FlyBase Gene Report: Dmel\Marcal1

General Information

Symbol

Dmel\Marcal1

Species

D. melanogaster

Name

Marcal1

Annotation Symbol

CG3753

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031655

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Snapshot

Marcal1 (Marcal1) encodes an ATP-dependent DNA annealing helicase that recognizes single-stranded to double-stranded DNA transitions. It maintains genomic integrity through its roles in DNA repair, DNA replication, DNA recombination and gene expression. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

25B4-25B4

Sequence location

2L:4,955,509..4,958,292 [+]

Recombination map

2-14

RefSeq locus

NT_033779 REGION:4955509..4958292

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables annealing helicase activity

inferred from direct assay

(Kassavetis and Kadonaga, 2014)

enables four-way junction helicase activity

inferred from direct assay

(Kassavetis and Kadonaga, 2014)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables annealing helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:Q9NZC9

(assigned by UniProt )

(UniProt Curators, 2002-)

enables ATP binding

inferred from electronic annotation with InterPro:IPR000330

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables DNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

enables DNA-dependent ATPase activity

inferred from sequence or structural similarity with UniProtKB:Q9NZC9

(assigned by UniProt )

(UniProt Curators, 2002-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in DNA rewinding

inferred from direct assay

(Kassavetis and Kadonaga, 2014)

involved_in DNA strand renaturation involved in double-strand break repair

inferred from mutant phenotype

(Holsclaw and Sekelsky, 2017)

involved_in heteroduplex formation involved in double-strand break repair via synthesis-dependent strand annealing

inferred from mutant phenotype

(Holsclaw and Sekelsky, 2017)

NOT involved_in replication fork reversal

inferred from direct assay

(Kassavetis and Kadonaga, 2014)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in DNA metabolic process

inferred from sequence or structural similarity with UniProtKB:Q9NZC9

(assigned by UniProt )

(UniProt Curators, 2002-)

involved_in DNA repair

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in DNA rewinding

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of transcription by RNA polymerase II

inferred from sequence or structural similarity with UniProtKB:Q9NZC9

(assigned by UniProt )

(UniProt Curators, 2002-)

involved_in replication fork processing

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in replication fork protection

inferred from biological aspect of ancestor with PANTHER:PTN002908525

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Baradaran-Heravi et al., 2012)

located_in polytene chromosome interband

inferred from direct assay

(Baradaran-Heravi et al., 2012)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

colocalizes_with DNA replication factor A complex

inferred from biological aspect of ancestor with PANTHER:PTN000084485

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nuclear replication fork

inferred from biological aspect of ancestor with PANTHER:PTN002244927

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from sequence or structural similarity with UniProtKB:Q9NZC9

(assigned by UniProt )

(UniProt Curators, 2002-)

Gene Group (FlyBase)

ANNEALING HELICASES

Protein Family (UniProt)

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily. (Q9VMX6)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ANNEALING HELICASES -: Annealing helicases catalyze the ATP-dependent rewinding of single-stranded DNA to reform base pairs between strands (therefore catalyzing the reverse reaction to that of DNA helicases). The best characterized annealing helicase is SMARCAL1 (also known as HARP), which has roles in DNA repair, telomere maintenance and replication fork stability in response to DNA replication stress. (Adapted from PMID:28623093.)

Protein Function (UniProtKB)

ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA.

(UniProt, Q9VMX6)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Marcal1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Marcal1-RA

FBtr0078992

NM_135039

2411

755

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Marcal1-PA

FBpp0078631

84.6

755

9.38

NP_608883

AAF52182

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VMX6

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Marcal1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Marcal1_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Baradaran-Heravi et al., 2012)

located_in polytene chromosome interband

inferred from direct assay

(Baradaran-Heravi et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Marcal1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Marcal1

View exonic expression by tissue for Dmel\Marcal1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0031655

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3753-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3753

Flygut - An atlas of the Drosophila adult midgut

FBgn0031655

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Marcal1

Transgenic constructs containing regulatory region of Marcal1

Deletions and Duplications ( 2 )

Disrupted in

Df(2L)Exel7021

(Ryder, 2004.4.26)

Df(2L)Exel8012

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Marcal1^GD11065

viable, with Scer\GAL4^Mef2.PR

Marcal1^GD11065

viable, with Scer\GAL4^pnr-MD237

Marcal1^GD11065

viable, with Scer\GAL4^tin.CΔ4

Marcal1^GD11065

Other Phenotypes

Allele

DNA repair defective | adult stage

Marcal1^K275M

DNA repair defective | adult stage (with Marcal1^del)

Marcal1^kh1

DNA repair defective | adult stage (with Marcal1^kh1)

Marcal1^del

short lived | heat sensitive

Marcal1^del

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Marcal1^GD11065

visible, with Scer\GAL4^pnr-MD237

Marcal1^GD11065

visible, with Scer\GAL4^Tub.PU

visible | adult stage, with Scer\GAL4^Tub.PU

Marcal1^UAS.cBa

Phenotype manifest in

Allele

adult pericardial cell, with Scer\GAL4^Ugt36A1.PK

Marcal1^HMS00587

egg | heat sensitive

Marcal1^del

trichogen cell, with Scer\GAL4^pnr-MD237

Marcal1^GD11065

wing vein | adult stage, with Scer\GAL4^Tub.PU

Marcal1^UAS.cBa

wing vein | ectopic, with Scer\GAL4^Tub.PU

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SMARCAL1

13 of 15

Yes

Hsap\ZRANB3

4 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Smarcal1

13 of 15

Yes

Mmus\Zranb3

4 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Smarcal1

11 of 13

Yes

Rnor\Zranb3

3 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\smarcal1

11 of 12

Yes

Xtro\zranb3

2 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\smarcal1

11 of 15

Yes

Drer\zranb3

2 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\smrc-1

15 of 15

Yes

Cele\csb-1

1 of 15

Yes

Cele\M03C11.8

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\CHR18

8 of 9

Yes

Atha\AT5G07810

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905NE )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17662

Drosophila persimilis

Dper\GL26533

Drosophila willistoni

Dwil\GK24475

Drosophila virilis

Dvir\GJ16178

Drosophila mojavensis

Dmoj\GI17136

Drosophila grimshawi

Dgri\GH11691

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915025L )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17004455

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_06803

Mayetiola destructor

Hessian fly

Mdes\Mdes007717

Mayetiola destructor

Hessian fly

Mdes\Mdes002908

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006963

Anopheles darlingi

American malaria mosquito

Adar\ADAC007599

Anopheles gambiae

Malaria mosquito

Agam\AGAP008426

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015879

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06O2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009112

Danaus plexippus

Monarch butterfly

Dple\DPOGS201030-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002379

Apis florea

Little honeybee

Aflo\2381457732

Apis mellifera

Western honey bee

Amel\LOC551616

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13138

Bombus terrestris

Buff-tailed bumblebee

Bter\15207657

Linepithema humile

Argentine ant

Lhum\LH13530

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002121

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG035818

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003767

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12143

Tribolium castaneum

Red flour beetle

Tcas\TC005426

Pediculus humanus

Human body louse

Phum\PHUM440690

Rhodnius prolixus

Kissing bug

Rpro\RPRC005085

Cimex lectularius

Bed bug

Clec\CLEC004718

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000862

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04550

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06JK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003716

Strigamia maritima

European centipede

Smar\SMAR011664

Ixodes scapularis

Black-legged tick

Isca\ISCW023702

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63101.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g01600

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_232046

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0A1B )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Smarcal1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008557gn

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009308

Gallus gallus

Domestic chicken

Ggal\ZRANB3

Gallus gallus

Domestic chicken

Ggal\SMARCAL1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (16)

Gene Symbol

Score

Source

Alignment

2 of 10

brm

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

dom

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

kis

1 of 10

lds

1 of 10

Mi-2

1 of 10

okr

1 of 10

XNP

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Schimke immuno-osseous dysplasia

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Marcal1^UAS.cBa

model of Schimke immuno-osseous dysplasia

CEA

(Morimoto et al., 2016)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SMARCAL1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1

model of Schimke immuno-osseous dysplasia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Marcal1^UAS.cBa

model of Schimke immuno-osseous dysplasia

is ameliorated by HDAC1³⁰³

(Morimoto et al., 2016)

is ameliorated by HDAC1³²⁸

(Morimoto et al., 2016)

is ameliorated by fz^EY13696

(Morimoto et al., 2016)

is ameliorated by HDAC1^def24

(Morimoto et al., 2016)

is ameliorated by gro¹

(Morimoto et al., 2016)

is ameliorated by mam²

(Morimoto et al., 2016)

is ameliorated by CtBP^87De-10

(Morimoto et al., 2016)

is ameliorated by N^nd-1

(Morimoto et al., 2016)

is ameliorated by H²

(Morimoto et al., 2016)

is ameliorated by mam⁸

(Morimoto et al., 2016)

is ameliorated by H³

(Morimoto et al., 2016)

is ameliorated by N^nd-3

(Morimoto et al., 2016)

is ameliorated by nej^Q7

(Morimoto et al., 2016)

is exacerbated by sgg¹

(Morimoto et al., 2016)

is ameliorated by Psn¹⁴³

(Morimoto et al., 2016)

is ameliorated by wg¹

(Morimoto et al., 2016)

is ameliorated by Psn⁹

(Morimoto et al., 2016)

is ameliorated by wg^l-12

(Morimoto et al., 2016)

is ameliorated by Ser¹

(Morimoto et al., 2016)

is ameliorated by Su(H)¹

(Morimoto et al., 2016)

is ameliorated by Su(H)²

(Morimoto et al., 2016)

is ameliorated by Su(H)^IB115

(Morimoto et al., 2016)

is ameliorated by arm¹

(Morimoto et al., 2016)

is ameliorated by arm²

(Morimoto et al., 2016)

is ameliorated by arm⁴

(Morimoto et al., 2016)

is ameliorated by arm⁸

(Morimoto et al., 2016)

is ameliorated by arm^G0192

(Morimoto et al., 2016)

is ameliorated by dsh¹

(Morimoto et al., 2016)

is ameliorated by dsh³

(Morimoto et al., 2016)

is ameliorated by dsh⁶

(Morimoto et al., 2016)

is ameliorated by fng¹³

(Morimoto et al., 2016)

is ameliorated by fng⁵²

(Morimoto et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SMARCAL1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1

13 of 15

606622

SCHIMKE IMMUNOOSSEOUS DYSPLASIA; SIOD

Schimke immuno-osseous dysplasia

ZRANB3; zinc finger RANBP2-type containing 3

4 of 15

615655

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Marcal1

suppressible

ash1

(Morimoto et al., 2016)

Marcal1

enhanceable

Asx

(Morimoto et al., 2016)

Marcal1

suppressible

Asx

(Morimoto et al., 2016)

Marcal1

suppressible

brm

(Morimoto et al., 2016)

Marcal1

enhanceable

crm

(Morimoto et al., 2016)

Marcal1

suppressible

E(Pc)

(Morimoto et al., 2016)

Marcal1

suppressible

E(z)

(Morimoto et al., 2016)

Marcal1

suppressible

Iswi

(Morimoto et al., 2016)

Marcal1

enhanceable

kis

(Morimoto et al., 2016)

Marcal1

suppressible

kis

(Morimoto et al., 2016)

Marcal1

enhanceable

mor

(Morimoto et al., 2016)

Marcal1

enhanceable

osa

(Morimoto et al., 2016)

Marcal1

suppressible

osa

(Morimoto et al., 2016)

Marcal1

enhanceable

(Morimoto et al., 2016)

Marcal1

enhanceable

Pcl

(Morimoto et al., 2016)

Marcal1

suppressible

Pcl

(Morimoto et al., 2016)

Marcal1

enhanceable

ph-d

(Morimoto et al., 2016)

Marcal1

suppressible

ph-p

(Morimoto et al., 2016)

Marcal1

suppressible

Psc

(Morimoto et al., 2016)

Marcal1

suppressible

psq

(Morimoto et al., 2016)

Marcal1

enhanceable

Sce

(Morimoto et al., 2016)

Marcal1

suppressible

Snr1

(Morimoto et al., 2016)

Marcal1

suppressible

Su(z)2

(Morimoto et al., 2016)

Marcal1

suppressible

(Morimoto et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brm

enhanceable

Marcal1

(Morimoto et al., 2016)

dom

suppressible

Marcal1

(Ellis et al., 2015)

suppressible

Marcal1

(Morimoto et al., 2016)

Pc|ph-p

suppressible

Marcal1

(Morimoto et al., 2016)

ph-p

suppressible

Marcal1

(Morimoto et al., 2016)

Psc

suppressible

Marcal1

(Morimoto et al., 2016)

Scm

suppressible

Marcal1

(Morimoto et al., 2016)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031655

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33709

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-14

Cytogenetic map

25B4-25B4

Sequence location

2L:4,955,509..4,958,292 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25B4-25B4

Limits computationally determined from genome sequence between P{lacW}l(2)k10004^k10004&P{EP}CG3036^EP963 and P{PZ}l(2)05714⁰⁵⁷¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-14

(FlyBase, 2016)

2-16.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Bloomington

77101

y¹ sc^* v¹ sev²¹; P{TKO.GS00896}attP40

80729

y¹ sc^* v¹ sev²¹; P{TOE.GS01519}attP40

33709

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00587}attP2

16492

y¹; P{SUPor-P}KG09850a P{SUPor-P}KG09850b Marcal1^KG09850/CyO

VDRC

v34701

w¹¹¹⁸; P{GD11065}v34701

v34702

w¹¹¹⁸; P{GD11065}v34702/CyO

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (178)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE44811

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031655

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031655

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33709

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Morimoto et al., 2016, Baradaran-Heravi et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Mutants show reduced egg laying and hatching implying that Marcal1 acts as a maternal component. In addition, mutants have an abnormal eye structure and temperature-sensitive sterility and lethality.

(Cho et al., 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33709

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VMX6

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031655

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Marcal1_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3753

Flygut - An atlas of the Drosophila adult midgut

FBgn0031655

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33709

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005426

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3753

modMine - A data warehouse for the modENCODE project

FBgn0031655

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031655

DRSC - Results frm RNAi screens

FBgn0031655

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0031655

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3753-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031655

FlyMine - An integrated database for Drosophila genomics

FBgn0031655

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33709

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG3753

(Hosono et al., 2015, Ni et al., 2010.12.1, Schneiderman et al., 2009, Jafar-Nejad et al., 2006, Cho et al., 2004, Mount, 2001.8.14)

HARP

(Kassavetis and Kadonaga, 2014)

MARCAL1

(Ellis et al., 2015)

Marca1

(Schneiderman et al., 2009)

Marcal1

(Fu et al., 2017, Hermle et al., 2017, Holsclaw and Sekelsky, 2017, Transgenic RNAi Project members, 2017-, Morimoto et al., 2016, Morimoto et al., 2016, Kim et al., 2015, Morimoto and Boerkoel, 2014.3.07, Baradaran-Heravi et al., 2012)

dmHARP

(Kassavetis and Kadonaga, 2014)

Name Synonyms

Marcal1

dsmarcal1

(Cho et al., 2004)

hepA-related protein

(Kassavetis and Kadonaga, 2014)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (39)

The following external sites may use different assemblies or annotations than FlyBase.

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