FB2025_01 , released February 20, 2025
Gene: Dmel\MICU1
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General Information
Symbol
Dmel\MICU1
Species
D. melanogaster
Name
Mitochondrial calcium uptake 1
Annotation Symbol
CG4495
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031893
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:27568554). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (By similarity). At higher calcium levels, calcium-binding to MICU1 induces a conformational change that weakens MCU-MICU1 interactions and moves MICU1 away from the pore, allowing calcium permeation through the MCU channel (By similarity). Also required to protect against manganese toxicity by preventing manganese uptake by MCU (By similarity). During development, required in alpha/beta or gamma mushroom body neurons to support olfactory intermediate-term memory in the adult (PubMed:27568554). (UniProt, A2VEI2)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
27E4-27E4
Sequence location
Recombination map
2-25
RefSeq locus
NT_033779 REGION:7182234..7185529
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables calcium ion binding
inferred from sequence or structural similarity with UniProtKB:Q9BPX6
(Gene Ontology Curators, 2002-)
inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR039800
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000265479
(GO Reference Genome Project, 2011-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in anesthesia-resistant memory
inferred from mutant phenotype
(Drago and Davis, 2016)
involved_in calcium import into the mitochondrion
inferred from mutant phenotype
(Drago and Davis, 2016)
involved_in medium-term memory
inferred from mutant phenotype
(Drago and Davis, 2016)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in calcium import into the mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000265479
(GO Reference Genome Project, 2011-)
traceable author statement
(Tufi et al., 2019)
involved_in mitochondrial calcium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000265479
(GO Reference Genome Project, 2011-)
involved_in mitochondrial calcium ion transmembrane transport
inferred from electronic annotation with InterPro:IPR039800
(InterPro Project Members, 2004-)
involved_in positive regulation of mitochondrial calcium ion concentration
inferred from sequence or structural similarity with UniProtKB:Q9BPX6
(Gene Ontology Curators, 2002-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of uniplex complex
inferred from biological aspect of ancestor with PANTHER:PTN000265479
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:Q9BPX6
(Gene Ontology Curators, 2002-)
traceable author statement
(Tufi et al., 2019)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MICU1 family. MICU1 subfamily. (A2VEI2)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
MITOCHONDRIAL CALCIUM UNIPORTER COMPLEX -
The mitochondrial calcium uniporter complex is a Ca[2+]-activated channel that mediates the import of Ca[2+] ions from the cytosol into the mitochondrion. (Adapted from FBrf0233624).
Protein Function (UniProtKB)
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:27568554). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (By similarity). At higher calcium levels, calcium-binding to MICU1 induces a conformational change that weakens MCU-MICU1 interactions and moves MICU1 away from the pore, allowing calcium permeation through the MCU channel (By similarity). Also required to protect against manganese toxicity by preventing manganese uptake by MCU (By similarity). During development, required in alpha/beta or gamma mushroom body neurons to support olfactory intermediate-term memory in the adult (PubMed:27568554).
(UniProt, A2VEI2)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\MICU1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A2VEI2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
MICU1-RA
FBtr0079405
NM_135256
1915
524
MICU1-RB
FBtr0113028
NM_001103640
1928
525
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
MICU1-PA
FBpp0079033
60.3
524
8.03
NP_609100
AAF52489
MICU1-PB
FBpp0111941
60.3
525
7.52
A2VEI2
NP_001097110
ABV53641
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain

The EF-hand domains have high affinity for calcium and act as sensors of calcium levels.

(UniProt, A2VEI2)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MICU1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.86

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MICU1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of MICU1
Transgenic constructs containing regulatory region of MICU1
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2L)Exel8019
(Ryder, 2004.4.26)
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (5)
Hsap\MICU1
14 of 14
Yes
Yes
Hsap\MICU2
4 of 14
No
No
Hsap\MICU3
3 of 14
No
No
Hsap\SLC25A12
1 of 14
No
No
Hsap\SLC25A13
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Micu1
14 of 14
Yes
Yes
Rnor\Micu2
4 of 14
No
No
Rnor\Micu3
3 of 14
No
No
Rnor\Slc25a12
1 of 14
No
No
Rnor\Slc25a13
1 of 14
No
No
Mus musculus (laboratory mouse) (5)
Mmus\Micu1
14 of 14
Yes
Yes
Mmus\Micu2
4 of 14
No
No
Mmus\Micu3
2 of 14
No
No
Mmus\Slc25a12
1 of 14
No
No
Mmus\Slc25a13
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\micu1
11 of 13
Yes
Yes
Xtro\zdhhc20
2 of 13
No
No
Xtro\fat1
1 of 13
No
No
Xtro\micu2
1 of 13
No
No
Xtro\micu3
1 of 13
No
No
Xtro\slc25a12
1 of 13
No
No
Xtro\tspan15
1 of 13
No
No
Danio rerio (Zebrafish) (6)
Drer\micu1
14 of 14
Yes
Yes
Drer\micu2
4 of 14
No
Yes
Drer\micu3a
3 of 14
No
No
Drer\micu3b
3 of 14
No
No
Drer\slc25a12
1 of 14
No
No
Drer\slc25a13
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\micu-1
13 of 14
Yes
Yes
Cele\C56A3.6
2 of 14
No
No
Cele\cal-8
1 of 14
No
No
Cele\K02F3.2
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (3)
Agam\AgaP_AGAP009117
12 of 12
Yes
Yes
Agam\AgaP_AGAP004179
2 of 12
No
No
Agam\AgaP_AGAP000958
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\AT4G32060
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:MICU1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
CG4704
9 of 13
MICU3
5 of 13
Aralar
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
MICU1GD4927
model of  myopathy with extrapyramidal signs
CEA
(M'Angale and Staveley, 2017)
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
MICU1; mitochondrial calcium uptake 1
model of  myopathy with extrapyramidal signs
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
MICU1GD4927
model of  myopathy with extrapyramidal signs
is ameliorated by BuffyUAS.cQa
(M'Angale and Staveley, 2017)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
MICU1; mitochondrial calcium uptake 1
14 of 14
605084
MICU2; mitochondrial calcium uptake 2
4 of 14
610632
      MICU3; mitochondrial calcium uptake family member 3
      3 of 14
      610633
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Other Interaction Browsers
          Summary of Genetic Interactions
          esyN Network Diagram
          Show/hide secondary interactors 
          (data from AllianceMine provided by esyN)
          esyN Network Key:
          Suppression
          Enhancement
          Other Interaction Browsers
          View in FlyBase Interactions Browser   View in MIST tool (external link)
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          MICU1
          suppressible
          Buffy
          (M'Angale and Staveley, 2017)
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          MCU|EMRE
          suppressible
          MICU1
          (Tufi et al., 2019)
          External Data
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          FlyBase
          External Links
          External Data
          Linkouts
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Class of Gene
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          2L
          Recombination map
          2-25
          Cytogenetic map
          27E4-27E4
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          27E4-27E4
          Limits computationally determined from genome sequence between P{PZ}snRNP70K02107 and P{PZ}wg02657
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          2-25

          (FlyBase, 2016)

          2-23.6

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (10)
          Genomic Clones (20)
          cDNA Clones (37)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              MICU1 is required in the mushroom body neurons during development for normal intermediate-term olfactory memory in adult flies.

              (Drago and Davis, 2016)
              Relationship to Other Genes
              Source for database merge of
              Additional comments
              Nomenclature History
              Source for database identify of

              Source for identity of: MICU1 CG4495

              (Drago and Davis, 2016)
              Nomenclature comments
              Etymology

              Gene is named "MICU1" based on orthology to human gene.

              (Drago and Davis, 2016)
              Synonyms and Secondary IDs (4)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              Study result (0)
              Result
              Result Type
              Title
              External Crossreferences and Linkouts ( 38 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
              FlyMine - An integrated database for Drosophila genomics
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              MARRVEL_MODEL - MARRVEL (model organism gene)
              Linkouts
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              References (41)