• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\CG3294

General Information
Symbol	Dmel\CG3294	Species	D. melanogaster
Name		Annotation symbol	CG3294
Feature type	protein_coding_gene	FlyBase ID	FBgn0031628
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	25A6-25A6	Sequence location	2L:4,794,549..4,796,576 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG3294 (FBgn0031628). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: mRNA binding. Based on sequence similarity, it is predicted to be involved in the biological process: nuclear mRNA splicing, via spliceosome. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain; U2 auxiliary factor small subunit; Zinc finger, CCCH-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1631181_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG3294. Gene sequence location is 2L:4794549..4796576.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN24879 HFA00590
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25A6-25A6   Limits computationally determined from genome sequence between P{lacW}Tps1k08903&P{EP}morgueEP1184 and P{lacW}l(2)k10004k10004&P{EP}CG3036EP963
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG3294 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG3294-RA FBtr0077381  NM_135013   1507   456 CG3294-RB FBtr0077382  NM_175962   1506   314
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG3294-PA   FBpp0077073   54.8   456   9.76     NP_608857   AAF50982 CG3294-PB   FBpp0077074   38.3   314   9.35     NP_787976   AAO41150
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Zinc finger, CCCH-type (IPR000571) U2 auxiliary factor small subunit (IPR009145) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA736052   AA821135   AE014134 AAF50982   AE014134 AAO41150   AI108542   AI257160   AI519018   AY122140 AAM52652   BF504993   BG636098   BI632442   CO326123   CZ484575   EC211181   EC251799
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q86BN2 Q8MR40 Q9VR26
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0031628    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0031628 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0031628 expression level Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (196) embryo 02-04hr  (609) embryo 04-06hr  (609) embryo 06-08hr  (449) embryo 08-10hr  (404) embryo 10-12hr  (331) embryo 12-14hr  (300) embryo 14-16hr  (242) embryo 16-18hr  (191) embryo 18-20hr  (142) embryo 20-22hr    76 embryo 22-24hr  105 larva L1  (190) larva L2  (182) larva L3 12hr old  (139) larva L3 puffstage 1-2  (214) larva L3 puffstage 3-6  (320) larva L3 puffstage 7-9  (300) white prepupae new  (319) white prepupae 12hr  (416) white prepupae 24hr  (535) pupae 2d postWPP  (417) pupae 3d postWPP  (283) pupae 4d postWPP  (274) adult male 01day  (396) adult male 05day  (538) adult male 30day  (561) adult female 01day  (246) adult female 05day  (288) adult female 30day  (308) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0031628 expression level Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (196) embryo 02-04hr  (609) embryo 04-06hr  (609) embryo 06-08hr  (449) embryo 08-10hr  (404) embryo 10-12hr  (331) embryo 12-14hr  (300) embryo 14-16hr  (242) embryo 16-18hr  (191) embryo 18-20hr  142 embryo 20-22hr    76 embryo 22-24hr  105 larva L1  (190) larva L2  (182) larva L3 12hr old  139 larva L3 puffstage 1-2  (214) larva L3 puffstage 3-6  (320) larva L3 puffstage 7-9  (300) white prepupae new  (319) white prepupae 12hr  (416) white prepupae 24hr  (535) pupae 2d postWPP  (417) pupae 3d postWPP  (283) pupae 4d postWPP  (274) adult male 01day  (396) adult male 05day  (538) adult male 30day  (561) adult female 01day  (246) adult female 05day  (288) adult female 30day  (308) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    196 embryo 02-04hr    609 embryo 04-06hr    609 embryo 06-08hr    449 embryo 08-10hr    404 embryo 10-12hr    331 embryo 12-14hr    300 embryo 14-16hr    242 embryo 16-18hr    191 embryo 18-20hr    142 embryo 20-22hr    76 embryo 22-24hr    105 larva L1    190 larva L2    182 larva L3 12hr old    139 larva L3 puffstage 1-2    214 larva L3 puffstage 3-6    320 larva L3 puffstage 7-9    300 white prepupae new    319 white prepupae 12hr    416 white prepupae 24hr    535 pupae 2d postWPP    417 pupae 3d postWPP    283 pupae 4d postWPP    274 adult male 01day    396 adult male 05day    538 adult male 30day    561 adult female 01day    246 adult female 05day    288 adult female 30day    308 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0031628    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0031628 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0031628 expression level Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0031628 expression level Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    61.9 Larval Midgut no informative data Larval Hindgut    19.1 Larval Malpighian Tubules    19.2 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    28.575 Larval Carcass no informative data Adult Head    25.5 Adult Eye    41.375 Adult Brain    56.5 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21 Adult Midgut    23.3 Adult Hindgut    24.7 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    14.675 Adult VirginFemale Spermatheca    26.9 Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    21.3 Adult Testis    16.3 Adult Male Accessory Gland    28.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    25.5   NA  Eye    41.375   NA  Brain    56.5   61.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    45   NA  Crop    21   no informative data  Midgut    23.3   19.1  Hindgut    24.7   19.2  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    14.675   28.575  Trachea    NA   NA  VirginFemale Spermatheca    26.9   NA  InseminatedFemale Spermatheca    21.9   NA  Ovary    21.3   NA  Testis    16.3   NA  Male Accessory Gland    28.2   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031628 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3294-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG3294f02075 viable, with Scer\GAL4pnr-MD237 CG3294GD10784 Sterility Allele fertile CG3294f02075 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG3294 Class Mutagen Stocks Known lesion CG3294f02075 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG3294 Class Mutagen Stocks Known lesion CG3294GD10784 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG3294JF02606 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10784} NA P{TRiP.JF02606} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}CG3294f02075 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-) RNA binding inferred from electronic annotation with InterPro:IPR009145 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR000571 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
nuclear mRNA splicing, via spliceosome inferred from sequence or structural similarity with NCBI:BAA08533 (Mount and Salz, 2000)
Cellular Component
CV term Evidence References
spliceosomal complex inferred from sequence or structural similarity with NCBI:BAA08533 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG3294 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59866 DroID - A comprehensive database of gene and protein interactions. • FBgn0031628 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33677
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21431 Dere\GG25018 Dgri\GH11518 Dmoj\GI17015 Dper\GL14474 Dpse\GA17206 Dsec\GM18489 Dsim\GD23299 Dvir\GJ24411 Dwil\GK23757 Dyak\GE18306
Curated drosophilid orthologs	Dpse\GA17206
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL011071-PA Aedes aegypti (mosquito) AGAP010194-PA Anopheles gambiae (mosquito) XP_392139.1 Apis mellifera (honey bee) At1g10320.1 Arabidopsis thaliana (wall cress) 123654 Branchiostoma floridae (lancelet) ENSBTAP00000027211 Bos taurus (bovine) ENSCAFP00000033520 Canis familiaris (dog) CPIJ005985 Culex pipens (mosquito) ENSCPOP00000003813 Cavia porcellus (guinea pig) ENSDARP00000075762 Danio rerio (zebra fish) ENSECAP00000004944 Equus caballus (horse) ENSGACP00000019877 Gymnopilus aculeatus (mushroom) ENSGALP00000026660 Gallus gallus (chicken) ENSP00000303015 Homo sapiens (human) ISCW019452-PA Ixodes scapularis (deer tick) 235470 Lottia gigantea (owl limpet) ENSMODP00000021460 Monodelphis domestica (gray opossum) ENSMMUP00000018472 Macaca mulatta (rhesus monkey) ENSMUSP00000107908 Mus musculus (house mouse) XP_001608048.1 Nasonia vitripennis (wasp) ENSOANP00000004075 Ornithorynchus anatinus (platypus) ENSORLP00000004323 Oryzias latipes (japanese killifish) LOC_Os02g35150.1 Oryza sativa (asian rice) PHUM007125-PA Pediculus humanus (human body louse) ENSPPYP00000022540 Pongo pygmaeus (orangutan) ENSPTRP00000044230 Pan troglodytes (chimpanzee) ENSRNOP00000012768 Rattus norvegicus (norway rat) XP_001185589.1 Strongylocentrotus purpuratus (purple sea urchin) 55208 Trichoplax adhaerens (metazoa) XP_973215.2 Tribolium castaneum (red flour beetle) ENSTNIP00000012368 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000043777 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18692 NV10444 LH18520 FBgn0235731 FBgn0225719 FBgn0211491 FBgn0194685 FBgn0173396 FBgn0077220 FBgn0152079 FBgn0139760 FBgn0118998 FBgn0117147 FBgn0098433 CPIJ005985 BGIBMGA006197 GB11464 AGAP010194 AAEL015434 AAEL011071 PHUM151280 GLEAN_10403
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	18512 w1118; PBac{WH}CG3294f02075 27296 y1 v1; P{TRiP.JF02606}attP2
Harvard	- PBac{WH}CG3294f02075
VDRC	v26110 w1118; P{GD10784}v26110/TM3 v26111 w1118; P{GD10784}v26111/TM3
Genomic Clones ( 1 )
	BACR04C19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM14666
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH07726 GM10887 LP05448 SD13690 SD26223
Other clones	AT07148 EP03258
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0031628 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33677
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135013 NM_175962 RefSeq (Proteins) • NP_608857 NP_787976 DDBJ / EMBL / GenBank • AA821135 AAF50982 AAM52652 AAO41150 AI519018 AY122140 CZ484575 Entrez Gene - A searchable database of RefSeq genes. • 33677 UniProtKB/TrEMBL • Q8MR40 Q9VR26 Q86BN2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Zinc finger, CCCH-type (IPR000571) U2 auxiliary factor small subunit (IPR009145) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59866 DroID - A comprehensive database of gene and protein interactions. • FBgn0031628 DRSC - Results from RNAi screens. • FBgn0031628 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031628 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3294-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031628 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33677 InParanoid A subset of ortholog calls from InParanoid. • AAEL011071-PA Aedes aegypti (mosquito) AGAP010194-PA Anopheles gambiae (mosquito) XP_392139.1 Apis mellifera (honey bee) At1g10320.1 Arabidopsis thaliana (wall cress) 123654 Branchiostoma floridae (lancelet) ENSBTAP00000027211 Bos taurus (bovine) ENSCAFP00000033520 Canis familiaris (dog) CPIJ005985 Culex pipens (mosquito) ENSCPOP00000003813 Cavia porcellus (guinea pig) ENSDARP00000075762 Danio rerio (zebra fish) ENSECAP00000004944 Equus caballus (horse) ENSGACP00000019877 Gymnopilus aculeatus (mushroom) ENSGALP00000026660 Gallus gallus (chicken) ENSP00000303015 Homo sapiens (human) ISCW019452-PA Ixodes scapularis (deer tick) 235470 Lottia gigantea (owl limpet) ENSMODP00000021460 Monodelphis domestica (gray opossum) ENSMMUP00000018472 Macaca mulatta (rhesus monkey) ENSMUSP00000107908 Mus musculus (house mouse) XP_001608048.1 Nasonia vitripennis (wasp) ENSOANP00000004075 Ornithorynchus anatinus (platypus) ENSORLP00000004323 Oryzias latipes (japanese killifish) LOC_Os02g35150.1 Oryza sativa (asian rice) PHUM007125-PA Pediculus humanus (human body louse) ENSPPYP00000022540 Pongo pygmaeus (orangutan) ENSPTRP00000044230 Pan troglodytes (chimpanzee) ENSRNOP00000012768 Rattus norvegicus (norway rat) XP_001185589.1 Strongylocentrotus purpuratus (purple sea urchin) 55208 Trichoplax adhaerens (metazoa) XP_973215.2 Tribolium castaneum (red flour beetle) ENSTNIP00000012368 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000043777 Takifugu rubripes (pufferfish Takifugu) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33677 modMine - Data generated by the modENCODE project. • FBgn0031628 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18692 NV10444 LH18520 FBgn0235731 FBgn0225719 FBgn0211491 FBgn0194685 FBgn0173396 FBgn0077220 FBgn0152079 FBgn0139760 FBgn0118998 FBgn0117147 FBgn0098433 CPIJ005985 BGIBMGA006197 GB11464 AGAP010194 AAEL015434 AAEL011071 PHUM151280 GLEAN_10403
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	cg3294 (Lasko, 2000) CG3294 (Perkins et al., 2009.8.10, Campos et al., 2010, Pessa et al., 2010)
Name Synonym
Secondary FlyBase IDs
References ( 20 )
Research paper	Campos et al., 2010, Genetics 184(1): 129--140 Genetic screen in Drosophila melanogaster uncovers a novel set of genes required for embryonic epithelial repair. [FBrf0209682] Pessa et al., 2010, PLoS ONE 5(10): e13215 Gene expression profiling of U12-type spliceosome mutant Drosophila reveals widespread changes in metabolic pathways. [FBrf0212091] Schneider et al., 2004, Proc. Natl. Acad. Sci. USA 101(26): 9584--9589 Identification of an evolutionarily divergent U11 small nuclear ribonucleoprotein particle in Drosophila. [FBrf0179434] Lasko, 2000, J. Cell Biol. 150(2): F51--F56 The Drosophila melanogaster genome: translation factors and RNA binding proteins. [FBrf0129923] Mount and Salz, 2000, J. Cell Biol. 150(2): F37--F43 Pre-messenger RNA processing factors in the Drosophila genome. [FBrf0129982]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Perkins et al., 2009.8.10, Update to TRiP stock collection. Update to TRiP stock collection. [FBrf0208863] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Robertson, 2001.7.7, Third set of annotation suggestions from bee ESTs. Third set of annotation suggestions from bee ESTs. [FBrf0138600]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
Curated genome annotation	Lasko, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126679]