FlyBase Gene Report: Dmel\VGlut1

adult insulin secreting cell of pars intercerebralis | subset

(Cavanaugh et al., 2014)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

lamina monopolar neuron L1

(Takemura et al., 2011)

(Karuppudurai et al., 2014)

embryonic/larval neuromuscular junction

(Karuppudurai et al., 2014)

Additional Descriptive Data

Expression of VGlut1 was established via single cell RNA sequencing of ScerGAL4^Kurs58 or ScerGAL4^c767 positive pars intercerebralis neurons. Expression was found in four out of five tested cells.

(Cavanaugh et al., 2014)

RT-PCR analysis on isolated GFP-labeled transmedullary neurons Tm5c and distal medullary amacrine neurons Dm8 found high levels of VGlut1 transcripts.

(Karuppudurai et al., 2014)

Strong expression of VGlut1 is seen in cells similar to alpha-processes of the amacrine neurons or possibly like beta-processes of T1 neurons. Weak VGlut1 expression is seen in cell bodies in the chiasma between lamina and medulla in a position corresponding to the amacrine cells.

(Kolodziejczyk et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

(Banovic et al., 2010)

first instar larval stage

larval ventral nerve cord | subset

• presynaptic active zone

Comment: glutamatergic

(Stacey et al., 2010)

adult ellipsoid body ring neuron

adult optic lobe

(Daniels et al., 2008)

medulla

(Daniels et al., 2008)

lobula

(Daniels et al., 2008)

lobula plate

(Daniels et al., 2008)

adult antennal lobe

(Daniels et al., 2008)

adult central complex

(Daniels et al., 2008)

fan-shaped body

(Daniels et al., 2008)

nodulus

(Daniels et al., 2008)

ellipsoid body

(Daniels et al., 2008)

(Daniels et al., 2008)

protocerebral bridge

(Daniels et al., 2008)

adult gnathal ganglion

(Daniels et al., 2008)

superior medial protocerebrum

(Daniels et al., 2008)

(Daniels et al., 2008)

ventrolateral protocerebrum

(Daniels et al., 2008)

mushroom body spur | faint

(Daniels et al., 2008)

adult mushroom body alpha-lobe | faint

(Daniels et al., 2008)

mushroom body calyx | faint

(Daniels et al., 2008)

adult mushroom body gamma-lobe | faint

(Daniels et al., 2008)

lamina monopolar neuron L1

(Karuppudurai et al., 2014)

• axon terminus

(Richter et al., 2018)

medulla intrinsic neuron Mi9

• axon terminus

(Richter et al., 2018)

lobula plate intrinsic neuron LPi4-3

• axon terminus

(Richter et al., 2018)

(Karuppudurai et al., 2014)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

VGlut1 protein is expressed in puncta and segmentally repeated in the ventral nerve cord, labeling the glutamatergic presynaptic terminals. The marked cells include the longitudinal (interface) glial cells, where VGlut1 protein is found adjacent to ScerGAL4^Eaat1.PR.

(Stacey et al., 2010)

Expression of VGlut1 protein is seen extensively throughout the central nervous system of the third instar larval brain, with approximately 200 in each brain lobe and 800 in the ventral nerve cord. High VGlut1 protein expression is reported to be found within the following larval brain compartments: dorsal compartments - dorsoanterior, dorsoposterior, and centroposterior-lateral (dorsal); central compartments - centroanterior, centroposterior-lateral, centroposterior- medial, and centroposterior-intermediate; and basal compartments - centroanterior (ventral), basoanterior, basocentral, basocervical, basoposterior-lateral, and basoposterior-medial (BPM). Highest expression is found in the dorsoanterior and centroanterior compartments. Although VGlut1 protein is generally not expressed in the larval mushroom body, a small number of punctae are seen in the vertical lobes, medial lobes and the spur. Staining within the ventral nerve cord is predominantly on the dorsal and lateral surface and is largely excluded from the ventral aspect of the neuropil. In the adult brain, expression of VGlut1 but shows extensive expression in central neuropil compartments, but is predominantly absent from the mushroom body only being detectable as punctae in the base of the gamma-lobe, the tip and base of the alpha-lobe, the spur, and faintly in the calyx. Expression within the ellipsoid body is observed within its inner and outer layers, a pattern suggestive of R-cell innervation. Expression of VGlut1 is observed in three prominent layers of the medulla - two in the outer medulla, and one encompassing the inner medulla.

(Daniels et al., 2008)

Marker for

motor neuron

(Touma et al., 2012)

Subcellular Localization

CV Term

Evidence

References

located_in synaptic vesicle membrane

inferred from direct assay

(Daniels et al., 2004)

located_in synaptic vesicle

inferred from direct assay

(Mahr and Aberle, 2006)

located_in vesicle

inferred from direct assay

(Shim et al., 2011)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.2}VGlut1^{MI04979-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

(Diao et al., 2015)

(Diao et al., 2015)

larval DA1 motor neuron

(Diao et al., 2015)

larval RP2 motor neuron

(Diao et al., 2015)

DN1 neuron | subset

(Guo et al., 2016)

medulla intrinsic neuron Mi9

(Hill et al., 2019, Lacin et al., 2019)

(Davis et al., 2020)

adult glutamatergic neuron

(McKinney et al., 2020)

adult octopaminergic neuron | subset

(McKinney et al., 2020)

Reporter: Mi{Trojan-GAL4DBD.2}VGlut1^{MI04979-TG4DBD.2}

Stage

Tissue/Position (including subcellular localization)

Reference

U neuron

Comment: when combined with TI{2A-p65(AD)::Zip+}unc-4^AD

(Lacin et al., 2020)

octopaminergic neuron | subset

Comment: when combined with P{Tdc2-p65.AD}

(Sherer et al., 2020)

adult motor neuron of neck

Comment: when combined with TI{2A-p65(AD)::Zip+}unc-4^AD

(Lacin et al., 2020)

DLM motor neuron

Comment: when combined with TI{2A-p65(AD)::Zip+}unc-4^AD

(Lacin et al., 2020)

adult lateral horn neuron | subset

Comment: when combined with P{R23C09-p65.AD}

(Varela et al., 2019)

adult neuron | subset of adult brain

Comment: when combined with P{R23C09-p65.AD}

(Varela et al., 2019)

adult glutamatergic neuron | subset

Comment: when combined with Mi{Trojan-GAL4DBD.2}VGlut^{MI04979-TG4DBD.2} (combination referred to as 'Tdc2/vGlut')

(McKinney et al., 2020)

adult octopaminergic neuron | subset

Comment: when combined with Mi{Trojan-GAL4DBD.2}VGlut^{MI04979-TG4DBD.2} (combination referred to as 'Tdc2/vGlut')

(McKinney et al., 2020)

adult tyraminergic neuron | subset

Comment: when combined with Mi{Trojan-GAL4DBD.2}VGlut^{MI04979-TG4DBD.2} (combination referred to as 'Tdc2/vGlut')

(McKinney et al., 2020)

Reporter: Mi{Trojan-lexA:QFAD.2}VGlut1^{MI04979-TlexA:QFAD.2}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

(Diao et al., 2015)

first instar larval stage

(Diao et al., 2015)

glutamatergic neuron | subset of larval ventral nerve cord

(Babski et al., 2019)

wandering third instar larval stage

organism

(Takagi et al., 2017)

pupal stage P6 -- pharate adult stage P15

motor neuron

(Constance et al., 2018)

glutamatergic local interneuron of the adult antennal lobe

(Coates et al., 2017)

Reporter: Mi{Trojan-QF2.2}VGlut1^{MI04979-TQF2.2}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

(Diao et al., 2015)

wandering third instar larval stage

(Diao et al., 2015)

(Venkatasubramanian et al., 2019)

(Venkatasubramanian et al., 2019)

pupal stage -- adult stage

(Venkatasubramanian et al., 2019)

motor neuron

(Venkatasubramanian et al., 2019)

leg muscle motor neuron

(Venkatasubramanian et al., 2019)

adult ellipsoid body extrinsic ring neuron

(Xie et al., 2017)

small field neuron of the central complex | restricted

(Xie et al., 2017)

glutamatergic neuron of wing

(Szabó et al., 2023)

wing nerve

(Szabó et al., 2023)

adult female pheromone-sensing neuron of leg

(Kallman et al., 2015)

Reporter: P{dVP16AD}VGlut1^{OK371-dVP16AD}

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8 -- P15

Comment: when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014)

lamina monopolar neuron L1

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Bahl et al., 2015, Gao et al., 2008)

adult optic lobe neuron | subset

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-Gal4.C1a.DBD}

when combined with P{ort-Gal4.C1a.DBD} (combination referred to as 'ortC1a∩ ok371-dVP16')

(Sancer et al., 2019, Melnattur et al., 2014, Gao et al., 2008)

Comment: when combined with P{ort-GAL4.DBD.C1-3}

(Gao et al., 2008)

distal medullary amacrine neuron Dm8a | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

glial cell | subset

Comment: when combined with P{ort-GAL4.DBD.C2}

(Gao et al., 2008)

adult doublesex aDN neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

when combined with TI{GAL4(DBD)::Zip-}dsx^GAL4-DBD

(Nojima et al., 2021, Pavlou et al., 2016)

glutamatergic neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

motor neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

prothoracic leg taste bristle chemosensory neuron | subset

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult glutamatergic doublesex AbN (female) neuron

Comment: when combined with P{ort-GAL4.DBD.C1-3}

when combined with P{ort-GAL4.DBD.C2}

(Ting et al., 2014, Gao et al., 2008)

adult stage | female

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

adult stage | male

adult glutamatergic doublesex AbN (male) neuron

Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4-DBD

(Pavlou et al., 2016)

larval protocerebrum | restricted

Comment: when combined with P{ort-LexA.C1a.DBD}

(Karuppudurai et al., 2014)

Reporter: P{GawB}VGlut1^OK371

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

(Nässel et al., 2008)

adult mushroom body beta-lobe | surrounding

(Nässel et al., 2008)

larval ventral nerve cord

(Nässel et al., 2008)

medial lobe of larval mushroom body

(de Haro et al., 2010)

(Johard et al., 2008)

spur of larval mushroom body

(Johard et al., 2008)

embryonic/larval mushroom body

(Johard et al., 2008)

larval prothoracic neuromere of central nervous system

(Brierley et al., 2012)

larval glutamatergic neuron

(Pogodalla et al., 2021)

third instar larval stage -- P-stage

motor neuron of prothoracic leg

(Brierley et al., 2012)

Reporter: P{VGlut1-Gal4.6780}

Stage

Tissue/Position (including subcellular localization)

Reference

transmedullary neuron Tm9

(Ting et al., 2014)

Reporter: P{VGlut1-GAL4.D}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic/larval neuromuscular junction of glutamatergic neuron

(Daniels et al., 2008)

embryonic/larval neuromuscular junction | restricted

Comment: Presynaptic

(Graf et al., 2009)

wandering third instar larval stage

glutamatergic neuron of larval ventral nerve cord

(Daniels et al., 2008)

embryonic/larval optic lobe

(Daniels et al., 2008)

adult mushroom body alpha-lobe | faint

mushroom body spur | faint

(Daniels et al., 2008)

(Daniels et al., 2008)

mushroom body calyx | faint

(Daniels et al., 2008)

adult antennal lobe

(Daniels et al., 2008)

adult antennal lobe local neuron | subset

(Daniels et al., 2008)

adult antennal lobe projection neuron | subset

(Daniels et al., 2008)

motor neuron | subset

(Daniels et al., 2008)

ellipsoid body

Comment: strong expression

(Chen et al., 2012, Daniels et al., 2008)

adult ellipsoid body ring neuron

(Daniels et al., 2008)

fan-shaped body

(Kahsai et al., 2012, Daniels et al., 2008)

nodulus

(Daniels et al., 2008)

protocerebral bridge

(Daniels et al., 2008)

protocerebral bridge glomerulus 9 | faint

(Daniels et al., 2008)

adult optic lobe

(Daniels et al., 2008)

medulla

(Daniels et al., 2008)

lobula plate

(Daniels et al., 2008)

superior medial protocerebrum

(Daniels et al., 2008)

ventrolateral protocerebrum

(Daniels et al., 2008)

adult gnathal ganglion

(Daniels et al., 2008)

adult lateral horn

(Daniels et al., 2008)

antennal lobe projection neuron

(Wu et al., 2010)

neuron | subset of adult brain

(Chen et al., 2012)

adult mushroom body

Comment: faint expression

(Chen et al., 2012)

eye

Comment: medium expression

(Chen et al., 2012)

Comment: faint expression

(Chen et al., 2012)

lamina | restricted

(Raghu and Borst, 2011)

medulla | restricted

(Raghu and Borst, 2011)

lobula | restricted

(Raghu and Borst, 2011)

lobula plate | restricted

(Raghu and Borst, 2011)

lamina monopolar neuron L2

(Raghu and Borst, 2011)

transmedullary neuron Tm9

(Raghu and Borst, 2011)

transmedullary neuron Tm20

(Raghu and Borst, 2011)

transmedullary neuron vGlutTmnew1

(Raghu and Borst, 2011)

transmedullary Y neuron vGlutTmYnew1

(Raghu and Borst, 2011)

Y neuron Y1

(Raghu and Borst, 2011)

Y neuron vGlutYnew1

(Raghu and Borst, 2011)

medulla intrinsic neuron Mi4

(Raghu and Borst, 2011)

medulla intrinsic neuron vGlutMinew1

(Raghu and Borst, 2011)

medulla intrinsic neuron vGlutMinew2

(Raghu and Borst, 2011)

distal medullary amacrine neuron Dm3

(Raghu and Borst, 2011)

distal medullary amacrine neuron Dm5

(Raghu and Borst, 2011)

distal medullary amacrine neuron vGlutDmnew1

(Raghu and Borst, 2011)

proximal medullary amacrine neuron Pm2

(Raghu and Borst, 2011)

medulla intrinsic amacrine neuron vGlutMi Amnew1

(Raghu and Borst, 2011)

medulla tangential neuron Mt11

(Raghu and Borst, 2011)

T3 neuron

(Raghu and Borst, 2011)

T5 neuron

(Raghu and Borst, 2011)

translobula plate neuron Tlp1

(Raghu and Borst, 2011)

translobula plate neuron Tlp3

(Raghu and Borst, 2011)

translobula plate neuron vGlutTlpnew1

(Raghu and Borst, 2011)

lobula columnar neuron LC4

(Raghu and Borst, 2011)

lobula columnar neuron LC5

(Raghu and Borst, 2011)

lobula columnar neuron LC8

(Raghu and Borst, 2011)

lobula tangential neuron LT4

(Raghu and Borst, 2011)

lobula tangential neuron LT6

(Raghu and Borst, 2011)

lobula intrinsic neuron Li1

(Raghu and Borst, 2011)

ventral giant horizontal cell HSS

(Raghu and Borst, 2011)

lobula plate intrinsic neuron

(Raghu and Borst, 2011)

ellipsoid body outer central domain | restricted

(Kahsai et al., 2012)

ellipsoid body outer posterior domain | restricted

(Kahsai et al., 2012)

protocerebral bridge | restricted

(Kahsai et al., 2012)

bulb | restricted

(Kahsai et al., 2012)

nodulus | restricted

(Kahsai et al., 2012)

adult mushroom body gamma-lobe | faint

(Daniels et al., 2008)

Reporter: P{VGlut1-GAL4.U}

Stage

Tissue/Position (including subcellular localization)

Reference

medulla non-directional M6 local neuron 3

(Chin et al., 2014)

medulla non-directional M6 local neuron 4

(Chin et al., 2014)

medulla directional M6 local neuron

(Chin et al., 2014)

Reporter: P{VGlut1-GAL4.VGN6341}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{VGlut1-GAL4.VGN9281}

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P15(i)

motor neuron | subset

(Brierley et al., 2009)

interneuron | subset

(Brierley et al., 2009)

motor neuron | subset of tibia

(Syed et al., 2016)

Reporter: P{VGlut1-lexA.M}

Stage

Tissue/Position (including subcellular localization)

Reference

• neuromuscular junction

(Shearin et al., 2013)

adult female pheromone-sensing neuron of leg

(Kallman et al., 2015)

Reporter: P{VGlut1-lexA.S}

Stage

Tissue/Position (including subcellular localization)

Reference

(Tastekin et al., 2018)

Reporter: TI{2A-lexA::GAD}VGlut1^2A-lexA

Stage

Tissue/Position (including subcellular localization)

Reference

anterior-projecting DN3 neuron

glutamatergic neuron | subset

(Deng et al., 2019)

(Sun et al., 2022)

adult glutamatergic neuron

(Sun et al., 2022)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\VGlut1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\VGlut1

View exonic expression by tissue for Dmel\VGlut1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33427/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031424

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031424

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031424

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9887

Flygut - An atlas of the Drosophila adult midgut

FBgn0031424

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 44 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 58 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of VGlut1

Transgenic constructs containing regulatory region of VGlut1

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)Exel7011

(Ryder, 2004.4.26)

Gene partially disrupted in

Df(2L)VGlut1²

(Daniels et al., 2006)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

VGlut1^{MI04979-GFSTF.2}

lethal, with Scer\GAL4^VGlut1.PD

lethal - all die before end of larval stage, with Scer\GAL4^BG380

VGlut1^UAS.cDa

lethal - all die before end of pupal stage, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.EGFP

viable

VGlut1^MI04979

viable, with Scer\GAL4^Mef2.PR

VGlut1^GD834

viable, with Scer\GAL4^pnr-MD237

VGlut1^GD834

viable, with Scer\GAL4^tin.CΔ4

VGlut1^GD834

Sterility

Allele

female semi-sterile, with Scer\GAL4^Ilp7.PC

VGlut1^JF02689

fertile, with Scer\GAL4^Tdc2.PC

VGlut1^JF02689

Other Phenotypes

Allele

abnormal courtship behavior | male, with Scer\GAL4^NP0225

VGlut1^UAS.cDa

abnormal courtship behavior | male, with Scer\GAL4^αTub84B.PL

VGlut1^UAS.cDa

abnormal locomotor behavior | adult stage, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.cDa

abnormal locomotor behavior | larval stage, with Scer\GAL4^per.PK

VGlut1^HMS02011

abnormal memory | adult stage, with Scer\GAL4^GMR27G01

VGlut1^KK107498

abnormal memory | adult stage, with Scer\GAL4^VT000765

VGlut1^KK107498

abnormal memory | adult stage, with Scer\GAL4^VT000765, Scer\GAL4^VT041043

VGlut1^KK107498

abnormal memory | adult stage, with Scer\GAL4^VT041043

VGlut1^KK107498

abnormal neuroanatomy, with Scer\GAL4^BG380

VGlut1^UAS.cDa

abnormal neuroanatomy, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.EGFP

abnormal neurophysiology

VGlut1¹

abnormal neurophysiology, with Scer\GAL4^BG380

VGlut1^UAS.cDa

abnormal neurophysiology, with Scer\GAL4^elav-C155

VGlut1^UAS.cDa

abnormal neurophysiology, with Scer\GAL4^Toll-6-D42

VGlut1^UAS.cDa

abnormal neurophysiology, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.cDa

abnormal neurophysiology (with Df(2L)VGlut1²)

VGlut1¹

abnormal neurophysiology | adult stage (with Df(2L)VGlut1²)

VGlut1^-B3RT

abnormal neurophysiology | larval stage, with Scer\GAL4^RapGAP1-OK6, Scer\GAL4^Toll-6-D42

VGlut1^HMS02011

abnormal neurophysiology | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

abnormal phototaxis, with Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut1-OK371}

VGlut1^JF02689

abnormal phototaxis, with Scer\GAL4^ort.C2b

VGlut1^JF02689

decreased rate of movement | adult stage, with Scer\GAL4^ple.PF

VGlut1^HMS02011

decreased size | adult stage (with Df(2L)VGlut1²)

VGlut1^-B3RT

flightless, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.cDa

increased rate of movement | adult stage, with Scer\GAL4^ple.PF

VGlut1^UAS.cDa

some die during larval stage, with Scer\GAL4^BG380

VGlut1^UAS.cDa

some die during pupal stage, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.EGFP

uncoordinated, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.cDa

Phenotype manifest in

Allele

adult motor neuron (with Df(2L)VGlut1²)

VGlut1^-B3RT

antennal lobe, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.EGFP

embryonic/larval neuromuscular junction, with Scer\GAL4^BG380

VGlut1^UAS.cDa

embryonic/larval neuromuscular junction, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

motor neuron | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

muscle cell of A1-7 ventral longitudinal muscle 3, with Scer\GAL4^RapGAP1-OK6, Scer\GAL4^Toll-6-D42

VGlut1^HMS02011

muscle cell of A1-7 ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

neuromuscular junction, with Scer\GAL4^elav-C155

VGlut1^UAS.cDa

neuromuscular junction, with Scer\GAL4^Toll-6-D42

VGlut1^UAS.cDa

neuropil, with Scer\GAL4^VGlut1.PD

VGlut1^UAS.EGFP

NMJ bouton | adult stage (with Df(2L)VGlut1²)

VGlut1^-B3RT

presynapse | adult stage (with Df(2L)VGlut1²)

VGlut1^-B3RT

presynaptic active zone | adult stage (with Df(2L)VGlut1²)

VGlut1^-B3RT

synaptic vesicle

VGlut1¹

synaptic vesicle, with Scer\GAL4^BG380

VGlut1^UAS.cDa

synaptic vesicle (with Df(2L)VGlut1²)

VGlut1¹

type Ib motor neuron | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

type Is motor neuron | larval stage, with Scer\GAL4^VGlut1-OK371

VGlut1^UAS.cDa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (13)

Hsap\SLC17A6

13 of 14

Yes

Hsap\SLC17A7

12 of 14

Yes

Hsap\SLC17A8

12 of 14

Yes

6 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (14)

Rnor\Slc17a6

13 of 14

Yes

Rnor\Slc17a7

12 of 14

Yes

Rnor\Slc17a8

12 of 14

Yes

6 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Slc17a6

12 of 14

Yes

Mmus\Slc17a7

12 of 14

Yes

Mmus\Slc17a8

11 of 14

Yes

6 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (9)

Xtro\slc17a7

10 of 13

Yes

Xtro\slc17a6

7 of 13

Yes

Xtro\slc17a8

7 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (16)

Drer\slc17a6a

13 of 14

Yes

Drer\slc17a6b

12 of 14

Yes

Drer\slc17a7a

12 of 14

Yes

Drer\slc17a7b

12 of 14

Yes

Drer\slc17a8

12 of 14

Yes

Drer\slc17a5

6 of 14

Drer\si:ch1073-513e17.1

5 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (53)

Cele\eat-4

12 of 14

Yes

Cele\vglu-2

6 of 14

Yes

Cele\vglu-3

6 of 14

Yes

5 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP007992

11 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\ANTR2

11 of 13

Yes

Atha\PHT4;1

11 of 13

Yes

Atha\PHT4;2

10 of 13

Yes

Atha\PHT4;3

10 of 13

Yes

Atha\PHT4;5

10 of 13

Yes

Atha\PHT4;6

7 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\TNA1

3 of 13

Yes

Scer\DAL5

2 of 13

Scer\SOA1

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\liz1

3 of 12

Yes

Spom\SPAC11D3.18c

3 of 12

Yes

Spom\SPAC1002.16c

3 of 12

Yes

Spom\SPAC1039.04

3 of 12

Yes

Spom\SPBC1683.12

3 of 12

Yes

Spom\SPBC1773.15

3 of 12

Yes

Spom\SPBPB10D8.01

3 of 12

Yes

Spom\SPCC417.10

3 of 12

Yes

Spom\SPCC757.13

3 of 12

Yes

Spom\SPCPB1C11.03

3 of 12

Yes

Spom\SPAC1B3.15c

1 of 12

Yes

Spom\vht1

1 of 12

Yes

Escherichia coli (enterobacterium) (6)

Ecol\dgoT

7 of 11

Yes

Ecol\gudP

7 of 11

Yes

6 of 11

6 of 11

4 of 11

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:VGlut1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

9 of 13

9 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

6 of 13

5 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

VGlut1^UAS.cDa

model of Parkinson's disease

CEA

(Steinkellner et al., 2018)

model of neurodegenerative disease

CEA

(Steinkellner et al., 2018, Daniels et al., 2011)

VGlut1^B44.UAS.EGFP

DOES NOT model neurodegenerative disease

CEA

(Daniels et al., 2011)

VGlut1¹

model of Kohlschutter-Tonz syndrome

CEA with Indy³⁰²

(Jeong et al., 2021)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SLC17A8; solute carrier family 17 member 8

model of autosomal dominant nonsyndromic deafness 25

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

VGlut1^UAS.cDa

ameliorates Kohlschutter-Tonz syndrome

modeled by Indy^NIG.3979R

(Jeong et al., 2021)

modeled by Indy^GD2712

(Jeong et al., 2021)

modeled by Indy^{T245M.B.UAS.Tag:V5}

(Jeong et al., 2021)

VGlut1^GD834

ameliorates neurodegenerative disease

modeled by mir-1000^KO2, mir-1000^KO2-GAL4

(Verma et al., 2015)

VGlut1^JF02689

exacerbates Parkinson's disease

modeled by Hsc70-5^KK100233

(Zhu et al., 2021)

VGlut1^HMS02011

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^36.UAS.EGFP

(Xu and Xu, 2018)

VGlut1^HMS02175

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^36.UAS.EGFP

(Xu and Xu, 2018)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC17A6; solute carrier family 17 member 6

13 of 14

607563

SLC17A7; solute carrier family 17 member 7

12 of 14

605208

SLC17A8; solute carrier family 17 member 8

12 of 14

607557

DEAFNESS, AUTOSOMAL DOMINANT 25; DFNA25

autosomal dominant nonsyndromic deafness 25

SLC17A5; solute carrier family 17 member 5

6 of 14

604322

sialuria

SLC17A2; solute carrier family 17 member 2

4 of 14

611049

SLC17A3; solute carrier family 17 member 3

4 of 14

611034

URIC ACID CONCENTRATION, SERUM, QUANTITATIVE TRAIT LOCUS 4; UAQTL4

SLC17A1; solute carrier family 17 member 1

3 of 14

182308

SLC17A4; solute carrier family 17 member 4

3 of 14

604216

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

VGlut1 - lap

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Yu et al., 2020, Vanlandingham et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Li et al., 2021)

enhanceable

(Kiragasi et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Eaat1

suppressible

(Peng et al., 2019)

suppressible

(Han et al., 2023)

enhanceable

(Han et al., 2023)

enhanceable

(Zhu et al., 2021)

suppressible

(Hu et al., 2015)

KaiR1D

enhanceable

(Kiragasi et al., 2017)

mir-1000

suppressible

(Verma et al., 2015)

Mon1

suppressible

(Deivasigamani et al., 2015)

TrpA1

suppressible