FB2020_01, released Feb 12, 2020

Gene: Dmel\Hrs

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General Information
Symbol
Dmel\Hrs
Species
D. melanogaster
Name
Hepatocyte growth factor regulated tyrosine kinase substrate
Annotation Symbol
CG2903
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031450
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Snapshot
Hepatocyte growth factor regulated tyrosine kinase substrate (Hrs) encodes a protein that, together with the product encoded by Stam, forms the ESCRT-0 complex. This complex is responsible for the initial selection of ubiquitinated proteins that are ultimately degraded in lysosomes. [Date last reviewed: 2018-10-04]
Other Summaries
Also Known As

l(2)23Ad, l(2)23AB5, vps27

Key Links
Genomic Location
Cytogenetic map
23A3-23A3
Sequence location
2L:2,739,986..2,743,377 [+]
Recombination map

2-7

RefSeq locus
NT_033779 REGION:2739986..2743377
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
ubiquitin binding
Predictions / Assertions
metal ion binding
Summaries
Gene Group (FlyBase)
ESCRT-0 COMPLEX -
The ESCRT (Endosomal Sorting Complexes Required for Transport) machinery is made up of five complexes, ESCRT-0 to -III and the Vps4-Vta1 ATPase complex, which are sequentially recruited to the cytosolic face of the membrane, and mediate cargo recognition, membrane budding and fission. ESCRT-0 initiates mediates multivesicular body (MVB) formation, binding ubiquitinated membrane proteins destined for degradation and concentrating them in early endosome membranes. ESCRT-0 recruits ESCRT-I. (Adapted from PMID:22361144 and FBrf0209668).
Pathway (FlyBase)
Negative Regulators of Torso Signaling Pathway -
Negative regulators of Torso signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl).
Positive Regulators of Toll-NF-KappaB Signaling Pathway -
In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
Protein Function (UniProtKB)
Essential role in endosome membrane invagination and formation of multivesicular bodies, MVBs. Required during gastrulation and appears to regulate early embryonic signaling pathways. Inhibits tyrosine kinase receptor signaling by promoting degradation of the tyrosine-phosphorylated, active receptor, potentially by sorting activated receptors into MVBs. The MVBs are then trafficked to the lysosome where their contents are degraded.
(UniProt, Q960X8)
Summary (Interactive Fly)

endosomal sorting of ubiquitinated receptors - targeting of EGFR, PVR, Notch, Hedgehog and Dpp receptors for degradation

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Hrs or the JBrowse view of Dmel\Hrs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Hrs-RA
FBtr0089749
NM_164497
2251
647
Hrs-RB
FBtr0089750
NM_080360
2714
760
Hrs-RC
FBtr0089751
NM_164496
2665
760
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Hrs-PA
FBpp0088690
72.4
647
6.21
NP_722831
AAF51222
Hrs-PB
FBpp0088691
85.4
760
6.97
NP_525099
AAF51221
Hrs-PC
FBpp0088692
85.4
760
6.97
NP_722830
AAN10412
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

760 aa isoforms: Hrs-PB, Hrs-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homodimer; in vitro. Interacts with ubiquitin.

(UniProt, Q960X8)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hrs using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (20 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
ubiquitin binding
inferred from direct assay
(Lloyd et al., 2002)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
metal ion binding
inferred from electronic annotation with InterPro:IPR000306
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (17 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
early endosome to late endosome transport
inferred from mutant phenotype
(Pradhan-Sundd and Verheyen, 2014)
endocytic recycling
inferred from mutant phenotype
(Pradhan-Sundd and Verheyen, 2014)
endocytosis
inferred from mutant phenotype
(Rives et al., 2006)
endosomal transport
inferred from mutant phenotype
(Sheng et al., 2016, Chanut-Delalande et al., 2010)
endosome transport via multivesicular body sorting pathway
inferred from mutant phenotype
(Lloyd et al., 2002)
exosomal secretion
inferred from mutant phenotype
(Corrigan et al., 2014)
negative regulation of protein phosphorylation
inferred from direct assay
(Fan et al., 2013)
negative regulation of torso signaling pathway
inferred from mutant phenotype
(Lloyd et al., 2002)
neuron remodeling
inferred from genetic interaction with FLYBASE:Stam; FB:FBgn0027363
(Issman-Zecharya and Schuldiner, 2014)
positive regulation of border follicle cell migration
inferred from mutant phenotype
(Jekely et al., 2005)
positive regulation of protein ubiquitination
inferred from mutant phenotype
(Fan et al., 2013)
positive regulation of Toll signaling pathway
inferred from mutant phenotype
(Huang et al., 2010)
positive regulation of Wnt protein secretion
inferred from mutant phenotype
(Pradhan-Sundd and Verheyen, 2014)
receptor internalization
inferred from mutant phenotype
(Lloyd et al., 2002)
regulation of cell cycle
inferred from genetic interaction with FLYBASE:Stam; FB:FBgn0027363
(Tognon et al., 2014)
regulation of epithelial cell migration, open tracheal system
inferred from mutant phenotype
(Chanut-Delalande et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
intracellular protein transport
inferred from electronic annotation with InterPro:IPR002014
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
endosome
inferred from direct assay
(Huang et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | faint | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval hemocyte
(Khadilkar et al., 2014)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Hrs protein is expressed in circulating hemocytes.

(Khadilkar et al., 2014)
Marker for
 
early endosome
(Vaccari et al., 2010)
Subcellular Localization
CV Term
Evidence
References
endosome
inferred from direct assay
(Huang et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hrs in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hrs
Transgenic constructs containing regulatory region of Hrs
Deletions and Duplications ( 8 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Df(2L)Exel6008
lethal, with Scer\GAL4da.G32
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage
partially lethal - majority die | adult stage, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
increased cell death | somatic clone | third instar larval stage
increased cell size | somatic clone
neuroanatomy defective, with Scer\GAL4repo.PU
neuroanatomy defective | pupal stage, with Scer\GAL4ppk.1.9
some die during larval stage | recessive
some die during pupal stage
visible, with Scer\GAL4ap-md544
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4pnr-MD237
visible | adult stage | somatic clone, with Scer\GAL4Act.PU
Phenotype manifest in
Allele
border follicle cell | somatic clone
chaeta | supernumerary, with Scer\GAL4pnr-MD237
dendrite | pupal stage, with Scer\GAL4ppk.1.9
embryonic/larval garland cell
endosome
endosome | somatic clone | third instar larval stage
extracellular exosome, with Scer\GAL4dsx.KI
eye | somatic clone
eye | somatic clone, with Scer\GAL4Act.PU
eye-antennal disc | somatic clone
eye disc, with Scer\GAL4repo.PU
eye disc | somatic clone
eye disc | somatic clone | third instar larval stage
eye photoreceptor cell, with Df(2L)Exel6277
glial cell, with Scer\GAL4repo.PU
imaginal disc epithelial cell | somatic clone
larval dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.1.9
larval dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4ppk.1.9
late endosome | somatic clone
male accessory gland secondary cell, with Scer\GAL4dsx.KI
male accessory gland secondary cell, with Scer\GAL4NP0261
male accessory gland secondary cell, with Scer\GAL4NP7397
mesothoracic tergum, with Scer\GAL4pnr-MD237
multivesicular body, internal vesicle, with Scer\GAL4NP0261
multivesicular body, with Scer\GAL4NP0261
nucleus, with Scer\GAL4NP7397
oocyte
photoreceptor | somatic clone | third instar larval stage
photoreceptor cell | somatic clone
photoreceptor cell | somatic clone | third instar larval stage
vesicle
wing, with Scer\GAL4ap-md544
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4cv-c-C5
wing disc | somatic clone | third instar larval stage
wing margin, with Scer\GAL4bbg-C96
wing margin, with Scer\GAL4cv-c-C5
wing margin bristle, with Scer\GAL4cv-c-C5
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\HGS
14 of 15
Yes
Yes
Hsap\GGA1
1 of 15
No
No
Hsap\GGA2
1 of 15
No
No
Hsap\GGA3
1 of 15
No
No
 
Hsap\TOM1L2
1 of 15
No
No
Hsap\ZFYVE1
1 of 15
No
Yes
 
Hsap\ZFYVE19
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Hgs
14 of 15
Yes
Yes
 
Mmus\Gga1
1 of 15
No
No
Mmus\Gga2
1 of 15
No
No
Mmus\Gga3
1 of 15
No
No
Mmus\Tom1l2
1 of 15
No
No
Mmus\Zfyve1
1 of 15
No
Yes
Mmus\Zfyve19
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Hgs
10 of 13
Yes
Yes
Rnor\Zfyve1
1 of 13
No
Yes
Rnor\Zfyve19
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\hgs
8 of 12
Yes
Yes
Xtro\tom1l2
1 of 12
No
No
Xtro\zfyve1
1 of 12
No
Yes
Xtro\zfyve19
1 of 12
No
No
Danio rerio (Zebrafish) (8)
Drer\hgs
14 of 15
Yes
Yes
Drer\gga1
1 of 15
No
No
Drer\si:ch211-11n16.2
1 of 15
No
Yes
Drer\slc4a1b
1 of 15
No
No
Drer\tom1l2
1 of 15
No
No
Drer\tom1l2
1 of 15
No
No
Drer\zfyve1
1 of 15
No
Yes
Drer\zfyve19
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\hgrs-1
13 of 15
Yes
Yes
Cele\apt-9
1 of 15
No
No
Cele\C07A12.7
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\AT5G16880
1 of 9
Yes
No
Atha\AT5G63640
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\VPS27
10 of 15
Yes
Yes
Scer\GGA1
1 of 15
No
No
Scer\GGA2
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\sst4
6 of 12
Yes
Yes
Spom\SPAC1F3.05
1 of 12
No
No
Spom\SPBC25H2.16c
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919064M )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Hrs
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000612
Drosophila simulans
Dsim\GD23175
Drosophila sechellia
Dsec\GM18359
Drosophila erecta
Dere\GG24878
Drosophila yakuba
Dyak\GE18175
Drosophila ananassae
Dana\GF13888
Drosophila pseudoobscura pseudoobscura
Dpse\GA15506
Drosophila persimilis
Dper\GL26577
Drosophila willistoni
Dwil\GK24576
Drosophila virilis
Dvir\GJ17377
Drosophila mojavensis
Dmoj\GI17877
Drosophila grimshawi
Dgri\GH11165
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505HQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011658
Glossina morsitans
Tsetse fly
Gmor\GMOY003119
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05821
Mayetiola destructor
Hessian fly
Mdes\Mdes011130
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005339
Anopheles darlingi
American malaria mosquito
Adar\ADAC007405
Anopheles gambiae
Malaria mosquito
Agam\AGAP008887
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001538
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05E8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007035
Danaus plexippus
Monarch butterfly
Dple\DPOGS203253-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL022504
Apis florea
Little honeybee
Aflo\2944455380
Apis mellifera
Western honey bee
Amel\Hrs
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11071
Bombus terrestris
Buff-tailed bumblebee
Bter\15208881
Linepithema humile
Argentine ant
Lhum\LH22519
Linepithema humile
Argentine ant
Lhum\LH10164
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002b6c
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008856
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12102
Tribolium castaneum
Red flour beetle
Tcas\TC015976
Pediculus humanus
Human body louse
Phum\PHUM430260
Rhodnius prolixus
Kissing bug
Rpro\RPRC001853
Cimex lectularius
Bed bug
Clec\CLEC000273
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI087535
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006279
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03881
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05AE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003447
Ixodes scapularis
Black-legged tick
Isca\ISCW002288
Stegodyphus mimosarum
African social velvet spider
Smim\KFM56719.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur09g02340
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_187221
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06JJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hgs
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hgs_1
Ciona intestinalis
Vase tunicate
Cint\zf(fyve)-11
Gallus gallus
Domestic chicken
Ggal\HGS
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG3529
1 of 10
CG41099
1 of 10
Gga
1 of 10
Wdfy2
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    HGS; hepatocyte growth factor-regulated tyrosine kinase substrate
    14 of 15
    604375
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Hrs
        suppressible
        dsh
        (Seto and Bellen, 2006)
        Hrs
        suppressible
        fz
        (Seto and Bellen, 2006)
        Hrs
        suppressible
        lilli
        (Pepple et al., 2007)
        Hrs
        enhanceable
        Rbf
        (Sheng et al., 2016)
        Hrs|sta
        suppressible
        rush
        (Gailite et al., 2012)
        Hrs
        suppressible
        rush
        (Gailite et al., 2012)
        Hrs
        enhanceable
        sta
        (Gailite et al., 2012)
        Hrs
        suppressible
        wg
        (Seto and Bellen, 2006)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        l(2)gd1
        suppressible
        Hrs
        (Schneider et al., 2013, Gallagher and Knoblich, 2006)
        Rab8
        suppressible
        Hrs
        (West et al., 2015)
        Rbf
        enhanceable
        Hrs
        (Sheng et al., 2016)
        rho
        suppressible
        Hrs
        (Tjota et al., 2011)
        sky
        suppressible
        Hrs
        (Uytterhoeven et al., 2011)
        smo
        suppressible
        Hrs
        (Fan et al., 2013)
        smo
        enhanceable
        Hrs
        (Fan et al., 2013)
        spin
        suppressible
        Hrs
        (Yuva-Aydemir et al., 2011)
        Vps35
        suppressible
        Hrs
        (Li et al., 2018)
        External Data
        Subunit Structure (UniProtKB)
        Homodimer; in vitro. Interacts with ubiquitin.
        (UniProt, Q960X8 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Negative Regulators of Torso Signaling Pathway -
        Negative regulators of Torso signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl).
        Positive Regulators of Toll-NF-KappaB Signaling Pathway -
        In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-7

        Cytogenetic map
        23A3-23A3
        Sequence location
        2L:2,739,986..2,743,377 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        23A3-23A3
        Limits computationally determined from genome sequence between P{PZ}dpp10638 and P{EP}EP832
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        23A-23A
        (Bellen, 2000.3.5, Lloyd et al., 2000)
        Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
        (Lloyd et al., 2000)
        Experimentally Determined Recombination Data
        Location

        2-7

        (FlyBase, 2016)

        2-6.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (9)
        Genomic Clones (12)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (79)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (Riedel et al., 2016)
        Commercially Available Antibodies
         
        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Hrs CG2903

        (Verstreken and Bellen, 2000.9.4)
        Source for database merge of

        Source for merge of: Hrs l(2)23AB5

        (Lloyd et al., 2002)
        Additional comments
        Other Comments

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)

        Hrs appears to regulate inward budding of endosome membrane and multivesicular body (MVB) formation.

        (Lloyd et al., 2002)

        Identification: Screen for lethals heterozygous with Df(2L)C144.

        (DiAntonio et al., 1993)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 63 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        PDB - An information portal to biological macromolecular structures
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (20)
        Reported As
        Symbol Synonym
        CG2903
        (Meadows, 2018.10.31, O'Farrell et al., 2017, Ni et al., 2010.12.1, Perkins et al., 2009, Gandhi et al., 2007, Dorer et al., 2006, Whitfield et al., 2002)
        DmHRS
        (Winter and Hauser, 2006)
        HRS
        (West et al., 2015, Nagaraj and Banerjee, 2009, Dorer et al., 2006, Jafar-Nejad et al., 2005, Langevin et al., 2005, Langevin et al., 2005, Lloyd et al., 2000)
        HRS-2
        (Littleton, 2000)
        Hrs
        (Meltzer et al., 2019, Mezzofanti et al., 2019, Xu et al., 2019, Jiang et al., 2018, Li et al., 2018, Beer and Wehman, 2017, Malartre, 2016, Riedel et al., 2016, Aradska et al., 2015, Balakrishnan et al., 2015, Legent et al., 2015, Li and Blissard, 2015, Loncle et al., 2015, Parchure et al., 2015, Pradhan-Sundd and Verheyen, 2015, Rister et al., 2015, Zang et al., 2015, Cao et al., 2014, Corrigan et al., 2014, Dodson et al., 2014, Gradilla et al., 2014, Issman-Zecharya and Schuldiner, 2014, Khadilkar et al., 2014, Parsons et al., 2014, Politi et al., 2014, Tognon et al., 2014, Valzania et al., 2014, Wu et al., 2014, Xu et al., 2014, Zhang et al., 2014, Aoyama et al., 2013, Beckett et al., 2013, Cherry et al., 2013, Fan et al., 2013, Hermle et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Mbodj et al., 2013, Muha and Müller, 2013, van den Brink et al., 2013, Yang et al., 2013, Yousefian et al., 2013, Zhang et al., 2013, Dodson et al., 2012, Gault et al., 2012, Haberman et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Troost et al., 2012, Yamakawa et al., 2012, Cho et al., 2011, Daskalaki et al., 2011, Du et al., 2011, Halbsgut et al., 2011, Kulkarni et al., 2011, Lee et al., 2011, Leventis et al., 2011, Liu et al., 2011, Mukherjee et al., 2011, Otani et al., 2011, Powis and Macdougall, 2011, Rodal et al., 2011, Tjota et al., 2011, Yamada et al., 2011, Chanut-Delalande et al., 2010, Haberman et al., 2010, Ho et al., 2010, Huang et al., 2010, Kim et al., 2010, Li et al., 2010, Lone et al., 2010, Michelet et al., 2010, Mottola et al., 2010, Padash-Barmchi et al., 2010, Richardson and Pichaud, 2010, Vaccari et al., 2010, Vidal et al., 2010, Williamson et al., 2010, Windler and Bilder, 2010, Akbar et al., 2009, Coumailleau et al., 2009, Gilbert et al., 2009, Gilbert et al., 2009, Herz et al., 2009, Rodahl et al., 2009, Harris and Tepass, 2008, Juhász et al., 2008, Miura et al., 2008, Vaccari et al., 2008, Yamada et al., 2008, Bianco et al., 2007, Blankenship et al., 2007, Chanut-Delalande et al., 2007, Childress et al., 2007, Commisso and Boulianne, 2007, Rusten et al., 2007, Sasamura et al., 2007, Bokel et al., 2006, Childress et al., 2006, Childress et al., 2006, Giebel and Wodarz, 2006, Hennig et al., 2006, Rusten et al., 2006, Shimada et al., 2006, Emery et al., 2005, Jekely et al., 2005, Rodrigues et al., 2005, Wang et al., 2004)
        Vps27
        (Kim et al., 2010)
        dHrs
        (Huang et al., 2010)
        hrs
        (O'Farrell et al., 2017, Sheng et al., 2016, Chang et al., 2013, Fan et al., 2013, Schneider et al., 2013, Gailite et al., 2012, Li et al., 2012, Kopyl et al., 2011, Uytterhoeven et al., 2011, Yuva-Aydemir et al., 2011, Chanut-Delalande et al., 2010, Lee et al., 2009, Vaccari et al., 2009, Yan et al., 2009, Belenkaya et al., 2008, Haberman et al., 2008, Strutt and Strutt, 2008, Devergne et al., 2007, Irion and St Johnston, 2007, Irion and St Johnston, 2007, Pepple et al., 2007, Gallagher and Knoblich, 2006, Giebel and Wodarz, 2006, Jaekel and Klein, 2006, Rives et al., 2006, Seto and Bellen, 2006, Lu and Bilder, 2005, Vaccari and Bilder, 2005, Bache et al., 2004, Seto and Bellen, 2004, Wang and Struhl, 2004, Jekely and Rorth, 2003, Lloyd et al., 2000)
        l(2)23AB5
        (DiAntonio et al., 1993)
        l(2)23Ad
        (Lloyd et al., 2002, Hartenstein et al., 1997, Littleton and Bellen, 1994)
        l(2)23Ad(Hrs)
        (Wang et al., 2004)
        vps27
        (Ahmad et al., 2009, Irion and St Johnston, 2007)
        Name Synonyms
        F1F0 ATPase
        (Otani et al., 2011)
        HGF-regulated tyrosine kinase substrate
        (Fan et al., 2013)
        Hepatocyte growth factor regulated tyrosine kinase
        (Rodrigues et al., 2005)
        Hepatocyte growth factor regulated tyrosine kinase substrate
        (Malartre, 2016, Beckett et al., 2013, Du et al., 2011, Chang et al., 2008, Mao et al., 2000)
        Hepatocyte growth factor-regulated tyrosine kinase substrate
        (Daskalaki et al., 2011, Chanut-Delalande et al., 2010, Padash-Barmchi et al., 2010)
        Hepatocyte growth factor-regulated tyrosine substrate
        (Valzania et al., 2014)
        Hepatocyte growth-factor-regulated substrate
        (Childress et al., 2006)
        hepatocyte growth factor-regulated tyrosine kinase substrate
        (Liu et al., 2011, Belenkaya et al., 2008, Seto and Bellen, 2006)
        Secondary FlyBase IDs
        • FBgn0013527
        • FBgn0041149
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (216)