir\GJ22897 Y   Drosophila mojavensis   Dmoj\GI18242 Y   Drosophila mojavensis   Dmoj\GI17283     Drosophila grimshawi   Dgri\GH25210 Y
Orthologs in non-Drosophila Dipterans (EOG6FXRHZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006854   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004652
Orthologs in non-Dipteran Insects (EOG6FQZPB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC551922   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000273   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14321   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14320   Atta cephalotes Leafcutter ant  Acep\ACEP18880   Atta cephalotes Leafcutter ant  Acep\ACEP18877   Camponotus floridanus Florida carpenter ant  Cflo\CFLO11348   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14080   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23399   Linepithema humile Argentine ant  Lhum\LH13000   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14296   Solenopsis invicta Red fire ant  Sinv\SINV20084   Solenopsis invicta Red fire ant  Sinv\SINV20094   Bombyx mori Silkmoth  Bmor\BGIBMGA008405   Tribolium castaneum Red flour beetle  Tcas\ML1
Orthologs in non-Insect Arthropods (EOG6FBG9K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_209126   Ixodes scapularis Deer tick  Isca\ISCW006079   Ixodes scapularis Deer tick  Isca\ISCW016201   Ixodes scapularis Deer tick  Isca\ISCW005419
Orthologs in non-Arthropod Metazoa (EOG6N9414)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011368gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000596gn Y Danio rerio Zebrafish  Drer\LOC565099 Y Xenopus tropicalis Western clawed frog  Xtro\npc2 Y Gallus gallus Domestic chicken  Ggal\NPC2 Y Mus musculus House mouse  Mmus\Npc2 Y Rattus norvegicus Norway rat  Rnor\Npc2 Y Homo sapiens Human  Hsap\NPC2 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\NPC2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23124 Drosophila sechellia Dsec\GM16844 Drosophila erecta Dere\GG24820 Drosophila yakuba Dyak\GE17856 Drosophila ananassae Dana\GF14593 Drosophila pseudoobscura pseudoobscura Dpse\GA20242 Drosophila persimilis Dper\GL19668 Drosophila willistoni Dwil\GK24197 Drosophila virilis Dvir\GJ22897 Drosophila mojavensis Dmoj\GI18242 Drosophila grimshawi Dgri\GH25210
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	41760 w*; Npc2a376 38237 y1 sc* v1; P{TRiP.HMS01681}attP40
Harvard	- P{XP}d04217
VDRC	v106771 P{KK108573}VIE-260B v30722 w1118; P{GD5075}v30722 v30725 w1118; P{GD5075}v30725
Genomic Clones ( 1 )
	BACR19N22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 170 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH22047 RH53228
Other clones	DPiM_GH22047
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH08376 GH16057 GH20450 GH22541 LD28922 LD29016 LP06675 LP08859 LP22770 RE13108 RE16064 RH14873 RH30221 RH44965 RH45159 RH57533 RH72084
Other clones	149180 Dmel_neb_010456_0625_2376 Dmel_neb_011014_0839_3506 EC33970 EK107251 EK254519 EK260423 EK294210 EK305645 IP14047 IP14247 RP003012940 RP003026066 RP003045976 RP003057337 RP003057601 RP003060348 RP003061512 RP003063266 RP003074735 RP003087112 RP003090014 RP003098353 RP003099380 RP003103277 RP003103551 RP003105676 RP003107918 RP003121482 RP003125936 RP003137143 RP003138994 RP003139238 RP003143893 RP003144698 RP003144784 RP003147778 RP003158224 RP003160139 RP003169242 RP003170459 RP003175202 RP003178860 RP003182951 RP003186163 RP003200214 RP003200405 RP003207213 RP003208153 RP003213263 RP003213363 RP003217776 RP003219161 RP003221411 RP003228670 RP003228785 RP003232428 RP003237651 RP003239040 RP003248337 RP003250790 RP003254715 RP003255420 RP003268875 RP003271374 RP003294264 RP003301100 RP003303396 RP003304398 RP003304923 RP003306473 RP003309970 RP003319502 RP003319914 RP003320181 RP003322463 RP003328411 RP003329591 RP003332228 RP003339253 RP003343452 RP003347354 RP003347467 RP003347499 RP003349596 RP003353150 RP003353873 RP003359889 RP003376007 RP003398050 RP003398601 RP003403432 RP003404286 RP003418337 RP003426051 RP003431018 RP003434041 RP003437663 RP003445508 RP003455783 RP003460534 RP003460769 RP003461777 RP003463116 RP003469939 RP003484614 RP003487709 RP003488148 RP003504096 RP003504202 RP003507308 RP003507779 RP003512493 RP003521386 RP003527993 RP003535240 RP003541301 RP003546392 RP003548350 RP003553870 RP003561088 RP003564669 RP003564847 RP003570058 RP003571406 RP003574914 RP003581839 RP003582694 RP003585940 RP003588266 RP003590892 RP003592462 RP003604928 RP003606508 RP003616363 RP003618221 RP003620345 RP003621747 RP003624173 RP003626983 RP003628855 RP003651560 RP003659472 RP003659569 RP003663109 RP003664379 RP003672634 RP003674146 RP003674442 RP003676101
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0031381 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33374
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Npc2a NPC2 (Huang et al., 2007) Source for identity of: NPC2 CG7291 (Huang and Scott, 2004)
Source for database merge of	Source for merge of: CG7291 BcDNA:GH22047 (FlyBase, 1996-) Source for merge of: CG7291 BcDNA:RH53228 (FlyBase, 1996-) Source for merge of: NPC2 BEST:GH08376 (Rana, 2004.7.8)
Additional comments	Source for merge of CG7291 BcDNA:GH22047 was a shared cDNA (date:030728). (FlyBase, 1996-) Source for merge of CG7291 BcDNA:RH53228 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_134793 RefSeq (Proteins) • NP_608637 DDBJ / EMBL / GenBank • AAF51319 AAM50670 AAN71605 AI109245 AI402759 AY118810 BI621812 BT001844 Entrez Gene - A searchable database of RefSeq genes. • 33374 UniProtKB/Swiss-Prot • Q9VQ62
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • MD-2-related lipid-recognition (ML) domain (IPR003172) Immunoglobulin E-set (IPR014756)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59609 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031381 DroID - A comprehensive database of gene and protein interactions. • FBgn0031381 DRSC - Results from RNAi screens. • FBgn0031381 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031381 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7291-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031381 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33374 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/niemann-pick.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33374 modMine - Data generated by the modENCODE project. • FBgn0031381
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	BcDNA:GH22047   BcDNA:RH53228   BEST:GH08376   CG7291 (Rana, 2004.7.8, Inlow and Restifo, 2004, Kurusu et al., 2008, Huang et al., 2007, Keleman et al., 2009.8.5, Cammarato et al., 2011, Lowery et al., 2010, Ni et al., 2010.12.1) CT22505 (Ceriani et al., 2002) DmML3 (Inohara, 2002) dnpc2a (Huang et al., 2006) NPC2 (Kurusu et al., 2008, Comet et al., 2006, Beckstead et al., 2007, Japanese National Institute of Genetics, 2012.5.21) npc2a (Huang et al., 2007, Shi et al., 2012) Npc2a
Name Synonym	Epididymal secretory protein (Kang et al., 2002) Niemann-Pick Type C-2 (Huang and Scott, 2004, Cammarato et al., 2011) Niemann-Pick type C-2a
Secondary FlyBase IDs
	FBgn0043830 FBgn0063229 FBgn0064798
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 6 ) Review  ( 1 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Shi et al., 2012, Insect Biochem. Mol. Biol. 42(8): 545--556 Drosophila melanogaster NPC2 proteins bind bacterial cell wall components and may function in immune signal pathways. [FBrf0218672] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...