FB2020_06, released Dec 22, 2020

Gene: Dmel\Spindly

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General Information
Symbol
Dmel\Spindly
Species
D. melanogaster
Name
Spindly
Annotation Symbol
CG15415
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031549
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
Spindly (Spindly) encodes a key regulator of dynein recruitment to kinetochores and is involved in the spindle assembly checkpoint during the cell cycle. It also contributes to cell morphology and migration. [Date last reviewed: 2018-11-15]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
24A1-24A1
Sequence location
2L:3,527,387..3,530,317 [-]
Recombination map

2-10

RefSeq locus
NT_033779 REGION:3527387..3530317
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein phosphatase 1 binding
inferred from physical interaction with FLYBASE:flw; FB:FBgn0000711
(Bennett et al., 2006)
inferred from physical interaction with FLYBASE:Pp1-13C; FB:FBgn0003132
(Bennett et al., 2006)
inferred from physical interaction with FLYBASE:Pp1α-96A; FB:FBgn0003134
(Bennett et al., 2006)
inferred from physical interaction with FLYBASE:Pp1-87B; FB:FBgn0004103
(Bennett et al., 2006)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of mitotic metaphase/anaphase transition
inferred from mutant phenotype
(Griffis et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in kinetochore
inferred from direct assay
(Griffis et al., 2007)
located_in microtubule
inferred from direct assay
(Griffis et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Summary (Interactive Fly)
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\Spindly for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.46

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Spindly-RB
    FBtr0301590
    NM_134947
    2543
    781
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Spindly-PB
    FBpp0290805
    88.6
    781
    6.13
    NP_608791
    AAF51090
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Spindly using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in kinetochore
    inferred from direct assay
    (Griffis et al., 2007)
    located_in microtubule
    inferred from direct assay
    (Griffis et al., 2007)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Spindly in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 1 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 9 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Spindly
    Transgenic constructs containing regulatory region of Spindly
    Deletions and Duplications ( 1 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Hsap\SPDL1
    2 of 15
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Mmus\Spdl1
    2 of 15
    Yes
    Yes
    Rattus norvegicus (Norway rat) (1)
    Rnor\Spdl1
    1 of 13
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (1)
    Xtro\spdl1
    2 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (1)
    Drer\spdl1
    1 of 15
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906EF )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00000905
    Drosophila simulans
    Dsim\GD22747
    Drosophila sechellia
    Dsec\GM18139
    Drosophila erecta
    Dere\GG24428
    Drosophila yakuba
    Dyak\GE14842
    Drosophila ananassae
    Dana\GF14665
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA13712
    Drosophila persimilis
    Dper\GL19471
    Drosophila willistoni
    Dwil\GK14670
    Drosophila virilis
    Dvir\GJ17596
    Drosophila mojavensis
    Dmoj\GI17772
    Drosophila grimshawi
    Dgri\GH13193
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091507RU )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17022888
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY007031
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_09658
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes001781
    Aedes aegypti
    Yellow fever mosquito
    Aaeg\AAEL002921
    Anopheles gambiae
    Malaria mosquito
    Agam\AGAP013500
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ003891
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Spindly - Ald1
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG12768
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG16959
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG2064
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG31729
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG6550
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - CG8654
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Cnx99A
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Echs1
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - FipoQ
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Idh3a
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Inos
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - MRP
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Mes2
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Nfs1
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Ost48
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - OstΔ
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Pp1-13C
      two hybrid
      (Bennett et al., 2006)
      Spindly - Pp1-87B
      two hybrid
      (Bennett et al., 2006)
      Spindly - Pp1α-96A
      two hybrid
      (Bennett et al., 2006)
      Spindly - Pp2A-29B
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - RpS10b
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - RpS5a
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Rpn11
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - SERCA
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Sc2
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Sec61α
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Sec63
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Sil1
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - UQCR-C2
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - ZnT63C
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - Zwilch
      pull down, anti tag western blot
      (Barbosa et al., 2020)
      Spindly - fax
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - flw
      two hybrid
      (Bennett et al., 2006)
      Spindly - lic
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - nsl1
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Raja et al., 2010)
      Spindly - p23
      experimental knowledge based
      (Guruharsha et al., 2011)
      Spindly - polo
      enzymatic study, autoradiography
      (Barbosa et al., 2020)
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map

      2-10

      Cytogenetic map
      24A1-24A1
      Sequence location
      2L:3,527,387..3,530,317 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      24A1-24A1
      Limits computationally determined from genome sequence between P{EP}ThorEP818&P{lacW}Thork07736 and P{PZ}oddrF111&P{PZ}for06860
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      2-10

      (FlyBase, 2016)

      2-8.7

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (4)
      Genomic Clones (20)
      cDNA Clones (8)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Barbosa et al., 2020)
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: Spindly CG15415

          (Griffis et al., 2007)
          Source for database merge of
          Additional comments
          Other Comments

          The Spindly product recruits dynein to kinetochores.

          (Griffis et al., 2007)

          dsRNA has been synthesised for this gene and transfected into S2 cells. Spindly dsRNA causes interphase S2 cells to adopt an abnormal morphology with spiky and elongated microtubule-rich projections. The majority of dsRNA-treated cells become arrested in metaphase, an effect which can be rescued with a Spindly transgene. Spindly-depleted cells require 50% more time to form a metaphase plate compared with untreated cells.

          (Griffis et al., 2007)

          RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

          (Boutros et al., 2004)
          Origin and Etymology
          Discoverer
          Etymology

          Named "Spindly" due to the spindle-shaped interphase cell morphology and metaphase spindle phenotypes observed when this gene product is depleted.

          (Griffis et al., 2007)
          Identification
          External Crossreferences and Linkouts ( 21 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (3)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (33)