FB2020_06, released Dec 22, 2020

Gene: Dmel\Ncoa6

Open Close
General Information
Symbol
Dmel\Ncoa6
Species
D. melanogaster
Name
Nuclear receptor coactivator 6
Annotation Symbol
CG14023
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031698
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
Nuclear receptor coactivator 6 (Ncoa6) encodes a subunit of the Trithorax-related (Trr) complex, a COMPASS-like complex that mediates the methylation of histone H3K4. The product of Ncoa6 is required for transcriptional output of the Hippo pathway by directly interacting with the protein encoded by yki and recruiting the Trr complex to Hippo target genes. [Date last reviewed: 2018-11-15]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
25D5-25D6
Sequence location
2L:5,315,290..5,326,683 [-]
Recombination map

2-16

RefSeq locus
NT_033779 REGION:5315290..5326683
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables transcription factor binding
inferred from physical interaction with FLYBASE:yki; FB:FBgn0034970
(Qing et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in histone H3-K4 methylation
inferred from direct assay
(Mohan et al., 2011)
involved_in positive regulation of histone H3-K4 methylation
inferred from mutant phenotype
(Qing et al., 2014)
involved_in positive regulation of transcription by RNA polymerase II
inferred from direct assay
(Oh et al., 2014, Qing et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of MLL3/4 complex
inferred from direct assay
(Mohan et al., 2011)
located_in nucleus
inferred from direct assay
(Qing et al., 2014)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Group (FlyBase)
    TRR COMPLEX -
    The Trithorax-related (Trr) complex is a COMPASS-like complex that mediates the methylation of histone H3K4 and demethylation of histone H3K27. (Adapted from FBrf0216343 and FBrf0218498).
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    3

    Please see the JBrowse view of Dmel\Ncoa6 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.44

    gene_with_stop_codon_read_through ; SO:0000697

    Stop-codon suppression (UGA) postulated; FBrf0216884.

    Gene model reviewed during 5.55

    Sequence Ontology: Class of Gene
    gene_with_stop_codon_read_through
    (Jungreis et al., 2011)
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Ncoa6-RC
    FBtr0307040
    NM_135077
    9090
    2265
    Ncoa6-RD
    FBtr0307041
    NM_001258955
    10041
    2266
    Ncoa6-RF
    FBtr0329969
    NM_001103618
    9905
    2467
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Ncoa6-PC
    FBpp0297883
    243.6
    2265
    10.11
    NP_608921
    AAF52239
    Ncoa6-PD
    FBpp0297884
    243.7
    2266
    10.11
    NP_001245884
    AFH03559
    Ncoa6-PF
    FBpp0303004
    265.4
    2467
    7.61
    NP_001097088
    ABV53626
    Polypeptides with Identical Sequences

    None of the polypeptides share 100% sequence identity.

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ncoa6 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    mass spectroscopy
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    day 5 of adulthood & male -- day 7 of adulthood & male
    spermatozoon
    (Wasbrough et al., 2010)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    part_of MLL3/4 complex
    inferred from direct assay
    (Mohan et al., 2011)
    located_in nucleus
    inferred from direct assay
    (Qing et al., 2014)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Ncoa6 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 3 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 7 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Ncoa6
    Transgenic constructs containing regulatory region of Ncoa6
    Deletions and Duplications ( 1 )
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Hsap\NCOA6
    6 of 15
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Mmus\Ncoa6
    5 of 15
    Yes
    Yes
    Rattus norvegicus (Norway rat) (1)
    Rnor\Ncoa6
    3 of 13
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (1)
    Xtro\ncoa6
    3 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (1)
    Drer\ncoa6
    5 of 15
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (2)
    Cele\mig-38
    1 of 15
    Yes
    Yes
    Cele\pqn-20
    1 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919017F )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00000245
    Drosophila sechellia
    Dsec\GM18027
    Drosophila sechellia
    Dsec\GM18028
    Drosophila erecta
    Dere\GG24309
    Drosophila yakuba
    Dyak\GE19004
    Drosophila ananassae
    Dana\GF15516
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA25686
    Drosophila persimilis
    Dper\GL18557
    Drosophila willistoni
    Dwil\GK24846
    Drosophila virilis
    Dvir\GJ21770
    Drosophila mojavensis
    Dmoj\GI10553
    Drosophila grimshawi
    Dgri\GH13537
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915008M )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17026668
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY004238
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_00661
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      NCOA6; nuclear receptor coactivator 6
      6 of 15
      605299
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Show neighbor-neighbor interactions:
          Select Layout:
          Legend:
          Protein
          RNA
          Selected Interactor(s)
          Interactions Browser
          View in Interactions Browser
          Please see the Physical Interaction reports below for full details
          protein-protein
          Physical Interaction
          Assay
          References
          Ncoa6 - Pa1
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Mohan et al., 2011)
          Ncoa6 - Utx
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Mohan et al., 2011)
          Ncoa6 - ash2
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Mohan et al., 2011)
          Ncoa6 - yki
          anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot
          (Oh et al., 2014, Qing et al., 2014)
          Summary of Genetic Interactions
          esyN Network Diagram
          esyN Network Key:
          Suppression
          Enhancement
          View in Interactions Browser
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          hpo
          suppressible
          Ncoa6
          (Qing et al., 2014)
          sd
          enhanceable
          Ncoa6
          (Qing et al., 2014)
          wts
          suppressible
          Ncoa6
          (Qing et al., 2014)
          yki
          suppressible
          Ncoa6
          (Qing et al., 2014)
          External Data
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          External Data
          Linkouts
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          2L
          Recombination map

          2-16

          Cytogenetic map
          25D5-25D6
          Sequence location
          2L:5,315,290..5,326,683 [-]
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          25D5-25D6
          Limits computationally determined from genome sequence between P{lacW}vrik05901&P{PZ}cype03771 and P{lacW}Hel25Ek11511
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          2-16

          (FlyBase, 2016)

          2-17.8

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (7)
          Genomic Clones (22)
          cDNA Clones (14)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Oh et al., 2014)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: Ncoa6 CG14023

            (Mohan et al., 2011)
            Source for database merge of
            Additional comments
            Other Comments

            RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

            (Boutros et al., 2004)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 33 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (7)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (40)