Gene Dmel\CG4259

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General Information

Symbol

Dmel\CG4259

Species

D. melanogaster

Name

Annotation symbol

CG4259

Feature type

protein_coding_gene

FlyBase ID

FBgn0031389

Gene Model Status

Current

Stock availability

6 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

22C3-22C3

Sequence location

2L:2,127,345..2,129,237 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG4259 (FBgn0031389). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: serine-type endopeptidase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: proteolysis. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Peptidase S1; Trypsin-like cysteine/serine peptidase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, at stages throughout the larval period, during early pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult heart, adult fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636689_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG4259. Gene sequence location is 2L:2127345..2129237.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

22C3-22C3

Limits computationally determined from genome sequence between P{lacW}CG31672^k09932 and P{PZ}aop^03953a&P{EP}aop^EP2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG4259 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG4259-RA

FBtr0077854

NM_134801

964

270

CG4259-RB

FBtr0334781

NM_001272975

1831

270

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG4259-PA

FBpp0077526

30.5

270

6.91

NP_608645

AAF51304

CG4259-PB

FBpp0306834

30.5

270

6.91

NP_001259904

AGB92441

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Peptidase S1 (IPR001254)

Trypsin-like cysteine/serine peptidase domain (IPR009003)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VQ75

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult heart, adult fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		(798.3)
Adult Salivary Gland		7
Adult Heart		(564.4)
Adult VirginFemale Spermatheca		(797)
Adult InseminatedFemale Spermatheca		(1022.5)
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		(1022.5)
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2.575
Larval Midgut		27.4
Larval Hindgut		20.7
Larval Malpighian Tubules		41.6
Larval Fat Body		97.3
Larval Salivary Gland		8.5
Larval Trachea		36.95
Larval Carcass		76.175
Adult Head		101.3
Adult Eye		38.075
Adult Brain		15.1
Adult Thoracic-Abdominal Ganglion		10.8
Adult Crop		8.1
Adult Midgut		8.8
Adult Hindgut		20.9
Adult Malpighian Tubules		8.5
Adult Fat Body		798.3
Adult Salivary Gland		7
Adult Heart		564.4
Adult VirginFemale Spermatheca		797
Adult InseminatedFemale Spermatheca		1022.5
Adult Ovary		3.7
Adult Testis		23
Adult Male Accessory Gland		3.9
Adult Carcass		314.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	101.3
NA	Eye	38.075
NA	Brain	15.1
2.575	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	no informative data
NA	Crop	8.1
27.4	Midgut	8.8
20.7	Hindgut	20.9
41.6	Malpighian Tubules	8.5
97.3	Fat Body	798.3
8.5	Salivary Gland	7
NA	Heart	564.4
36.95	Trachea	NA
NA	VirginFemale Spermatheca	797
NA	InseminatedFemale Spermatheca	1022.5
NA	Ovary	3.7
NA	Testis	23
NA	Male Accessory Gland	3.9
76.175	Carcass	314.5

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		4
head, virgin 4-day female		5
head, virgin 20-day female		5
head, mated 1-day female		4
head, mated 4-day female		9
head, mated 20-day female		5
head, mated 1-day male		2
head, mated 4-day male		2
head, mated 20-day male		1
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		5
carcass, larvae L3 wandering		1
carcass, 1-day adult		4
carcass, 4-day adult		5
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, at stages throughout the larval period, during early pupal stages, in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		0
embryo 16-18hr		1
embryo 18-20hr		1
embryo 20-22hr		3
embryo 22-24hr		4
larva L1		5
larva L2		5
larva L3 12hr old		8
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		1
white prepupae 24hr		3
pupae 2d postWPP		4
pupae 3d postWPP		3
pupae 4d postWPP		2
adult male 01day		6
adult male 05day		3
adult male 30day		2
adult female 01day		5
adult female 05day		3
adult female 30day		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		140
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		(55)
embryonic 1182-4H		10
embryonic GM2		(40)
embryonic Kc167		(140)
embryonic S1		(48)
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		(94)
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		(140)
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		140
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		140
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		(55)
embryonic 1182-4H		10
embryonic GM2		(40)
embryonic Kc167		(140)
embryonic S1		(48)
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		(94)
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		140
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		55
embryonic 1182-4H		10
embryonic GM2		40
embryonic Kc167		140
embryonic S1		48
embryonic S3		26
leg disc CME L1		22
wing disc CME-W2		8
wing disc ML-DmD8		1
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		0
wing disc ML-DmD32		3
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1
antennal disc ML-DmD20-c5		14
mixed discs ML-DmD4-c1		3
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		23
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		0
cold shock, 4-day adult		0
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		4
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		2
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		1
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		4
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		4
Caffeine 25 mg/ml 48 hrs, 4-day adult		0
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		4
Rotenone 8 μg 12 hrs, larvae L3		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

SD22430

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0031389

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4259-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

CG4259^GD821

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG4259	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG4259	Mutagen	Stocks	Known lesion
CG4259^GD821	in vitro construct - RNAi in vitro construct - regulatory fusion	5	Yes
CG4259^KK103510	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG4259^NIG.4259R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)Exel6006

(Ryder, 2004.4.26)

Df(2L)Exel7008

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD821}

P{KK103510}

P{NIG.4259R}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}EY03636

P{EPgy2}EY04012

miscellaneous insertions

P{SUPor-P}KG07454

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

Evidence

References

NOT
serine-type endopeptidase activity

non-traceable author statement

(Ross et al., 2003)

serine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001254

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

proteolysis

inferred from electronic annotation with InterPro:IPR001254

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG4259 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0031389

Orthologs

OrthoDB Orthologs (6) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6MKP2K

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6MKP2K)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (5) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

VDRC

v106161

P{KK103510}VIE-260B

v2541

w¹¹¹⁸ P{GD821}v2541

v44873

w¹¹¹⁸; P{GD821}v44873

v44874

w¹¹¹⁸; P{GD821}v44874

v45271

w¹¹¹⁸; P{GD821}v45271

v52657

w¹¹¹⁸; P{GD821}v52657

Genomic Clones ( 1 )

BACR01K07

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 14 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

HL05366

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0031389

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

33385

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

33385

UniProtKB/TrEMBL

•

Q9VQ75

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Peptidase S1 (IPR001254)

Trypsin-like cysteine/serine peptidase domain (IPR009003)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

SD22430

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0031389

DRSC - Results from RNAi screens.

•

FBgn0031389

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0031389

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4259-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0031389

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

33385

modMine - Data generated by the modENCODE project.

•

FBgn0031389

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

CG4259

(Castillo et al., 2011, Kwon et al., 2008, Wertheim et al., 2011, Keleman et al., 2009.8.5, Behm-Ansmant et al., 2006, Wertheim et al., 2005, Johansson et al., 2005, Japanese National Institute of Genetics, 2012.5.21, Kalajdzic et al., 2012)

SPH173

(Ross et al., 2003)

Name Synonym

Secondary FlyBase IDs

References ( 23 )

Generate a list of

All references relating to this gene ( 23 )

List References by type

Recent research papers ( 4 )

Kalajdzic et al., 2012, PLoS ONE 7(6): e40296: Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms. [FBrf0218826]
Castillo et al., 2011, BMC Evol. Biol. 11: 258: Molecular evolution under increasing transposable element burden in Drosophila: A speed limit on the evolutionary arms race. [FBrf0216257]
Chen et al., 2011, Fly 5(4): 345--351: Roles of young serine-endopeptidase genes in survival and reproduction revealed rapid evolution of phenotypic effects at adult stages. [FBrf0217834]
Wertheim et al., 2011, Mol. Ecol. 20(5): 932--949: Functional genomics of the evolution of increased resistance to parasitism in Drosophila. [FBrf0213049]

Show all research papers ...