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Gene Dmel\VGlut

General Information
Symbol	Dmel\VGlut	Species	D. melanogaster
Name	Vesicular glutamate transporter	Annotation symbol	CG9887
Feature type	protein_coding_gene	FlyBase ID	FBgn0031424
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	DVGLUT
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	22E1-22E1	Sequence location	2L:2,400,295..2,410,662 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vesicular glutamate transporter is referred to in FlyBase by the symbol Dmel\VGlut (CG9887, FBgn0031424). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: neurotransmitter transporter activity. There is experimental evidence that it is involved in the biological process: synaptic transmission, glutamatergic. 57 alleles are reported. The phenotypes of these alleles are annotated with: neuromuscular junction; neuropil; embryonic/larval neuromuscular junction; antennal lobe; synaptic vesicle. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Major facilitator superfamily; Major facilitator superfamily domain; Major facilitator superfamily domain, general substrate transporter. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1632620_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of VGlut. Gene sequence location is 2L:2400295..2410662.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Controlled Vocabulary Terms vesicle References Séjourné et al., 2011, Nat. Neurosci. 14(7): 903--910 Li et al., 2010, EMBO J. 29(5): 992--1006 Das et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(36): E646--E654 Shim et al., 2011, Biochem. J. 439(2): 277--286 Sequence features HFA00733 BKN27498
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 22E1-22E1   Limits computationally determined from genome sequence between P{lacW}Rab5k08232 and P{PZ}dpp10638
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\VGlut for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	EST data suggested additional 5' exons so merged these with gene model 66bp.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) VGlut-RA FBtr0077769  NM_134837   5501   632 VGlut-RB FBtr0307078     5660   632
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein VGlut-PA   FBpp0077449   68.9   632   6.50     NP_608681   AAF51256 VGlut-PB   FBpp0297921   68.9   632   6.50
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Major facilitator superfamily (IPR011701) Major facilitator superfamily domain, general substrate transporter (IPR016196) Major facilitator superfamily domain (IPR020846)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF51256   BI564278   BI585626   BI586836   BI607468   BI613993   BI628904   BT003440 AAO39443   CO178986   CO179853   CO187416   CO187896   CO188546   CO264516   CO272598   CO283362   CO292556   CO292631   CO293297   CO299381   CO301575   CO308734   CO309334   CO309584   CO320345   CO321835   CO325603   CO327987   CO331850   CO339534   CO343933   DN154201   EC057670   EC060745   EC061630   EC062786   EC063457   EC069033   EC070667   EC079083   EC084931   EC086334   EC087268   EC090572   EC199716   EC201001   EC201929   EC203770   EC207289   EC212555   EC216025   EC218125   EC224123   EC231154   EC240910   EC247022   EC253174   EC253838   EC254171   EC258944
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q86P76 Q9VQC0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
	Strong expression of VGlut is seen in cells similar to alpha-processes of the amacrine neurons or possibly like beta-processes of T1 neurons. Weak VGlut expression is seen in cell bodies in the chiasma between lamina and medulla in a position corresponding to the amacrine cells. (Kolodziejczyk et al., 2008)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17   embryonic/larval neuromuscular junction   (Banovic et al., 2010) wandering third instar larval stage   glutamatergic neuron   (Daniels et al., 2008) motor neuron   (Daniels et al., 2008) glutamatergic neuron of ventral nerve cord   (Daniels et al., 2008) embryonic/larval subesophageal ganglion   (Daniels et al., 2008) embryonic/larval brain   (Daniels et al., 2008) embryonic/larval optic lobe   (Daniels et al., 2008) mushroom body vertical lobe | surrounding   (Daniels et al., 2008) mushroom body pedunculus | surrounding   (Daniels et al., 2008) mushroom body vertical lobe | faint   (Daniels et al., 2008) mushroom body medial lobe | faint   (Daniels et al., 2008) mushroom body spur | faint   (Daniels et al., 2008) larval DN1 neuron   (Daniels et al., 2008) adult stage   optic lobe   (Daniels et al., 2008) medulla   (Daniels et al., 2008) lobula   (Daniels et al., 2008) lobula plate   (Daniels et al., 2008) adult antennal lobe   (Daniels et al., 2008) adult central complex   (Daniels et al., 2008) fan-shaped body   (Daniels et al., 2008) nodulus   (Daniels et al., 2008) ellipsoid body   (Daniels et al., 2008) ring neuron   (Daniels et al., 2008) protocerebral bridge   (Daniels et al., 2008) adult subesophageal ganglion   (Daniels et al., 2008) glutamatergic neuron   (Daniels et al., 2008) superior medial protocerebrum   (Daniels et al., 2008) ventrolateral protocerebrum   (Daniels et al., 2008) mushroom body spur | faint   (Daniels et al., 2008) mushroom body alpha-lobe | faint   (Daniels et al., 2008) mushroom body gamma-lobe | faint   (Daniels et al., 2008) mushroom body calyx | faint   (Daniels et al., 2008)
Additional Descriptive Data
	Expression of VGlut protein is seen extensively throughout the central nervous system of the third instar larval brain, with approximately 200 in each brain lobe and 800 in the ventral nerve cord. High VGlut protein expression is reported to be found within the following larval brain compartments: dorsal compartments - dorsoanterior, dorsoposterior, and centroposterior-lateral (dorsal); central compartments - centroanterior, centroposterior-lateral, centroposterior- medial, and centroposterior-intermediate; and basal compartments - centroanterior (ventral), basoanterior, basocentral, basocervical, basoposterior-lateral, and basoposterior-medial (BPM). Highest expression is found in the dorsoanterior and centroanterior compartments. Although VGlut protein is generally not expressed in the larval mushroom body, a small number of punctae are seen in the vertical lobes, medial lobes and the spur. Staining within the ventral nerve cord is predominantly on the dorsal and lateral surface and is largely excluded from the ventral aspect of the neuropil. In the adult brain, expression of VGlut but shows extensive expression in central neuropil compartments, but is predominantly absent from the mushroom body only being detectable as punctae in the base of the gamma-lobe, the tip and base of the alpha-lobe, the spur, and faintly in the calyx. Expression within the ellipsoid body is observed within its inner and outer layers, a pattern suggestive of R-cell innervation. Expression of VGlut is observed in three prominent layers of the medulla - two in the outer medulla, and one encompassing the inner medulla. (Daniels et al., 2008)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0031424    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0031424 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0031424 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr  (170) embryo 14-16hr  (548) embryo 16-18hr  (1158) embryo 18-20hr  (1269) embryo 20-22hr  (1147) embryo 22-24hr  (1440) larva L1  (1183) larva L2  (471) larva L3 12hr old  (142) larva L3 puffstage 1-2    63 larva L3 puffstage 3-6  (175) larva L3 puffstage 7-9  (211) white prepupae new  (231) white prepupae 12hr  (449) white prepupae 24hr  (563) pupae 2d postWPP  (666) pupae 3d postWPP  (671) pupae 4d postWPP  (485) adult male 01day  (413) adult male 05day  (396) adult male 30day  (347) adult female 01day  (197) adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr  1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0031424 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr  (170) embryo 14-16hr  (548) embryo 16-18hr  (1158) embryo 18-20hr  (1269) embryo 20-22hr  (1147) embryo 22-24hr  (1440) larva L1  (1183) larva L2  (471) larva L3 12hr old  142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6  (175) larva L3 puffstage 7-9  (211) white prepupae new  (231) white prepupae 12hr  (449) white prepupae 24hr  (563) pupae 2d postWPP  (666) pupae 3d postWPP  (671) pupae 4d postWPP  (485) adult male 01day  (413) adult male 05day  (396) adult male 30day  (347) adult female 01day  (197) adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr  1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    3 embryo 06-08hr    9 embryo 08-10hr    18 embryo 10-12hr    68 embryo 12-14hr    170 embryo 14-16hr    548 embryo 16-18hr    1158 embryo 18-20hr    1269 embryo 20-22hr    1147 embryo 22-24hr    1440 larva L1    1183 larva L2    471 larva L3 12hr old    142 larva L3 puffstage 1-2    63 larva L3 puffstage 3-6    175 larva L3 puffstage 7-9    211 white prepupae new    231 white prepupae 12hr    449 white prepupae 24hr    563 pupae 2d postWPP    666 pupae 3d postWPP    671 pupae 4d postWPP    485 adult male 01day    413 adult male 05day    396 adult male 30day    347 adult female 01day    197 adult female 05day    58 adult female 30day    62 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0031424    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0031424 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0031424 expression level Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0031424 expression level Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    12.85 Larval Midgut    0.8 Larval Hindgut    2.4 Larval Malpighian Tubules    1.1 Larval Fat Body    1.9 Larval Salivary Gland    2 Larval Trachea    6.35 Larval Carcass    4.7 Adult Head no informative data Adult Eye    3.1 Adult Brain    65.2 Adult Thoracic-Abdominal Ganglion    44.9 Adult Crop    1.5 Adult Midgut    0.7 Adult Hindgut    2.1 Adult Malpighian Tubules    1.3 Adult Fat Body    8.9 Adult Salivary Gland    4.7 Adult Heart    0.975 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    1.5 Adult Testis    1.2 Adult Male Accessory Gland    6.2 Adult Carcass    4.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    3.1   NA  Brain    65.2   12.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    44.9   NA  Crop    1.5   0.8  Midgut    0.7   2.4  Hindgut    2.1   1.1  Malpighian Tubules    1.3   1.9  Fat Body    8.9   2  Salivary Gland    4.7   NA  Heart    0.975   6.35  Trachea    NA   NA  VirginFemale Spermatheca    2.8   NA  InseminatedFemale Spermatheca    1.1   NA  Ovary    1.5   NA  Testis    1.2   NA  Male Accessory Gland    6.2   4.7  Carcass    4.9 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_14
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031424 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9887-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4VGlut.PD VGlutScer\UAS.cDa VGlutScer\UAS.T:Avic\GFP-EGFP lethal | larval stage, with Scer\GAL4BG380 VGlutScer\UAS.cDa lethal | pupal stage, with Scer\GAL4VGlut.PD VGlutScer\UAS.T:Avic\GFP-EGFP viable, with Scer\GAL4pnr-MD237 VGlutGD834 viable, with Scer\GAL4VGlut.PD VGlutB1.Scer\UAS.T:Avic\GFP-EGFP VGlutB2.Scer\UAS.T:Avic\GFP-EGFP VGlutB3.Scer\UAS.T:Avic\GFP-EGFP VGlutB4.Scer\UAS.T:Avic\GFP-EGFP VGlutB5.Scer\UAS.T:Avic\GFP-EGFP VGlutB7.Scer\UAS.T:Avic\GFP-EGFP VGlutB9.Scer\UAS.T:Avic\GFP-EGFP VGlutB10.Scer\UAS VGlutB11.Scer\UAS.T:Avic\GFP-EGFP VGlutB13.Scer\UAS.T:Avic\GFP-EGFP VGlutB15.Scer\UAS.T:Avic\GFP-EGFP VGlutB16.Scer\UAS.T:Avic\GFP-EGFP VGlutB18.Scer\UAS.T:Avic\GFP-EGFP VGlutB19.Scer\UAS.T:Avic\GFP-EGFP VGlutB20.Scer\UAS.T:Avic\GFP-EGFP VGlutB21.Scer\UAS.T:Avic\GFP-EGFP VGlutB22.Scer\UAS.T:Avic\GFP-EGFP VGlutB23.Scer\UAS.T:Avic\GFP-EGFP VGlutB24.Scer\UAS.T:Avic\GFP-EGFP VGlutB25.Scer\UAS.T:Avic\GFP-EGFP VGlutB26.Scer\UAS.T:Avic\GFP-EGFP VGlutB27.Scer\UAS.T:Avic\GFP-EGFP VGlutB28.Scer\UAS.T:Avic\GFP-EGFP VGlutB29.Scer\UAS.T:Avic\GFP-EGFP VGlutB30.Scer\UAS.T:Avic\GFP-EGFP VGlutB31.Scer\UAS VGlutB32.Scer\UAS.T:Avic\GFP-EGFP VGlutB33.Scer\UAS.T:Avic\GFP-EGFP VGlutB34.Scer\UAS.T:Avic\GFP-EGFP VGlutB35.Scer\UAS VGlutB38.Scer\UAS.T:Avic\GFP-EGFP VGlutB39.Scer\UAS.T:Avic\GFP-EGFP VGlutB40.Scer\UAS.T:Avic\GFP-EGFP VGlutB41.Scer\UAS.T:Avic\GFP-EGFP VGlutB42.Scer\UAS.T:Avic\GFP-EGFP VGlutB43.Scer\UAS.T:Avic\GFP-EGFP VGlutB44.Scer\UAS.T:Avic\GFP-EGFP VGlutB45.Scer\UAS.T:Avic\GFP-EGFP VGlutB46.Scer\UAS.T:Avic\GFP-EGFP VGlutB47.Scer\UAS.T:Avic\GFP-EGFP VGlutB48.Scer\UAS.T:Avic\GFP-EGFP VGlutB49.Scer\UAS.T:Avic\GFP-EGFP VGlutB50.Scer\UAS.T:Avic\GFP-EGFP VGlutB52.Scer\UAS.T:Avic\GFP-EGFP VGlutRD7.Scer\UAS.T:Avic\GFP-EGFP VGlutRD16.Scer\UAS.T:Avic\GFP-EGFP Other Phenotypes Allele courtship behavior defective | male, with Scer\GAL4NP0225 VGlutScer\UAS.cDa courtship behavior defective | male, with Scer\GAL4αTub84B.PL VGlutScer\UAS.cDa flightless, with Scer\GAL4VGlut.PD VGlutScer\UAS.cDa gravitaxis behavior defective, with Scer\GAL4VGlut.PD VGlutScer\UAS.cDa neuroanatomy defective, with Scer\GAL4BG380 VGlutScer\UAS.cDa neuroanatomy defective, with Scer\GAL4VGlut.PD VGlutScer\UAS.T:Avic\GFP-EGFP neurophysiology defective VGlut1 neurophysiology defective, with Scer\GAL4BG380 VGlutScer\UAS.cDa neurophysiology defective, with Scer\GAL4elav-C155 VGlutScer\UAS.cDa neurophysiology defective, with Scer\GAL4VGlut.PD VGlutScer\UAS.cDa neurophysiology defective (with Df(2L)VGlut2) VGlut1 uncoordinated, with Scer\GAL4VGlut.PD VGlutScer\UAS.cDa Phenotype manifest in Allele antennal lobe, with Scer\GAL4VGlut.PD VGlutScer\UAS.T:Avic\GFP-EGFP embryonic/larval neuromuscular junction, with Scer\GAL4BG380 VGlutScer\UAS.cDa neuromuscular junction, with Scer\GAL4elav-C155 VGlutScer\UAS.cDa neuropil, with Scer\GAL4VGlut.PD VGlutScer\UAS.T:Avic\GFP-EGFP synaptic vesicle VGlut1 synaptic vesicle, with Scer\GAL4BG380 VGlutScer\UAS.cDa synaptic vesicle (with Df(2L)VGlut2) VGlut1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of VGlut Class Mutagen Stocks Known lesion VGlutKG06301 1 -- VGlutMB02384 1 -- VGlutMI02805 1 -- VGlutOK371 1 Yes VGlut1 Delta2-3 0 Yes VGlutOK371-dVP16AD recombination gene conversion 0 Yes
Alleles Carried on Transgenic Constructs ( 51 )
For All Alleles Carried on Transgenic Constructs Show
Allele of VGlut Class Mutagen Stocks Known lesion VGlutGD834 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes VGlutJF02689 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes VGlutKK107498 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes VGlutB1.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB10.Scer\UAS ethyl methanesulfonate 0 Yes VGlutB11.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB13.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB15.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB16.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB18.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB19.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB2.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB20.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB21.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB22.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB23.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB24.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB25.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB26.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB27.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB28.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB29.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB3.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB30.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB31.Scer\UAS ethyl methanesulfonate 0 Yes VGlutB32.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB33.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB34.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB35.Scer\UAS ethyl methanesulfonate 0 Yes VGlutB38.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB39.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB4.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB40.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB41.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB42.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB43.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB44.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB45.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB46.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB47.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB48.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB49.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB5.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB50.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB52.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB7.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutB9.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutRD16.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutRD7.Scer\UAS.T:Avic\GFP-EGFP ethyl methanesulfonate 0 Yes VGlutScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes VGlutScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7011 (Ryder, 2004.4.26)
Partially disrupted in	Df(2L)VGlut2 (Daniels et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD834} NA P{KK107498} NA P{TRiP.JF02689} NA P{UAS-VGlut.B1.GFP} NA P{UAS-VGlut.B2.GFP} NA P{UAS-VGlut.B3.GFP} NA P{UAS-VGlut.B4.GFP} NA P{UAS-VGlut.B5.GFP} NA P{UAS-VGlut.B7.GFP} NA P{UAS-VGlut.B9.GFP} NA P{UAS-VGlut.B10} NA P{UAS-VGlut.B11.GFP} NA P{UAS-VGlut.B13.GFP} NA P{UAS-VGlut.B15.GFP} NA P{UAS-VGlut.B16.GFP} NA P{UAS-VGlut.B18.GFP} NA P{UAS-VGlut.B19.GFP} NA P{UAS-VGlut.B20.GFP} NA P{UAS-VGlut.B21.GFP} NA P{UAS-VGlut.B22.GFP} NA P{UAS-VGlut.B23.GFP} NA P{UAS-VGlut.B24.GFP} NA P{UAS-VGlut.B25.GFP} NA P{UAS-VGlut.B26.GFP} NA P{UAS-VGlut.B27.GFP} NA P{UAS-VGlut.B28.GFP} NA P{UAS-VGlut.B29.GFP} NA P{UAS-VGlut.B30.GFP} NA P{UAS-VGlut.B31} NA P{UAS-VGlut.B32.GFP} NA P{UAS-VGlut.B33.GFP} NA P{UAS-VGlut.B34.GFP} NA P{UAS-VGlut.B35} NA P{UAS-VGlut.B38.GFP} NA P{UAS-VGlut.B39.GFP} NA P{UAS-VGlut.B40.GFP} NA P{UAS-VGlut.B41.GFP} NA P{UAS-VGlut.B42.GFP} NA P{UAS-VGlut.B43.GFP} NA P{UAS-VGlut.B44.GFP} NA P{UAS-VGlut.B45.GFP} NA P{UAS-VGlut.B46.GFP} NA P{UAS-VGlut.B47.GFP} NA P{UAS-VGlut.B48.GFP} NA P{UAS-VGlut.B49.GFP} NA P{UAS-VGlut.B50.GFP} NA P{UAS-VGlut.B52.GFP} NA P{UAS-VGlut.D} NA P{UAS-VGlut.GFP} NA P{UAS-VGlut.RD7.GFP} NA P{UAS-VGlut.RD16.GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}VGlutMI02805 NA P{SUPor-P}KG06301 NA insertion of enhancer trap binary system Mi{ET1}VGlutMB02384 No P{GawB}VGlutOK371 No insertion of enhancer trap P{dVP16AD}VGlutOK371-dVP16AD No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
neurotransmitter transporter activity inferred from mutant phenotype (Daniels et al., 2004)
Biological Process
CV term Evidence References
synaptic transmission, glutamatergic inferred from mutant phenotype (Daniels et al., 2006, Daniels et al., 2004)
Cellular Component
CV term Evidence References
synaptic vesicle inferred from direct assay (Daniels et al., 2004, Mahr and Aberle, 2006) vesicle inferred from direct assay (Shim et al., 2011)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
high affinity inorganic phosphate:sodium symporter activity inferred from sequence or structural similarity with UniProtKB:Q62634 (FlyBase, 1992-) L-glutamate transmembrane transporter activity inferred by curator from GO:0035249|GO:0008021 (Daniels et al., 2004) inferred from sequence alignment with human SLC17A7 (Mahr and Aberle, 2006, Daniels et al., 2004)
Biological Process
CV term Evidence References
L-glutamate transport inferred from sequence alignment with human SLC17A7 (Mahr and Aberle, 2006) transmembrane transport inferred from electronic annotation with InterPro:IPR011701 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR011701 (FlyBase Curators et al., 2004-) inferred from sequence model (Fei et al., 2007) inferred from sequence or structural similarity (Daniels et al., 2004) integral to synaptic vesicle membrane inferred by curator from GO:0008021|GO:0016021 (Daniels et al., 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions VGlut allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0031424 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33427
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15508 Dere\GG24856 Dgri\GH22431 Dmoj\GI15539 Dper\GL26556 Dpse\GA22101 Dsec\GM18340 Dsim\GD23154 Dvir\GJ17290 Dwil\GK23938 Dyak\GE18154
Curated drosophilid orthologs	Dpse\GA22101
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001867-PA Aedes aegypti (mosquito) AGAP007992-PA Anopheles gambiae (mosquito) XP_001943932.1 Acyrthosiphon pisum (pea aphid) 219926 Branchiostoma floridae (lancelet) BGIBMGA010643-PA Bombyx mori (silk worm) ENSBTAP00000009255 Bos taurus (bovine) CN14289 Caenorhabditis brenneri (small nematode) CBP01737 Caenorhabditis briggsae (nematode) CE01109 Caenorhabditis elegans (worm) ENSCAFP00000014842 Canis familiaris (dog) ENSCINP00000018357 Ciona intestinalis (sea squirt) JA08349 Caenorhabditis japonica (nematode) CPIJ000521 Culex pipens (mosquito) ENSCPOP00000006116 Cavia porcellus (guinea pig) RP43354 Caenorhabditis remanei (nematode) ENSCSAVP00000018768 Ciona savignyi (sedentary tunicate) 177109 Capitella spl (polychaete worm) DEHA2A07744g Debaryomyces Hansenii (yeast) DEHA2C12276g Debaryomyces Hansenii (yeast) DEHA2F00748g Debaryomyces Hansenii (yeast) DEHA2G24926g Debaryomyces Hansenii (yeast) 310221 Daphnia pulex (daphnia) ENSDARP00000090645 Danio rerio (zebra fish) ENSDARP00000090667 Danio rerio (zebra fish) ENSECAP00000022603 Equus caballus (horse) ENSGACP00000007800 Gymnopilus aculeatus (mushroom) ENSGACP00000017032 Gymnopilus aculeatus (mushroom) ENSGALP00000018888 Gallus gallus (chicken) ENSP00000221485 Homo sapiens (human) ISCW002570-PA Ixodes scapularis (deer tick) KLLA0C19019g Kluyveromyces lactis (yeast) 128007 Lottia gigantea (owl limpet) ENSMODP00000011627 Monodelphis domestica (gray opossum) ENSMMUP00000019427 Macaca mulatta (rhesus monkey) ENSMUSP00000082489 Mus musculus (house mouse) XP_001603704.1 Nasonia vitripennis (wasp) ENSOANP00000008879 Ornithorynchus anatinus (platypus) ENSORLP00000007275 Oryzias latipes (japanese killifish) PHUM003026-PA Pediculus humanus (human body louse) ENSPPYP00000005554 Pongo pygmaeus (orangutan) ENSPTRP00000009086 Pan troglodytes (chimpanzee) ENSRNOP00000028064 Rattus norvegicus (norway rat) Smp_129060 Schistosoma mansoni (trematode parasite) XP_786480.2 Strongylocentrotus purpuratus (purple sea urchin) XP_001813442.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012274 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000044264 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000018393 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15959 LH19015 ISCW002570 FBgn0235587 FBgn0225900 FBgn0204465 FBgn0194544 FBgn0173247 JGI_V11_310221 FBgn0082084 FBgn0164138 FBgn0138288 FBgn0129889 FBgn0117742 FBgn0116986 FBgn0092533 CPIJ000521 BGIBMGA010643 ACYPI20186 ACYPI000813 GB19507 AGAP007992 AAEL001867 PB22045 PHUM611550 GLEAN_08459
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	26160 w1118; P{GawB}VGlutOK371 23442 w1118; Mi{ET1}VGlutMB02384 27538 y1 v1; P{TRiP.JF02689}attP2 14146 y1 w67c23; P{SUPor-P}KG06301 36148 y1 w*; Mi{MIC}VGlutMI02805/SM6a
VDRC	v104324 P{KK107498}VIE-260B v2574 w1118; P{GD834}v2574/TM3
Genomic Clones ( 1 )
	BACR22P10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH74545
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH25247 RH26946 RH36156 RH43266 RH57669
Other clones	EC00353 EK029205 EK034721 EK040856 EK058124 EK107756 EK139445 EK162937 EK175217 EK181441 EK186503 EK196914 EK197147 EK200441 EK200633 EK202512 EK217356 EK218450 EK259223 EK284326 EK301839 EP02214 EP08364 EP18357
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0031424 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33427
Antibody Information
	polyclonal (Daniels et al., 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: VGlut CG9887 (Daniels et al., 2004)
Source for database merge of
Additional comments
Other Comments
	Increasing the expression of VGlut in motorneurons results in an increase in quantal size that is accompanied by an increase in synaptic vesicle volume. Despite the increased postsynaptic response to single vesicles, the response to evoked release of vesicles is unchanged because of a compensatory downregulation of presynaptic vesicle release. (Daniels et al., 2004)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_134837 RefSeq (Proteins) • NP_608681 DDBJ / EMBL / GenBank • AAF51256 AAF51256.1 AAO39443 BI607468 BT003440 Entrez Gene - A searchable database of RefSeq genes. • 33427 UniProtKB/TrEMBL • Q9VQC0 Q86P76
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Major facilitator superfamily (IPR011701) Major facilitator superfamily domain, general substrate transporter (IPR016196) Major facilitator superfamily domain (IPR020846)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0031424 DRSC - Results from RNAi screens. • FBgn0031424 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031424 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9887-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031424 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33427 InParanoid A subset of ortholog calls from InParanoid. • AAEL001867-PA Aedes aegypti (mosquito) AGAP007992-PA Anopheles gambiae (mosquito) XP_001943932.1 Acyrthosiphon pisum (pea aphid) 219926 Branchiostoma floridae (lancelet) BGIBMGA010643-PA Bombyx mori (silk worm) ENSBTAP00000009255 Bos taurus (bovine) CN14289 Caenorhabditis brenneri (small nematode) CBP01737 Caenorhabditis briggsae (nematode) CE01109 Caenorhabditis elegans (worm) ENSCAFP00000014842 Canis familiaris (dog) ENSCINP00000018357 Ciona intestinalis (sea squirt) JA08349 Caenorhabditis japonica (nematode) CPIJ000521 Culex pipens (mosquito) ENSCPOP00000006116 Cavia porcellus (guinea pig) RP43354 Caenorhabditis remanei (nematode) ENSCSAVP00000018768 Ciona savignyi (sedentary tunicate) 177109 Capitella spl (polychaete worm) DEHA2A07744g Debaryomyces Hansenii (yeast) DEHA2C12276g Debaryomyces Hansenii (yeast) DEHA2F00748g Debaryomyces Hansenii (yeast) DEHA2G24926g Debaryomyces Hansenii (yeast) 310221 Daphnia pulex (daphnia) ENSDARP00000090645 Danio rerio (zebra fish) ENSDARP00000090667 Danio rerio (zebra fish) ENSECAP00000022603 Equus caballus (horse) ENSGACP00000007800 Gymnopilus aculeatus (mushroom) ENSGACP00000017032 Gymnopilus aculeatus (mushroom) ENSGALP00000018888 Gallus gallus (chicken) ENSP00000221485 Homo sapiens (human) ISCW002570-PA Ixodes scapularis (deer tick) KLLA0C19019g Kluyveromyces lactis (yeast) 128007 Lottia gigantea (owl limpet) ENSMODP00000011627 Monodelphis domestica (gray opossum) ENSMMUP00000019427 Macaca mulatta (rhesus monkey) ENSMUSP00000082489 Mus musculus (house mouse) XP_001603704.1 Nasonia vitripennis (wasp) ENSOANP00000008879 Ornithorynchus anatinus (platypus) ENSORLP00000007275 Oryzias latipes (japanese killifish) PHUM003026-PA Pediculus humanus (human body louse) ENSPPYP00000005554 Pongo pygmaeus (orangutan) ENSPTRP00000009086 Pan troglodytes (chimpanzee) ENSRNOP00000028064 Rattus norvegicus (norway rat) Smp_129060 Schistosoma mansoni (trematode parasite) XP_786480.2 Strongylocentrotus purpuratus (purple sea urchin) XP_001813442.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012274 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000044264 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000018393 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33427 modMine - Data generated by the modENCODE project. • FBgn0031424 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15959 LH19015 ISCW002570 FBgn0235587 FBgn0225900 FBgn0204465 FBgn0194544 FBgn0173247 JGI_V11_310221 FBgn0082084 FBgn0164138 FBgn0138288 FBgn0129889 FBgn0117742 FBgn0116986 FBgn0092533 CPIJ000521 BGIBMGA010643 ACYPI20186 ACYPI000813 GB19507 AGAP007992 AAEL001867 PB22045 PHUM611550 GLEAN_08459
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	CG9887 (Daniels et al., 2004, Mahr and Aberle, 2006, Wheeler et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Mehnert and Cantera, 2008) DVGLUT (Daniels et al., 2004, Daniels et al., 2006, Mahr and Aberle, 2006, Collins et al., 2006, Krantz, 2006, Banovic et al., 2010, Wu et al., 2005, Beuchle et al., 2007, Koch et al., 2008, Hebbar et al., 2006, Wairkar et al., 2009, Wu et al., 2007, Leal et al., 2009, Séjourné et al., 2011, Viquez et al., 2006, Viquez et al., 2009, Grygoruk et al., 2010, Mehnert and Cantera, 2008, Kim et al., 2010, Xiong et al., 2010, Daniels et al., 2011) dvglut (Daniels et al., 2006, Krantz, 2006, Daniels et al., 2008, Daniels et al., 2011) DvGluT (Kolodziejczyk et al., 2008) DVGluT (Grosjean et al., 2008, Nojima et al., 2010) DVGlut (Brierley et al., 2009, Repnikova et al., 2010) dVGLUT (Shim et al., 2011) dvGluT (Kahsai and Winther, 2011) VGlut (Wheeler et al., 2006, Gao et al., 2008, Wheeler et al., 2008, Ranade et al., 2008, Marie et al., 2010, Hartwig et al., 2008, Stagg et al., 2011, Pack-Chung et al., 2007, Gao et al., 2008, Engström et al., 2007, Daniels et al., 2006, Shih and Chiang, 2011) VGluT (Bao et al., 2007) VGLUT (Fei et al., 2007, Tsurudome et al., 2010) vGluT (Johard et al., 2008, Nässel et al., 2008, Kolodziejczyk et al., 2008) vGLUT (Mohrmann et al., 2008) Vglut (Li et al., 2010)
Name Synonym	Drosophila vesicular glutamate transporter (Grosjean et al., 2008, Kim et al., 2010) Vesicular glutamate transporter (Daniels et al., 2004, Mahr and Aberle, 2006) vesicular glutamate transporter (Wheeler et al., 2006, Bao et al., 2007, Banovic et al., 2010, Johard et al., 2008, Pack-Chung et al., 2007, Nässel et al., 2008, Nojima et al., 2010, Kolodziejczyk et al., 2008, Kim et al., 2010, Tsurudome et al., 2010, Daniels et al., 2011)
Secondary FlyBase IDs
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 51 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 7 ) FlyBase analysis  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 19 )
	Chiang et al., 2011, Curr. Biol. 21(1): 1--11 Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution. [FBrf0212704] Daniels et al., 2011, Neurobiol. Disease 41(2): 415--420 Increased vesicular glutamate transporter expression causes excitotoxic neurodegeneration. [FBrf0212635] Das et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(36): E646--E654 Plasticity of local GABAergic interneurons drives olfactory habituation. [FBrf0215244] Kahsai and Winther, 2011, J. Comp. Neurol. 519(2): 290--315 Chemical neuroanatomy of the Drosophila central complex: distribution of multiple neuropeptides in relation to neurotransmitters. [FBrf0212649] Shih and Chiang, 2011, J. Neurogenet. 25(1-2): 1--6 Anatomical Characterization of Thermosensory AC Neurons in the Adult Drosophila Brain. [FBrf0213700] Shim et al., 2011, Biochem. J. 439(2): 277--286 l(2)01810 is a novel type of glutamate transporter that is responsible for megamitochondrial formation. [FBrf0216291] Stagg et al., 2011, Development 138(11): 2171--2183 Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate. [FBrf0213671] Séjourné et al., 2011, Nat. Neurosci. 14(7): 903--910 Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila. [FBrf0214029] Banovic et al., 2010, Neuron 66(5): 724--738 Drosophila Neuroligin 1 Promotes Growth and Postsynaptic Differentiation at Glutamatergic Neuromuscular Junctions. [FBrf0211035] de Haro et al., 2010, Cell Tissue Res. 339(2): 321--336 Detailed analysis of leucokinin-expressing neurons and their candidate functions in the Drosophila nervous system. [FBrf0209907] Grygoruk et al., 2010, J. Biol. Chem. 285(10): 6867--6878 A Tyrosine-based Motif Localizes a Drosophila Vesicular Transporter to Synaptic Vesicles in Vivo. [FBrf0210098] Kim et al., 2010, J. Cell Biol. 188(5): 717--734 The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. [FBrf0210177] Li et al., 2010, EMBO J. 29(5): 992--1006 Bicaudal-D binds clathrin heavy chain to promote its transport and augments synaptic vesicle recycling. [FBrf0210180] Marie et al., 2010, J. Neurosci. 30(24): 8071--8082 Synaptic homeostasis is consolidated by the cell fate gene gooseberry, a Drosophila pax3/7 homolog. [FBrf0211066] Nojima et al., 2010, Curr. Biol. 20(9): 836--840 Neuronal synaptic outputs determine the sexual fate of postsynaptic targets. [FBrf0210762] Repnikova et al., 2010, J. Neurosci. 30(18): 6466--6476 Sialyltransferase regulates nervous system function in Drosophila. [FBrf0210736] Tsurudome et al., 2010, Neuron 68(5): 879--893 The Drosophila miR-310 Cluster Negatively Regulates Synaptic Strength at the Neuromuscular Junction. [FBrf0212495] Wu et al., 2010, Nat. Neurosci. 13(1): 69--75 SLEEPLESS, a Ly-6/neurotoxin family member, regulates the levels, localization and activity of Shaker. [FBrf0209634] Xiong et al., 2010, J. Cell Biol. 191(1): 211--223 Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury. [FBrf0211939] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...