protein is expressed in puncta and segmentally repeated in the ventral nerve cord, labeling the glutamatergic presynaptic terminals. The marked cells include the longitudinal (interface) glial cells, where VGlut
protein is found adjacent to Scer\GAL4Eaat1.PR
Expression of VGlut
protein is seen extensively throughout the central nervous system of the third instar larval brain, with approximately 200 in each brain lobe and 800 in the ventral nerve cord. High VGlut
protein expression is reported to be found within the following larval brain compartments: dorsal compartments - dorsoanterior, dorsoposterior, and centroposterior-lateral (dorsal); central compartments - centroanterior, centroposterior-lateral, centroposterior- medial, and centroposterior-intermediate; and basal compartments - centroanterior (ventral), basoanterior, basocentral, basocervical, basoposterior-lateral, and basoposterior-medial (BPM). Highest expression is found in the dorsoanterior and centroanterior compartments. Although VGlut
protein is generally not expressed in the larval mushroom body, a small number of punctae are seen in the vertical lobes, medial lobes and the spur. Staining within the ventral nerve cord is predominantly on the dorsal and lateral surface and is largely excluded from the ventral aspect of the neuropil. In the adult brain, expression of VGlut
but shows extensive expression in central neuropil compartments, but is predominantly absent from the mushroom body only being detectable as punctae in the base of the gamma-lobe, the tip and base of the alpha-lobe, the spur, and faintly in the calyx. Expression within the ellipsoid body is observed within its inner and outer layers, a pattern suggestive of R-cell innervation. Expression of VGlut
is observed in three prominent layers of the medulla - two in the outer medulla, and one encompassing the inner medulla.