FlyBase Gene Report: Dmel\Daxx

Transcription regulator (PubMed:23593018, PubMed:17933869). Acts as a histone chaperone that facilitates deposition of histone H3.3 (PubMed:28320872). Has a role in chromatin remodeling together with asf1 and XNP (PubMed:28320872). Has role in the transcriptional apoptotic response to oxidative and UV stress (PubMed:17933869, PubMed:23593018).

(UniProt, Q9VMD0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Daxx or the JBrowse view of Dmel\Daxx for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Daxx-RA

FBtr0079260

NM_135191

5718

1659

Daxx-RB

FBtr0344764

NM_001298746

5308

1659

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Daxx-PA

FBpp0078890

183.9

1659

10.37

NP_609035

AAF52390

Daxx-PB

FBpp0311092

183.9

1659

10.37

NP_001285675

AHN54190

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1659 aa isoforms: Daxx-PA, Daxx-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with p53 (via C-terminus) (PubMed:17933869). Interacts (via C-terminus) with His3.3A and His3.3B (PubMed:28320872). Interacts with asf1 (PubMed:28320872).

(UniProt, Q9VMD0)

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Daxx using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

DLP_1

Linkouts

Gene Ontology (13 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein binding

inferred from physical interaction with FLYBASE:p53; FB:FBgn0039044

(Bodai et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

histone binding

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription corepressor activity

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription factor binding

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

determination of adult lifespan

inferred from mutant phenotype

(Bodai et al., 2007)

positive regulation of transcription, DNA-templated

inferred from mutant phenotype

(Bodai et al., 2007)

regulation of apoptotic process

inferred from genetic interaction with FLYBASE:p53; FB:FBgn0039044

(Bodai et al., 2007)

response to oxidative stress

inferred from mutant phenotype

(Hwang et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

negative regulation of transcription, DNA-templated

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

nucleosome assembly

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

regulation of apoptotic process

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

cytosol

inferred from direct assay

(assigned by UniProt )

(Hwang et al., 2013)

nucleus

inferred from direct assay

(assigned by UniProt )

(Hwang et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

PML body

inferred from biological aspect of ancestor with PANTHER:PTN000298848

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism | ubiquitous

(Bodai et al., 2007)

embryonic stage 9

organism | restricted

(Bodai et al., 2007)

third instar larval stage

embryonic/larval brain

(Bodai et al., 2007)

wing disc

(Bodai et al., 2007)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Bodai et al., 2007)

Additional Descriptive Data

Daxx transcripts are detected throughout development by qRT-PCR with the highest levels in embryos, third instar larvae, and adult females.

(Bodai et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

cytosol

inferred from direct assay

(assigned by UniProt )

(Hwang et al., 2013)

nucleus

inferred from direct assay

(assigned by UniProt )

(Hwang et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Daxx in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Daxx

View exonic expression by tissue for Dmel\Daxx

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0031820

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9537-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9537

Flygut - An atlas of the Drosophila adult midgut

FBgn0031820

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Daxx

Transgenic constructs containing regulatory region of Daxx

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PI

Daxx^EY09290

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Daxx^GD14768

viable, with Scer\GAL4^Mef2.PR

Daxx^GD14768

viable, with Scer\GAL4^pnr-MD237

Daxx^GD14768

viable, with Scer\GAL4^tin.CΔ4

Daxx^GD14768

Sterility

Allele

female semi-fertile (with Daxx^U26)

Daxx^U32

female semi-fertile (with Daxx^U32)

Daxx^U26

Other Phenotypes

Allele

decreased fecundity | female (with Daxx^U26)

Daxx^U32

decreased fecundity | female (with Daxx^U32)

Daxx^U26

increased cell death, with Scer\GAL4^Bx-MS1096

Daxx^EY09290

oxidative stress response defective

oxidative stress response defective, with Scer\GAL4^elav.PU

short lived

visible, with Scer\GAL4^Bx-MS1096

Daxx^EY09290

Phenotype manifest in

Allele

wing, with Scer\GAL4^Bx-MS1096

Daxx^EY09290

wing disc, with Scer\GAL4^Bx-MS1096

Daxx^EY09290

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DAXX

7 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Daxx

7 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Daxx

6 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\daxx

4 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\daxx

4 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190BAU )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Daxx

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25384

Drosophila persimilis

Dper\GL25670

Drosophila willistoni

Dwil\GK18723

Drosophila virilis

Dvir\GJ18333

Drosophila mojavensis

Dmoj\GI13617

Drosophila grimshawi

Dgri\GH13649

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AW4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018745

Glossina morsitans

Tsetse fly

Gmor\GMOY010094

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12655

Mayetiola destructor

Hessian fly

Mdes\Mdes011229

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL004489

Anopheles darlingi

American malaria mosquito

Adar\ADAC009093

Anopheles gambiae

Malaria mosquito

Agam\Rps21

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001120

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CHJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\LOC100529238

Danaus plexippus

Monarch butterfly

Dple\DPOGS213097-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS204691-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002825

Heliconius melpomene

Postman butterfly

Hmel\HMEL006556

Heliconius melpomene

Postman butterfly

Hmel\HMEL016253

Apis florea

Little honeybee

Aflo\2377248196

Apis mellifera

Western honey bee

Amel\GB45397

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13274

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10540

Bombus terrestris

Buff-tailed bumblebee

Bter\15206631

Linepithema humile

Argentine ant

Lhum\LH12528

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001226

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG015815

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04922

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02403

Tribolium castaneum

Red flour beetle

Tcas\TC009558

Pediculus humanus

Human body louse

Phum\PHUM357210

Rhodnius prolixus

Kissing bug

Rpro\RPRC002976

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI46607

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI21489

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI26485

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_00338

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CFL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009717

Ixodes scapularis

Black-legged tick

Isca\ISCW012819

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80797.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_47165

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0JSL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Daxx

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005773

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Daxx¹

ameliorates Parkinson's disease

modeled by dj-1β^ex54

(Hwang et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DAXX; death domain associated protein

7 of 15

603186

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Daxx - His3.3A

tandem affinity purification, Identification by mass spectrometry, anti tag coimmunoprecipitation, anti tag western blot, western blot

(Fromental-Ramain et al., 2017)

Daxx - His3.3B

anti tag coimmunoprecipitation, anti tag western blot, tandem affinity purification, Identification by mass spectrometry, western blot

(Fromental-Ramain et al., 2017)

Daxx - asf1

anti tag coimmunoprecipitation, western blot

(Fromental-Ramain et al., 2017)

Daxx - p53

pull down, autoradiography, two hybrid

(Lunardi et al., 2010, Bodai et al., 2007)

Daxx - smt3

two hybrid

(Powell et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Daxx

enhanceable

bsk

(Hwang et al., 2013)

Daxx

suppressible

bsk

(Hwang et al., 2013)

Daxx

suppressible

Diap1

(Hwang et al., 2013)

Daxx

suppressible

foxo

(Hwang et al., 2013)

Daxx

enhanceable

hep

(Hwang et al., 2013)

Daxx

suppressible

hep

(Hwang et al., 2013)

Daxx

suppressible

puc

(Hwang et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Daxx

(Schneiderman et al., 2010)

dj-1β

suppressible

Daxx

(Hwang et al., 2013)

p53

enhanceable

Daxx

(Bodai et al., 2007)

External Data

Subunit Structure (UniProtKB)

Interacts with p53 (via C-terminus) (PubMed:17933869). Interacts (via C-terminus) with His3.3A and His3.3B (PubMed:28320872). Interacts with asf1 (PubMed:28320872).

(UniProt, Q9VMD0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60063

DroID - A comprehensive database of gene and protein interactions.

FBgn0031820

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33906

MIST (genetic) - An integrated Molecular Interaction Database

Daxx

MIST (protein-protein) - An integrated Molecular Interaction Database

Daxx

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-20

Cytogenetic map

26D8-26D9

Sequence location

2L:6,480,929..6,487,576 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

26D8-26D9

Limits computationally determined from genome sequence between P{EP}Sec61α^EP2180 and P{lacW}Ate1^k10809

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-20

(FlyBase, 2016)

2-20.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Bloomington

43524

w^*; P{GSV1}Daxx^s-527

24081

w¹¹¹⁸; Mi{ET1}Daxx^MB03646

62353

y¹ v¹; P{TRiP.HMJ23708}attP40/CyO

13741

y¹ w^67c23; P{SUPor-P}Daxx^KG01694

19889

y¹ w^67c23; P{EPgy2}Daxx^EY09290/CyO

79348

y¹ w^*; TI{CRIMIC.TG4.0}Daxx^{CR00895-TG4.0}/SM6a

Kyoto

123995

w¹¹¹⁸; P{RS3}Daxx^CB-6241-3

124399

w¹¹¹⁸; P{RS3}Daxx^UM-8182-3

104777

w^*; P{GawB}Daxx^NP4778 / CyO

200035

y¹ w^67c23; P{GSV1}Sec61α^GS76/SM1

VDRC

v29374

w¹¹¹⁸; P{GD14768}v29374

v29375

w¹¹¹⁸; P{GD14768}v29375/CyO

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (24)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031820

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031820

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33906

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Fromental-Ramain et al., 2017)

polyclonal

(Hwang et al., 2013)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: DLP CG9537

(FlyBase, 1996-)

Source for identity of: Daxx DLP

(FlyBase, 2009-)

Source for database merge of

Additional comments

Renamed from 'DLP' to 'Daxx' (a synonym) to minimise confusion with the 'dlp' ('dally-like protein') gene symbol, from which it differed only by case.

(FlyBase, 2009-)

Source for identity of DLP CG9537 was sequence comparison ( date:011219 ).

(FlyBase, 1996-)

Other Comments

Area matching Drosophila EST AA951193 (inverted).

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33906

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information