FlyBase Gene Report: Dmel\nAChRα6

General Information

Symbol

Dmel\nAChRα6

Species

D. melanogaster

Name

nicotinic Acetylcholine Receptor α6

Annotation Symbol

CG4128

Feature Type

protein_coding_gene

FlyBase ID

FBgn0032151

Gene Model Status

Current

Stock Availability

24 publicly available

Gene Summary

nicotinic Acetylcholine Receptor α6 (nAChRα6) encodes a postsynaptic receptor involved in cation transport, synaptic transmission, muscle homeostasis and sensitivity to insecticides. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Dα6, nAcRalpha-30D, nAcRα-30D, nAChR, nicotinic Acetylcholine Receptor α 30D

Key Links

Genomic Location

Cytogenetic map

30D1-30E1

Sequence location

2L:9,793,317..9,886,250 [-]

Recombination map

2-37

RefSeq locus

NT_033779 REGION:9793317..9886250

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to acetylcholine-gated cation-selective channel activity

inferred from direct assay

(Lansdell et al., 2012, Watson et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables neurotransmitter receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

enables transmembrane signaling receptor activity

inferred from electronic annotation with InterPro:IPR006201

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in cation transport

inferred from direct assay

(Lansdell et al., 2012, Watson et al., 2010)

involved_in muscle cell cellular homeostasis

inferred from mutant phenotype

(Kucherenko et al., 2011)

involved_in response to insecticide

inferred from mutant phenotype

(Watson et al., 2010)

involved_in synaptic transmission, cholinergic

inferred from mutant phenotype

(Watson et al., 2010)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in nervous system process

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of membrane potential

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from direct assay

(Watson et al., 2010)

inferred from physical interaction with FLYBASE:nAChRα5; FB:FBgn0028875, FLYBASE:nAChRα7; FB:FBgn0086778

(Lansdell et al., 2012)

located_in integral component of membrane

inferred from direct assay

(Watson et al., 2010)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in neuron projection

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in postsynaptic membrane

inferred from electronic annotation with InterPro:IPR002394

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

is_active_in synapse

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS -: Nicotinic ligand-gated ion channels (nAChR) mediate the fast actions of the acetylcholine (ACh). The non-selective ion channel is formed from five subunits arranged around a central pore. The ACh binding site is formed by two adjacent α subunits. Other regulatory subunits may modulate channel pharmacology and expression. (Adapted from FBrf0183743).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nAChRα6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.44

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nAChRα6-RA

FBtr0079915

NM_164874

3992

494

nAChRα6-RB

FBtr0079916

NM_205951

3068

494

nAChRα6-RC

FBtr0079914

NM_135472

6723

494

nAChRα6-RD

FBtr0079913

NM_205952

7583

509

nAChRα6-RE

FBtr0079912

NM_205953

6495

523

nAChRα6-RG

FBtr0303898

NM_001201835

7332

494

nAChRα6-RH

FBtr0331284

NM_001273373

3786

494

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

nAChRα6-PA

FBpp0079505

56.1

494

5.88

NP_723494

AAF52817

nAChRα6-PB

FBpp0079506

56.1

494

5.88

NP_995673

AAS64670

nAChRα6-PC

FBpp0079504

56.1

494

5.88

NP_609316

AAN10709

nAChRα6-PD

FBpp0079503

57.9

509

5.73

NP_995674

AAS64672

nAChRα6-PE

FBpp0079502

59.1

523

5.58

NP_995675

AAS64671

nAChRα6-PG

FBpp0292901

56.1

494

5.88

NP_001188764

ADV37014

nAChRα6-PH

FBpp0303720

56.1

494

5.88

NP_001260302

AGB92837

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

494 aa isoforms: nAChRα6-PB, nAChRα6-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nAChRα6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRalpha6_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- pharate adult stage P9

gnathal ganglion | restricted

Comment: 24-72 hpf

(Nakayama et al., 2016)

pupal stage P7 -- pharate adult stage P9

mushroom body calyx | restricted

Comment: 42-72 hpf

(Nakayama et al., 2016)

adult stage

antennal mechanosensory and motor center | faint | restricted

(Nakayama et al., 2016)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from direct assay

(Watson et al., 2010)

inferred from physical interaction with FLYBASE:nAChRα5; FB:FBgn0028875, FLYBASE:nAChRα7; FB:FBgn0086778

(Lansdell et al., 2012)

located_in integral component of membrane

inferred from direct assay

(Watson et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\nAChRα6 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nAChRα6

View exonic expression by tissue for Dmel\nAChRα6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032151

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34304/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032151

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032151

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032151

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4128

Flygut - An atlas of the Drosophila adult midgut

FBgn0032151

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 41 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nAChRα6

Transgenic constructs containing regulatory region of nAChRα6

Aberrations (Deficiencies and Duplications) ( 20 )

Inferred from experimentation ( 20 )

Gene disrupted in

Df(2L)30A-C

(Watson et al., 2010)

Df(2L)Exel6025

(Ryder, 2004.4.26)

Df(2L)γ7

(Watson et al., 2010)

Df(2L)N22-3

(Watson et al., 2010)

Df(2L)N22-5

(Watson et al., 2010)

Df(2L)N22-14

(Watson et al., 2010)

Df(2L)s1402

(Watson et al., 2010)

Gene partially disrupted in

In(2LR)O

(Miller et al., 2018, Perry et al., 2007)

Gene not disrupted in

Gene duplicated in

Dp(2;Y)cb50

(Watson et al., 2010)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

nAChRα6^KK103877

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

nAChRα6^GD1045

viable, with Scer\GAL4^Mef2.PR

nAChRα6^GD1045

viable, with Scer\GAL4^pnr-MD237

nAChRα6^GD1045

viable, with Scer\GAL4^tin.CΔ4

nAChRα6^GD1045

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage

nAChRα6^MB06675

abnormal neurophysiology | larval stage

nAChRα6^DAS1

chemical resistant

chemical resistant (with Df(2L)s1402)

nAChRα6^O

chemical resistant (with nAChRα6^CB1)

nAChRα6^O

chemical resistant (with nAChRα6^CB2)

nAChRα6^O

chemical resistant (with nAChRα6^CB3)

nAChRα6^O

chemical resistant (with nAChRα6^CB4)

nAChRα6^O

chemical resistant (with nAChRα6^CB5)

nAChRα6^O

chemical resistant (with nAChRα6^CB6)

nAChRα6^O

chemical resistant (with nAChRα6^CB7)

nAChRα6^O

chemical resistant (with nAChRα6^CB8)

nAChRα6^O

chemical resistant (with nAChRα6^CB9)

nAChRα6^O

chemical resistant (with nAChRα6^CB10)

nAChRα6^O

chemical resistant (with nAChRα6^CB11)

nAChRα6^O

chemical resistant (with nAChRα6^CB12)

nAChRα6^O

chemical resistant (with nAChRα6^CB13)

nAChRα6^O

chemical resistant (with nAChRα6^CB14)

nAChRα6^O

chemical resistant (with nAChRα6^O)

chemical resistant | adult stage | recessive

nAChRα6^Δ

chemical resistant | semidominant

nAChRα6^G275E

Phenotype manifest in

Allele

indirect flight muscle cell

nAChRα6^EY13897

indirect flight muscle cell (with nAChRα6^EY13897)

nAChRα6^KG05852

indirect flight muscle cell (with nAChRα6^KG05852)

nAChRα6^EY13897

larval ventral nerve cord

nAChRα6^DAS1

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CHRFAM7A

11 of 15

Yes

Hsap\CHRNA7

8 of 15

Yes

Hsap\CHRNA9

1 of 15

Hsap\CHRNA10

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Chrna7

12 of 15

Yes

Mmus\Chrna9

1 of 15

Mmus\Chrna10

1 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Chrna7

8 of 13

Yes

Rnor\Chrna9

1 of 13

Yes

Rnor\Chrna10

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\chrna7

6 of 12

Yes

Xtro\chrna9

1 of 12

Yes

Xtro\chrna10

1 of 12

Yes

Xtro\LOC100487782

1 of 12

Danio rerio (Zebrafish) (9)

Drer\si:ch211-39a7.1

9 of 15

Yes

Drer\chrna7

5 of 15

Drer\LOC101882451

4 of 15

Yes

Drer\si:ch73-380n15.2

4 of 15

Yes

Drer\chrna9

1 of 15

Yes

Drer\chrna10a

1 of 15

Yes

Drer\LOC798522

1 of 15

Yes

Drer\LOC101882637

1 of 15

Yes

Drer\LOC103909988

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (46)

Cele\acr-16

12 of 15

Yes

Cele\acr-11

8 of 15

Cele\acr-19

6 of 15

Yes

5 of 15

5 of 15

4 of 15

4 of 15

4 of 15

4 of 15

4 of 15

2 of 15

Yes

Cele\lgc-12

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Cele\des-2

1 of 15

Cele\lgc-1

1 of 15

Yes

Cele\lgc-4

1 of 15

Yes

Cele\lgc-5

1 of 15

Yes

Cele\lgc-6

1 of 15

Yes

Cele\lgc-7

1 of 15

Yes

Cele\lgc-8

1 of 15

Yes

Cele\lgc-9

1 of 15

Yes

Cele\lgc-10

1 of 15

Yes

Cele\lgc-11

1 of 15

Yes

Cele\lgc-13

1 of 15

Yes

Cele\lgc-14

1 of 15

Yes

Cele\lgc-15

1 of 15

Yes

Cele\lgc-16

1 of 15

Yes

Cele\lgc-17

1 of 15

Yes

Cele\lgc-18

1 of 15

Yes

Cele\lgc-19

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919069Y )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17977

Drosophila persimilis

Dper\GL19206

Drosophila willistoni

Dwil\GK24143

Drosophila virilis

Dvir\GJ14694

Drosophila mojavensis

Dmoj\GI18200

Drosophila grimshawi

Dgri\GH13056

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915058G )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012031

Musca domestica

House fly

Mdoa\17016703

Musca domestica

House fly

Mdoa\17022622

Musca domestica

House fly

Mdoa\17027403

Glossina morsitans

Tsetse fly

Gmor\GMOY006306

Glossina morsitans

Tsetse fly

Gmor\GMOY012039

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10914

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04138

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11281

Mayetiola destructor

Hessian fly

Mdes\Mdes008776

Mayetiola destructor

Hessian fly

Mdes\Mdes008772

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL018352

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008055

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007664

Anopheles darlingi

American malaria mosquito

Adar\ADAC005921

Anopheles darlingi

American malaria mosquito

Adar\ADAC000560

Anopheles gambiae

Malaria mosquito

Agam\alpha5

Anopheles gambiae

Malaria mosquito

Agam\alpha7

Anopheles gambiae

Malaria mosquito

Agam\alpha6

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017758

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04N9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005801

Bombyx mori

Silkmoth

Bmor\achra7

Danaus plexippus

Monarch butterfly

Dple\DPOGS206403-PA

Apis florea

Little honeybee

Aflo\3114357668

Apis florea

Little honeybee

Aflo\2191919204

Apis mellifera

Western honey bee

Amel\GB43416

Apis mellifera

Western honey bee

Amel\GB53053

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20354

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18117

Bombus terrestris

Buff-tailed bumblebee

Bter\15215631

Bombus terrestris

Buff-tailed bumblebee

Bter\15205066

Linepithema humile

Argentine ant

Lhum\LH24959

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000cff

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000c66

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012729

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG016523

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02456

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06697

Tribolium castaneum

Red flour beetle

Tcas\nAChRA7

Tribolium castaneum

Red flour beetle

Tcas\nAChRA6

Pediculus humanus

Human body louse

Phum\PHUM386280

Pediculus humanus

Human body louse

Phum\PHUM385970

Pediculus humanus

Human body louse

Phum\PHUM222790

Rhodnius prolixus

Kissing bug

Rpro\RPRC009466

Rhodnius prolixus

Kissing bug

Rpro\RPRC011267

Cimex lectularius

Bed bug

Clec\CLEC004877

Cimex lectularius

Bed bug

Clec\CLEC004875

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005551

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003469

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_10262

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_10264

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04JR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004751

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56700.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56701.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM66080.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur125g00010

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g02250

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g02270

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g07390

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_321683

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_321682

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0R20 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha4_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha3_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Chrna9_4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_026088gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha2_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Chrna3L_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha9

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha10

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha2_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Chrna9_5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Nacha4

Ciona intestinalis

Vase tunicate

Cint\nAChR

Ciona intestinalis

Vase tunicate

Cint\nachr

Gallus gallus

Domestic chicken

Ggal\CHRNA1

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000007899

Gallus gallus

Domestic chicken

Ggal\CHRNA3

Gallus gallus

Domestic chicken

Ggal\CHRNA4

Gallus gallus

Domestic chicken

Ggal\CHRNA7

Gallus gallus

Domestic chicken

Ggal\CHRNA5

Gallus gallus

Domestic chicken

Ggal\CHRNA10

Gallus gallus

Domestic chicken

Ggal\CHRNA2

Gallus gallus

Domestic chicken

Ggal\CHRNB2

Gallus gallus

Domestic chicken

Ggal\CHRNB4

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000007906

Gallus gallus

Domestic chicken

Ggal\LOC396120

Gallus gallus

Domestic chicken

Ggal\CHRNB3

Gallus gallus

Domestic chicken

Ggal\CHRNA6

Gallus gallus

Domestic chicken

Ggal\CHRNA9

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (22)

Gene Symbol

Score

Source

Alignment

9 of 10

9 of 10

2 of 10

2 of 10

Grd

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Rdl

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

NtR

1 of 10

ort

1 of 10

pHCl-1

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

nAChRα6^KG05852

exacerbates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

nAChRα6^DAS1

exacerbates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

nAChRα6^DAS2

exacerbates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

nAChRα6^{dsRNA.UAS.cUa}

ameliorates neurodegenerative disease

modeled by FIG4^KK102943

(Muraoka et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CHRFAM7A; CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion

11 of 15

609756

CHRNA7; cholinergic receptor nicotinic alpha 7 subunit

8 of 15

118511

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nAChRα6 - RIC-3

anti tag coimmunoprecipitation, autoradiography

(Lansdell et al., 2008)

nAChRα6 - hig

anti tag coimmunoprecipitation, western blot

(Nakayama et al., 2016)

nAChRα6 - nAChRα7

anti tag coimmunoprecipitation, anti tag western blot

(Lansdell and Millar, 2004)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

nAChRα6

(Kucherenko et al., 2008)

Dys

suppressible

nAChRα6

(Kucherenko et al., 2008)

Nf1

enhanceable

nAChRα6

(Walker et al., 2013)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60400

DroID - A comprehensive database of gene and protein interactions.

FBgn0032151

MIST (protein-protein) - An integrated Molecular Interaction Database

nAChRalpha6

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032151

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Highly calcium permeable postsynaptic nicotinic acetylcholine receptors

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-37

Cytogenetic map

30D1-30E1

Sequence location

2L:9,793,317..9,886,250 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30D1-30E1

Limits computationally determined from genome sequence between P{lacW}zf30C^k02506&P{EP}und^EP424 and P{lacW}l(2)k06416^k06416&P{lacW}sop^k04603

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-37

(FlyBase, 2016)

2-32.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (24)

Bloomington

9685

nAChRα6^DAS1

9686

nAChRα6^DAS2

84665

TI{2A-GAL4}nAChRα6^2A-GAL4/CyO

84416

TI{2A-lexA::GAD}nAChRα6^2A-lexA

84542

w^*; TI{TI}nAChRα6^attP/CyO

25835

y¹ v¹; P{TRiP.JF01853}attP2

57818

y¹ v¹; P{TRiP.HMJ21826}attP40/CyO

32058

dpy^ov1 nAChRα6^CB11/CyO, bw¹

37925

y¹ w^*; Mi{MIC}nAChRα6^MI01785

VDRC

v101571

P{KK103877}VIE-260B

v8889

w¹¹¹⁸; P{GD1045}v8889

v8890

w¹¹¹⁸; P{GD1045}v8890

More stocks available...

Genomic Clones (58)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (18)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0032151

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0032151

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34304

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Nakayama et al., 2016, Nakayama et al., 2014, Watson et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: nAcRα-30D CG4128

(FlyBase, 1996-)

Source for identity of: nAChRα6 nAcRα-30D

(FlyBase, 2009-)

Source for database merge of

Source for merge of: nAcRα-30D BcDNA:GH01410

(FlyBase, 1996-)

Additional comments

v(2)k14204 may correspond to nAChRα6: the P{lacW}v(2)k14204^k14204 insertion maps within the transcription unit.

(Gene Disruption Project members, 2015.10.8)

Renamed genes encoding nicotinic Acetylcholine Receptors to give systematic nomenclature that better reflects usage in literature (e.g. FBrf0218556, FBrf0183743).

(FlyBase, 2009-)

Source for identity of nAcRα-30D CG4128 was sequence comparison ( date:020421 ).

(FlyBase, 1996-)

Source for merge of nAcRα-30D BcDNA:GH01410 was a shared cDNA ( date:020730 ).

(FlyBase, 1996-)

Other Comments

nAcRα-30D transcripts undergo A to I pre-mRNA editing, via the Adar gene product.

(Grauso et al., 2002)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 97 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34304

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0032151

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0032151

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34304/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0032151

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRalpha6_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032151

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0032151

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4128

Flygut - An atlas of the Drosophila adult midgut

FBgn0032151

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0032151

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34304

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4128

MARRVEL_MODEL - MARRVEL (model organism gene)

34304

modMine - A data warehouse for the modENCODE project

FBgn0032151

Linkouts

BioGRID - A database of protein and genetic interactions.

60400

DroID - A comprehensive database of gene and protein interactions.

FBgn0032151

DRSC - Results frm RNAi screens

FBgn0032151

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0032151

FlyMine - An integrated database for Drosophila genomics

FBgn0032151

MIST (protein-protein) - An integrated Molecular Interaction Database

nAChRalpha6

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Highly calcium permeable postsynaptic nicotinic acetylcholine receptors

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

AChRα6

(Lyutova et al., 2019)

BcDNA:GH01410

CG4128

(Kondo et al., 2020, Zhang and Zhang, 2019, Transgenic RNAi Project members, 2017-, Tsubouchi et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Jin et al., 2007, Copley, 2004, Tang and Sun, 2002)

CT13662

(Littleton and Ganetzky, 2000)

das

(Savva et al., 2012)

dα6

(Nguyen et al., 2021)

nAChR

(Barber et al., 2021, Rodrigues et al., 2021, Tomizawa et al., 2003, Wolf and Heberlein, 2003)

nAChR alpha 6

(Jin et al., 2007)

nAChR-α30D

(Ren et al., 2012)

nAChRalpha

(Kucherenko et al., 2011)

nAChRalpha6

(Kondo et al., 2020, Rao and Deng, 2019.10.23, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2016-, Gene Disruption Project members, 2015-)

nAChRΑ-30D

(Kucherenko et al., 2008)

nAChRα-30D

(Wolfram et al., 2014)

nAChRα6

(Jin et al., 2021, Tasman et al., 2021, Xie et al., 2021, Yamaguchi et al., 2021, Kurmangaliyev et al., 2020, Mazzotta et al., 2020, Singh et al., 2020, Lopez-Gonzalez et al., 2019, Meltzer et al., 2019, Shih et al., 2019, Croset et al., 2018, Miller et al., 2018, Zhang et al., 2018, Rohde et al., 2016)

nAChrα6

(Douris et al., 2020)

nAcRalpha-30D

(Harbison et al., 2019, Crocker et al., 2016, Aradska et al., 2015, Duff et al., 2015, Ashwal-Fluss et al., 2014, Mackay et al., 2012, Rodriguez et al., 2012, Kucherenko et al., 2011, Cook, 2006.12.5, Giot et al., 2003)

nAcRα-30D

(Ghezzi et al., 2013, Walker et al., 2013, Tsubouchi et al., 2012, Marrone et al., 2011, Ping and Tsunoda, 2011, Takemura et al., 2011, Watson et al., 2010, Yamamoto et al., 2009.2.25, Kucherenko et al., 2008)

nAchR_α6

(Homem et al., 2020)

rsn

(Watson et al., 2010, Cook, 2006.12.5)

α6

(Lansdell et al., 2008)

Name Synonyms

Dα6

(Sattelle et al., 2005)

Nicotinic Acetylcholine Receptor 30D

(Burnette et al., 2005)

nicotinic Acetylcholine Receptor α 30D

(Tsubouchi et al., 2012)

nicotinic Acetylcholine Receptor α6

resistance to spinosyn

(Cook, 2006.12.5)

resistant to spinosyns

(Watson et al., 2010)

α6 nicotinic acetylcholine receptor subunit

(Rinkevich and Scott, 2013)

Secondary FlyBase IDs

FBgn0046810

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (129)

Report Sections

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