double-stranded RNA-binding protein - bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer, which carrys out the initiation step, to RISC, which carrys out the effector step
Gene model reviewed during 5.52
Low-frequency RNA-Seq exon junction(s) not annotated.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\r2d2 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\r2d2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: r2d2 CG7138
The Dcr-2-r2d2 heterodimer acts as a gatekeeper for the assembly of AGO2 complexes, promoting the incorporation of siRNAs and disfavoring miRNAs as loading substrates for AGO2. A separate mechanism acts in parallel to favor miRNA/miRNA* duplexes and exclude siRNAs from assembly into AGO1 complexes.
r2d2 is not required for repeat-associated small interfering RNA (rasiRNA) production
The orientation of the Dcr-2/r2d2 protein heterodimer on the siRNA duplex determines which siRNA strand associates with the core RISC (RNA-induced silencing complex) protein AGO2. r2d2 protein binds the siRNA end with the greatest double-stranded character, thereby orienting the Dcr-2/r2d2 heterodimer on the siRNA duplex. Strong binding by the r2d2 protein requires a 5'-phosphate on the siRNA strand that is excluded from the RISC.
Dcr-2 protein alone efficiently cleaves dsRNA into siRNA. This enzymatic activity is not affected by association with r2d2 protein. Dcr-2 protein alone does not bind siRNA, but the r2d2/Dcr-2 protein complex binds siRNA and also facilitates the loading of siRNA onto the RNA-initiated silencing complex (RISC). This latter activity is dependent on the dsRNA-binding domains of the r2d2 protein.