FlyBase Gene Report: Dmel\IP3K1

General Information

Symbol

Dmel\IP3K1

Species

D. melanogaster

Name

Inositol 1,4,5-triphosphate kinase 1

Annotation Symbol

CG4026

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032147

Gene Model Status

Current

Stock Availability

26 publicly available

Enzyme Name (EC)

inositol-trisphosphate 3-kinase (2.7.1.127)

Gene Summary

Inositol 1,4,5-triphosphate kinase 1 (IP3K1) encodes a protein involved in response to oxidative stress. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

UY530

Key Links

Genomic Location

Cytogenetic map

30C9-30D1

Sequence location

2L:9,782,384..9,788,499 [+]

Recombination map

2-36

RefSeq locus

NT_033779 REGION:9782384..9788499

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

NOT enables calmodulin binding

inferred from direct assay

(Lloyd-Burton et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables inositol hexakisphosphate kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000274018

(GO Reference Genome Project, 2011-)

enables inositol-1,4,5-trisphosphate 3-kinase activity

inferred from sequence or structural similarity with UniProtKB:Q7KV26

(Seeds et al., 2004)

enables kinase activity

inferred from electronic annotation with InterPro:IPR005522

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in response to oxidative stress

inferred from mutant phenotype

(Monnier et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in inositol phosphate biosynthetic process

inferred from electronic annotation with InterPro:IPR005522

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN004357288

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000274018

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lloyd-Burton et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000963625

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000963625

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

INOSITOL PHOSPHATE KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

inositol-1,4,5-trisphosphate 3-kinase activity

1D-myo-inositol 1,4,5-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5- tetrakisphosphate + ADP + H(+) (2.7.1.127)

RHEA 11020:

Summaries

Gene Snapshot

Inositol 1,4,5-triphosphate kinase 1 (IP3K1) encodes a protein involved in response to oxidative stress. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

INOSITOL PHOSPHATE KINASES -: Inositol phosphate kinases catalyze the phosphorylation of inositol phosphates. (Adapted from PMID:17954248)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\IP3K1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VL83)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

IP3K1-RA

FBtr0079869

NM_135469

2055

441

IP3K1-RB

FBtr0307204

NM_001259025

1417

318

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

IP3K1-PA

FBpp0079465

50.1

441

9.66

FBpp0298033

36.1

318

9.01

M9ND56

NP_001245954

AFH03628

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\IP3K1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.76

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lloyd-Burton et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\IP3K1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\IP3K1

View exonic expression by tissue for Dmel\IP3K1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34300/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032147

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032147

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032147

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4026

Flygut - An atlas of the Drosophila adult midgut

FBgn0032147

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 24 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of IP3K1

Transgenic constructs containing regulatory region of IP3K1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel6025

(Ryder, 2004.4.26)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

IP3K1^KG02192

viable

Sterility

Allele

fertile

IP3K1^EY09888

Other Phenotypes

Allele

abnormal oxidative stress response, with Scer\GAL4^Uro.PT

chemical resistant, with Scer\GAL4^da.G32

chemical sensitive

IP3K1^UY530

Phenotype manifest in

Allele

Malpighian tubule, with Scer\GAL4^Uro.PT

IP3K1^UAS.cMa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

Hsap\ITPKA

8 of 14

Yes

Hsap\ITPKB

8 of 14

Yes

6 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Itpkb

8 of 14

Yes

7 of 14

6 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (7)

Mmus\Itpka

7 of 14

Yes

Mmus\Itpkb

7 of 14

Yes

6 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\LOC100491113

5 of 13

Yes

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (10)

Drer\itpka

7 of 14

Yes

Drer\itpkb

7 of 14

Yes

Drer\si:ch73-22a13.3

7 of 14

Yes

6 of 14

5 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\lfe-2

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP009589

6 of 12

Yes

4 of 12

1 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\IPK2a

2 of 13

Yes

Atha\IPK2BETA

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\ARG82

1 of 13

Yes

Scer\KCS1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC607.04

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:IP3K1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

IP3K2

7 of 13

Ip6k

3 of 13

Ipk2

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

IP3K1^UY530

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ITPKA; inositol-trisphosphate 3-kinase A

8 of 14

147521

ITPKB; inositol-trisphosphate 3-kinase B

8 of 14

147522

ITPKC; inositol-trisphosphate 3-kinase C

6 of 14

606476

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0032147

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of IP3 and IP4 in the cytosol

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synthesis of IP3 and IP4 in the cytosol

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-36

Cytogenetic map

30C9-30D1

Sequence location

2L:9,782,384..9,788,499 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30C9-30D1

Limits computationally determined from genome sequence between P{lacW}zf30C^k02506&P{EP}und^EP424 and P{lacW}l(2)k06416^k06416&P{lacW}sop^k04603

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Maps close to IP3K1 and CG18854.

(Tricoire, 2001.3.6)

Experimentally Determined Recombination Data

Location

2-36

(FlyBase, 2016)

2-31.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (26)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

71993

w¹¹¹⁸; P{VT004958-p65.AD}attP40

72544

w¹¹¹⁸; P{VT004958-GAL4.DBD}attP2

65568

w¹¹¹⁸; PBac{IT.GAL4}IP3K1^1355-G4

37979

y¹ w^*; Mi{MIC}IP3K1^MI04763

19791

y¹ w^67c23; P{EPgy2}IP3K1^EY09888

6769

y¹ w^*; P{Mae-UAS.6.11}IP3K1^UY530

31733

y¹ v¹; P{TRiP.HM04042}attP2/TM3, Sb¹

Korea

13070

P{VT004958-GAL4}attP2

11548

P{EP}IP3K1^G13450

16724

P{VT004958-lexA::GADfl}attP40

NIG-Fly

M2L-1876

y¹ w¹¹¹⁸; IP3K1^SK1 P{neoFRT}40A/CyO-Tb

VDRC

v330538

P{VSH330538}attP40

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (77)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD07279

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032147

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032147

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila ESTs AA697603 and AA801716.

(Davies, 2001.3.15)

Identification: In a screen for mutants resistant to oxidative stress.

(Tricoire et al., 2001)

When overexpressed the mutants show strong resistance to hydrogen peroxide. Loss of function mutants are sensitive to hydrogen peroxide, show a strongly reduced lifespan and present a retinal neurodegenerative phenotype.

(Tricoire et al., 2001)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG4026 UY530

(Monnier et al., 2002)

Source for merge of: IP3K1 anon-WO0118547.189

(FlyBase, 1996-)

Additional comments

"CG4026" may correspond to "Scim15". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim15" near/in "CG4026".

(Dobie and Karpen, 2001.4.18)

Source for merge of IP3K1 anon-WO0118547.189 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG4026

(National Institute of Genetics Fly Stocks, 2022-, Meadows, 2018.4.4, Ben et al., 2011, Ni et al., 2011.7.19, Ni et al., 2008, Lloyd-Burton et al., 2007, Seeds et al., 2004, Monnier et al., 2002, Dobie and Karpen, 2001.4.18, Tricoire, 2001.3.6)

D-IP3K1

(Monnier et al., 2002)

IP3K1

(Li et al., 2025, Geigges et al., 2020, Gene Disruption Project members, 2018-, Prudêncio et al., 2018, Clandinin and Owens, 2016-, Dean et al., 2015, Gene Disruption Project members, 2015-, Sanhueza et al., 2015, Murray et al., 2012, Pezzulo et al., 2012, Ben et al., 2011, Sharma et al., 2011, Gilchrist et al., 2008, Okamura et al., 2008, Buszczak et al., 2007, Gomez-Diaz et al., 2006, Liebl et al., 2006)

IP3KI

(Larsen et al., 2006)

IP₃K-1

(Terhzaz et al., 2010)

Ip3k1

(Martin et al., 2013)

UY530

(Monnier et al., 2002, Tricoire, 2001.3.6)

anon-WO0118547.189

dmIP3Kα

(Seeds et al., 2004)

dmIP₃Kα

(Lloyd-Burton et al., 2007)

Name Synonyms

IP3 kinase 1

(Ben et al., 2011)

Inositol 1,4,5-triphosphate kinase 1

(Monnier et al., 2002)

Inositol 1,4,5-triphosphate kinase1

(Gomez-Diaz et al., 2006)

Secondary FlyBase IDs

FBgn0043966
FBgn0062182

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34300

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VL83

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VL83

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34300/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032147

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032147

FlyMine - An integrated database for Drosophila genomics

FBgn0032147

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4026

MARRVEL_MODEL - MARRVEL (model organism gene)

34300

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032147

DroID - A comprehensive database of gene and protein interactions.

FBgn0032147

DRSC - Results frm RNAi screens

FBgn0032147

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032147

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032147

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4026

Flygut - An atlas of the Drosophila adult midgut

FBgn0032147

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032147

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012691

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synthesis of IP3 and IP4 in the cytosol

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Synthesis of IP3 and IP4 in the cytosol

References (58)

The following external sites may use different assemblies or annotations than FlyBase.

-0.76

Report Sections