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Gene Dmel\Bub1

General Information
Symbol	Dmel\Bub1	Species	D. melanogaster
Name	Bub1 homologue	Annotation symbol	CG14030
Feature type	protein_coding_gene	FlyBase ID	FBgn0031696
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	BubR1
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	25D3-25D3	Sequence location	2L:5,279,053..5,283,283 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Bub1 homologue is referred to in FlyBase by the symbol Dmel\Bub1 (CG14030, FBgn0031696). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: mitotic spindle assembly checkpoint. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Mad3/BUB1 homology region 1; Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1623237_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Bub1. Gene sequence location is 2L:5279051..5283002.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Transcripts Bub1-RB Polypeptides Bub1-PB
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25D3-25D3   Limits computationally determined from genome sequence between P{lacW}tkvk16713&P{lacW}l(2)k01302k01302 and P{lacW}vrik05901&P{PZ}cype03771
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Bub1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.51
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Bub1-RA FBtr0079069  NM_135075   3810   1099 Bub1-RB FBtr0342934     4091   1099
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Bub1-PA   FBpp0078704   125.5   1099   5.91     NP_608919   AAF52236 Bub1-PB   FBpp0309714   125.5   1099   5.91
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Mad3/BUB1 homology region 1 (IPR013212) Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 (IPR015661) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540126   AA698216   AA940715   AE014134 AAF52236   AI543117   AI544381   AI546373   AW942656   AY069508 AAL39653   BE976176   BG638198   BI162483   BI167750   BI170965   BI170966   BI230310   BI234230   BI367045   BI486984   BI619636   BI628876   BI640379   CO154628   CO178299   CO188470   CO265528   CO275887   CO281519   CO287220   CO293704   CO296336   CO306759   CO318868   CO319489   CO321856   CO330723   CO337874   EC051025   EC052012   EC055888   EC058961   EC062914   EC078999   EC197506   EC199427   EC206730   EC207220   EC210244   EC216021   EC219276   EC229699   EC230157   EC236582   EC238946   EC243133   EC247192   EC247622   EC250273   EC254835   ES690050   EV576810
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VMS5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4   organism   • yolk plasma | posterior (Lecuyer et al., 2007) embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 5   pole cell   (Lecuyer et al., 2007) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    128.15 Larval Midgut    45.9 Larval Hindgut    68.3 Larval Malpighian Tubules    87.9 Larval Fat Body    69.3 Larval Salivary Gland    90.9 Larval Trachea    93.85 Larval Carcass    75.15 Adult Head    51.8 Adult Eye    52.725 Adult Brain    101.1 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    85.1 Adult Midgut    80 Adult Hindgut    81.7 Adult Malpighian Tubules    45.6 Adult Fat Body    49.1 Adult Salivary Gland    93.2 Adult Heart    56.15 Adult VirginFemale Spermatheca    33.9 Adult InseminatedFemale Spermatheca    47.3 Adult Ovary    179 Adult Testis    174.2 Adult Male Accessory Gland    59.8 Adult Carcass    52.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    51.8   NA  Eye    52.725   NA  Brain    101.1   128.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    85.6   NA  Crop    85.1   45.9  Midgut    80   68.3  Hindgut    81.7   87.9  Malpighian Tubules    45.6   69.3  Fat Body    49.1   90.9  Salivary Gland    93.2   NA  Heart    56.15   93.85  Trachea    NA   NA  VirginFemale Spermatheca    33.9   NA  InseminatedFemale Spermatheca    47.3   NA  Ovary    179   NA  Testis    174.2   NA  Male Accessory Gland    59.8   75.15  Carcass    52.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (27) central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female  (46) ovary, mated 4-day female  (54) testis, mated 4-day male  (28) accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female  (46) ovary, mated 4-day female  (54) testis, mated 4-day male  28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    27 central nervous system, larvae L3    19 central nervous system, pupae P8    9 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    6 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    5 head, mated 1-day male    9 head, mated 4-day male    12 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    5 digestive system, 4-day adult    9 digestive system, 20-day adult    9 fat body, larvae L3 wandering    5 fat body, white prepupae    13 fat body, pupae P8    2 carcass, larvae L3 wandering    13 carcass, 1-day adult    7 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    46 ovary, mated 4-day female    54 testis, mated 4-day male    28 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (42) embryo 02-04hr  (33) embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (42) embryo 02-04hr  (33) embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    42 embryo 02-04hr    33 embryo 04-06hr    18 embryo 06-08hr    18 embryo 08-10hr    15 embryo 10-12hr    13 embryo 12-14hr    11 embryo 14-16hr    10 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    7 larva L1    8 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    12 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    10 adult male 05day    13 adult male 30day    15 adult female 01day    15 adult female 05day    23 adult female 30day    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1  (28) embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1  (29) CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS  (35) ovary OSC  (37) ovary OSS  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1  28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1  29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS  (35) ovary OSC  (37) ovary OSS  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    12 embryonic 1182-4H    18 embryonic GM2    13 embryonic Kc167    10 embryonic S1    28 embryonic S3    11 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    11 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    12 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    11 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    15 ovary fGS/OSS    35 ovary OSC    37 ovary OSS    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  (34) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (36) Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3  (29) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (30) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (29) Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult  (34) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (36) Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3  29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    15 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    16 Cadmium 100 mM 48 hrs, 4-day adult    34 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    36 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    30 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD22858 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031696 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14030-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 Bub1GD8199 viable Bub1c04512 Sterility Allele fertile Bub1c04512 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Bub1 Class Mutagen Stocks Known lesion Bub1c04512 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Bub1 Class Mutagen Stocks Known lesion Bub1GD8199 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Bub1GL00151 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Bub1KK106893 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Bub1NIG.14030R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Bub1NIG.7838R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Bub1Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6011 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10778} NA P{KK106893} NA P{NIG.7838R} NA P{NIG.14030R} NA P{TRiP.GL00151} NA P{UAS-Bub1.EGFP} NA characterization construct P{BubR1.GFP} NA P{BubR1.mRFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Bub1c04512 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle assembly checkpoint inferred from direct assay (Logarinho et al., 2004)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity with EMBL:AF002823 (FlyBase, 1992-)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Bub1 - AP-2α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - BCAS2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - BubR1 experimental knowledge based (Guruharsha et al., 2011) Bub1 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG13367 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG15220 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG15356 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG17912 experimental knowledge based (Guruharsha et al., 2011) Bub1 - CG32732 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG34163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG3815 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG4619 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG4806 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Cypl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Eb1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Ect4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Nup50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - P32 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - RPA2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - RanGAP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - RpL7-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Rpd3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Tango7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - Tom20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - bor anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - lid anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - mRpL2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - me31B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - nonA-l anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - pont anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - ppan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - rept anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - spoon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - sqh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - tws anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Bub1 - α-Spec anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Bub1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59929 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031696 DroID - A comprehensive database of gene and protein interactions. • FBgn0031696 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33758 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VMS5
Orthologs
OrthoDB Orthologs (31) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG698VK7 Dipteran inclusive ortholog search EOG6640M2 Insect inclusive ortholog search EOG6PG4VQ Arthropod inclusive ortholog search EOG64B8H1 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG698VK7)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Bub1     Drosophila simulans   Dsim\GD23333 Y   Drosophila sechellia   Dsec\GM18544 Y   Drosophila erecta   Dere\GG25065 Y   Drosophila yakuba   Dyak\GE25372 Y   Drosophila ananassae   Dana\GF14386 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA12713 Y   Drosophila persimilis   Dper\GL19568 Y   Drosophila willistoni   Dwil\GK24156 Y   Drosophila virilis   Dvir\GJ17538 Y   Drosophila mojavensis   Dmoj\GI17624 Y   Drosophila grimshawi   Dgri\GH13382 Y
Orthologs in non-Drosophila Dipterans (EOG6640M2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013695
Orthologs in non-Dipteran Insects (EOG6PG4VQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB47414 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014596 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH24345 Y Atta cephalotes Leafcutter ant  Acep\ACEP14802 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17527 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16739 Y Linepithema humile Argentine ant  Lhum\LH19512 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB21753 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20161 Y Solenopsis invicta Red fire ant  Sinv\SINV15368 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001886 Y Bombyx mori Silkmoth  Bmor\BGIBMGA012531 Y Pediculus humanus Human body louse  Phum\PHUM164060 Y Tribolium castaneum Red flour beetle  Tcas\Bub1 Y
Orthologs in non-Insect Arthropods (EOG64B8H1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\OR4F17 Y Daphnia pulex Water flea  Dpux\KRT14 Y Ixodes scapularis Deer tick  Isca\ISCW000858 Y Ixodes scapularis Deer tick  Isca\ISCW000857 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23333 Drosophila sechellia Dsec\GM18544 Drosophila erecta Dere\GG25065 Drosophila yakuba Dyak\GE25372 Drosophila ananassae Dana\GF14386 Drosophila pseudoobscura pseudoobscura Dpse\GA12713 Drosophila persimilis Dper\GL19568 Drosophila willistoni Dwil\GK24156 Drosophila virilis Dvir\GJ17538 Drosophila mojavensis Dmoj\GI17624 Drosophila grimshawi Dgri\GH13382
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	11489 w1118; PBac{PB}Bub1c04512 35260 y1 sc* v1; P{TRiP.GL00151}attP2
Harvard	- PBac{PB}Bub1c04512
VDRC	v101096 P{KK106893}VIE-260B v24833 w1118 P{GD8199}v24833
Genomic Clones ( 2 )
	BACR05M06 BACR25K01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22858
Other clones	DPiM_LD22858
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL03958 LD47933 RE01785 RE08207 RE10102 RE12605 RE12606 RE29896 RE52718 RE70476 RH50711 RH57639 SD09723 SD10921 SD23217
Other clones	bs47g07 Dmel_dig_001798_0589_3646 EC051025 EC052012 EC055888 EK040356 EK056339 EK065238 EK119044 EK142858 EK157349 EK186539 EK204152 EK213415 EK252217 EK274551 EK278354 EK296026 EN04507 EN15502 LD19509
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0031696 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33758
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Bub1 CG14030 (Logarinho et al., 2004)
Source for database merge of
Additional comments	Previous molecular and genetic analysis (FBrf0109303) identified the CG7838 annotation as having significant homology to "Bub1" proteins from other organisms (FlyBase curator comment: this resulted in the "CG7838" gene being renamed to "Bub1" in FlyBase). However, the completion of the Drosophila genome revealed the existence of a second annotation, CG14030, that is also closely related to "Bub1" proteins. Higher eukaryotes also have two genes encoding proteins closely related to "Bub1", one of which retains the symbol "Bub1", while the other is called "BubR1". From phylogenetic analysis including both CG7838 and CG14030 it is difficult to determine unequivocally which gene codes for "Bub1" and which gene codes for "BubR1". However, functional analysis of these proteins in FBrf0174806 suggests that CG14030 encodes the homolog of Bub1 (FlyBase curator comment: as a consequence of this, the gene corresponding to the CG7838 annotation has been renamed "BubR1" and the gene corresponding to the CG14030 annotation has been renamed "Bub1" in FlyBase). (Logarinho et al., 2004)
Other Comments
	dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of Bub1 on the spindle checkpoint. (Muller et al., 2006) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, Aneuploidy, a whole range of mitotic abnormalities, lagging chromatids, central spindle defects are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135075 RefSeq (Proteins) • NP_608919 DDBJ / EMBL / GenBank • AA940715 AAF52236 AAL39653 AW942656 AY069508 Entrez Gene - A searchable database of RefSeq genes. • 33758 UniProtKB/TrEMBL • Q9VMS5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Mad3/BUB1 homology region 1 (IPR013212) Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 (IPR015661) Protein kinase, ATP binding site (IPR017441) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD22858
Linkouts
	BioGRID - A database of protein and genetic interactions • 59929 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0031696 DroID - A comprehensive database of gene and protein interactions. • FBgn0031696 DRSC - Results from RNAi screens. • FBgn0031696 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0031696 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14030-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0031696 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33758 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33758 modMine - Data generated by the modENCODE project. • FBgn0031696 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VMS5
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	Bub1 (Muller et al., 2006, Logarinho et al., 2004, Wen et al., 2008, Schittenhelm et al., 2010, Korenjak et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Althoff et al., 2012) BUB1 (Bettencourt-Dias et al., 2004) bub1 (Somma et al., 2008, Mudiganti et al., 2010) BUB1-like (Morrison et al., 2000) BubR1 (Karess, 2005, Purdy et al., 2005, Graham et al., 2009, Matos et al., 2009, Podhraski et al., 2010) BubRI (Oliveira et al., 2010) CG14030 (Schittenhelm et al., 2009, Logarinho et al., 2004, Lecuyer et al., 2007, Luo et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Japanese National Institute of Genetics, 2012.5.21) CG14030/Bub1 (Bettencourt-Dias et al., 2004)
Name Synonym	Bub1 homologue   CG14030
Secondary FlyBase IDs
References ( 39 )
Generate a list of	All references relating to this gene ( 39 )
List References by type	Research paper  ( 18 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Althoff et al., 2012, Mol. Biol. Cell 23(12): 2275--2291 Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. [FBrf0218626] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...