FlyBase Gene Report: Dmel\Ncoa6

General Information

Symbol

Dmel\Ncoa6

Species

D. melanogaster

Name

Nuclear receptor coactivator 6

Annotation Symbol

CG14023

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031698

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Nuclear receptor coactivator 6 (Ncoa6) encodes a subunit of the Trithorax-related (Trr) complex, a COMPASS-like complex that mediates the methylation of histone H3K4. The product of Ncoa6 is required for transcriptional output of the Hippo pathway by directly interacting with the protein encoded by yki and recruiting the Trr complex to Hippo target genes. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

25D5-25D6

Sequence location

2L:5,315,290..5,326,683 [-]

Recombination map

2-16

RefSeq locus

NT_033779 REGION:5315290..5326683

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables transcription factor binding

inferred from physical interaction with FLYBASE:yki; FB:FBgn0034970

(Qing et al., 2014)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in histone H3-K4 methylation

inferred from direct assay

(Mohan et al., 2011)

involved_in positive regulation of histone H3-K4 methylation

inferred from mutant phenotype

(Qing et al., 2014)

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Oh et al., 2014, Qing et al., 2014)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of MLL3/4 complex

inferred from direct assay

(Mohan et al., 2011)

located_in nucleus

inferred from direct assay

(Qing et al., 2014)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

TRR COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TRR COMPLEX -: The Trithorax-related (Trr) complex is a COMPASS-like complex that mediates the methylation of histone H3K4 and demethylation of histone H3K27. (Adapted from FBrf0216343 and FBrf0218498).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ncoa6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ncoa6-RC

FBtr0307040

NM_135077

9090

2265

Ncoa6-RD

FBtr0307041

NM_001258955

10041

2266

Ncoa6-RF

FBtr0329969

NM_001103618

9905

2467

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ncoa6-PC

FBpp0297883

243.6

2265

10.11

NP_608921

AAF52239

Ncoa6-PD

FBpp0297884

243.7

2266

10.11

NP_001245884

AFH03559

Ncoa6-PF

FBpp0303004

265.4

2467

7.61

NP_001097088

ABV53626

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ncoa6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ncoa6_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of MLL3/4 complex

inferred from direct assay

(Mohan et al., 2011)

located_in nucleus

inferred from direct assay

(Qing et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ncoa6 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ncoa6

View exonic expression by tissue for Dmel\Ncoa6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33761/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031698

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031698

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031698

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14023

Flygut - An atlas of the Drosophila adult midgut

FBgn0031698

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ncoa6

Transgenic constructs containing regulatory region of Ncoa6

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel6012

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ncoa6^GD14752

viable, with Scer\GAL4^Mef2.PR

Ncoa6^GD14752

viable, with Scer\GAL4^pnr-MD237

Ncoa6^GD14752

viable, with Scer\GAL4^tin.CΔ4

Ncoa6^GD14752

Other Phenotypes

Allele

abnormal size | adult stage, with Scer\GAL4^GMR.PF

Ncoa6^GD14752

increased cell death | third instar larval stage

Ncoa6^3PPXA.Tag:HA

visible, with Scer\GAL4^dpp.PU

Ncoa6^HMS00664

visible, with Scer\GAL4^pnr-MD237

Ncoa6^GD14752

visible | adult stage, with Scer\GAL4^en.PU

Ncoa6^GD14752

visible | adult stage, with Scer\GAL4^GMR.PF

Ncoa6^GD14752

Phenotype manifest in

Allele

chaeta | increased number, with Scer\GAL4^pnr-MD237

Ncoa6^GD14752

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Ncoa6^HMS00664

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Ncoa6^HMS00664

wing, with Scer\GAL4^dpp.PU

Ncoa6^HMS00664

wing, with Scer\GAL4^en.PU

Ncoa6^GD14752

wing disc | third instar larval stage

Ncoa6^3PPXA.Tag:HA

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\NCOA6

6 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ncoa6

5 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Ncoa6

3 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\ncoa6

3 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\ncoa6

5 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\mig-38

1 of 15

Yes

Cele\pqn-20

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919017F )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25686

Drosophila persimilis

Dper\GL18557

Drosophila willistoni

Dwil\GK24846

Drosophila virilis

Dvir\GJ21770

Drosophila mojavensis

Dmoj\GI10553

Drosophila grimshawi

Dgri\GH13537

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915008M )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17026668

Glossina morsitans

Tsetse fly

Gmor\GMOY004238

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00661

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NCOA6; nuclear receptor coactivator 6

6 of 15

605299

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ncoa6 - Pa1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mohan et al., 2011)

Ncoa6 - Utx

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mohan et al., 2011)

Ncoa6 - ash2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mohan et al., 2011)

Ncoa6 - yki

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Srivastava et al., 2021, Oh et al., 2014, Qing et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hpo

suppressible

Ncoa6

(Qing et al., 2014)

enhanceable

Ncoa6

(Qing et al., 2014)

wts

suppressible

Ncoa6

(Qing et al., 2014)

yki

suppressible

Ncoa6

(Qing et al., 2014)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031698

MIST (protein-protein) - An integrated Molecular Interaction Database

Ncoa6

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-16

Cytogenetic map

25D5-25D6

Sequence location

2L:5,315,290..5,326,683 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25D5-25D6

Limits computationally determined from genome sequence between P{lacW}vri^k05901&P{PZ}cype⁰³⁷⁷¹ and P{lacW}Hel25E^k11511

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-16

(FlyBase, 2016)

2-17.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Bloomington

18236

w¹¹¹⁸; PBac{RB}Ncoa6^e04200/CyO

40796

y¹ w^*; Mi{MIC}Ncoa6^MI05976

34964

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00664}attP2

Kyoto

114514

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{RB}Ncoa6^e04200 P{neoFRT}40A/CyO y⁺

VDRC

v101908

P{KK110204}VIE-260B

v36480

w¹¹¹⁸; P{GD14752}v36480

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD09783

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031698

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031698

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33761

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Oh et al., 2014)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ncoa6 CG14023

(Mohan et al., 2011)

Source for database merge of

Additional comments

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33761

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031698

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33761/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031698

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ncoa6_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031698

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031698

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14023

Flygut - An atlas of the Drosophila adult midgut

FBgn0031698

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33761

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG14023

MARRVEL_MODEL - MARRVEL (model organism gene)

33761

modMine - A data warehouse for the modENCODE project

FBgn0031698

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031698

DRSC - Results frm RNAi screens

FBgn0031698

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031698

FlyMine - An integrated database for Drosophila genomics

FBgn0031698

MIST (protein-protein) - An integrated Molecular Interaction Database

Ncoa6

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG1402

(Croset et al., 2018)

CG14023

(Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Jungreis et al., 2011, Mohan et al., 2011, Ni et al., 2011.7.19, Zhang et al., 2011, Wasbrough et al., 2010, Zhang et al., 2010, Jiang et al., 2009, Keleman et al., 2009.8.5)

NCOA6

(Cenik and Shilatifard, 2021, Shilatifard, 2012, Schuettengruber et al., 2011)

NcoA6

(Srivastava et al., 2021, Oh et al., 2014)

Ncoa6

(Aboukilila et al., 2020, Chetverina et al., 2020, Tiwari et al., 2019, Zhou et al., 2019, Ma et al., 2018, Piunti and Shilatifard, 2016, Qing et al., 2014)

dNCOA6

(Mohan et al., 2011)

Name Synonyms

Nuclear receptor coactivator 6

(Piunti and Shilatifard, 2016)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (44)

Report Sections

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