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Gene Dmel\RfC3

General Information
Symbol	Dmel\RfC3	Species	D. melanogaster
Name	Replication factor C subunit 3	Annotation symbol	CG5313
Feature type	protein_coding_gene	FlyBase ID	FBgn0032244
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	31E1-31E1	Sequence location	2L:10,419,190..10,420,612 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Replication factor C subunit 3 is referred to in FlyBase by the symbol Dmel\RfC3 (CG5313, FBgn0032244). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: lateral inhibition; neurogenesis. 2 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; chaeta. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, AAA+ type, core; ATPase, AAA-type, core; DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal; Replication factor C. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1637166_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RfC3. Gene sequence location is 2L:10419190..10420612.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN20762 HFA03411
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31E1-31E1   Limits computationally determined from genome sequence between P{lacW}RnrLk06709 and P{lacW}KdelRk00311
Experimentally Determined Cytological Location
Cytogenetic map Notes References 31E-31E     (Shi, 2000.3.22)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RfC3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RfC3-RA FBtr0080019  NM_135555   1134   332
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RfC3-PA   FBpp0079609   37.4   332   7.24     NP_609399   AAF52944
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, AAA+ type, core (IPR003593) ATPase, AAA-type, core (IPR003959) DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (IPR008921) Replication factor C (IPR013748)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52944   AF247499 AAF63387   AY119188 AAM51048   BG640309   BI141886   BP541721   BP559339   BP559340   CO324059   EC067248   EC246670   EC259171   EL882709   EL882710   EV583874
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VKW3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0032244    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0032244 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0032244 expression level Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3175) embryo 02-04hr  (1467) embryo 04-06hr  (3824) embryo 06-08hr  (1936) embryo 08-10hr  (1080) embryo 10-12hr  (571) embryo 12-14hr  (397) embryo 14-16hr  (298) embryo 16-18hr  (208) embryo 18-20hr  (123) embryo 20-22hr  (121) embryo 22-24hr  (150) larva L1  (643) larva L2  (492) larva L3 12hr old  (435) larva L3 puffstage 1-2  (461) larva L3 puffstage 3-6  (632) larva L3 puffstage 7-9  (721) white prepupae new  (524) white prepupae 12hr  (1057) white prepupae 24hr  (797) pupae 2d postWPP  (409) pupae 3d postWPP  (278) pupae 4d postWPP  (247) adult male 01day  (317) adult male 05day  (385) adult male 30day  (363) adult female 01day  (877) adult female 05day  (2018) adult female 30day  (2211) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3175) embryo 02-04hr  1467 embryo 04-06hr  (3824) embryo 06-08hr  (1936) embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day  (2018) adult female 30day  (2211) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0032244 expression level Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3175) embryo 02-04hr  (1467) embryo 04-06hr  (3824) embryo 06-08hr  (1936) embryo 08-10hr  (1080) embryo 10-12hr  (571) embryo 12-14hr  (397) embryo 14-16hr  (298) embryo 16-18hr  (208) embryo 18-20hr  123 embryo 20-22hr  121 embryo 22-24hr  (150) larva L1  (643) larva L2  (492) larva L3 12hr old  (435) larva L3 puffstage 1-2  (461) larva L3 puffstage 3-6  (632) larva L3 puffstage 7-9  (721) white prepupae new  (524) white prepupae 12hr  (1057) white prepupae 24hr  (797) pupae 2d postWPP  (409) pupae 3d postWPP  (278) pupae 4d postWPP  (247) adult male 01day  (317) adult male 05day  (385) adult male 30day  (363) adult female 01day  (877) adult female 05day  (2018) adult female 30day  (2211) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3175) embryo 02-04hr  1467 embryo 04-06hr  (3824) embryo 06-08hr  1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day  2018 adult female 30day  2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3175 embryo 02-04hr    1467 embryo 04-06hr    3824 embryo 06-08hr    1936 embryo 08-10hr    1080 embryo 10-12hr    571 embryo 12-14hr    397 embryo 14-16hr    298 embryo 16-18hr    208 embryo 18-20hr    123 embryo 20-22hr    121 embryo 22-24hr    150 larva L1    643 larva L2    492 larva L3 12hr old    435 larva L3 puffstage 1-2    461 larva L3 puffstage 3-6    632 larva L3 puffstage 7-9    721 white prepupae new    524 white prepupae 12hr    1057 white prepupae 24hr    797 pupae 2d postWPP    409 pupae 3d postWPP    278 pupae 4d postWPP    247 adult male 01day    317 adult male 05day    385 adult male 30day    363 adult female 01day    877 adult female 05day    2018 adult female 30day    2211 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0032244    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0032244 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0032244 expression level Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0032244 expression level Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    178.525 Larval Midgut    23 Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    68.1 Larval Trachea    75.675 Larval Carcass    44.325 Adult Head    20.4 Adult Eye    23.9 Adult Brain    30.5 Adult Thoracic-Abdominal Ganglion    40.5 Adult Crop    21.9 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart    16.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    21.9 Adult Ovary    291.8 Adult Testis    71.3 Adult Male Accessory Gland    100.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    20.4   NA  Eye    23.9   NA  Brain    30.5   178.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    40.5   NA  Crop    21.9   23  Midgut    no informative data   no informative data  Hindgut    no informative data   no informative data  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   68.1  Salivary Gland    no informative data   NA  Heart    16.55   75.675  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    21.9   NA  Ovary    291.8   NA  Testis    71.3   NA  Male Accessory Gland    100.2   44.325  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032244 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5313-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 RfC3GD4599 viable, with Scer\GAL4pnr-MD237 RfC3GD4599 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 RfC3GD4599 visible, with Scer\GAL4pnr-MD237 RfC3GD4599 Phenotype manifest in Allele chaeta, with Scer\GAL4pnr-MD237 RfC3GD4599 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 RfC3GD4599 ganglion mother cell | larval stage, with Scer\GAL41407 RfC3GD4599 trichogen cell, with Scer\GAL4pnr-MD237 RfC3GD4599
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of RfC3 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RfC3 Class Mutagen Stocks Known lesion RfC3GD4599 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes RfC3KK100666 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4599} NA P{KK100666} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR013748 (FlyBase Curators et al., 2004-) DNA binding inferred from sequence or structural similarity (Shi, 2000.3.22) inferred from sequence or structural similarity with Saccharomyces RFC3 (FlyBase, 1992-) DNA clamp loader activity inferred from electronic annotation with InterPro:IPR013748 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA replication inferred from sequence or structural similarity (Shi, 2000.3.22) inferred from sequence or structural similarity with Saccharomyces RFC3 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
DNA replication factor C complex inferred from sequence or structural similarity (Shi, 2000.3.22) inferred from sequence or structural similarity with Saccharomyces RFC3 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RfC3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60505 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0032244 DroID - A comprehensive database of gene and protein interactions. • FBgn0032244 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34423
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15785 Dere\GG10129 Dgri\GH13305 Dmoj\GI18168 Dper\GL19009 Dpse\GA25212 Dsec\GM18361 Dsim\GD23698 Dvir\GJ14606 Dwil\GK15259 Dyak\GE18941
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL009465-PA Aedes aegypti (mosquito) AGAP005144-PA Anopheles gambiae (mosquito) XP_393747.1 Apis mellifera (honey bee) XP_001942989.1 Acyrthosiphon pisum (pea aphid) At1g77470.1 Arabidopsis thaliana (wall cress) 84775 Batrachochytrium dendrobatidis (chytrid fungus) 125553 Branchiostoma floridae (lancelet) 264694 Branchiostoma floridae (lancelet) XP_001899666.1 Brugia malayi (roundworm nematode) BGIBMGA005488-PA Bombyx mori (silk worm) ENSBTAP00000029589 Bos taurus (bovine) CAL0003213 Candida albicans (a form of yeast) CN06871 Caenorhabditis brenneri (small nematode) CBP28594 Caenorhabditis briggsae (nematode) CE37518 Caenorhabditis elegans (worm) ENSCAFP00000014551 Canis familiaris (dog) CAGL0M03575g Candida glabrata (haploid yeast) Chro.30359 Cryptosporidium hominis (obligate parasite) JA11533 Caenorhabditis japonica (nematode) CMO145C Cyanidioschyzon merolae (red alga) cn14062 Cryptococcus neoformans (yeast-like fungus) cgd3_3170 Cryptosporidium parvum (protozoan parasite) ENSCPOP00000016590 Cavia porcellus (guinea pig) 519 Chlamidomonas reinhardtii (green alga) RP13943 Caenorhabditis remanei (nematode) ENSCSAVP00000012898 Ciona savignyi (sedentary tunicate) 164264 Capitella spl (polychaete worm) DDB0232229 Dictyostelium discoideum (amoeba) DEHA2E22286g Debaryomyces Hansenii (yeast) 202808 Daphnia pulex (daphnia) ENSDARP00000051669 Danio rerio (zebra fish) ENSECAP00000013226 Equus caballus (horse) XP_651283 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018743 Gymnopilus aculeatus (mushroom) GL50803_24636 Giardia lamblia (flagellated protozoan) 87902 Hypsipyla robusta (cedar shoot borer) ENSP00000229043 Homo sapiens (human) ISCW022932-PA Ixodes scapularis (deer tick) KLLA0E13201g Kluyveromyces lactis (yeast) 152961 Lottia gigantea (owl limpet) LmjF35.3250 Leishmania major (virus) 30540 Monocosta brevicollis (choanoflagellate) ENSMODP00000019178 Monodelphis domestica (gray opossum) ENSMMUP00000013401 Macaca mulatta (rhesus monkey) ENSMUSP00000083652 Mus musculus (house mouse) 166542 Nematostella vectensis (starlet anemone) XP_001599543.1 Nasonia vitripennis (wasp) ENSOANP00000025138 Ornithorynchus anatinus (platypus) ENSORLP00000001325 Oryzias latipes (japanese killifish) LOC_Os02g53500.1 Oryza sativa (asian rice) 25075 Ostreococcus tauri (green alga) PF14_0601 Plasmodium falciparum (protozoal parasite) PHUM010100-PA Pediculus humanus (human body louse) 136893 Physcomitrella patens (moss) ENSPPYP00000005709 Pongo pygmaeus (orangutan) 71335 Phytophthora ramorum (sudden oak death pathogen) 109673 Phytophthora sojae (root rot pathogen) 816262 Populus trichocarpa (black cottonwood) ENSPTRP00000009355 Pan troglodytes (chimpanzee) PVX_117300 Plasmodium vivax (protozoal parasite) ENSRNOP00000001498 Rattus norvegicus (norway rat) 5055975 Sorghum bicolor (sorghum) YNL290W Saccharomyces cerevisiae (budding yeast) Smp_066260 Schistosoma mansoni (trematode parasite) SPAC27E2.10c Schizosaccharomyces pombe (fission yeast) XP_001195031.1 Strongylocentrotus purpuratus (purple sea urchin) TA08130 Theileria annulata (apicomplexan parasite) Tc00.1047053510661.270 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510737.110 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000018687 Tetraodon nigroviridis (green spotted pufferfish) TP04_0380 Theileria parva (parasitic protozoan) 723 Thalassiosira pseudonana (marine diatom) ENSTRUP00000041746 Takifugu rubripes (pufferfish Takifugu) 17.m00449 Tetrahymena thermophila (ciliate protozoa) 95725.m00217 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000048410 Xenopus tropicalis (frog) YALI0A02068g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15266 NV17901 LH16623 ISCW022932 FBgn0236312 FBgn0217264 FBgn0201813 FBgn0195075 FBgn0173268 JGI_V11_202808 FBgn0246594 FBgn0156610 FBgn0140907 FBgn0120781 FBgn0102440 FBgn0092809 BGIBMGA005488 ACYPI007941 GB19646 AGAP005144 AAEL009465 PHUM473490
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v108001 P{KK100666}VIE-260B v37761 w1118; P{GD4599}v37761 v37762 w1118; P{GD4599}v37762
Genomic Clones ( 2 )
	BACR34C10 BACR48K04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 11 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD11293
Other clones	DPiM_SD11293
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD15852
Other clones	65241 181393 Dmel_dig_014837_0648_2785 EC259171 EK192434 FGC004F11 FGM320D01 IP12412
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0032244 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34423
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RfC3 CG5313 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of RfC3 CG5313 was sequence comparison (date:000414). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135555 RefSeq (Proteins) • NP_609399 DDBJ / EMBL / GenBank • AAF52944 AAF63387 AAM51048 AF247499 AY119188 BG640309 Entrez Gene - A searchable database of RefSeq genes. • 34423 UniProtKB/TrEMBL • Q9VKW3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ATPase, AAA+ type, core (IPR003593) ATPase, AAA-type, core (IPR003959) DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (IPR008921) Replication factor C (IPR013748)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60505 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0032244 DroID - A comprehensive database of gene and protein interactions. • FBgn0032244 DRSC - Results from RNAi screens. • FBgn0032244 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032244 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5313-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0032244 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34423 InParanoid A subset of ortholog calls from InParanoid. • AAEL009465-PA Aedes aegypti (mosquito) AGAP005144-PA Anopheles gambiae (mosquito) XP_393747.1 Apis mellifera (honey bee) XP_001942989.1 Acyrthosiphon pisum (pea aphid) At1g77470.1 Arabidopsis thaliana (wall cress) 84775 Batrachochytrium dendrobatidis (chytrid fungus) 125553 Branchiostoma floridae (lancelet) 264694 Branchiostoma floridae (lancelet) XP_001899666.1 Brugia malayi (roundworm nematode) BGIBMGA005488-PA Bombyx mori (silk worm) ENSBTAP00000029589 Bos taurus (bovine) CAL0003213 Candida albicans (a form of yeast) CN06871 Caenorhabditis brenneri (small nematode) CBP28594 Caenorhabditis briggsae (nematode) CE37518 Caenorhabditis elegans (worm) ENSCAFP00000014551 Canis familiaris (dog) CAGL0M03575g Candida glabrata (haploid yeast) Chro.30359 Cryptosporidium hominis (obligate parasite) JA11533 Caenorhabditis japonica (nematode) CMO145C Cyanidioschyzon merolae (red alga) cn14062 Cryptococcus neoformans (yeast-like fungus) cgd3_3170 Cryptosporidium parvum (protozoan parasite) ENSCPOP00000016590 Cavia porcellus (guinea pig) 519 Chlamidomonas reinhardtii (green alga) RP13943 Caenorhabditis remanei (nematode) ENSCSAVP00000012898 Ciona savignyi (sedentary tunicate) 164264 Capitella spl (polychaete worm) DDB0232229 Dictyostelium discoideum (amoeba) DEHA2E22286g Debaryomyces Hansenii (yeast) 202808 Daphnia pulex (daphnia) ENSDARP00000051669 Danio rerio (zebra fish) ENSECAP00000013226 Equus caballus (horse) XP_651283 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018743 Gymnopilus aculeatus (mushroom) GL50803_24636 Giardia lamblia (flagellated protozoan) 87902 Hypsipyla robusta (cedar shoot borer) ENSP00000229043 Homo sapiens (human) ISCW022932-PA Ixodes scapularis (deer tick) KLLA0E13201g Kluyveromyces lactis (yeast) 152961 Lottia gigantea (owl limpet) LmjF35.3250 Leishmania major (virus) 30540 Monocosta brevicollis (choanoflagellate) ENSMODP00000019178 Monodelphis domestica (gray opossum) ENSMMUP00000013401 Macaca mulatta (rhesus monkey) ENSMUSP00000083652 Mus musculus (house mouse) 166542 Nematostella vectensis (starlet anemone) XP_001599543.1 Nasonia vitripennis (wasp) ENSOANP00000025138 Ornithorynchus anatinus (platypus) ENSORLP00000001325 Oryzias latipes (japanese killifish) LOC_Os02g53500.1 Oryza sativa (asian rice) 25075 Ostreococcus tauri (green alga) PF14_0601 Plasmodium falciparum (protozoal parasite) PHUM010100-PA Pediculus humanus (human body louse) 136893 Physcomitrella patens (moss) ENSPPYP00000005709 Pongo pygmaeus (orangutan) 71335 Phytophthora ramorum (sudden oak death pathogen) 109673 Phytophthora sojae (root rot pathogen) 816262 Populus trichocarpa (black cottonwood) ENSPTRP00000009355 Pan troglodytes (chimpanzee) PVX_117300 Plasmodium vivax (protozoal parasite) ENSRNOP00000001498 Rattus norvegicus (norway rat) 5055975 Sorghum bicolor (sorghum) YNL290W Saccharomyces cerevisiae (budding yeast) Smp_066260 Schistosoma mansoni (trematode parasite) SPAC27E2.10c Schizosaccharomyces pombe (fission yeast) XP_001195031.1 Strongylocentrotus purpuratus (purple sea urchin) TA08130 Theileria annulata (apicomplexan parasite) Tc00.1047053510661.270 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510737.110 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000018687 Tetraodon nigroviridis (green spotted pufferfish) TP04_0380 Theileria parva (parasitic protozoan) 723 Thalassiosira pseudonana (marine diatom) ENSTRUP00000041746 Takifugu rubripes (pufferfish Takifugu) 17.m00449 Tetrahymena thermophila (ciliate protozoa) 95725.m00217 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000048410 Xenopus tropicalis (frog) YALI0A02068g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34423 modMine - Data generated by the modENCODE project. • FBgn0032244 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15266 NV17901 LH16623 ISCW022932 FBgn0236312 FBgn0217264 FBgn0201813 FBgn0195075 FBgn0173268 JGI_V11_202808 FBgn0246594 FBgn0156610 FBgn0140907 FBgn0120781 FBgn0102440 FBgn0092809 BGIBMGA005488 ACYPI007941 GB19646 AGAP005144 AAEL009465 PHUM473490
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG5313 (Krause et al., 2001, Keleman et al., 2009.8.5, Gandhi et al., 2007, Hughes et al., 2008) DmRFC3 (Krause et al., 2001) DRFC (Amin and Li, 2007) RfC3 (Cayirlioglu et al., 2003) Rfc3 (De Lucia et al., 2005)
Name Synonym	Drosophila replication factor C (Amin and Li, 2007) Replication factor C subunit 3   RfC3
Secondary FlyBase IDs
References ( 21 )
Generate a list of	All references relating to this gene ( 21 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...